Rmu_sc0002541.1_g000047

12-oxophytodienoate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002541.1
OG0031213
Physical Location & Seq
Reverse (-)
187150 .. 190471
3322 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1110 bp
atggctaaccaagctcccacacttcctcttctcactccttacaagttgggaaagtttgatctttctcacagagttgttttaccggcattgactagacagagatcatatggatatgttccccagccacctgccatcttatattactctcagagagcatccaaagggggtcttctcataactgaagccactggagtttctgacacagcacaagggtatccagatacccctggtatatggtcaaaggagcaagttgaactgaagcatggaaacccatcgtcaatgctgtacatgctaaagggggtgtcttcttctgtcagatttggcatgtggggagggtttcaaatagtgccagatgggcaagcaccaatctcttctactgacaagtacctaacaccccaaataggatctaatggtattgatgttatgcaattcacacctccaagacgattaaggacagatgaaattcctcaaattgtcaatgatttcagacttgctgcaaggaatgccatggaagctggctttgatggggttgaaattcatggcgccaatggctaccttatagatcagtttttaaaagatcaagtaaatgatcgaacagaccaatatggtggatctctagaaaagcgttgccgatttgcactagacattgttgaagctgttgtcaatgagataggagcagataaagttggagttagattatctccatttgccgactatatggaatctggagattctaatccaaaggaattgggcctttatctagtcaattccttgaacaaatatggaatcctgtactgccacatggttgagccaagaatgaagacacttggacaaaaaattgaatgtccccagagtctcgtacccatgagaaaggcttttaatggtaccttcattgctgctggtggttttgacagggaagatgggaacaatgctgtggctgagggccgtgcagatcttattgcttttggtcgttggttcttggctaatccagatttgccaaagagatttgagcttaatgctcctctaaataagtacatcagagatacattctacatatctgatccggttcttggttacactgattatccatttcttgacagcaccgcttga

Protein Analysis

369

Amino Acids

40.89

Weight (kDa)

5.9

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_FMN PF00724 10 - 342 8.1e-70 NADH:flavin oxidoreductase / NADH oxidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031213)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002541.1_g000047
rosa_wichuraiana Rw0G012540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 136
Acc36I ACCTGC 1 cut(s) 136
Acc65I GGTACC 1 cut(s) 884
AccB1I GGYRCC 2 cut(s) 542, 884
AciI CCGC 1 cut(s) 1104
AclWI GGATC 3 cut(s) 412, 619, 1055
AcsI RAATTY 2 cut(s) 462, 534
AcuI CTGAAG 2 cut(s) 201, 278
AcyI GRCGYC 1 cut(s) 543
AfaI GTAC 6 cut(s) 287, 386, 794, 861, 886, 1034
AfiI CCNNNNNNNGG 2 cut(s) 401, 1070
AgsI TTSAA 6 cut(s) 254, 341, 533, 653, 775, 842
AjnI CCWGG 1 cut(s) 226
AluBI AGCT 4 cut(s) 14, 515, 656, 1012
AluI AGCT 4 cut(s) 14, 515, 656, 1012
Alw26I GTCTC 1 cut(s) 860
AlwI GGATC 3 cut(s) 412, 619, 1055
AoxI GGCC 2 cut(s) 751, 943
ApeKI GCWGC 2 cut(s) 494, 896
ApoI RAATTY 2 cut(s) 462, 534
Asp718I GGTACC 1 cut(s) 884
AspLEI GCGC 1 cut(s) 545
AspS9I GGNCC 2 cut(s) 751, 943
BaeI ACNNNNGTAYC 2 cut(s) 197, 230
BanI GGYRCC 2 cut(s) 542, 884
BbsI GAAGAC 3 cut(s) 161, 297, 827
BbvCI CCTCAGC 1 cut(s) 939
BbvI GCAGC 2 cut(s) 481, 883
BccI CCATC 5 cut(s) 140, 280, 347, 518, 914
BceAI ACGGC 1 cut(s) 930
BciT130I CCWGG 1 cut(s) 228
BciVI GTATCC 1 cut(s) 225
BcoDI GTCTC 1 cut(s) 860
BfaI CTAG 4 cut(s) 93, 617, 641, 761
BfoI RGCGCY 1 cut(s) 546
BfuAI ACCTGC 1 cut(s) 136
BfuI GTATCC 1 cut(s) 225
BglI GCCNNNNNGGC 1 cut(s) 355
BglII AGATCT 1 cut(s) 952
BisI GCNGC 2 cut(s) 495, 897
BlsI GCNGC 2 cut(s) 496, 898
Bme1390I CCNGG 1 cut(s) 228
BmgT120I GGNCC 2 cut(s) 751, 943
BmiI GGNNCC 2 cut(s) 544, 886
BmrFI CCNGG 1 cut(s) 228
BmsI GCATC 1 cut(s) 164
BpiI GAAGAC 3 cut(s) 161, 297, 827
BpmI CTGGAG 2 cut(s) 210, 747
Bpu10I CCTNAGC 1 cut(s) 939
BsaBI GATNNNNATC 1 cut(s) 735
BsaHI GRCGYC 1 cut(s) 543
BsaJI CCNNGG 2 cut(s) 226, 507
BsaWI WCCGGW 1 cut(s) 1063
Bsc4I CCNNNNNNNGG 2 cut(s) 401, 1070
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 1 cut(s) 193
Bse3DI GCAATG 1 cut(s) 891
Bse8I GATNNNNATC 1 cut(s) 735
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 2 cut(s) 226, 507
BseGI GGATG 1 cut(s) 155
BseJI GATNNNNATC 1 cut(s) 735
BseLI CCNNNNNNNGG 2 cut(s) 401, 1070
BseMI GCAATG 1 cut(s) 891
BseMII CTCAG 2 cut(s) 161, 930
BseNI ACTGG 1 cut(s) 193
BseRI GAGGAG 1 cut(s) 1011
BseXI GCAGC 2 cut(s) 481, 883
BseYI CCCAGC 1 cut(s) 120
BsgI GTGCAG 1 cut(s) 969
BshFI GGCC 2 cut(s) 753, 945
BshNI GGYRCC 2 cut(s) 542, 884
BsiSI CCGG 2 cut(s) 83, 1064
BslFI GGGAC 1 cut(s) 831
BslI CCNNNNNNNGG 2 cut(s) 401, 1070
BsmAI GTCTC 1 cut(s) 860
BsmFI GGGAC 1 cut(s) 831
BsmI GAATGC 1 cut(s) 508
BsnI GGCC 2 cut(s) 753, 945
Bsp1407I TGTACA 1 cut(s) 285
Bsp143I GATC 9 cut(s) 58, 101, 404, 562, 577, 589, 611, 952, 1060
Bsp19I CCATGG 1 cut(s) 507
BspACI CCGC 1 cut(s) 1104
BspANI GGCC 2 cut(s) 753, 945
BspCNI CTCAG 2 cut(s) 160, 931
BspLI GGNNCC 2 cut(s) 544, 886
BspMI ACCTGC 1 cut(s) 136
BspPI GGATC 3 cut(s) 412, 619, 1055
BspT107I GGYRCC 2 cut(s) 542, 884
BsrDI GCAATG 1 cut(s) 891
BsrFI RCCGGY 1 cut(s) 82
BsrGI TGTACA 1 cut(s) 285
BsrI ACTGG 1 cut(s) 193
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 2 cut(s) 226, 507
BssMI GATC 9 cut(s) 58, 101, 404, 562, 577, 589, 611, 952, 1060
BssNI GRCGYC 1 cut(s) 543
BssT1I CCWWGG 1 cut(s) 507
Bst2UI CCWGG 1 cut(s) 228
Bst6I CTCTTC 2 cut(s) 33, 376
BstACI GRCGYC 1 cut(s) 543
BstAPI GCANNNNNTGC 1 cut(s) 503
BstAUI TGTACA 1 cut(s) 285
BstC8I GCNNGC 2 cut(s) 360, 517
BstDEI CTNAG 2 cut(s) 147, 939
BstDSI CCRYGG 1 cut(s) 507
BstF5I GGATG 1 cut(s) 155
BstH2I RGCGCY 1 cut(s) 546
BstHHI GCGC 1 cut(s) 545
BstKTI GATC 9 cut(s) 61, 104, 407, 565, 580, 592, 614, 955, 1063
BstMAI GTCTC 1 cut(s) 860
BstMBI GATC 9 cut(s) 58, 101, 404, 562, 577, 589, 611, 952, 1060
BstMWI GCNNNNNNNGC 6 cut(s) 11, 289, 355, 503, 512, 549
BstNI CCWGG 1 cut(s) 228
BstNSI RCATGY 2 cut(s) 292, 328
BstSCI CCNGG 1 cut(s) 226
BstV1I GCAGC 2 cut(s) 481, 883
BstV2I GAAGAC 3 cut(s) 161, 297, 827
BstX2I RGATCY 3 cut(s) 404, 611, 952
BstXI CCANNNNNNTGG 1 cut(s) 608
BstYI RGATCY 3 cut(s) 404, 611, 952
BsuI GTATCC 1 cut(s) 225
BsuRI GGCC 2 cut(s) 753, 945
BtgI CCRYGG 1 cut(s) 507
BtsCI GGATG 1 cut(s) 155
BtsIMutI CAGTG 2 cut(s) 186, 1077
BveI ACCTGC 1 cut(s) 136
Cac8I GCNNGC 2 cut(s) 360, 517
CfoI GCGC 1 cut(s) 545
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 2 cut(s) 751, 943
Csp6I GTAC 6 cut(s) 286, 385, 793, 860, 885, 1033
CviAII CATG 7 cut(s) 263, 289, 325, 508, 539, 802, 865
CviQI GTAC 6 cut(s) 286, 385, 793, 860, 885, 1033
DdeI CTNAG 2 cut(s) 147, 939
DinI GGCGCC 1 cut(s) 544
DpnI GATC 9 cut(s) 60, 103, 406, 564, 579, 591, 613, 954, 1062
DpnII GATC 9 cut(s) 58, 101, 404, 562, 577, 589, 611, 952, 1060
DraI TTTAAA 1 cut(s) 573
Eam1104I CTCTTC 2 cut(s) 33, 376
EarI CTCTTC 2 cut(s) 33, 376
Eco130I CCWWGG 1 cut(s) 507
Eco57I CTGAAG 2 cut(s) 201, 278
EcoRII CCWGG 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 507
EgeI GGCGCC 1 cut(s) 544
EheI GGCGCC 1 cut(s) 544
ErhI CCWWGG 1 cut(s) 507
FaeI CATG 7 cut(s) 266, 292, 328, 511, 542, 805, 868
FalI AAGNNNNNCTT 2 cut(s) 153, 185
FaqI GGGAC 1 cut(s) 831
FatI CATG 7 cut(s) 262, 288, 324, 507, 538, 801, 864
FauNDI CATATG 1 cut(s) 106
Fnu4HI GCNGC 2 cut(s) 495, 897
FokI GGATG 1 cut(s) 142
Fsp4HI GCNGC 2 cut(s) 495, 897
FspBI CTAG 4 cut(s) 93, 617, 641, 761
GlaI GCGC 1 cut(s) 544
GluI GCNGC 2 cut(s) 495, 897
GsaI CCCAGC 1 cut(s) 124
GsuI CTGGAG 2 cut(s) 210, 747
HaeII RGCGCY 1 cut(s) 546
HaeIII GGCC 2 cut(s) 753, 945
HapII CCGG 2 cut(s) 83, 1064
HhaI GCGC 1 cut(s) 545
Hin1I GRCGYC 1 cut(s) 543
Hin1II CATG 7 cut(s) 266, 292, 328, 511, 542, 805, 868
Hin6I GCGC 1 cut(s) 543
HinP1I GCGC 1 cut(s) 543
HinfI GANTC 4 cut(s) 722, 731, 786, 853
HpaII CCGG 2 cut(s) 83, 1064
Hpy188I TCNGA 6 cut(s) 150, 199, 317, 488, 1040, 1060
Hpy188III TCNNGA 5 cut(s) 218, 617, 726, 989, 1094
HpyAV CCTTC 1 cut(s) 898
HpyCH4V TGCA 4 cut(s) 427, 497, 638, 950
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 289, 355, 503, 512, 549
HpyF3I CTNAG 2 cut(s) 147, 939
Hsp92I GRCGYC 1 cut(s) 543
Hsp92II CATG 7 cut(s) 266, 292, 328, 511, 542, 805, 868
HspAI GCGC 1 cut(s) 543
KasI GGCGCC 1 cut(s) 542
KpnI GGTACC 1 cut(s) 888
Kzo9I GATC 9 cut(s) 58, 101, 404, 562, 577, 589, 611, 952, 1060
LmnI GCTCC 4 cut(s) 19, 244, 674, 1024
Lsp1109I GCAGC 2 cut(s) 481, 883
LweI GCATC 1 cut(s) 164
MaeI CTAG 4 cut(s) 93, 617, 641, 761
MaeIII GTNAC 1 cut(s) 1073
MalI GATC 9 cut(s) 60, 103, 406, 564, 579, 591, 613, 954, 1062
MboI GATC 9 cut(s) 58, 101, 404, 562, 577, 589, 611, 952, 1060
MboII GAAGA 7 cut(s) 20, 161, 297, 300, 363, 832, 929
MflI RGATCY 3 cut(s) 404, 611, 952
MluCI AATT 7 cut(s) 428, 462, 471, 534, 746, 766, 837
Mly113I GGCGCC 1 cut(s) 543
MlyI GAGTC 1 cut(s) 862
MmeI TCCRAC 1 cut(s) 667
MnlI CCTC 6 cut(s) 36, 326, 447, 477, 934, 1032
MseI TTAA 4 cut(s) 449, 572, 879, 1014
MspI CCGG 2 cut(s) 83, 1064
MspR9I CCNGG 1 cut(s) 228
Mva1269I GAATGC 1 cut(s) 508
MvaI CCWGG 1 cut(s) 228
MwoI GCNNNNNNNGC 6 cut(s) 11, 289, 355, 503, 512, 549
NarI GGCGCC 1 cut(s) 543
NcoI CCATGG 1 cut(s) 507
NdeI CATATG 1 cut(s) 106
NdeII GATC 9 cut(s) 58, 101, 404, 562, 577, 589, 611, 952, 1060
NlaIII CATG 7 cut(s) 266, 292, 328, 511, 542, 805, 868
NlaIV GGNNCC 2 cut(s) 544, 886
NspI RCATGY 2 cut(s) 292, 328
PaqCI CACCTGC 1 cut(s) 136
PctI GAATGC 1 cut(s) 508
PfeI GAWTC 3 cut(s) 722, 731, 786
PkrI GCNGC 2 cut(s) 496, 898
PleI GAGTC 1 cut(s) 861
PluTI GGCGCC 1 cut(s) 546
PpsI GAGTC 1 cut(s) 861
Psp6I CCWGG 1 cut(s) 226
PspFI CCCAGC 1 cut(s) 120
PspGI CCWGG 1 cut(s) 226
PspN4I GGNNCC 2 cut(s) 544, 886
PspPI GGNCC 2 cut(s) 751, 943
PsuI RGATCY 3 cut(s) 404, 611, 952
RsaI GTAC 6 cut(s) 287, 386, 794, 861, 886, 1034
RsaNI GTAC 6 cut(s) 286, 385, 793, 860, 885, 1033
SaqAI TTAA 4 cut(s) 449, 572, 879, 1014
SatI GCNGC 2 cut(s) 495, 897
Sau3AI GATC 9 cut(s) 58, 101, 404, 562, 577, 589, 611, 952, 1060
Sau96I GGNCC 2 cut(s) 751, 943
SchI GAGTC 1 cut(s) 862
ScrFI CCNGG 1 cut(s) 228
SetI ASST 9 cut(s) 16, 130, 390, 439, 517, 558, 658, 890, 1014
SfaNI GCATC 1 cut(s) 164
SfoI GGCGCC 1 cut(s) 544
Sse9I AATT 7 cut(s) 428, 462, 471, 534, 746, 766, 837
SsiI CCGC 1 cut(s) 1104
SspDI GGCGCC 1 cut(s) 542
SspMI CTAG 4 cut(s) 93, 617, 641, 761
StyD4I CCNGG 1 cut(s) 226
StyI CCWWGG 1 cut(s) 507
TaqI TCGA 1 cut(s) 592
TasI AATT 7 cut(s) 428, 462, 471, 534, 746, 766, 837
TatI WGTACW 3 cut(s) 285, 792, 1032
TfiI GAWTC 3 cut(s) 722, 731, 786
Tru1I TTAA 4 cut(s) 449, 572, 879, 1014
Tru9I TTAA 4 cut(s) 449, 572, 879, 1014
TscAI CASTG 2 cut(s) 193, 1084
TseI GCWGC 2 cut(s) 494, 896
TspDTI ATGAA 4 cut(s) 474, 527, 833, 880
TspRI CASTG 2 cut(s) 193, 1084
XapI RAATTY 2 cut(s) 462, 534
XbaI TCTAGA 1 cut(s) 616
XceI RCATGY 2 cut(s) 292, 328
XspI CTAG 4 cut(s) 93, 617, 641, 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.