Rw0G012540

12-oxophytodienoate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00560
OG0031213
Physical Location & Seq
Reverse (-)
33222 .. 36575
3354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G012540.1

Sequence Viewer

Length: 1194 bp
ATGGCTAACCAAGCTCCCACACTTCCTCTTCTCACTCCTTACAAGTTGGGAAAGTTTGATCTTTCTCACAGAGTTGTTTTACCGGCATTGACTAGACAGAGATCATATGGATATGTTCCCCAGCCACCTGCCATCTTATATTACTCTCAGAGAGCATCCAAAGGGGGTCTTCTCATAACTGAAGCCACTGGAGTTTCTGACACAGCACAAGGGTATCCAGATACCCCTGGTATATGGTCAAAGGAGCAAGTTGAACTGAAGCATGGAAACCCATCGTCAATGCTGTACATGCTAAAGGGGGTGTCTTCTTCTGTCAGATTTGGCATGTGCGGAGGGTTTCAAATAGTGGTGCGCTTTCAGCCAGATGGGCAAGCACCAATCTCTTCTACTGACAAGTACCTAACACCCCAAATAGGATCTAATGGTATTGATGTTATGCAATTCACACCTCCAAGACGATTAAGGGCAGATGAAATTCCTCAAATTGTCAATGATTTCAGACTTGCTGCAAGGAATGCTATGGAAGCTGGCTTTGATGGAGTTGAAATTCATGGCTCCAGTGGCTTCCTTATCGATCAGTTTTTAAAAGATCAAGTAAATGATCGAACAGACCAATATGGTGGATCTCTAGAAAGGCGTTGCCGATTTGCACTAGACATTGTTGAAGCTGTTGTCAACGAGATAGGAGCAGATAAAGTTGGAATTAAATTATCTCCATTTGCCAACTATATGGAATCCGGAGATTCTAAACCAAAGGAATTGGGCCTTTATCTAGTCAATTCCTTGAACAAATATGGAATTCTGTACTGCCGAATGATTGAGCCAAGAATGAAGACACTTGGAGACAAAATTGAATGTCCCCAGAGTCTTGTACCCATGAGAAAGGCTTTTAATGGTACTTTCATTGCTGTTGGCGGTTTTGACAGGGAAGATGGGAACAATGCTGTGGCTGAAGACAGTGCAGATCTTGTTGCTTTTGGTCGTTGGTTCTTGGCTAATCCAGATTTGCCAAAGAGATTTGAGCTTAATGCTCCTCTAAATAAGTACATCAGAGATACATTCTACATATCTGATCCCGTTCTTGGTTACACTAATTATCCATTCTACAATAAAAAAAAATGTCAAAAGACCTTGTTGGAACTACTGGATTTCTGTTGTGTGTATCTGAACTTTTCTGTTCACGTTAACTTGTAA

Protein Analysis

397

Amino Acids

44.3

Weight (kDa)

8.03

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_FMN PF00724 10 - 346 5.7e-65 NADH:flavin oxidoreductase / NADH oxidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031213)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002541.1_g000047
rosa_wichuraiana Rw0G012540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 136
Acc36I ACCTGC 1 cut(s) 136
AccIII TCCGGA 1 cut(s) 737
AciI CCGC 2 cut(s) 330, 915
AclWI GGATC 3 cut(s) 424, 631, 1067
AcsI RAATTY 3 cut(s) 474, 546, 798
AcuI CTGAAG 3 cut(s) 201, 278, 972
AfaI GTAC 6 cut(s) 287, 398, 806, 873, 898, 1046
AfiI CCNNNNNNNGG 2 cut(s) 413, 1082
AgsI TTSAA 6 cut(s) 254, 341, 545, 665, 787, 854
AjnI CCWGG 1 cut(s) 226
AluBI AGCT 4 cut(s) 14, 527, 668, 1024
AluI AGCT 4 cut(s) 14, 527, 668, 1024
Alw26I GTCTC 1 cut(s) 837
AlwI GGATC 3 cut(s) 424, 631, 1067
Aor13HI TCCGGA 1 cut(s) 737
AoxI GGCC 1 cut(s) 763
ApeKI GCWGC 1 cut(s) 506
ApoI RAATTY 3 cut(s) 474, 546, 798
AspLEI GCGC 1 cut(s) 354
AspS9I GGNCC 1 cut(s) 763
BaeI ACNNNNGTAYC 2 cut(s) 197, 230
BbsI GAAGAC 4 cut(s) 161, 297, 839, 960
BbvI GCAGC 1 cut(s) 493
BccI CCATC 5 cut(s) 140, 280, 359, 530, 926
BciT130I CCWGG 1 cut(s) 228
BciVI GTATCC 1 cut(s) 225
BcoDI GTCTC 1 cut(s) 837
BfaI CTAG 4 cut(s) 93, 629, 653, 773
BfuAI ACCTGC 1 cut(s) 136
BfuI GTATCC 1 cut(s) 225
BglI GCCNNNNNGGC 1 cut(s) 367
BglII AGATCT 1 cut(s) 964
BisI GCNGC 1 cut(s) 507
BlsI GCNGC 1 cut(s) 508
Bme1390I CCNGG 1 cut(s) 228
BmgT120I GGNCC 1 cut(s) 763
BmiI GGNNCC 1 cut(s) 556
BmrFI CCNGG 1 cut(s) 228
BmsI GCATC 1 cut(s) 164
BpiI GAAGAC 4 cut(s) 161, 297, 839, 960
BpmI CTGGAG 2 cut(s) 210, 541
Bsa29I ATCGAT 1 cut(s) 573
BsaJI CCNNGG 1 cut(s) 226
BsaWI WCCGGW 1 cut(s) 737
Bsc4I CCNNNNNNNGG 2 cut(s) 413, 1082
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 3 cut(s) 193, 558, 1149
Bse3DI GCAATG 1 cut(s) 903
BseAI TCCGGA 1 cut(s) 737
BseBI CCWGG 1 cut(s) 228
BseCI ATCGAT 1 cut(s) 573
BseDI CCNNGG 1 cut(s) 226
BseGI GGATG 1 cut(s) 155
BseLI CCNNNNNNNGG 2 cut(s) 413, 1082
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 1 cut(s) 161
BseNI ACTGG 3 cut(s) 193, 558, 1149
BseRI GAGGAG 1 cut(s) 1023
BseXI GCAGC 1 cut(s) 493
BseYI CCCAGC 1 cut(s) 120
BsgI GTGCAG 1 cut(s) 981
BshFI GGCC 1 cut(s) 765
BshVI ATCGAT 1 cut(s) 573
BsiSI CCGG 2 cut(s) 83, 738
BslFI GGGAC 1 cut(s) 843
BslI CCNNNNNNNGG 2 cut(s) 413, 1082
BsmAI GTCTC 1 cut(s) 837
BsmFI GGGAC 1 cut(s) 843
BsmI GAATGC 1 cut(s) 520
BsnI GGCC 1 cut(s) 765
Bsp13I TCCGGA 1 cut(s) 737
Bsp1407I TGTACA 1 cut(s) 285
Bsp143I GATC 9 cut(s) 58, 101, 416, 574, 589, 601, 623, 964, 1072
BspACI CCGC 2 cut(s) 330, 915
BspANI GGCC 1 cut(s) 765
BspCNI CTCAG 1 cut(s) 160
BspDI ATCGAT 1 cut(s) 573
BspEI TCCGGA 1 cut(s) 737
BspLI GGNNCC 1 cut(s) 556
BspMI ACCTGC 1 cut(s) 136
BspPI GGATC 3 cut(s) 424, 631, 1067
BsrDI GCAATG 1 cut(s) 903
BsrFI RCCGGY 1 cut(s) 82
BsrGI TGTACA 1 cut(s) 285
BsrI ACTGG 3 cut(s) 193, 558, 1149
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 1 cut(s) 226
BssMI GATC 9 cut(s) 58, 101, 416, 574, 589, 601, 623, 964, 1072
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 1 cut(s) 959
Bst6I CTCTTC 2 cut(s) 33, 388
BstAPI GCANNNNNTGC 1 cut(s) 515
BstAUI TGTACA 1 cut(s) 285
BstC8I GCNNGC 2 cut(s) 372, 529
BstDEI CTNAG 1 cut(s) 147
BstF5I GGATG 1 cut(s) 155
BstHHI GCGC 1 cut(s) 354
BstKTI GATC 9 cut(s) 61, 104, 419, 577, 592, 604, 626, 967, 1075
BstMAI GTCTC 1 cut(s) 837
BstMBI GATC 9 cut(s) 58, 101, 416, 574, 589, 601, 623, 964, 1072
BstMWI GCNNNNNNNGC 7 cut(s) 11, 289, 358, 367, 515, 524, 561
BstNI CCWGG 1 cut(s) 228
BstNSI RCATGY 2 cut(s) 292, 328
BstSCI CCNGG 1 cut(s) 226
BstV1I GCAGC 1 cut(s) 493
BstV2I GAAGAC 4 cut(s) 161, 297, 839, 960
BstX2I RGATCY 3 cut(s) 416, 623, 964
BstXI CCANNNNNNTGG 2 cut(s) 620, 730
BstYI RGATCY 3 cut(s) 416, 623, 964
Bsu15I ATCGAT 1 cut(s) 573
BsuI GTATCC 1 cut(s) 225
BsuRI GGCC 1 cut(s) 765
BsuTUI ATCGAT 1 cut(s) 573
BtsCI GGATG 1 cut(s) 155
BtsIMutI CAGTG 3 cut(s) 186, 565, 964
BveI ACCTGC 1 cut(s) 136
Cac8I GCNNGC 2 cut(s) 372, 529
CfoI GCGC 1 cut(s) 354
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 1 cut(s) 763
ClaI ATCGAT 1 cut(s) 573
Csp6I GTAC 6 cut(s) 286, 397, 805, 872, 897, 1045
CviAII CATG 5 cut(s) 263, 289, 325, 551, 877
CviQI GTAC 6 cut(s) 286, 397, 805, 872, 897, 1045
DdeI CTNAG 1 cut(s) 147
DpnI GATC 9 cut(s) 60, 103, 418, 576, 591, 603, 625, 966, 1074
DpnII GATC 9 cut(s) 58, 101, 416, 574, 589, 601, 623, 964, 1072
DraI TTTAAA 1 cut(s) 585
Eam1104I CTCTTC 2 cut(s) 33, 388
EarI CTCTTC 2 cut(s) 33, 388
Eco57I CTGAAG 3 cut(s) 201, 278, 972
EcoRI GAATTC 1 cut(s) 798
EcoRII CCWGG 1 cut(s) 226
FaeI CATG 5 cut(s) 266, 292, 328, 554, 880
FalI AAGNNNNNCTT 2 cut(s) 153, 185
FaqI GGGAC 1 cut(s) 843
FatI CATG 5 cut(s) 262, 288, 324, 550, 876
FauNDI CATATG 1 cut(s) 106
Fnu4HI GCNGC 1 cut(s) 507
FokI GGATG 1 cut(s) 142
Fsp4HI GCNGC 1 cut(s) 507
FspBI CTAG 4 cut(s) 93, 629, 653, 773
GlaI GCGC 1 cut(s) 353
GluI GCNGC 1 cut(s) 507
GsaI CCCAGC 1 cut(s) 124
GsuI CTGGAG 2 cut(s) 210, 541
HaeIII GGCC 1 cut(s) 765
HapII CCGG 2 cut(s) 83, 738
HhaI GCGC 1 cut(s) 354
Hin1II CATG 5 cut(s) 266, 292, 328, 554, 880
Hin6I GCGC 1 cut(s) 352
HinP1I GCGC 1 cut(s) 352
HincII GTYRAC 2 cut(s) 676, 1186
HindII GTYRAC 2 cut(s) 676, 1186
HinfI GANTC 3 cut(s) 734, 743, 865
HpaI GTTAAC 1 cut(s) 1186
HpaII CCGG 2 cut(s) 83, 738
Hpy166II GTNNAC 3 cut(s) 676, 1180, 1186
Hpy188I TCNGA 7 cut(s) 150, 199, 317, 500, 1052, 1072, 1167
Hpy188III TCNNGA 4 cut(s) 218, 629, 738, 1001
Hpy8I GTNNAC 3 cut(s) 676, 1180, 1186
HpyCH4III ACNGT 1 cut(s) 959
HpyCH4IV ACGT 1 cut(s) 1182
HpyCH4V TGCA 4 cut(s) 439, 509, 650, 962
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 289, 358, 367, 515, 524, 561
HpyF3I CTNAG 1 cut(s) 147
HpySE526I ACGT 1 cut(s) 1182
Hsp92II CATG 5 cut(s) 266, 292, 328, 554, 880
HspAI GCGC 1 cut(s) 352
Kpn2I TCCGGA 1 cut(s) 737
KspAI GTTAAC 1 cut(s) 1186
Kzo9I GATC 9 cut(s) 58, 101, 416, 574, 589, 601, 623, 964, 1072
LmnI GCTCC 5 cut(s) 19, 244, 560, 686, 1036
Lsp1109I GCAGC 1 cut(s) 493
LweI GCATC 1 cut(s) 164
MaeI CTAG 4 cut(s) 93, 629, 653, 773
MaeII ACGT 1 cut(s) 1182
MaeIII GTNAC 1 cut(s) 1085
MalI GATC 9 cut(s) 60, 103, 418, 576, 591, 603, 625, 966, 1074
MboI GATC 9 cut(s) 58, 101, 416, 574, 589, 601, 623, 964, 1072
MboII GAAGA 8 cut(s) 20, 161, 297, 300, 375, 844, 941, 965
MflI RGATCY 3 cut(s) 416, 623, 964
MlyI GAGTC 1 cut(s) 874
MmeI TCCRAC 2 cut(s) 679, 1116
MnlI CCTC 5 cut(s) 36, 326, 459, 489, 1044
MroI TCCGGA 1 cut(s) 737
MseI TTAA 6 cut(s) 461, 584, 705, 891, 1026, 1185
MspI CCGG 2 cut(s) 83, 738
MspR9I CCNGG 1 cut(s) 228
Mva1269I GAATGC 1 cut(s) 520
MvaI CCWGG 1 cut(s) 228
MwoI GCNNNNNNNGC 7 cut(s) 11, 289, 358, 367, 515, 524, 561
NdeI CATATG 1 cut(s) 106
NdeII GATC 9 cut(s) 58, 101, 416, 574, 589, 601, 623, 964, 1072
NlaIII CATG 5 cut(s) 266, 292, 328, 554, 880
NlaIV GGNNCC 1 cut(s) 556
NspI RCATGY 2 cut(s) 292, 328
PaqCI CACCTGC 1 cut(s) 136
PctI GAATGC 1 cut(s) 520
PfeI GAWTC 2 cut(s) 734, 743
PkrI GCNGC 1 cut(s) 508
PleI GAGTC 1 cut(s) 873
PpsI GAGTC 1 cut(s) 873
Psp6I CCWGG 1 cut(s) 226
PspFI CCCAGC 1 cut(s) 120
PspGI CCWGG 1 cut(s) 226
PspN4I GGNNCC 1 cut(s) 556
PspPI GGNCC 1 cut(s) 763
PsuI RGATCY 3 cut(s) 416, 623, 964
RsaI GTAC 6 cut(s) 287, 398, 806, 873, 898, 1046
RsaNI GTAC 6 cut(s) 286, 397, 805, 872, 897, 1045
SaqAI TTAA 6 cut(s) 461, 584, 705, 891, 1026, 1185
SatI GCNGC 1 cut(s) 507
Sau3AI GATC 9 cut(s) 58, 101, 416, 574, 589, 601, 623, 964, 1072
Sau96I GGNCC 1 cut(s) 763
SchI GAGTC 1 cut(s) 874
ScrFI CCNGG 1 cut(s) 228
SetI ASST 9 cut(s) 16, 130, 402, 451, 529, 670, 1026, 1133, 1185
SfaNI GCATC 1 cut(s) 164
SsiI CCGC 2 cut(s) 330, 915
SspMI CTAG 4 cut(s) 93, 629, 653, 773
StyD4I CCNGG 1 cut(s) 226
TaaI ACNGT 1 cut(s) 959
TaiI ACGT 1 cut(s) 1185
TaqI TCGA 2 cut(s) 573, 604
TatI WGTACW 3 cut(s) 285, 804, 1044
TfiI GAWTC 2 cut(s) 734, 743
Tru1I TTAA 6 cut(s) 461, 584, 705, 891, 1026, 1185
Tru9I TTAA 6 cut(s) 461, 584, 705, 891, 1026, 1185
TscAI CASTG 3 cut(s) 193, 565, 964
TseI GCWGC 1 cut(s) 506
TspDTI ATGAA 4 cut(s) 486, 539, 845, 892
TspRI CASTG 3 cut(s) 193, 565, 964
XapI RAATTY 3 cut(s) 474, 546, 798
XbaI TCTAGA 1 cut(s) 628
XceI RCATGY 2 cut(s) 292, 328
XspI CTAG 4 cut(s) 93, 629, 653, 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.