Rmu_sc0002542.1_g000008

Belongs to the PI3 PI4-kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002542.1
OG0007127
Physical Location & Seq
Reverse (-)
22704 .. 44738
22035 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 11697 bp
atgaggctgcagatggcaatggaggttcgggacagcctagagattgcgcacactgcggagtacttgaattttctaaaatgctacttccgggcgttctcggtgattcttctgcaaattacaacgccacagatgactgacaatccggaacacaagctccggaatatagtggtggaggttcttaatcgtctccctcacactgaagtgctccgccctttcgtccaggacctcctgaaagttgcaatgcaagtactaaccactgacaatgaggagaatggattgatatgtattcgtataatctttgacctcctcaggaacttcaggcccacgctggaaaatgaagttcagccatttttggactttgtctgcaaaatctacagcaattttgaggcaactgttaatcatttcttctttgtcaaagaagagaaaggtgcggcgcctgtggttggggaggatattaagtctatggatacttctttggatcagtcattaagtggtggtagtagttcttatactgcgggcgggaagcttaaccccagtacctgttccttcaagataattaccgagagtcccctggtggtcatgtttttgtttcagttgtatagtcgacttgtccagaccaatattcctaatctgttgcctaagatggttgctgctatatctgtccgtggtcccgaatctgttcctccgcacttgaagactcatttcactgagttgaagggtgcacaggtgaagactgtttcgtttctgacatacctgttgaagagctttgccgattatatcaggcagcatgaagaaagcatatgtaaaagcatagtcaatttgctagttacatgttcagactctgtatcaattagaaaggaattgttagtagctctaaaacatgttcttggaacagatttcaagaggggcttatttcctctgattgacacactattggaagaaagggttctggtcggaactggtcgagcctgctttgagacattgaggcctttggcatatagtctacttgcagagattgttcatcatgttcgagcggacctttccttgtcacagttatcgcggatcatatacttgttctcgagcaacatgcatgatgcttcattgtccttgagcattcatactacttgtgctcggttaatgctgaatctggtggaaccaatatttgagaaaggtgttgaccagccatctatggatgaagcacggattctattggggcgtatattggatgcttttgttgggaaattcagtactttcaagcgtactattcctcagctgttggaggaggctgaggagggaaaggatcgtgctactctaaggtccaagcttgagctccctgtgcaggcagtattgaatgtgcaggtcactgtggaacattccaaggaggtcaatgattgtaagcatctgataaagacattagtcatgggaatgaagaccatcatatggagtatcacacatgcgcatttacctcgctcacaggtttctccttctacgcatggaacgcacccacaggtgcttgtttccccttcatccagtttaccggcaccacaagcatttaaaggaatgcgagaagatgaggtccggaaggcttctggtgttttgaagagtggggtgcattgcttagcgctcttcaaggagaaggatgaagagagggaaatgatgcaactcttttcccagattttagctatcatggaacctagagatttaatggacatgttctcgctgtgtatgcccgagctttttgagtccatgattaacaacactcaattggttcacatattctctacgcttttgcaagcccctaaagtatacaagccttttgcagatgttttggttacttatcttgtgaacagtaaacttgatgtcttaaagtatccagatacacctgcagcaaaactggtcttgcatctctttcgcttcatttttggtgcagtttcaaaagcgcctcaagagtttgaacgtattttgcaacctcacgtgcctattataatggaagtctgcatgaaaaatgctactgaagttgaaaagcctcttggttacatgcaacttctccgcaccacctttcgagcgcttgcagtatgcaaatttgagcttcttctgagggatttgattcctatgttacaaccttgtcttaatatgctgttgatgatgcttgagggtccagctggggaagatatgaaggatcttttgctggaattgtgcttaaccttgcctgcacgcttgagctcgttgttaccgcaccttccccgtcttatgaagcctcttgttttatgtcttaaaggaagtgatgatcttgtgggcttaggtttaagaacacttgagttttgggtagacagtttaaatcctgatttcttggaacctagcatggctaatgttatgtctgaggtgattttagccctatggtctcacttgaggcctgccccctatccttggggagcgaaagcgttgcaactcctggggaagttgggtggtcgtaataggcgttttctcaaagagccccttgcccttgagtgcaaggagaatccagagcatggactccgtgtgattcttacatttgagcctgcaaccccatttttggtgccattggatcgatgcattaatcttgctgtggtagctgttatgaacaaaaatggtggaattgatacattttatcgaaagcaggctttgaagtttcttcgtgtttgcttatcatctcagcttaatttgccagaaaaggttactgatgatggatgcacacctagtcagttgtcaacattattagtttctgcagttgattcttcctggcagcggccagaaacttcagacataaaggctgacttaggcgtaaagacgaagacacaacttttggctgaaaagtctgttttcaagattctgttgatgactgttattgctgccagtgtggatcctgacttccatgaccccaaggatgacttcgttgtcaatgtctgccgccattttgcaatgatgtttcatattgactcatcctcaagtagcacttcagtttccactgctgcacttggtggtcctatgctttcttcaaatgtcaatattggttccagttccaggtcaaagaatagttcttcttcaaacctcaaagagttggatcctctaatatttttagatgctttagttgatgttctagcagatgaaaacagactccacgctaaagctgcacttagcgctttaaatgttttctgcgaaacacttctgtttcttgctcgctcgaagcatgctgatgtgctgatgtcaagaggccctggtactccaatgatggtctctagtccatcattaaatcctgtatactctccacctccaagtgttcgcatccctgtttttgagcaacttctgccccgtctattgcattgttgctatggaacaacctggcaagcacagatgggaggtgttatgggacttggtgctttggttgggaaggtcactgttgagactttgtgccttttccaagtaagaattgttcgcggtttggtatatgttcttaaaaggcttcctacttatgctagcaaggagcaagaagagaccagtcaggtgcttacgcaggttcttcgtgtagttaataatgttgacgaagcaaatagtgaacctcgccggcaaagttttcaaggagtagttgatttcctttcttcagagttgtttaatcccaatgcatctgttattgtgaggaagaatgtgcagtcatgtttggcacttttggctagtagaactggtagtgaggtatctgagttgcttgagcctttgtatcaacctttgcttcagcctcttttggtgcgaccgctccgatcaaaaactgtagatcaacaggtgggaacagttactgctttaaacttctgcttggccttgaggccacctcttctcaagcttactcaggatttggttaactttctgcaagaagcattgcaaatagctgaggctgatgaaactgtatgggttgtgaagtttatgaacccaaaagtggccacgtcactaaataagcttcgaacagcttgcattgagttactctgtactacaatggcatgggcagatttcaaaactcaaaatcatgctgaattgcgtgcaaagatcatttccatgtttttcaaatctttgacttgtcgaacccctgaaatcgttgctgtggctaaggaaggcttacgacaggttataaatcagcagaggatgccaaaagagcttcttcagagcagccttaggcctattttggtcaacttggcacatacaaagaatctgagcatgcctcttctgcaaggtctggcccggcttctagagcttctgtctaattggtttaatgttaccctcggtggcaagttgctagaacacctaaagaaatggttagaacctgaaaaacttgcacaaagtcagaaatcatggaaggccggtgaagaaccaaaaattgcagctgctattattgagctttttcaccttcttcctatggctgcttcaaaattccttgatgaacttgtaactttgaccattgaattggaaggagctcttctccccggacaagtatacagcgagattaatagtccgtatcgtcttccacttacaaagtttttgaatcgctatgcaagtcttgctatcgattattttcttgcgcgattaagtgaaccaaagtacttcaggaggtttatgtatataatccgctcggatgccggccagccattgagagatgaacttgctaaatcgccacagaaaatacttgcaaatgcctttcctgagtttttgccaaactatgatgtagggatggcttcggggtcatcaactccacctccagctttgttgggtgacgaaggtcttgctaagcctccacctgatagttcgaatccaccatctgcacaagctggtgcaacttcagatgcttattttcggggacttgcacttgttaaaactttagtcaaattgatacctggctggctacagagtaatcgcaatgtgtttgaaaccttggttcttgtttggaagtcgcatgcaagactatctcggttgcagaatgaacaggagctggacttagtgcaggttaaagaaagcaaatggcttgtcaaatgcttcttgaattatttacgccacgagaaaacagaagtgaatgttctctttgatatactcaccatcttcttgtttcattctcgaattgattatacatttctgaaagagtactatatcattgaggttgcagaaggttatccaccgaactttaaaaaggcacttctcttgcattttttgagtcttttccaatcgaaacaacttggtcatgatcacttggtggttattatgcaaatgcttattcttccaatgcttgctcatgccttccaaaatgatcaaagttgggaagttgtagaccaggctattgtgaaaacaattgttgacaaacttcttgatcctccagaagaggtttctgctgaatacgacgagccgttgagaatagaacttttgcagcttgccactttgctgctcaagtatctgcagaatgaccttgtccatcataggaaagaactgatcaaatttggttggaatcatctcaaacgtgaagatagtgccagtaaacagtgggcatttgtaaatgtttgtcacttcttggaggcctatcaagccccagaaaaaatcatactacaggtttttgtggcactgctgagaacttgccagccagagaataaaatgctggtcaaacaggccctcgatatactaatgccagcactgccccgaaggttgcctcttggagatattcgtatgccaatttggatacgagctcaatttgtacctcagatggtcaattctctcagtcgccttggattgccttacaacaccactgccgagaacaggcgtcttgcaattgaacttgctggtttggtggttggctgggaaagacaacgacaaaatgaaatgaaagtggtagctgatagtgatggcaccaatcagatcactgaatttaatcccggcccttctagtgctgatattaagcgctctgtggatgggtctacatttcctgaagattcaacgaaacgtgtcaaagttgaacctggtcttcaatccctctgtgtaatgtcacctggtggtgcatcatcaattcctaacattgagaccccaggatctgctagccaacctgatgaagaattcaagccaaatgctgctatggaagaaatgataatcaatttccttataagggttgcattggtcatagagcctaaggacaaggaagcaagtaccatgtacaaacaagctctggagctactttcacaagctttggaggtgtggccaactgccaatgttaaattcaattatcttgagaagctgctgagcagcattcagcctccttccaaggacccttccactgccctggcacaggggttggatgtgatgaacaaggttctagagaagcagccgcatctcttcatcagaaacaaccttaaccagatttcccagattcttgaaccgtgtttcaagttgaaactgttagatgctggaaagtcattgtgctccatgttgaaaatggtctttgttgcttttcctctggaagcagctaccactccccctgatgtgaaactactatatcagaaggttgatgaactaatacaaaagcacatgaatactgttccaactcctcaaacacctggtgaagagagcaatgttaattcagttagttttgtcctgcttgtgattagaaccttgacagaggtgcagagtaacttcattgatcctaccatcttggttcgtattcttcagcgcctggcaagagaaatgggaccatcatctggttctcatgtgagacaaggccagaaagatttggattctgcggtctcttcttctcgtcaaggtgctgatgctggagcagtgatttctaatctgaaatctgtgctgaaacttattaatgaaagggtcatgcttgtttctgagtgcaaaagatcggttactcaaatattaaatagtctgctatctgagaaaggtactgattctagtgtgcttctatccatactagatgtaataaaagggtggattgaagatgacttcggaaagccaggcacatcagtctcatcaagtgcttttctcactccaaaagaaatagtttcctttcttcagaagctttcactagttgataagcaaaacttctcagatgctctggacgagtgggacagtaaatatcttgagcttctatatggattatgtgccgattcaaataaatatcccctatcattgcgtcaagaggtgtttctgaaggtggaaaggcaattcatgcttggtctacgagcaagagatcctgaatctaggatgaaatttttctcaatttaccatgaatctcttgggaaatcattgtttgctaggcttcagtacattattcaccttcaggactgggaagctttgagtgatgtcttctggctcaaacagggccttgatcttcttttagcaatcctagtcgaggataaggccattacacttgctccaaattcagcgaaggtggcaccactgctgatttcaggttctccagatgcttctgggatgcagcaccaggttactgatgttccagagggttcagaagatgctccattaacatttgatattcttgttcgcaagcatgcacagtttttgaatgagatgagtaaacttaaggtggctgatctcattattccactgagagagcttgctcatatggatgcaaatgttgcatatcatttgtgggtgttggtgttccccattgtctgggtaaccttacacaaggaagagcaggtagcattagctaaaccaatgattactctcctctctaaagattatcataaaaagcagcaaggcaatagaccaaatgttgtgcaagcacttctggaggggcttcagttgagccatcctcaacctcggatgccaagtgagctcattaaatatattgggaaaacttacaatgcatggcatatagcattagcactgctggagagtcatgtcatgttgttcacaaatgatacaaagtgctccgagtcgcttgctgaactttatcgtttgctcaatgaagaagatatgaggtgtgggttgtggaagaaaagatcaatcactgcagagactagagccgggctttctctagttcagcatggttattggcagcgtgctcaaagcctcttttaccaagcaatggttaaggcaactcaaggcacatataacaacactgtaccaaaagctgagatgtgtctctgggaagaacaatggctgtactgtgctggtcaacttagccaatgggatgcattggtagactttggcaagagtattgagaattacgaaatactactcgacagtctttggaaattgcctgattgggcatttatgaaggatgttgtaattccaaaggctcaggtggaagaaactccaaaacttcggcttgttcaagcattttttgctctccatgataaaaatgcaaatggtgttggggatgctgagaacgttgtggggaaaggtgttgatcttgctttagaacagtggtggcagttgccacaaatgtctgttcattccaggattccgttgttgcagcaattccaacagctggttgaagttcaagaatcatcaagaatacttgtggatattgccaacggaaacaaacttgctgcgaactctgtggttggtgtgcatggaaatctttacgctgacctcaaagacatccttgaaacgtggagactcagaactcctaatgattgggataacatgtcggtttggtatgatttgctacaatggagaaatgaaatgtataatgcagtcatagatgcattcaaagattttacaactacgaatcctcaacttcatcaccttggttatcgtgacaaagcatggaatgtcaataaacttgctcgcattggtcgtaagcagggcctttatgatgtttgtgtgacaatacttgaaaagatgtatggccattcaaccatggaagtgcaggaggcctttgttaagataagagaacaagcaaaagcttatctggagatgaagggagagcttaccagtggtctcaatctaattaacagcactaacctagagtattttccagtgaaacataaggcagaaatatttcgtcttaagggggatttcttgttaaagctgaatgactctgaaggtgctaatcatgcatactctaatgccatcagcttgttcaagaatttgcctaaaggatggataagctggggaaattattgtgacatggcttacagagaaatgcatgaagagatttggctggaatatgctgtgagctgctttcttcaaggcatcaaatttggcatatctaactcaagaagccatctggctcgtgttctatatcttctcagctttgatactcctaatgaacctgttgggagggcttttgataaatatcttgaccaaattccccactgggtctggctttcttggattccacaactcttactttctttgcaaaggacagaagctccccattgcaaactggtcctacttaaaatcgcaaccgtgtatccccaggccctatattattggcttcgcacatatttgcttgaacggcgtgatgttgcaaataaaactgaactcggtagtaggatggcaatggctcaaagaatgcagcaaagtgccactggtgccactgctggttcaattggcttagctgatgggaatgctagagtacaaggtcatagtggtttatcctcggacaatcaagttcaccaagctgctcagtctggtgggggtattggttctcatgacggtggaaactctcacgggcaagaatctgaaaggtctacaggtgttgagagcagcatgcatccagggaatgagcaacaaggtgcgtcaaccatcaatgatggtggtcagaatgcattaaggcgtaatggtgctttcggttccctaccttctgctgccagtgcttttgatgctgccaaggatataatggaggctcttagaagcaaacatactaatttggctactgaacttgagagtctgctcactgaaattggttcgaggtttgtcactttgcccgaagagagacttctagcggtggttaatgcactgctccaccgttgttacaagtatccaacggcgaccacagctgaggttccccaatctcttaagaaggagctttctggtgtttgccgagcttgcttttcagcagatgctgtaaataagcacgttgattttgtgagagagtacaaacaagatttcgagcgtgatcttgatcctgggagcactcctacttttccgtcgaccctctctgagttgactgaacgtttaaaacattggaaaaatgtcctccagagcaatgttgaagacaggtttccagcggttttgaagctggaagaggaaagcagggtgttgcgtgactttcatgttgtggatgttgaagttccggggcagtacttcagtgatcaggaaattgcacccgaccataccgtaaagttggatagggttggagctgatatacctattgtacgaaggcatggaagcagtttccggcgcttgactcttattggttctgatggatctcagcgtcattttattgtccagacatctttgactcctaatgcaagaagtgatgaacgaatattgcagcttttccgtgtgatgaaccagatgtttgataagcacaaggaatcaaggcgacgccacatatgcattcacactccaatcattatacctgtttggtcacaggtccgtatggtagaagatgatttgatgtacagcaccttccttgaggtgtatgagaaccattgtgcaaggaatgacaaggaggcagatcttcccatcacctatttcaaggagcagttaaaccaagctatatctggccaaatctcaccagaagctgttatagaccttcgcctccaagcttataatgacatcacaagaaatcttgtaacggacgggatcttttcccagtacatgtacaagactctaccaagtggcaaccatatgtgggctttcaaaaagcagtttgccatccagttagccctctcgagtttcatgtccctcatgttacaaattggggggaggtcgccaaacaaaatattatttgccaagaacacaggaaaaatatttcaaactgattttcatcctgcatatgatgcaaatggaatgatcgaatttaatgaaccagtgcctttccgtcttacgaggaatatgcaatctttcttctcccattttggagtggaaggcctcattgtgtctgcaatgtgtgcagcagcacaggctgttgtttcacctaaacaaagtcagcatctatggcatcagttggctatgttcttccgcgatgagcttctgtcttggtcttggaggagaccacttgggatgactatggcccccttttccggagggggtagtatgaacccagctgatttcaagcagaaggtcatcaacaatgttgagcacgtgattaaccggatcaatggaattgccccacagtacttctctgaagaggaggagaatgccatggaaccaccacaatcggttcagcgtggagtgactgagttagtggaagcagccttgactccgaggaacttgtgcatgatggatccaacgtggcacgcctggacagagatcttgctcggacccgtaaacaatggcagtcttggcagtgacgacaacgacgatgtgagaagagaacactcgtcttggcgtactcgagccagagaactaaatcggtgtattccagtgcgattcggatcttggccgggtcctgcttcaattcccctcgacccttccttgatcatgatttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04141 ko04210 ko04932 ko05010 ko05166 map04140 map04141 map04210 map04932 map05010 map05166
Pfam Domains
Protein Families
FAT FATC PI3_PI4_kinase

Protein Analysis

3898

Amino Acids

438.98

Weight (kDa)

6.64

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tra1_central PF20175 235 - 806 1.6e-151 Tra1 HEAT repeat central region
Tra1_ring PF20206 817 - 1916 7.3e-277 Tra1 HEAT repeat ring region
Tra1_ring PF20206 1916 - 2609 6.5e-131 Tra1 HEAT repeat ring region
FAT PF02259 2754 - 3092 1e-46 FAT domain
PI3_PI4_kinase PF00454 3504 - 3750 4.8e-27 Phosphatidylinositol 3- and 4-kinase
FATC PF02260 3807 - 3837 8.4e-06 FATC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1985, 4154, 6161, 10815
AarI CACCTGC 1 cut(s) 1891
AasI GACNNNNNNGTC 1 cut(s) 2933
Acc16I TGCGCA 2 cut(s) 48, 1458
Acc36I ACCTGC 5 cut(s) 1348, 1891, 3541, 5011, 7711
AccB1I GGYRCC 6 cut(s) 433, 1540, 2575, 5909, 7456, 9569
AccB7I CCANNNNNTGG 6 cut(s) 1440, 2528, 6752, 7695, 8113, 8518
AccBSI CCGCTC 3 cut(s) 1034, 3787, 4650
AccII CGCG 4 cut(s) 1060, 3474, 4603, 11229
AccIII TCCGGA 4 cut(s) 142, 156, 1578, 11288
AclI AACGTT 2 cut(s) 8418, 10213
AcoI YGGCCR 7 cut(s) 2786, 3966, 4660, 6254, 8881, 10768, 11648
AcvI CACGTG 2 cut(s) 1975, 11350
AcyI GRCGYC 3 cut(s) 434, 5824, 10588
AdeI CACNNNGTG 4 cut(s) 3017, 5266, 6053, 6614
AfeI AGCGCT 4 cut(s) 1623, 2067, 3178, 5963
AflII CTTAAG 3 cut(s) 7601, 9041, 10055
AflIII ACRYGT 6 cut(s) 830, 880, 1710, 6004, 8676, 10863
AhlI ACTAGT 1 cut(s) 7087
AjiI CACGTC 1 cut(s) 3972
AjuI GAANNNNNNNTTGG 6 cut(s) 2013, 2045, 2912, 2944, 6257, 6289
AleI CACNNNNGTG 1 cut(s) 200
AloI GAACNNNNNNTCC 2 cut(s) 138, 170
Alw44I GTGCAC 1 cut(s) 720
AlwNI CAGNNNCTG 8 cut(s) 540, 842, 3827, 5008, 6092, 6727, 10103, 10787
Ama87I CYCGRG 4 cut(s) 1078, 1730, 10936, 11601
Aor13HI TCCGGA 4 cut(s) 142, 156, 1578, 11288
Aor51HI AGCGCT 4 cut(s) 1623, 2067, 3178, 5963
ApaLI GTGCAC 1 cut(s) 720
ArsI GACNNNNNNTTYG 4 cut(s) 2824, 2856, 5028, 5060
AseI ATTAAT 3 cut(s) 2595, 4527, 6867
Asp700I GAANNNNTTC 5 cut(s) 678, 945, 3201, 7274, 9033
AsuC2I CCSGG 7 cut(s) 89, 4264, 4506, 5937, 8053, 10335, 11652
AsuII TTCGAA 2 cut(s) 3988, 4826
AsuNHI GCTAGC 2 cut(s) 3512, 6095
AvaI CYCGRG 4 cut(s) 1078, 1730, 10936, 11601
AxyI CCTNAGG 3 cut(s) 308, 4196, 6186
BaeGI GKGCMC 1 cut(s) 724
BaeI ACNNNNGTAYC 2 cut(s) 520, 553
BalI TGGCCA 4 cut(s) 3968, 6256, 8883, 10770
BamHI GGATCC 3 cut(s) 2899, 3100, 11491
BanI GGYRCC 6 cut(s) 433, 1540, 2575, 5909, 7456, 9569
BanII GRGCYC 6 cut(s) 1332, 2225, 2496, 4498, 5752, 7863
BarI GAAGNNNNNNTAC 4 cut(s) 9883, 9915, 10655, 10687
BauI CACGAG 2 cut(s) 5072, 9267
BbrPI CACGTG 2 cut(s) 1975, 11350
BbsI GAAGAC 8 cut(s) 701, 737, 1436, 2837, 4535, 6017, 7360, 10260
BbvCI CCTCAGC 4 cut(s) 1269, 1287, 3918, 10038
BceAI ACGGC 3 cut(s) 5401, 9509, 10041
BciVI GTATCC 5 cut(s) 460, 1881, 5736, 9459, 10029
BclI TGATCA 5 cut(s) 5257, 5320, 5499, 10351, 11685
BcnI CCSGG 7 cut(s) 89, 4264, 4506, 5937, 8053, 10335, 11652
BcuI ACTAGT 1 cut(s) 7087
BfoI RGCGCY 8 cut(s) 437, 1625, 1943, 2069, 3180, 5965, 6727, 10444
BfrI CTTAAG 3 cut(s) 7601, 9041, 10055
BfuAI ACCTGC 5 cut(s) 1348, 1891, 3541, 5011, 7711
BfuI GTATCC 5 cut(s) 460, 1881, 5736, 9459, 10029
BglII AGATCT 2 cut(s) 10720, 11517
BlpI GCTNAGC 4 cut(s) 1618, 4806, 6296, 9592
BmcAI AGTACT 7 cut(s) 62, 249, 1249, 4622, 5159, 10343, 11384
BmeT110I CYCGRG 4 cut(s) 1078, 1730, 10936, 11601
BmgBI CACGTC 1 cut(s) 3972
BmrI ACTGGG 4 cut(s) 528, 7357, 9361, 10852
BmtI GCTAGC 2 cut(s) 3516, 6099
BmuI ACTGGG 4 cut(s) 528, 7357, 9361, 10852
BoxI GACNNNNGTC 2 cut(s) 1415, 4797
BpiI GAAGAC 8 cut(s) 701, 737, 1436, 2837, 4535, 6017, 7360, 10260
BplI GAGNNNNNCTC 8 cut(s) 3844, 3876, 4228, 4260, 4485, 4517, 7822, 7854
BpmI CTGGAG 9 cut(s) 4761, 5370, 6245, 6846, 7464, 7835, 7937, 8966, 10223
Bpu10I CCTNAGC 7 cut(s) 1269, 1287, 2299, 3918, 4131, 8328, 10038
Bpu1102I GCTNAGC 4 cut(s) 1618, 4806, 6296, 9592
Bpu14I TTCGAA 2 cut(s) 3988, 4826
BpuMI CCSGG 7 cut(s) 89, 4264, 4506, 5937, 8053, 10335, 11652
Bsa29I ATCGAT 2 cut(s) 2587, 4587
BsaAI YACGTR 2 cut(s) 1975, 11350
BsaBI GATNNNNATC 4 cut(s) 9330, 10161, 11031, 11641
BsaHI GRCGYC 3 cut(s) 434, 5824, 10588
BsaI GGTCTC 7 cut(s) 2406, 3277, 3524, 6074, 6802, 8978, 11251
BsaWI WCCGGW 5 cut(s) 142, 156, 1578, 11288, 11358
BsaXI ACNNNNNCTCC 8 cut(s) 138, 168, 4759, 4789, 7420, 7450, 9391, 9421
Bse118I RCCGGY 4 cut(s) 1537, 3600, 4382, 4658
Bse21I CCTNAGG 3 cut(s) 308, 4196, 6186
Bse8I GATNNNNATC 4 cut(s) 9330, 10161, 11031, 11641
BseAI TCCGGA 4 cut(s) 142, 156, 1578, 11288
BseCI ATCGAT 2 cut(s) 2587, 4587
BseJI GATNNNNATC 4 cut(s) 9330, 10161, 11031, 11641
BseRI GAGGAG 9 cut(s) 281, 296, 1295, 1304, 6591, 7742, 11269, 11411, 11414
BseSI GKGCMC 1 cut(s) 724
BseYI CCCAGC 4 cut(s) 2162, 5859, 9144, 11308
Bsh1236I CGCG 4 cut(s) 1060, 3474, 4603, 11229
Bsh1285I CGRYCG 1 cut(s) 3785
BshNI GGYRCC 6 cut(s) 433, 1540, 2575, 5909, 7456, 9569
BshVI ATCGAT 2 cut(s) 2587, 4587
BsiEI CGRYCG 1 cut(s) 3785
BsiHKCI CYCGRG 4 cut(s) 1078, 1730, 10936, 11601
BslFI GGGAC 8 cut(s) 44, 552, 654, 3420, 4890, 6756, 7142, 10933
BsmBI CGTCTC 1 cut(s) 191
BsmFI GGGAC 8 cut(s) 44, 552, 654, 3420, 4890, 6756, 7142, 10933
BsmI GAATGC 9 cut(s) 1113, 1566, 6303, 8738, 9555, 9610, 9808, 10599, 11410
Bso31I GGTCTC 7 cut(s) 2406, 3277, 3524, 6074, 6802, 8978, 11251
BsoBI CYCGRG 4 cut(s) 1078, 1730, 10936, 11601
Bsp119I TTCGAA 2 cut(s) 3988, 4826
Bsp13I TCCGGA 4 cut(s) 142, 156, 1578, 11288
Bsp1407I TGTACA 3 cut(s) 6210, 10662, 10866
Bsp1720I GCTNAGC 4 cut(s) 1618, 4806, 6296, 9592
Bsp19I CCATGG 2 cut(s) 8892, 11409
BspDI ATCGAT 2 cut(s) 2587, 4587
BspEI TCCGGA 4 cut(s) 142, 156, 1578, 11288
BspFNI CGCG 4 cut(s) 1060, 3474, 4603, 11229
BspHI TCATGA 3 cut(s) 5254, 9688, 11688
BspMAI CTGCAG 5 cut(s) 12, 1888, 2767, 5469, 8041
BspMI ACCTGC 5 cut(s) 1348, 1891, 3541, 5011, 7711
BspOI GCTAGC 2 cut(s) 3516, 6099
BspQI GCTCTTC 4 cut(s) 755, 1631, 4503, 7710
BspT104I TTCGAA 2 cut(s) 3988, 4826
BspT107I GGYRCC 6 cut(s) 433, 1540, 2575, 5909, 7456, 9569
BspTI CTTAAG 3 cut(s) 7601, 9041, 10055
BspTNI GGTCTC 7 cut(s) 2406, 3277, 3524, 6074, 6802, 8978, 11251
BsrBI CCGCTC 3 cut(s) 1034, 3787, 4650
BsrFI RCCGGY 4 cut(s) 1537, 3600, 4382, 4658
BsrGI TGTACA 3 cut(s) 6210, 10662, 10866
BssAI RCCGGY 4 cut(s) 1537, 3600, 4382, 4658
BssNAI GTATAC 3 cut(s) 1807, 3298, 4516
BssNI GRCGYC 3 cut(s) 434, 5824, 10588
BssSI CACGAG 2 cut(s) 5072, 9267
Bst1107I GTATAC 3 cut(s) 1807, 3298, 4516
Bst2BI CACGAG 2 cut(s) 5072, 9267
BstACI GRCGYC 3 cut(s) 434, 5824, 10588
BstAFI CTTAAG 3 cut(s) 7601, 9041, 10055
BstAPI GCANNNNNTGC 8 cut(s) 3343, 4168, 4580, 7231, 7658, 8372, 11045, 11156
BstAUI TGTACA 3 cut(s) 6210, 10662, 10866
BstBAI YACGTR 2 cut(s) 1975, 11350
BstBI TTCGAA 2 cut(s) 3988, 4826
BstDSI CCRYGG 3 cut(s) 664, 8892, 11409
BstEII GGTNACC 1 cut(s) 7699
BstENI CCTNNNNNAGG 2 cut(s) 6341, 7412
BstFNI CGCG 4 cut(s) 1060, 3474, 4603, 11229
BstH2I RGCGCY 8 cut(s) 437, 1625, 1943, 2069, 3180, 5965, 6727, 10444
BstMCI CGRYCG 1 cut(s) 3785
BstPAI GACNNNNGTC 2 cut(s) 1415, 4797
BstPI GGTNACC 1 cut(s) 7699
BstSLI GKGCMC 1 cut(s) 724
BstUI CGCG 4 cut(s) 1060, 3474, 4603, 11229
BstV2I GAAGAC 8 cut(s) 701, 737, 1436, 2837, 4535, 6017, 7360, 10260
BstXI CCANNNNNNTGG 2 cut(s) 4486, 6337
BstZ17I GTATAC 3 cut(s) 1807, 3298, 4516
Bsu15I ATCGAT 2 cut(s) 2587, 4587
Bsu36I CCTNAGG 3 cut(s) 308, 4196, 6186
BsuI GTATCC 5 cut(s) 460, 1881, 5736, 9459, 10029
BsuTUI ATCGAT 2 cut(s) 2587, 4587
BtgI CCRYGG 3 cut(s) 664, 8892, 11409
BtgZI GCGATG 1 cut(s) 11244
BtrI CACGTC 1 cut(s) 3972
BveI ACCTGC 5 cut(s) 1348, 1891, 3541, 5011, 7711
CaiI CAGNNNCTG 8 cut(s) 540, 842, 3827, 5008, 6092, 6727, 10103, 10787
CciI TCATGA 3 cut(s) 5254, 9688, 11688
Cfr10I RCCGGY 4 cut(s) 1537, 3600, 4382, 4658
ClaI ATCGAT 2 cut(s) 2587, 4587
CseI GACGC 5 cut(s) 5813, 7184, 9765, 10463, 10596
CsiI ACCWGGT 4 cut(s) 6019, 6049, 6610, 7503
CspCI CAANNNNNGTGG 4 cut(s) 2611, 2646, 9775, 9810
DinI GGCGCC 1 cut(s) 435
DraI TTTAAA 6 cut(s) 1555, 2337, 3183, 3834, 5200, 10218
DraIII CACNNNGTG 4 cut(s) 3017, 5266, 6053, 6614
DrdI GACNNNNNNGTC 1 cut(s) 2933
DseDI GACNNNNNNGTC 1 cut(s) 2933
EaeI YGGCCR 7 cut(s) 2786, 3966, 4660, 6254, 8881, 10768, 11648
EciI GGCGGA 1 cut(s) 197
Ecl136II GAGCTC 5 cut(s) 1330, 2223, 4496, 5750, 7861
Eco147I AGGCCT 6 cut(s) 988, 2413, 4201, 5585, 8909, 11136
Eco24I GRGCYC 6 cut(s) 1332, 2225, 2496, 4498, 5752, 7863
Eco31I GGTCTC 7 cut(s) 2406, 3277, 3524, 6074, 6802, 8978, 11251
Eco47III AGCGCT 4 cut(s) 1623, 2067, 3178, 5963
Eco53kI GAGCTC 5 cut(s) 1330, 2223, 4496, 5750, 7861
Eco72I CACGTG 2 cut(s) 1975, 11350
Eco81I CCTNAGG 3 cut(s) 308, 4196, 6186
Eco88I CYCGRG 4 cut(s) 1078, 1730, 10936, 11601
Eco91I GGTNACC 1 cut(s) 7699
EcoICRI GAGCTC 5 cut(s) 1330, 2223, 4496, 5750, 7861
EcoNI CCTNNNNNAGG 2 cut(s) 6341, 7412
EcoO109I RGGNCCY 8 cut(s) 223, 3251, 5674, 6322, 7384, 8839, 9457, 11654
EcoO65I GGTNACC 1 cut(s) 7699
EcoRI GAATTC 1 cut(s) 6113
EcoT38I GRGCYC 6 cut(s) 1332, 2225, 2496, 4498, 5752, 7863
EgeI GGCGCC 1 cut(s) 435
EheI GGCGCC 1 cut(s) 435
Esp3I CGTCTC 1 cut(s) 191
FaqI GGGAC 8 cut(s) 44, 552, 654, 3420, 4890, 6756, 7142, 10933
FauI CCCGC 2 cut(s) 508, 512
FauNDI CATATG 6 cut(s) 800, 1439, 7644, 10595, 10893, 11041
FbaI TGATCA 5 cut(s) 5257, 5320, 5499, 10351, 11685
FriOI GRGCYC 6 cut(s) 1332, 2225, 2496, 4498, 5752, 7863
FspAI RTGCGCAY 1 cut(s) 1458
FspI TGCGCA 2 cut(s) 48, 1458
GsaI CCCAGC 4 cut(s) 2166, 5863, 9148, 11312
GsuI CTGGAG 9 cut(s) 4761, 5370, 6245, 6846, 7464, 7835, 7937, 8966, 10223
HaeII RGCGCY 8 cut(s) 437, 1625, 1943, 2069, 3180, 5965, 6727, 10444
HgaI GACGC 5 cut(s) 5813, 7184, 9765, 10463, 10596
Hin1I GRCGYC 3 cut(s) 434, 5824, 10588
HindIII AAGCTT 9 cut(s) 524, 1322, 3869, 3983, 6240, 7079, 7353, 8937, 10809
HpaI GTTAAC 1 cut(s) 3889
Hpy99I CGWCG 4 cut(s) 5414, 10192, 10590, 11570
Hsp92I GRCGYC 3 cut(s) 434, 5824, 10588
KasI GGCGCC 1 cut(s) 433
Kpn2I TCCGGA 4 cut(s) 142, 156, 1578, 11288
KroI GCCGGC 2 cut(s) 3600, 4658
KroNI GCCGGC 2 cut(s) 3602, 4660
Ksp22I TGATCA 5 cut(s) 5257, 5320, 5499, 10351, 11685
KspAI GTTAAC 1 cut(s) 3889
LguI GCTCTTC 4 cut(s) 755, 1631, 4503, 7710
MabI ACCWGGT 4 cut(s) 6019, 6049, 6610, 7503
MbiI CCGCTC 3 cut(s) 1034, 3787, 4650
MfeI CAATTG 4 cut(s) 1763, 5361, 5832, 9585
MlsI TGGCCA 4 cut(s) 3968, 6256, 8883, 10770
MluNI TGGCCA 4 cut(s) 3968, 6256, 8883, 10770
Mly113I GGCGCC 1 cut(s) 434
Mox20I TGGCCA 4 cut(s) 3968, 6256, 8883, 10770
MroI TCCGGA 4 cut(s) 142, 156, 1578, 11288
MroNI GCCGGC 2 cut(s) 3600, 4658
MroXI GAANNNNTTC 5 cut(s) 678, 945, 3201, 7274, 9033
MscI TGGCCA 4 cut(s) 3968, 6256, 8883, 10770
MslI CAYNNNNRTG 7 cut(s) 200, 1424, 3231, 4079, 7647, 8897, 9693
Msp20I TGGCCA 4 cut(s) 3968, 6256, 8883, 10770
MspA1I CMGCKG 8 cut(s) 1273, 2162, 2785, 4406, 8518, 10037, 10268, 11312
MspCI CTTAAG 3 cut(s) 7601, 9041, 10055
MunI CAATTG 4 cut(s) 1763, 5361, 5832, 9585
Mva1269I GAATGC 9 cut(s) 1113, 1566, 6303, 8738, 9555, 9610, 9808, 10599, 11410
MvnI CGCG 4 cut(s) 1060, 3474, 4603, 11229
NaeI GCCGGC 2 cut(s) 3602, 4660
NarI GGCGCC 1 cut(s) 434
NciI CCSGG 7 cut(s) 89, 4264, 4506, 5937, 8053, 10335, 11652
NcoI CCATGG 2 cut(s) 8892, 11409
NdeI CATATG 6 cut(s) 800, 1439, 7644, 10595, 10893, 11041
NgoMIV GCCGGC 2 cut(s) 3600, 4658
NheI GCTAGC 2 cut(s) 3512, 6095
NmeAIII GCCGAG 2 cut(s) 5839, 10106
NsbI TGCGCA 2 cut(s) 48, 1458
NspV TTCGAA 2 cut(s) 3988, 4826
OliI CACNNNNGTG 1 cut(s) 200
PaeI GCATGC 5 cut(s) 3230, 4243, 4976, 7574, 9751
PaeR7I CTCGAG 3 cut(s) 1078, 10936, 11601
PagI TCATGA 3 cut(s) 5254, 9688, 11688
PaqCI CACCTGC 1 cut(s) 1891
PceI AGGCCT 6 cut(s) 988, 2413, 4201, 5585, 8909, 11136
PciI ACATGT 5 cut(s) 830, 880, 1710, 8676, 10863
PciSI GCTCTTC 4 cut(s) 755, 1631, 4503, 7710
PcsI WCGNNNNNNNCGW 3 cut(s) 2476, 8827, 11565
PctI GAATGC 9 cut(s) 1113, 1566, 6303, 8738, 9555, 9610, 9808, 10599, 11410
PdiI GCCGGC 2 cut(s) 3602, 4660
PdmI GAANNNNTTC 5 cut(s) 678, 945, 3201, 7274, 9033
PflFI GACNNNGTC 2 cut(s) 359, 5477
PflMI CCANNNNNTGG 6 cut(s) 1440, 2528, 6752, 7695, 8113, 8518
PfoI TCCNGGA 2 cut(s) 219, 8486
PluTI GGCGCC 1 cut(s) 437
PmaCI CACGTG 2 cut(s) 1975, 11350
PmlI CACGTG 2 cut(s) 1975, 11350
Ppu21I YACGTR 2 cut(s) 1975, 11350
PpuMI RGGWCCY 3 cut(s) 223, 6322, 11654
PscI ACATGT 5 cut(s) 830, 880, 1710, 8676, 10863
PshAI GACNNNNGTC 2 cut(s) 1415, 4797
PshBI ATTAAT 3 cut(s) 2595, 4527, 6867
PsiI TTATAA 4 cut(s) 1985, 4154, 6161, 10815
Psp124BI GAGCTC 5 cut(s) 1332, 2225, 4498, 5752, 7863
Psp1406I AACGTT 2 cut(s) 8418, 10213
Psp5II RGGWCCY 3 cut(s) 223, 6322, 11654
PspCI CACGTG 2 cut(s) 1975, 11350
PspEI GGTNACC 1 cut(s) 7699
PspFI CCCAGC 4 cut(s) 2162, 5859, 9144, 11308
PspPPI RGGWCCY 3 cut(s) 223, 6322, 11654
PspXI VCTCGAGB 1 cut(s) 11601
PsrI GAACNNNNNNTAC 2 cut(s) 3453, 3485
PstI CTGCAG 5 cut(s) 12, 1888, 2767, 5469, 8041
PstNI CAGNNNCTG 8 cut(s) 540, 842, 3827, 5008, 6092, 6727, 10103, 10787
PsyI GACNNNGTC 2 cut(s) 359, 5477
PvuII CAGCTG 6 cut(s) 1273, 2162, 4406, 8518, 10037, 11312
RseI CAYNNNNRTG 7 cut(s) 200, 1424, 3231, 4079, 7647, 8897, 9693
SacI GAGCTC 5 cut(s) 1332, 2225, 4498, 5752, 7863
SalI GTCGAC 2 cut(s) 603, 10189
SapI GCTCTTC 4 cut(s) 755, 1631, 4503, 7710
ScaI AGTACT 7 cut(s) 62, 249, 1249, 4622, 5159, 10343, 11384
SexAI ACCWGGT 4 cut(s) 6019, 6049, 6610, 7503
SfoI GGCGCC 1 cut(s) 435
Sfr274I CTCGAG 3 cut(s) 1078, 10936, 11601
SfuI TTCGAA 2 cut(s) 3988, 4826
SlaI CTCGAG 3 cut(s) 1078, 10936, 11601
SmiMI CAYNNNNRTG 7 cut(s) 200, 1424, 3231, 4079, 7647, 8897, 9693
SpeI ACTAGT 1 cut(s) 7087
SphI GCATGC 5 cut(s) 3230, 4243, 4976, 7574, 9751
SseBI AGGCCT 6 cut(s) 988, 2413, 4201, 5585, 8909, 11136
SspDI GGCGCC 1 cut(s) 433
SspI AATATT 9 cut(s) 622, 1161, 3046, 3111, 6918, 9033, 10530, 10989, 11016
SstI GAGCTC 5 cut(s) 1332, 2225, 4498, 5752, 7863
StuI AGGCCT 6 cut(s) 988, 2413, 4201, 5585, 8909, 11136
TauI GCSGC 4 cut(s) 434, 2788, 2949, 6385
Tth111I GACNNNGTC 2 cut(s) 359, 5477
Van91I CCANNNNNTGG 6 cut(s) 1440, 2528, 6752, 7695, 8113, 8518
Vha464I CTTAAG 3 cut(s) 7601, 9041, 10055
VneI GTGCAC 1 cut(s) 720
VspI ATTAAT 3 cut(s) 2595, 4527, 6867
XagI CCTNNNNNAGG 2 cut(s) 6341, 7412
XbaI TCTAGA 2 cut(s) 4270, 6370
XcmI CCANNNNNNNNNTGG 4 cut(s) 6487, 7295, 9874, 10763
XhoI CTCGAG 3 cut(s) 1078, 10936, 11601
XmnI GAANNNNTTC 5 cut(s) 678, 945, 3201, 7274, 9033
ZrmI AGTACT 7 cut(s) 62, 249, 1249, 4622, 5159, 10343, 11384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.