Rmu_sc0002741.1_g000019

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002741.1
OG0021658
Physical Location & Seq
Reverse (-)
77009 .. 78307
1299 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 411 bp
atgagatcgggatcgaccgatccggatcgcgggtcgcaggggagaggagcgatcccggtaactcctcgattgtttgcgctggccctaagggaaggattgtcctgggccatcgacaacgggtggagcaacattgatattgtatgtgataatgcttctttggtggctgctgcccatagaaatggtgttgaaacctcacttattggacaaatagtggaggaaatcaaagcttatgcaaacttacttgttgacacacagtacgcggaggtttctacgaaaatggctaatcacgtcccagaaacttcattttcgacaaggcaaagaaccggagataccagaggcacatccacatgcatacaagctagaacgggcccggtcttgtctttcgcgatgtttcgtgatgtgccaggataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

136

Amino Acids

14.61

Weight (kDa)

6.07

Isoelectric Point (pI)

23.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021658)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002741.1_g000019
rosa_roxburghii Rroxscaffold_1G00049750
rosa_samantha Rh2BG190700 Rh5BG453500 Rh5CG475800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 30, 260, 386
AccIII TCCGGA 1 cut(s) 22
AciI CCGC 2 cut(s) 30, 260
AclWI GGATC 4 cut(s) 14, 19, 33, 46
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 1 cut(s) 29
AgsI TTSAA 1 cut(s) 188
AjiI CACGTC 1 cut(s) 289
AjnI CCWGG 2 cut(s) 101, 403
AluBI AGCT 2 cut(s) 227, 359
AluI AGCT 2 cut(s) 227, 359
AlwI GGATC 4 cut(s) 14, 19, 33, 46
Aor13HI TCCGGA 1 cut(s) 22
AoxI GGCC 3 cut(s) 81, 105, 367
ApaI GGGCCC 1 cut(s) 371
ApeKI GCWGC 2 cut(s) 164, 167
AspLEI GCGC 1 cut(s) 79
AspS9I GGNCC 4 cut(s) 82, 105, 367, 368
AsuC2I CCSGG 2 cut(s) 56, 371
AxyI CCTNAGG 1 cut(s) 86
BaeGI GKGCMC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 371
BbvI GCAGC 2 cut(s) 151, 154
BccI CCATC 1 cut(s) 116
BciT130I CCWGG 2 cut(s) 103, 405
BcnI CCSGG 2 cut(s) 56, 371
BfaI CTAG 1 cut(s) 360
BisI GCNGC 2 cut(s) 165, 168
BlsI GCNGC 2 cut(s) 166, 169
Bme1390I CCNGG 4 cut(s) 56, 103, 371, 405
BmgBI CACGTC 1 cut(s) 289
BmgT120I GGNCC 4 cut(s) 82, 105, 367, 368
BmiI GGNNCC 1 cut(s) 369
BmrFI CCNGG 4 cut(s) 56, 103, 371, 405
BpuMI CCSGG 2 cut(s) 56, 371
BsaBI GATNNNNATC 2 cut(s) 10, 24
BsaJI CCNNGG 1 cut(s) 102
BsaWI WCCGGW 2 cut(s) 22, 323
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse21I CCTNAGG 1 cut(s) 86
Bse8I GATNNNNATC 2 cut(s) 10, 24
BseAI TCCGGA 1 cut(s) 22
BseBI CCWGG 2 cut(s) 103, 405
BseDI CCNNGG 1 cut(s) 102
BseGI GGATG 1 cut(s) 341
BseJI GATNNNNATC 2 cut(s) 10, 24
BseLI CCNNNNNNNGG 1 cut(s) 29
BseRI GAGGAG 2 cut(s) 54, 60
BseSI GKGCMC 1 cut(s) 371
BseXI GCAGC 2 cut(s) 151, 154
Bsh1236I CGCG 3 cut(s) 30, 260, 386
Bsh1285I CGRYCG 1 cut(s) 18
BshFI GGCC 3 cut(s) 83, 107, 369
BsiEI CGRYCG 1 cut(s) 18
BsiSI CCGG 4 cut(s) 23, 56, 324, 371
BslFI GGGAC 1 cut(s) 275
BslI CCNNNNNNNGG 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 275
BsnI GGCC 3 cut(s) 83, 107, 369
Bsp120I GGGCCC 1 cut(s) 367
Bsp1286I GDGCHC 1 cut(s) 371
Bsp13I TCCGGA 1 cut(s) 22
Bsp143I GATC 5 cut(s) 5, 11, 19, 25, 51
Bsp68I TCGCGA 1 cut(s) 386
BspACI CCGC 2 cut(s) 30, 260
BspANI GGCC 3 cut(s) 83, 107, 369
BspEI TCCGGA 1 cut(s) 22
BspFNI CGCG 3 cut(s) 30, 260, 386
BspLI GGNNCC 1 cut(s) 369
BspPI GGATC 4 cut(s) 14, 19, 33, 46
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 5 cut(s) 5, 11, 19, 25, 51
Bst2UI CCWGG 2 cut(s) 103, 405
Bst4CI ACNGT 1 cut(s) 255
BstC8I GCNNGC 1 cut(s) 81
BstDEI CTNAG 1 cut(s) 86
BstF5I GGATG 1 cut(s) 341
BstFNI CGCG 3 cut(s) 30, 260, 386
BstHHI GCGC 1 cut(s) 79
BstKTI GATC 5 cut(s) 8, 14, 22, 28, 54
BstMBI GATC 5 cut(s) 5, 11, 19, 25, 51
BstMCI CGRYCG 1 cut(s) 18
BstNI CCWGG 2 cut(s) 103, 405
BstNSI RCATGY 1 cut(s) 351
BstSCI CCNGG 4 cut(s) 54, 101, 369, 403
BstSLI GKGCMC 1 cut(s) 371
BstUI CGCG 3 cut(s) 30, 260, 386
BstV1I GCAGC 2 cut(s) 151, 154
BstXI CCANNNNNNTGG 1 cut(s) 179
Bsu36I CCTNAGG 1 cut(s) 86
BsuRI GGCC 3 cut(s) 83, 107, 369
BtgZI GCGATG 1 cut(s) 401
BtrI CACGTC 1 cut(s) 289
BtsCI GGATG 1 cut(s) 341
BtuMI TCGCGA 1 cut(s) 386
Cac8I GCNNGC 1 cut(s) 81
CfoI GCGC 1 cut(s) 79
Cfr13I GGNCC 4 cut(s) 82, 105, 367, 368
Csp6I GTAC 1 cut(s) 256
CviAII CATG 1 cut(s) 348
CviJI RGCY 7 cut(s) 83, 107, 164, 227, 281, 359, 369
CviKI_1 RGCY 7 cut(s) 83, 107, 164, 227, 281, 359, 369
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 1 cut(s) 86
DpnI GATC 5 cut(s) 7, 13, 21, 27, 53
DpnII GATC 5 cut(s) 5, 11, 19, 25, 51
Eco24I GRGCYC 1 cut(s) 371
Eco81I CCTNAGG 1 cut(s) 86
EcoRII CCWGG 2 cut(s) 101, 403
EcoT22I ATGCAT 1 cut(s) 353
EcoT38I GRGCYC 1 cut(s) 371
FaeI CATG 1 cut(s) 351
FaiI YATR 5 cut(s) 142, 174, 231, 349, 353
FaqI GGGAC 1 cut(s) 275
FatI CATG 1 cut(s) 347
FauI CCCGC 1 cut(s) 23
Fnu4HI GCNGC 2 cut(s) 165, 168
FokI GGATG 1 cut(s) 328
FriOI GRGCYC 1 cut(s) 371
Fsp4HI GCNGC 2 cut(s) 165, 168
FspBI CTAG 1 cut(s) 360
GlaI GCGC 1 cut(s) 78
GluI GCNGC 2 cut(s) 165, 168
HaeIII GGCC 3 cut(s) 83, 107, 369
HapII CCGG 4 cut(s) 23, 56, 324, 371
HhaI GCGC 1 cut(s) 79
Hin1II CATG 1 cut(s) 351
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HindIII AAGCTT 1 cut(s) 225
HpaII CCGG 4 cut(s) 23, 56, 324, 371
Hpy166II GTNNAC 1 cut(s) 247
Hpy188III TCNNGA 4 cut(s) 9, 23, 385, 395
Hpy8I GTNNAC 1 cut(s) 247
HpyAV CCTTC 1 cut(s) 86
HpyCH4III ACNGT 1 cut(s) 255
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 2 cut(s) 233, 351
HpyF3I CTNAG 1 cut(s) 86
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 1 cut(s) 351
HspAI GCGC 1 cut(s) 77
Kpn2I TCCGGA 1 cut(s) 22
Kzo9I GATC 5 cut(s) 5, 11, 19, 25, 51
LmnI GCTCC 2 cut(s) 47, 123
Lsp1109I GCAGC 2 cut(s) 151, 154
MaeI CTAG 1 cut(s) 360
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 1 cut(s) 58
MalI GATC 5 cut(s) 7, 13, 21, 27, 53
MboI GATC 5 cut(s) 5, 11, 19, 25, 51
MhlI GDGCHC 1 cut(s) 371
MnlI CCTC 6 cut(s) 38, 75, 202, 208, 256, 329
Mph1103I ATGCAT 1 cut(s) 353
MroI TCCGGA 1 cut(s) 22
MslI CAYNNNNRTG 2 cut(s) 177, 346
MspI CCGG 4 cut(s) 23, 56, 324, 371
MspR9I CCNGG 4 cut(s) 56, 103, 371, 405
MvaI CCWGG 2 cut(s) 103, 405
MvnI CGCG 3 cut(s) 30, 260, 386
NciI CCSGG 2 cut(s) 56, 371
NdeII GATC 5 cut(s) 5, 11, 19, 25, 51
NlaIII CATG 1 cut(s) 351
NlaIV GGNNCC 1 cut(s) 369
NruI TCGCGA 1 cut(s) 386
NsiI ATGCAT 1 cut(s) 353
NspI RCATGY 1 cut(s) 351
PkrI GCNGC 2 cut(s) 166, 169
Psp6I CCWGG 2 cut(s) 101, 403
PspGI CCWGG 2 cut(s) 101, 403
PspN4I GGNNCC 1 cut(s) 369
PspOMI GGGCCC 1 cut(s) 367
PspPI GGNCC 4 cut(s) 82, 105, 367, 368
PsrI GAACNNNNNNTAC 2 cut(s) 313, 345
RruI TCGCGA 1 cut(s) 386
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
RseI CAYNNNNRTG 2 cut(s) 177, 346
SatI GCNGC 2 cut(s) 165, 168
Sau3AI GATC 5 cut(s) 5, 11, 19, 25, 51
Sau96I GGNCC 4 cut(s) 82, 105, 367, 368
ScrFI CCNGG 4 cut(s) 56, 103, 371, 405
SduI GDGCHC 1 cut(s) 371
SetI ASST 5 cut(s) 194, 229, 267, 291, 361
SmiMI CAYNNNNRTG 2 cut(s) 177, 346
SsiI CCGC 2 cut(s) 30, 260
SspMI CTAG 1 cut(s) 360
StyD4I CCNGG 4 cut(s) 54, 101, 369, 403
TaaI ACNGT 1 cut(s) 255
TaiI ACGT 1 cut(s) 291
TaqI TCGA 4 cut(s) 14, 67, 111, 308
TaqII GACCGA 1 cut(s) 32
TseI GCWGC 2 cut(s) 164, 167
TspDTI ATGAA 1 cut(s) 291
XceI RCATGY 1 cut(s) 351
XspI CTAG 1 cut(s) 360
Zsp2I ATGCAT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.