Rh2BG190700

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0021658
Physical Location & Seq
Forward (+)
17135040 .. 17143919
8880 bp
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UTR
Exon/CDS
Intron
Rh2BG190700.1

Sequence Viewer

Length: 402 bp
ATGGTGGCTATACCATATCAAATATTCTCATTTAAACATTTATATGTAACAAATAACATACTAATCGATGTGGAAGCGCTGGCGCTAAGGGAAGGATTGTCCTGGGCCATCGACAACGGGTGGAGCAACATTGATATTGTATGTGATAATGCTTCTTTGGTGGCTGCTGCCCATAGAAATGGTGTTGAAACCTCACTTATTGGACAAATAGTGGAGGAAATCAAAGCTTATGCAAACTTACTTGTTGACACACAGTACGCGGAGGTTTCTACGAAAATGGCTAATGGCGTCCCAGAAACTTCATTTTCGACAAGGCAAAGAACCGGAGATACCAGAGGCACATCCACATGCATACAAGCTAGAACGGGCCCGATCTTTACTTCCATGGATGATGAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

133

Amino Acids

14.57

Weight (kDa)

4.61

Isoelectric Point (pI)

20.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 24 - 78 2.1e-06 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021658)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002741.1_g000019
rosa_roxburghii Rroxscaffold_1G00049750
rosa_samantha Rh2BG190700 Rh5BG453500 Rh5CG475800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 260
AciI CCGC 1 cut(s) 260
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 1 cut(s) 257
AfeI AGCGCT 1 cut(s) 78
AgsI TTSAA 1 cut(s) 188
AjnI CCWGG 1 cut(s) 101
AluBI AGCT 2 cut(s) 227, 359
AluI AGCT 2 cut(s) 227, 359
Aor51HI AGCGCT 1 cut(s) 78
AoxI GGCC 2 cut(s) 105, 367
ApaI GGGCCC 1 cut(s) 371
ApeKI GCWGC 2 cut(s) 164, 167
AspLEI GCGC 2 cut(s) 79, 85
AspS9I GGNCC 3 cut(s) 105, 367, 368
BaeGI GKGCMC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 371
BbvI GCAGC 2 cut(s) 151, 154
BccI CCATC 1 cut(s) 116
BciT130I CCWGG 1 cut(s) 103
BfaI CTAG 1 cut(s) 360
BfoI RGCGCY 2 cut(s) 80, 86
BisI GCNGC 2 cut(s) 165, 168
BlsI GCNGC 2 cut(s) 166, 169
Bme1390I CCNGG 1 cut(s) 103
BmgT120I GGNCC 3 cut(s) 105, 367, 368
BmiI GGNNCC 1 cut(s) 369
BmrFI CCNGG 1 cut(s) 103
Bpu10I CCTNAGC 1 cut(s) 86
Bsa29I ATCGAT 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 288
BsaJI CCNNGG 2 cut(s) 102, 384
BsaWI WCCGGW 1 cut(s) 323
BseBI CCWGG 1 cut(s) 103
BseCI ATCGAT 1 cut(s) 66
BseDI CCNNGG 2 cut(s) 102, 384
BseGI GGATG 2 cut(s) 341, 394
BseSI GKGCMC 1 cut(s) 371
BseXI GCAGC 2 cut(s) 151, 154
Bsh1236I CGCG 1 cut(s) 260
BshFI GGCC 2 cut(s) 107, 369
BshVI ATCGAT 1 cut(s) 66
BsiSI CCGG 1 cut(s) 324
BslFI GGGAC 1 cut(s) 275
BsmFI GGGAC 1 cut(s) 275
BsnI GGCC 2 cut(s) 107, 369
Bsp120I GGGCCC 1 cut(s) 367
Bsp1286I GDGCHC 1 cut(s) 371
Bsp143I GATC 1 cut(s) 372
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 1 cut(s) 260
BspANI GGCC 2 cut(s) 107, 369
BspDI ATCGAT 1 cut(s) 66
BspFNI CGCG 1 cut(s) 260
BspLI GGNNCC 1 cut(s) 369
BssECI CCNNGG 2 cut(s) 102, 384
BssMI GATC 1 cut(s) 372
BssNI GRCGYC 1 cut(s) 288
BssT1I CCWWGG 1 cut(s) 384
Bst2UI CCWGG 1 cut(s) 103
Bst4CI ACNGT 1 cut(s) 255
BstACI GRCGYC 1 cut(s) 288
BstC8I GCNNGC 1 cut(s) 81
BstDEI CTNAG 1 cut(s) 86
BstDSI CCRYGG 1 cut(s) 384
BstF5I GGATG 2 cut(s) 341, 394
BstFNI CGCG 1 cut(s) 260
BstH2I RGCGCY 2 cut(s) 80, 86
BstHHI GCGC 2 cut(s) 79, 85
BstKTI GATC 1 cut(s) 375
BstMBI GATC 1 cut(s) 372
BstNI CCWGG 1 cut(s) 103
BstNSI RCATGY 1 cut(s) 351
BstSCI CCNGG 1 cut(s) 101
BstSLI GKGCMC 1 cut(s) 371
BstUI CGCG 1 cut(s) 260
BstV1I GCAGC 2 cut(s) 151, 154
BstXI CCANNNNNNTGG 1 cut(s) 179
Bsu15I ATCGAT 1 cut(s) 66
BsuRI GGCC 2 cut(s) 107, 369
BsuTUI ATCGAT 1 cut(s) 66
BtgI CCRYGG 1 cut(s) 384
BtsCI GGATG 2 cut(s) 341, 394
Cac8I GCNNGC 1 cut(s) 81
CfoI GCGC 2 cut(s) 79, 85
Cfr13I GGNCC 3 cut(s) 105, 367, 368
ClaI ATCGAT 1 cut(s) 66
CseI GACGC 1 cut(s) 277
Csp6I GTAC 1 cut(s) 256
CviAII CATG 2 cut(s) 348, 385
CviJI RGCY 7 cut(s) 8, 107, 164, 227, 281, 359, 369
CviKI_1 RGCY 7 cut(s) 8, 107, 164, 227, 281, 359, 369
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 1 cut(s) 86
DpnI GATC 1 cut(s) 374
DpnII GATC 1 cut(s) 372
DraI TTTAAA 1 cut(s) 34
Eco130I CCWWGG 1 cut(s) 384
Eco24I GRGCYC 1 cut(s) 371
Eco47III AGCGCT 1 cut(s) 78
EcoRII CCWGG 1 cut(s) 101
EcoT14I CCWWGG 1 cut(s) 384
EcoT22I ATGCAT 1 cut(s) 353
EcoT38I GRGCYC 1 cut(s) 371
ErhI CCWWGG 1 cut(s) 384
FaeI CATG 2 cut(s) 351, 388
FaqI GGGAC 1 cut(s) 275
FatI CATG 2 cut(s) 347, 384
Fnu4HI GCNGC 2 cut(s) 165, 168
FokI GGATG 1 cut(s) 328
FriOI GRGCYC 1 cut(s) 371
Fsp4HI GCNGC 2 cut(s) 165, 168
FspBI CTAG 1 cut(s) 360
GlaI GCGC 2 cut(s) 78, 84
GluI GCNGC 2 cut(s) 165, 168
HaeII RGCGCY 2 cut(s) 80, 86
HaeIII GGCC 2 cut(s) 107, 369
HapII CCGG 1 cut(s) 324
HgaI GACGC 1 cut(s) 277
HhaI GCGC 2 cut(s) 79, 85
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 2 cut(s) 351, 388
Hin6I GCGC 2 cut(s) 77, 83
HinP1I GCGC 2 cut(s) 77, 83
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HindIII AAGCTT 1 cut(s) 225
HpaII CCGG 1 cut(s) 324
Hpy166II GTNNAC 1 cut(s) 247
Hpy8I GTNNAC 1 cut(s) 247
HpyAV CCTTC 1 cut(s) 86
HpyCH4III ACNGT 1 cut(s) 255
HpyCH4V TGCA 2 cut(s) 233, 351
HpyF3I CTNAG 1 cut(s) 86
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 2 cut(s) 351, 388
HspAI GCGC 2 cut(s) 77, 83
Kzo9I GATC 1 cut(s) 372
LmnI GCTCC 1 cut(s) 123
LpnPI CCDG 6 cut(s) 65, 88, 115, 306, 337, 346
Lsp1109I GCAGC 2 cut(s) 151, 154
MaeI CTAG 1 cut(s) 360
MaeIII GTNAC 1 cut(s) 46
MalI GATC 1 cut(s) 374
MboI GATC 1 cut(s) 372
MhlI GDGCHC 1 cut(s) 371
MluCI AATT 1 cut(s) 397
MnlI CCTC 4 cut(s) 202, 208, 256, 329
Mph1103I ATGCAT 1 cut(s) 353
MseI TTAA 2 cut(s) 33, 400
MslI CAYNNNNRTG 3 cut(s) 42, 177, 346
MspI CCGG 1 cut(s) 324
MspR9I CCNGG 1 cut(s) 103
MvaI CCWGG 1 cut(s) 103
MvnI CGCG 1 cut(s) 260
NcoI CCATGG 1 cut(s) 384
NdeII GATC 1 cut(s) 372
NlaIII CATG 2 cut(s) 351, 388
NlaIV GGNNCC 1 cut(s) 369
NsiI ATGCAT 1 cut(s) 353
NspI RCATGY 1 cut(s) 351
PkrI GCNGC 2 cut(s) 166, 169
Psp6I CCWGG 1 cut(s) 101
PspGI CCWGG 1 cut(s) 101
PspN4I GGNNCC 1 cut(s) 369
PspOMI GGGCCC 1 cut(s) 367
PspPI GGNCC 3 cut(s) 105, 367, 368
PsrI GAACNNNNNNTAC 2 cut(s) 313, 345
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
RseI CAYNNNNRTG 3 cut(s) 42, 177, 346
SaqAI TTAA 2 cut(s) 33, 400
SatI GCNGC 2 cut(s) 165, 168
Sau3AI GATC 1 cut(s) 372
Sau96I GGNCC 3 cut(s) 105, 367, 368
ScrFI CCNGG 1 cut(s) 103
SduI GDGCHC 1 cut(s) 371
SetI ASST 4 cut(s) 194, 229, 267, 361
SmiMI CAYNNNNRTG 3 cut(s) 42, 177, 346
Sse9I AATT 1 cut(s) 397
SsiI CCGC 1 cut(s) 260
SspI AATATT 1 cut(s) 24
SspMI CTAG 1 cut(s) 360
StyD4I CCNGG 1 cut(s) 101
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 1 cut(s) 255
TaqI TCGA 3 cut(s) 66, 111, 308
TasI AATT 1 cut(s) 397
Tru1I TTAA 2 cut(s) 33, 400
Tru9I TTAA 2 cut(s) 33, 400
TseI GCWGC 2 cut(s) 164, 167
TspDTI ATGAA 1 cut(s) 291
XceI RCATGY 1 cut(s) 351
XspI CTAG 1 cut(s) 360
Zsp2I ATGCAT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.