Rmu_sc0002908.1_g000026

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002908.1
OG0025547
Physical Location & Seq
Forward (+)
117210 .. 118354
1145 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 513 bp
atgtctgactctttgtgtcagcgatcttggggagacatcattgctgcagctgttctttctggaaaccgaaactttgaaggtcgtattcactcgttaataagagctaactatcttgcttcgcctccattggttgttgcctacgcacatgctggcacggttgacattaatttcgatcaagagccaattggaaaagggaaggataggaagagtgtgtacttgaaggatatctggcctagtaatgaagaaattgcacaggtatttcaatcttttgtcttacgtgatatgtttaagaacacatatgaggccatggttagtttgtggaatgagctctctgtcccttcttcgactctttattcttgggatgcaaactcgacctacattcacaagccctcatatttaaagaacatgagcatggaactaccaggtcctcgtggcgtaaaggatgcatatatattgtttgctgtatttaggcgacagtataaccacaatcatattgttaggaatttgacctaa

Protein Analysis

170

Amino Acids

19.4

Weight (kDa)

8.65

Isoelectric Point (pI)

43.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aconitase PF00330 16 - 51 3.4e-10 Aconitase family (aconitate hydratase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0025547)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8003062.1_g000001 Rmu_sc0002908.1_g000026 Rmu_sc0023758.1_g000002

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 502
AfaI GTAC 1 cut(s) 215
AgsI TTSAA 3 cut(s) 77, 220, 263
AjnI CCWGG 1 cut(s) 421
AluBI AGCT 3 cut(s) 50, 104, 328
AluI AGCT 3 cut(s) 50, 104, 328
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 1 cut(s) 27
AoxI GGCC 2 cut(s) 230, 303
ApeKI GCWGC 2 cut(s) 44, 47
ApoI RAATTY 1 cut(s) 502
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
AseI ATTAAT 1 cut(s) 165
AspS9I GGNCC 1 cut(s) 425
AvaII GGWCC 1 cut(s) 425
BanII GRGCYC 1 cut(s) 330
BarI GAAGNNNNNNTAC 2 cut(s) 197, 229
BauI CACGAG 1 cut(s) 429
Bbv12I GWGCWC 1 cut(s) 330
BbvI GCAGC 2 cut(s) 31, 59
BciT130I CCWGG 1 cut(s) 423
BcoDI GTCTC 1 cut(s) 27
BfaI CTAG 1 cut(s) 234
BfmI CTRYAG 1 cut(s) 45
BisI GCNGC 2 cut(s) 45, 48
BlsI GCNGC 2 cut(s) 46, 49
Bme1390I CCNGG 1 cut(s) 423
Bme18I GGWCC 1 cut(s) 425
BmgT120I GGNCC 1 cut(s) 425
BmrFI CCNGG 1 cut(s) 423
BmsI GCATC 2 cut(s) 352, 433
BsaAI YACGTR 1 cut(s) 278
BsaJI CCNNGG 1 cut(s) 306
Bse3DI GCAATG 1 cut(s) 39
BseBI CCWGG 1 cut(s) 423
BseDI CCNNGG 1 cut(s) 306
BseGI GGATG 2 cut(s) 367, 448
BseMI GCAATG 1 cut(s) 39
BseXI GCAGC 2 cut(s) 31, 59
BshFI GGCC 2 cut(s) 232, 305
BsiHKAI GWGCWC 1 cut(s) 330
BslFI GGGAC 1 cut(s) 320
BsmAI GTCTC 1 cut(s) 27
BsmFI GGGAC 1 cut(s) 320
BsnI GGCC 2 cut(s) 232, 305
Bsp1286I GDGCHC 1 cut(s) 330
Bsp143I GATC 2 cut(s) 23, 172
Bsp19I CCATGG 1 cut(s) 306
BspANI GGCC 2 cut(s) 232, 305
BspMAI CTGCAG 1 cut(s) 49
BsrDI GCAATG 1 cut(s) 39
BssECI CCNNGG 1 cut(s) 306
BssMI GATC 2 cut(s) 23, 172
BssSI CACGAG 1 cut(s) 429
BssT1I CCWWGG 1 cut(s) 306
Bst2BI CACGAG 1 cut(s) 429
Bst2UI CCWGG 1 cut(s) 423
Bst4CI ACNGT 2 cut(s) 157, 477
Bst6I CTCTTC 1 cut(s) 200
BstBAI YACGTR 1 cut(s) 278
BstC8I GCNNGC 1 cut(s) 151
BstDSI CCRYGG 1 cut(s) 306
BstF5I GGATG 2 cut(s) 367, 448
BstKTI GATC 2 cut(s) 26, 175
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 2 cut(s) 23, 172
BstNI CCWGG 1 cut(s) 423
BstNSI RCATGY 1 cut(s) 149
BstSCI CCNGG 1 cut(s) 421
BstSFI CTRYAG 1 cut(s) 45
BstV1I GCAGC 2 cut(s) 31, 59
BsuRI GGCC 2 cut(s) 232, 305
BtgI CCRYGG 1 cut(s) 306
BtsCI GGATG 2 cut(s) 367, 448
Cac8I GCNNGC 1 cut(s) 151
Cfr13I GGNCC 1 cut(s) 425
CsiI ACCWGGT 1 cut(s) 421
Csp6I GTAC 1 cut(s) 214
CviAII CATG 4 cut(s) 146, 307, 406, 412
CviJI RGCY 7 cut(s) 50, 104, 181, 232, 305, 328, 388
CviKI_1 RGCY 7 cut(s) 50, 104, 181, 232, 305, 328, 388
CviQI GTAC 1 cut(s) 214
DpnI GATC 2 cut(s) 25, 174
DpnII GATC 2 cut(s) 23, 172
DraI TTTAAA 1 cut(s) 399
Eam1104I CTCTTC 1 cut(s) 200
EarI CTCTTC 1 cut(s) 200
Ecl136II GAGCTC 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 330
Eco32I GATATC 1 cut(s) 226
Eco47I GGWCC 1 cut(s) 425
Eco53kI GAGCTC 1 cut(s) 328
EcoICRI GAGCTC 1 cut(s) 328
EcoO109I RGGNCCY 1 cut(s) 425
EcoRII CCWGG 1 cut(s) 421
EcoRV GATATC 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 306
EcoT22I ATGCAT 1 cut(s) 448
EcoT38I GRGCYC 1 cut(s) 330
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 4 cut(s) 149, 310, 409, 415
FaqI GGGAC 1 cut(s) 320
FatI CATG 4 cut(s) 145, 306, 405, 411
FauNDI CATATG 1 cut(s) 298
Fnu4HI GCNGC 2 cut(s) 45, 48
FokI GGATG 2 cut(s) 374, 455
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 2 cut(s) 45, 48
FspBI CTAG 1 cut(s) 234
GluI GCNGC 2 cut(s) 45, 48
HaeIII GGCC 2 cut(s) 232, 305
Hin1II CATG 4 cut(s) 149, 310, 409, 415
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 2 cut(s) 8, 346
Hpy166II GTNNAC 2 cut(s) 160, 214
Hpy188I TCNGA 1 cut(s) 7
Hpy188III TCNNGA 2 cut(s) 60, 176
Hpy8I GTNNAC 2 cut(s) 160, 214
HpyAV CCTTC 4 cut(s) 71, 190, 214, 348
HpyCH4III ACNGT 2 cut(s) 157, 477
HpyCH4IV ACGT 1 cut(s) 277
HpyCH4V TGCA 4 cut(s) 47, 251, 365, 446
HpySE526I ACGT 1 cut(s) 277
Hsp92II CATG 4 cut(s) 149, 310, 409, 415
Kzo9I GATC 2 cut(s) 23, 172
LpnPI CCDG 6 cut(s) 45, 135, 214, 239, 408, 435
Lsp1109I GCAGC 2 cut(s) 31, 59
LweI GCATC 2 cut(s) 352, 433
MabI ACCWGGT 1 cut(s) 421
MaeI CTAG 1 cut(s) 234
MaeII ACGT 1 cut(s) 277
MalI GATC 2 cut(s) 25, 174
MboI GATC 2 cut(s) 23, 172
MboII GAAGA 3 cut(s) 217, 254, 333
MfeI CAATTG 1 cut(s) 183
MhlI GDGCHC 1 cut(s) 330
MluCI AATT 4 cut(s) 166, 183, 246, 502
MlyI GAGTC 2 cut(s) 2, 340
MnlI CCTC 4 cut(s) 132, 295, 400, 438
Mph1103I ATGCAT 1 cut(s) 448
MseI TTAA 4 cut(s) 95, 165, 288, 398
MslI CAYNNNNRTG 1 cut(s) 410
MspA1I CMGCKG 1 cut(s) 50
MspR9I CCNGG 1 cut(s) 423
MunI CAATTG 1 cut(s) 183
MvaI CCWGG 1 cut(s) 423
NcoI CCATGG 1 cut(s) 306
NdeI CATATG 1 cut(s) 298
NdeII GATC 2 cut(s) 23, 172
NlaIII CATG 4 cut(s) 149, 310, 409, 415
NsiI ATGCAT 1 cut(s) 448
NspI RCATGY 1 cut(s) 149
PkrI GCNGC 2 cut(s) 46, 49
PleI GAGTC 2 cut(s) 2, 340
PpsI GAGTC 2 cut(s) 2, 340
Ppu21I YACGTR 1 cut(s) 278
PpuMI RGGWCCY 1 cut(s) 425
PshBI ATTAAT 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 330
Psp5II RGGWCCY 1 cut(s) 425
Psp6I CCWGG 1 cut(s) 421
PspGI CCWGG 1 cut(s) 421
PspPI GGNCC 1 cut(s) 425
PspPPI RGGWCCY 1 cut(s) 425
PstI CTGCAG 1 cut(s) 49
PvuII CAGCTG 1 cut(s) 50
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
RseI CAYNNNNRTG 1 cut(s) 410
SacI GAGCTC 1 cut(s) 330
SaqAI TTAA 4 cut(s) 95, 165, 288, 398
SatI GCNGC 2 cut(s) 45, 48
Sau3AI GATC 2 cut(s) 23, 172
Sau96I GGNCC 1 cut(s) 425
SchI GAGTC 2 cut(s) 2, 340
ScrFI CCNGG 1 cut(s) 423
SduI GDGCHC 1 cut(s) 330
SetI ASST 9 cut(s) 52, 82, 106, 258, 280, 330, 377, 427, 512
SexAI ACCWGGT 1 cut(s) 421
SfaNI GCATC 2 cut(s) 352, 433
SfcI CTRYAG 1 cut(s) 45
SinI GGWCC 1 cut(s) 425
SmiMI CAYNNNNRTG 1 cut(s) 410
Sse9I AATT 4 cut(s) 166, 183, 246, 502
SspMI CTAG 1 cut(s) 234
SstI GAGCTC 1 cut(s) 330
StyD4I CCNGG 1 cut(s) 421
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 2 cut(s) 157, 477
TaiI ACGT 1 cut(s) 280
TaqI TCGA 3 cut(s) 171, 344, 371
TasI AATT 4 cut(s) 166, 183, 246, 502
TatI WGTACW 1 cut(s) 213
Tru1I TTAA 4 cut(s) 95, 165, 288, 398
Tru9I TTAA 4 cut(s) 95, 165, 288, 398
TseI GCWGC 2 cut(s) 44, 47
TspDTI ATGAA 1 cut(s) 255
VpaK11BI GGWCC 1 cut(s) 425
VspI ATTAAT 1 cut(s) 165
XapI RAATTY 1 cut(s) 502
XceI RCATGY 1 cut(s) 149
XspI CTAG 1 cut(s) 234
Zsp2I ATGCAT 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.