Rmu_sc0003131.1_g000038

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003131.1
OG0022229
Physical Location & Seq
Forward (+)
186336 .. 186596
261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003131.1_g000038.1.cds

Sequence Viewer

Length: 261 bp
atggtgtcttacgagtacgttctgggtggagtcttggcttctctgctcggctctgttttcctcatgatcatctgcagcacttttagcgccaagaaaaaggtggaggatttgaaaactaataatgggtttgtaaggatgccgcagaacggtgcttttcggccggagatggaggaggaggagaaaagtaccgacgtcgtcattgtcggcgccggagttgccggtgcagctcttgcttacactcttgccaaggtcagattgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

86

Amino Acids

9.23

Weight (kDa)

6.2

Isoelectric Point (pI)

22.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022229)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g03871
rosa_multiflora Rmu_sc0001293.1_g000028 Rmu_sc0003131.1_g000038
rosa_samantha Rh6BG031200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 195
AccB1I GGYRCC 1 cut(s) 206
AciI CCGC 1 cut(s) 140
AcoI YGGCCR 1 cut(s) 158
AcyI GRCGYC 2 cut(s) 192, 207
AfaI GTAC 2 cut(s) 17, 187
AfiI CCNNNNNNNGG 1 cut(s) 146
AgsI TTSAA 1 cut(s) 112
AluBI AGCT 1 cut(s) 227
AluI AGCT 1 cut(s) 227
AoxI GGCC 1 cut(s) 158
ApeKI GCWGC 2 cut(s) 75, 224
AspLEI GCGC 2 cut(s) 89, 209
BanI GGYRCC 1 cut(s) 206
BbvI GCAGC 2 cut(s) 87, 236
BccI CCATC 1 cut(s) 160
BclI TGATCA 1 cut(s) 66
BfmI CTRYAG 1 cut(s) 73
BfoI RGCGCY 2 cut(s) 90, 210
BisI GCNGC 3 cut(s) 76, 140, 225
BlsI GCNGC 3 cut(s) 77, 141, 226
BmiI GGNNCC 1 cut(s) 208
BmsI GCATC 1 cut(s) 126
BsaHI GRCGYC 2 cut(s) 192, 207
BsaJI CCNNGG 1 cut(s) 246
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse118I RCCGGY 1 cut(s) 218
BseDI CCNNGG 1 cut(s) 246
BseGI GGATG 1 cut(s) 141
BseLI CCNNNNNNNGG 1 cut(s) 146
BseRI GAGGAG 3 cut(s) 185, 188, 191
BseX3I CGGCCG 1 cut(s) 158
BseXI GCAGC 2 cut(s) 87, 236
BsgI GTGCAG 1 cut(s) 243
Bsh1285I CGRYCG 1 cut(s) 161
BshFI GGCC 1 cut(s) 160
BshNI GGYRCC 1 cut(s) 206
BsiEI CGRYCG 1 cut(s) 161
BsiSI CCGG 3 cut(s) 161, 210, 219
BslI CCNNNNNNNGG 1 cut(s) 146
BsnI GGCC 1 cut(s) 160
Bsp143I GATC 1 cut(s) 66
BspACI CCGC 1 cut(s) 140
BspANI GGCC 1 cut(s) 160
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 1 cut(s) 208
BspMAI CTGCAG 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 206
BsrFI RCCGGY 1 cut(s) 218
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 1 cut(s) 246
BssMI GATC 1 cut(s) 66
BssNI GRCGYC 2 cut(s) 192, 207
BssT1I CCWWGG 1 cut(s) 246
Bst4CI ACNGT 1 cut(s) 149
BstACI GRCGYC 2 cut(s) 192, 207
BstAPI GCANNNNNTGC 1 cut(s) 230
BstF5I GGATG 1 cut(s) 141
BstH2I RGCGCY 2 cut(s) 90, 210
BstHHI GCGC 2 cut(s) 89, 209
BstKTI GATC 1 cut(s) 69
BstMBI GATC 1 cut(s) 66
BstMCI CGRYCG 1 cut(s) 161
BstMWI GCNNNNNNNGC 4 cut(s) 84, 215, 224, 230
BstSFI CTRYAG 1 cut(s) 73
BstV1I GCAGC 2 cut(s) 87, 236
BstZI CGGCCG 1 cut(s) 158
BsuRI GGCC 1 cut(s) 160
BtsCI GGATG 1 cut(s) 141
CciI TCATGA 1 cut(s) 63
CfoI GCGC 2 cut(s) 89, 209
Cfr10I RCCGGY 1 cut(s) 218
Csp6I GTAC 2 cut(s) 16, 186
CviAII CATG 1 cut(s) 64
CviJI RGCY 4 cut(s) 38, 51, 160, 227
CviKI_1 RGCY 4 cut(s) 38, 51, 160, 227
CviQI GTAC 2 cut(s) 16, 186
DinI GGCGCC 1 cut(s) 208
DpnI GATC 1 cut(s) 68
DpnII GATC 1 cut(s) 66
EaeI YGGCCR 1 cut(s) 158
EagI CGGCCG 1 cut(s) 158
EclXI CGGCCG 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 246
Eco52I CGGCCG 1 cut(s) 158
EcoT14I CCWWGG 1 cut(s) 246
EgeI GGCGCC 1 cut(s) 208
EheI GGCGCC 1 cut(s) 208
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 1 cut(s) 67
FaiI YATR 1 cut(s) 65
FatI CATG 1 cut(s) 63
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 3 cut(s) 76, 140, 225
FokI GGATG 1 cut(s) 148
Fsp4HI GCNGC 3 cut(s) 76, 140, 225
GlaI GCGC 2 cut(s) 88, 208
GluI GCNGC 3 cut(s) 76, 140, 225
HaeII RGCGCY 2 cut(s) 90, 210
HaeIII GGCC 1 cut(s) 160
HapII CCGG 3 cut(s) 161, 210, 219
HhaI GCGC 2 cut(s) 89, 209
Hin1I GRCGYC 2 cut(s) 192, 207
Hin1II CATG 1 cut(s) 67
Hin6I GCGC 2 cut(s) 87, 207
HinP1I GCGC 2 cut(s) 87, 207
HinfI GANTC 1 cut(s) 30
HpaII CCGG 3 cut(s) 161, 210, 219
Hpy188I TCNGA 1 cut(s) 254
Hpy188III TCNNGA 1 cut(s) 64
Hpy99I CGWCG 2 cut(s) 194, 197
HpyCH4III ACNGT 1 cut(s) 149
HpyCH4IV ACGT 2 cut(s) 18, 192
HpyCH4V TGCA 2 cut(s) 75, 224
HpyF10VI GCNNNNNNNGC 4 cut(s) 84, 215, 224, 230
HpySE526I ACGT 2 cut(s) 18, 192
Hsp92I GRCGYC 2 cut(s) 192, 207
Hsp92II CATG 1 cut(s) 67
HspAI GCGC 2 cut(s) 87, 207
KasI GGCGCC 1 cut(s) 206
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 1 cut(s) 66
LpnPI CCDG 4 cut(s) 8, 174, 223, 232
Lsp1109I GCAGC 2 cut(s) 87, 236
LweI GCATC 1 cut(s) 126
MaeII ACGT 2 cut(s) 18, 192
MalI GATC 1 cut(s) 68
MboI GATC 1 cut(s) 66
Mly113I GGCGCC 1 cut(s) 207
MlyI GAGTC 1 cut(s) 39
MnlI CCTC 5 cut(s) 71, 97, 163, 166, 169
MspI CCGG 3 cut(s) 161, 210, 219
MwoI GCNNNNNNNGC 4 cut(s) 84, 215, 224, 230
NarI GGCGCC 1 cut(s) 207
NdeII GATC 1 cut(s) 66
NlaIII CATG 1 cut(s) 67
NlaIV GGNNCC 1 cut(s) 208
NmeAIII GCCGAG 1 cut(s) 27
PagI TCATGA 1 cut(s) 63
PflFI GACNNNGTC 1 cut(s) 194
PkrI GCNGC 3 cut(s) 77, 141, 226
PleI GAGTC 1 cut(s) 38
PluTI GGCGCC 1 cut(s) 210
PpsI GAGTC 1 cut(s) 38
PspN4I GGNNCC 1 cut(s) 208
PstI CTGCAG 1 cut(s) 77
PsyI GACNNNGTC 1 cut(s) 194
RsaI GTAC 2 cut(s) 17, 187
RsaNI GTAC 2 cut(s) 16, 186
SatI GCNGC 3 cut(s) 76, 140, 225
Sau3AI GATC 1 cut(s) 66
SchI GAGTC 1 cut(s) 39
SetI ASST 5 cut(s) 21, 102, 195, 229, 252
SfaNI GCATC 1 cut(s) 126
SfcI CTRYAG 1 cut(s) 73
SfoI GGCGCC 1 cut(s) 208
SsiI CCGC 1 cut(s) 140
SspDI GGCGCC 1 cut(s) 206
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 1 cut(s) 149
TaiI ACGT 2 cut(s) 21, 195
TauI GCSGC 1 cut(s) 142
TseI GCWGC 2 cut(s) 75, 224
Tth111I GACNNNGTC 1 cut(s) 194
XcmI CCANNNNNNNNNTGG 1 cut(s) 97
ZraI GACGTC 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.