Rmu_sc0003244.1_g000025

expansin-A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003244.1
OG0025555
Physical Location & Seq
Reverse (-)
90687 .. 91491
805 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 612 bp
atgtctttgagagctttgtgttggtcgttccttgtcttcttttttgttgtctatgtcaattctcatgatcatcataagccaaagcctcatgatcagcataagccaaagcctcatgaccagcagaagccaaggcctgatggtcagcagaagccaaggcctgatgatcagcagaagccaacgtttgatgatcagcagaagccaaagttgcagtcaactgaatggaaacatgctcatgccacattctatgaaggaggttccggaacatttggtggagcttgtggataccatgatgtagttcaagaaggttatggcctggagacagtagcattgagcaatgcattgttcaacaagggggagatgtgtggtgcatgcttcgaaatcaaatgcgaggactcgcctcaatggtgcaagcctggccagccaagtctcattgttacagcaaccaaccagtgccctcctaattacaaccaaccgagtgataacggaggatggtgcaacgaaccccgtgaacatttcgacatagcaaggcctgtgttcgagactattgctgcgtacaaggccggcatcgttccagtctcataccgcaggtacaatatatatataccgatatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

203

Amino Acids

23.05

Weight (kDa)

6.78

Isoelectric Point (pI)

44.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 79 - 167 3.3e-08 EXPB1-like domain 1
DPBB_1 PF03330 107 - 194 1.6e-19 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025555)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003244.1_g000025
rosa_roxburghii Rroxscaffold_2G00105040
rosa_samantha Rh2BG422200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 576
AccIII TCCGGA 1 cut(s) 257
AciI CCGC 1 cut(s) 583
AclI AACGTT 1 cut(s) 179
AcoI YGGCCR 1 cut(s) 415
AfaI GTAC 2 cut(s) 554, 590
AgsI TTSAA 2 cut(s) 299, 346
AjnI CCWGG 2 cut(s) 312, 412
AluBI AGCT 2 cut(s) 14, 275
AluI AGCT 2 cut(s) 14, 275
Alw26I GTCTC 4 cut(s) 311, 431, 533, 580
Aor13HI TCCGGA 1 cut(s) 257
AoxI GGCC 6 cut(s) 131, 155, 310, 415, 527, 558
ApeKI GCWGC 1 cut(s) 548
ArsI GACNNNNNNTTYG 2 cut(s) 194, 226
AsuII TTCGAA 1 cut(s) 375
BaeGI GKGCMC 1 cut(s) 455
BalI TGGCCA 1 cut(s) 417
BbsI GAAGAC 1 cut(s) 28
BbvI GCAGC 1 cut(s) 535
BccI CCATC 2 cut(s) 131, 483
BcgI CGANNNNNNTGC 2 cut(s) 527, 561
BciT130I CCWGG 2 cut(s) 314, 414
BciVI GTATCC 1 cut(s) 275
BclI TGATCA 4 cut(s) 67, 91, 163, 187
BcoDI GTCTC 4 cut(s) 311, 431, 533, 580
BfuAI ACCTGC 1 cut(s) 576
BfuI GTATCC 1 cut(s) 275
BisI GCNGC 1 cut(s) 549
BlsI GCNGC 1 cut(s) 550
Bme1390I CCNGG 2 cut(s) 314, 414
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 2 cut(s) 314, 414
BmsI GCATC 1 cut(s) 573
BpiI GAAGAC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 335
Bpu14I TTCGAA 1 cut(s) 375
BsaJI CCNNGG 2 cut(s) 128, 152
BsaWI WCCGGW 1 cut(s) 257
Bse118I RCCGGY 1 cut(s) 560
Bse1I ACTGG 2 cut(s) 448, 572
Bse3DI GCAATG 1 cut(s) 340
BseAI TCCGGA 1 cut(s) 257
BseBI CCWGG 2 cut(s) 314, 414
BseDI CCNNGG 2 cut(s) 128, 152
BseGI GGATG 1 cut(s) 494
BseMI GCAATG 1 cut(s) 340
BseNI ACTGG 2 cut(s) 448, 572
BseSI GKGCMC 1 cut(s) 455
BseXI GCAGC 1 cut(s) 535
BshFI GGCC 6 cut(s) 133, 157, 312, 417, 529, 560
BsiSI CCGG 2 cut(s) 258, 561
BsmAI GTCTC 4 cut(s) 311, 431, 533, 580
BsnI GGCC 6 cut(s) 133, 157, 312, 417, 529, 560
Bsp119I TTCGAA 1 cut(s) 375
Bsp1286I GDGCHC 1 cut(s) 455
Bsp13I TCCGGA 1 cut(s) 257
Bsp143I GATC 4 cut(s) 67, 91, 163, 187
BspACI CCGC 1 cut(s) 583
BspANI GGCC 6 cut(s) 133, 157, 312, 417, 529, 560
BspEI TCCGGA 1 cut(s) 257
BspHI TCATGA 3 cut(s) 64, 88, 112
BspLI GGNNCC 1 cut(s) 256
BspMI ACCTGC 1 cut(s) 576
BspT104I TTCGAA 1 cut(s) 375
BsrDI GCAATG 1 cut(s) 340
BsrFI RCCGGY 1 cut(s) 560
BsrI ACTGG 2 cut(s) 448, 572
BssAI RCCGGY 1 cut(s) 560
BssECI CCNNGG 2 cut(s) 128, 152
BssMI GATC 4 cut(s) 67, 91, 163, 187
BssT1I CCWWGG 2 cut(s) 128, 152
Bst2UI CCWGG 2 cut(s) 314, 414
Bst4CI ACNGT 1 cut(s) 322
BstBI TTCGAA 1 cut(s) 375
BstC8I GCNNGC 4 cut(s) 370, 410, 419, 562
BstF5I GGATG 1 cut(s) 494
BstKTI GATC 4 cut(s) 70, 94, 166, 190
BstMAI GTCTC 4 cut(s) 311, 431, 533, 580
BstMBI GATC 4 cut(s) 67, 91, 163, 187
BstMWI GCNNNNNNNGC 4 cut(s) 205, 414, 418, 557
BstNI CCWGG 2 cut(s) 314, 414
BstNSI RCATGY 2 cut(s) 230, 372
BstSCI CCNGG 2 cut(s) 312, 412
BstSLI GKGCMC 1 cut(s) 455
BstV1I GCAGC 1 cut(s) 535
BstV2I GAAGAC 1 cut(s) 28
BsuI GTATCC 1 cut(s) 275
BsuRI GGCC 6 cut(s) 133, 157, 312, 417, 529, 560
BtsCI GGATG 1 cut(s) 494
BtsIMutI CAGTG 1 cut(s) 455
BveI ACCTGC 1 cut(s) 576
Cac8I GCNNGC 4 cut(s) 370, 410, 419, 562
CciI TCATGA 3 cut(s) 64, 88, 112
Cfr10I RCCGGY 1 cut(s) 560
Csp6I GTAC 2 cut(s) 553, 589
CviAII CATG 7 cut(s) 65, 89, 113, 227, 233, 287, 369
CviQI GTAC 2 cut(s) 553, 589
DpnI GATC 4 cut(s) 69, 93, 165, 189
DpnII GATC 4 cut(s) 67, 91, 163, 187
EaeI YGGCCR 1 cut(s) 415
Eco130I CCWWGG 2 cut(s) 128, 152
Eco147I AGGCCT 3 cut(s) 133, 157, 529
EcoRII CCWGG 2 cut(s) 312, 412
EcoT14I CCWWGG 2 cut(s) 128, 152
EcoT22I ATGCAT 1 cut(s) 340
ErhI CCWWGG 2 cut(s) 128, 152
FaeI CATG 7 cut(s) 68, 92, 116, 230, 236, 290, 372
FatI CATG 7 cut(s) 64, 88, 112, 226, 232, 286, 368
FbaI TGATCA 4 cut(s) 67, 91, 163, 187
Fnu4HI GCNGC 1 cut(s) 549
FokI GGATG 1 cut(s) 501
Fsp4HI GCNGC 1 cut(s) 549
GluI GCNGC 1 cut(s) 549
GsuI CTGGAG 1 cut(s) 335
HaeIII GGCC 6 cut(s) 133, 157, 312, 417, 529, 560
HapII CCGG 2 cut(s) 258, 561
Hin1II CATG 7 cut(s) 68, 92, 116, 230, 236, 290, 372
HincII GTYRAC 1 cut(s) 213
HindII GTYRAC 1 cut(s) 213
HinfI GANTC 1 cut(s) 392
HpaII CCGG 2 cut(s) 258, 561
Hpy166II GTNNAC 2 cut(s) 213, 509
Hpy188III TCNNGA 6 cut(s) 65, 89, 113, 258, 299, 538
Hpy8I GTNNAC 2 cut(s) 213, 509
HpyAV CCTTC 2 cut(s) 242, 296
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4IV ACGT 1 cut(s) 179
HpyCH4V TGCA 5 cut(s) 208, 338, 368, 408, 495
HpyF10VI GCNNNNNNNGC 4 cut(s) 205, 414, 418, 557
HpySE526I ACGT 1 cut(s) 179
Hsp92II CATG 7 cut(s) 68, 92, 116, 230, 236, 290, 372
Kpn2I TCCGGA 1 cut(s) 257
KroI GCCGGC 1 cut(s) 560
KroNI GCCGGC 1 cut(s) 562
Ksp22I TGATCA 4 cut(s) 67, 91, 163, 187
Kzo9I GATC 4 cut(s) 67, 91, 163, 187
LmnI GCTCC 1 cut(s) 272
Lsp1109I GCAGC 1 cut(s) 535
LweI GCATC 1 cut(s) 573
MaeII ACGT 1 cut(s) 179
MaeIII GTNAC 1 cut(s) 433
MalI GATC 4 cut(s) 69, 93, 165, 189
MboI GATC 4 cut(s) 67, 91, 163, 187
MboII GAAGA 1 cut(s) 28
MhlI GDGCHC 1 cut(s) 455
MlsI TGGCCA 1 cut(s) 417
MluCI AATT 2 cut(s) 58, 460
MluNI TGGCCA 1 cut(s) 417
MlyI GAGTC 1 cut(s) 386
MnlI CCTC 7 cut(s) 96, 120, 245, 382, 408, 465, 479
Mox20I TGGCCA 1 cut(s) 417
Mph1103I ATGCAT 1 cut(s) 340
MroI TCCGGA 1 cut(s) 257
MroNI GCCGGC 1 cut(s) 560
MscI TGGCCA 1 cut(s) 417
MslI CAYNNNNRTG 1 cut(s) 231
Msp20I TGGCCA 1 cut(s) 417
MspI CCGG 2 cut(s) 258, 561
MspR9I CCNGG 2 cut(s) 314, 414
MvaI CCWGG 2 cut(s) 314, 414
MwoI GCNNNNNNNGC 4 cut(s) 205, 414, 418, 557
NaeI GCCGGC 1 cut(s) 562
NdeII GATC 4 cut(s) 67, 91, 163, 187
NgoMIV GCCGGC 1 cut(s) 560
NlaIII CATG 7 cut(s) 68, 92, 116, 230, 236, 290, 372
NlaIV GGNNCC 1 cut(s) 256
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 2 cut(s) 230, 372
NspV TTCGAA 1 cut(s) 375
PaeI GCATGC 1 cut(s) 372
PagI TCATGA 3 cut(s) 64, 88, 112
PceI AGGCCT 3 cut(s) 133, 157, 529
PdiI GCCGGC 1 cut(s) 562
PkrI GCNGC 1 cut(s) 550
PleI GAGTC 1 cut(s) 386
PpsI GAGTC 1 cut(s) 386
Psp1406I AACGTT 1 cut(s) 179
Psp6I CCWGG 2 cut(s) 312, 412
PspGI CCWGG 2 cut(s) 312, 412
PspN4I GGNNCC 1 cut(s) 256
RsaI GTAC 2 cut(s) 554, 590
RsaNI GTAC 2 cut(s) 553, 589
RseI CAYNNNNRTG 1 cut(s) 231
SatI GCNGC 1 cut(s) 549
Sau3AI GATC 4 cut(s) 67, 91, 163, 187
SchI GAGTC 1 cut(s) 386
ScrFI CCNGG 2 cut(s) 314, 414
SduI GDGCHC 1 cut(s) 455
SetI ASST 6 cut(s) 16, 182, 256, 277, 307, 590
SfaNI GCATC 1 cut(s) 573
SfuI TTCGAA 1 cut(s) 375
SmiMI CAYNNNNRTG 1 cut(s) 231
SphI GCATGC 1 cut(s) 372
Sse9I AATT 2 cut(s) 58, 460
SseBI AGGCCT 3 cut(s) 133, 157, 529
SsiI CCGC 1 cut(s) 583
StuI AGGCCT 3 cut(s) 133, 157, 529
StyD4I CCNGG 2 cut(s) 312, 412
StyI CCWWGG 2 cut(s) 128, 152
TaaI ACNGT 1 cut(s) 322
TaiI ACGT 1 cut(s) 182
TaqI TCGA 3 cut(s) 375, 516, 537
TasI AATT 2 cut(s) 58, 460
TscAI CASTG 1 cut(s) 455
TseI GCWGC 1 cut(s) 548
TspDTI ATGAA 1 cut(s) 261
TspGWI ACGGA 1 cut(s) 498
TspRI CASTG 1 cut(s) 455
XceI RCATGY 2 cut(s) 230, 372
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.