Rmu_sc0003266.1_g000014

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003266.1
OG0031212
Physical Location & Seq
Reverse (-)
65415 .. 65879
465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003266.1_g000014.1.cds

Sequence Viewer

Length: 465 bp
atgacaaggcacatgaatgatgtgctccaaaatgaatacctcaatgaggaagacccaagaaagctttgggtagaacttgagcagcgatttggcaacatttgtgactccctgcttcctgatttagaagtgcgatggcatagcctccgcttttgtgatttcaagtctatgcttgattataactcggaagctcttcgtgtcaagtctctgatggagttttgtggccaagccataactaatactatgttgatcgagaagactctctccaccttccccgtctctgcactgatgatgtgggaactaagcctattccggagtctaaccatagtcgcgcccccaatggaggacgcaaggagcgaaaccctaagagtagggaaaattctggacattctggtccatatgttcgccctaaagaggaaggaaatagccgagaaaggcatgcacggaaccgtggaggtcaacgtgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 zf-CCHC

Protein Analysis

154

Amino Acids

17.94

Weight (kDa)

5.4

Isoelectric Point (pI)

52.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031212)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002519.1_g000002 Rmu_sc0003266.1_g000014

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AasI GACNNNNNNGTC 1 cut(s) 389
AccII CGCG 1 cut(s) 329
AccIII TCCGGA 1 cut(s) 309
AciI CCGC 1 cut(s) 145
AcoI YGGCCR 1 cut(s) 220
AcsI RAATTY 1 cut(s) 375
AfiI CCNNNNNNNGG 3 cut(s) 46, 340, 411
AflIII ACRYGT 1 cut(s) 459
AgsI TTSAA 1 cut(s) 160
AluBI AGCT 2 cut(s) 64, 188
AluI AGCT 2 cut(s) 64, 188
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 2 cut(s) 207, 280
Aor13HI TCCGGA 1 cut(s) 309
AoxI GGCC 1 cut(s) 220
ApeKI GCWGC 1 cut(s) 82
ApoI RAATTY 1 cut(s) 375
Asp700I GAANNNNTTC 1 cut(s) 189
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 1 cut(s) 391
AvaII GGWCC 1 cut(s) 391
BalI TGGCCA 1 cut(s) 222
BbsI GAAGAC 2 cut(s) 57, 260
Bbv12I GWGCWC 1 cut(s) 27
BbvI GCAGC 1 cut(s) 94
BccI CCATC 2 cut(s) 126, 202
BcoDI GTCTC 2 cut(s) 207, 280
BisI GCNGC 1 cut(s) 83
BlsI GCNGC 1 cut(s) 84
Bme18I GGWCC 1 cut(s) 391
BmgT120I GGNCC 1 cut(s) 391
BmiI GGNNCC 1 cut(s) 445
BpiI GAAGAC 2 cut(s) 57, 260
BpuEI CTTGAG 1 cut(s) 98
BsaJI CCNNGG 1 cut(s) 447
BsaWI WCCGGW 1 cut(s) 309
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 340, 411
BseAI TCCGGA 1 cut(s) 309
BseDI CCNNGG 1 cut(s) 447
BseLI CCNNNNNNNGG 3 cut(s) 46, 340, 411
BseXI GCAGC 1 cut(s) 94
BsgI GTGCAG 1 cut(s) 264
Bsh1236I CGCG 1 cut(s) 329
BshFI GGCC 1 cut(s) 222
BsiHKAI GWGCWC 1 cut(s) 27
BsiSI CCGG 1 cut(s) 310
BslI CCNNNNNNNGG 3 cut(s) 46, 340, 411
BsmAI GTCTC 2 cut(s) 207, 280
BsmBI CGTCTC 1 cut(s) 280
BsnI GGCC 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 27
Bsp13I TCCGGA 1 cut(s) 309
Bsp143I GATC 1 cut(s) 246
BspACI CCGC 1 cut(s) 145
BspANI GGCC 1 cut(s) 222
BspEI TCCGGA 1 cut(s) 309
BspFNI CGCG 1 cut(s) 329
BspLI GGNNCC 1 cut(s) 445
BspQI GCTCTTC 1 cut(s) 195
BssECI CCNNGG 1 cut(s) 447
BssMI GATC 1 cut(s) 246
Bst4CI ACNGT 1 cut(s) 448
Bst6I CTCTTC 1 cut(s) 195
BstC8I GCNNGC 1 cut(s) 437
BstDEI CTNAG 2 cut(s) 299, 362
BstDSI CCRYGG 1 cut(s) 447
BstENI CCTNNNNNAGG 1 cut(s) 44
BstFNI CGCG 1 cut(s) 329
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 1 cut(s) 249
BstMAI GTCTC 2 cut(s) 207, 280
BstMBI GATC 1 cut(s) 246
BstNSI RCATGY 1 cut(s) 439
BstUI CGCG 1 cut(s) 329
BstV1I GCAGC 1 cut(s) 94
BstV2I GAAGAC 2 cut(s) 57, 260
BsuRI GGCC 1 cut(s) 222
BtgI CCRYGG 1 cut(s) 447
BtgZI GCGATG 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 281
Cac8I GCNNGC 1 cut(s) 437
CfoI GCGC 1 cut(s) 331
Cfr13I GGNCC 1 cut(s) 391
CseI GACGC 1 cut(s) 353
CviAII CATG 2 cut(s) 13, 436
CviJI RGCY 7 cut(s) 64, 141, 188, 222, 227, 303, 425
CviKI_1 RGCY 7 cut(s) 64, 141, 188, 222, 227, 303, 425
DdeI CTNAG 2 cut(s) 299, 362
DpnI GATC 1 cut(s) 248
DpnII GATC 1 cut(s) 246
DrdI GACNNNNNNGTC 1 cut(s) 389
DseDI GACNNNNNNGTC 1 cut(s) 389
EaeI YGGCCR 1 cut(s) 220
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 391
EcoNI CCTNNNNNAGG 1 cut(s) 44
Esp3I CGTCTC 1 cut(s) 280
FaeI CATG 2 cut(s) 16, 439
FatI CATG 2 cut(s) 12, 435
FauNDI CATATG 1 cut(s) 396
Fnu4HI GCNGC 1 cut(s) 83
Fsp4HI GCNGC 1 cut(s) 83
GlaI GCGC 1 cut(s) 330
GluI GCNGC 1 cut(s) 83
HaeIII GGCC 1 cut(s) 222
HapII CCGG 1 cut(s) 310
HgaI GACGC 1 cut(s) 353
HhaI GCGC 1 cut(s) 331
Hin1II CATG 2 cut(s) 16, 439
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HindIII AAGCTT 1 cut(s) 62
HinfI GANTC 3 cut(s) 104, 256, 313
HpaII CCGG 1 cut(s) 310
Hpy166II GTNNAC 1 cut(s) 457
Hpy188I TCNGA 2 cut(s) 184, 207
Hpy188III TCNNGA 4 cut(s) 116, 250, 310, 380
Hpy8I GTNNAC 1 cut(s) 457
HpyAV CCTTC 2 cut(s) 277, 409
HpyCH4III ACNGT 1 cut(s) 448
HpyCH4IV ACGT 1 cut(s) 459
HpyCH4V TGCA 2 cut(s) 281, 439
HpyF3I CTNAG 2 cut(s) 299, 362
HpySE526I ACGT 1 cut(s) 459
Hsp92II CATG 2 cut(s) 16, 439
HspAI GCGC 1 cut(s) 329
Kpn2I TCCGGA 1 cut(s) 309
Kzo9I GATC 1 cut(s) 246
LguI GCTCTTC 1 cut(s) 195
LmnI GCTCC 2 cut(s) 30, 351
LpnPI CCDG 5 cut(s) 122, 129, 323, 365, 374
Lsp1109I GCAGC 1 cut(s) 94
MaeII ACGT 1 cut(s) 459
MaeIII GTNAC 1 cut(s) 101
MalI GATC 1 cut(s) 248
MboI GATC 1 cut(s) 246
MboII GAAGA 3 cut(s) 62, 182, 265
MhlI GDGCHC 1 cut(s) 27
MlsI TGGCCA 1 cut(s) 222
MluCI AATT 1 cut(s) 375
MluNI TGGCCA 1 cut(s) 222
MlyI GAGTC 3 cut(s) 98, 250, 322
MnlI CCTC 6 cut(s) 40, 50, 152, 334, 405, 445
Mox20I TGGCCA 1 cut(s) 222
MroI TCCGGA 1 cut(s) 309
MroXI GAANNNNTTC 1 cut(s) 189
MscI TGGCCA 1 cut(s) 222
MslI CAYNNNNRTG 1 cut(s) 15
Msp20I TGGCCA 1 cut(s) 222
MspI CCGG 1 cut(s) 310
MvnI CGCG 1 cut(s) 329
NdeI CATATG 1 cut(s) 396
NdeII GATC 1 cut(s) 246
NlaIII CATG 2 cut(s) 16, 439
NlaIV GGNNCC 1 cut(s) 445
NmeAIII GCCGAG 1 cut(s) 451
NmuCI GTSAC 1 cut(s) 101
NspI RCATGY 1 cut(s) 439
PaeI GCATGC 1 cut(s) 439
PciSI GCTCTTC 1 cut(s) 195
PcsI WCGNNNNNNNCGW 1 cut(s) 351
PdmI GAANNNNTTC 1 cut(s) 189
PkrI GCNGC 1 cut(s) 84
PleI GAGTC 3 cut(s) 98, 250, 321
PpsI GAGTC 3 cut(s) 98, 250, 321
PsiI TTATAA 1 cut(s) 177
PspN4I GGNNCC 1 cut(s) 445
PspPI GGNCC 1 cut(s) 391
RseI CAYNNNNRTG 1 cut(s) 15
SapI GCTCTTC 1 cut(s) 195
SatI GCNGC 1 cut(s) 83
Sau3AI GATC 1 cut(s) 246
Sau96I GGNCC 1 cut(s) 391
SchI GAGTC 3 cut(s) 98, 250, 322
SduI GDGCHC 1 cut(s) 27
SetI ASST 6 cut(s) 42, 66, 190, 269, 456, 462
SinI GGWCC 1 cut(s) 391
SmiMI CAYNNNNRTG 1 cut(s) 15
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
SphI GCATGC 1 cut(s) 439
Sse9I AATT 1 cut(s) 375
SsiI CCGC 1 cut(s) 145
TaaI ACNGT 1 cut(s) 448
TaiI ACGT 1 cut(s) 462
TaqI TCGA 1 cut(s) 249
TasI AATT 1 cut(s) 375
TscAI CASTG 1 cut(s) 288
TseFI GTSAC 1 cut(s) 101
TseI GCWGC 1 cut(s) 82
Tsp45I GTSAC 1 cut(s) 101
TspDTI ATGAA 2 cut(s) 29, 48
TspGWI ACGGA 1 cut(s) 456
TspRI CASTG 1 cut(s) 288
VpaK11BI GGWCC 1 cut(s) 391
XagI CCTNNNNNAGG 1 cut(s) 44
XapI RAATTY 1 cut(s) 375
XceI RCATGY 1 cut(s) 439
XcmI CCANNNNNNNNNTGG 1 cut(s) 63
XmnI GAANNNNTTC 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.