Rmu_sc0003291.1_g000008

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003291.1
OG0025558
Physical Location & Seq
Forward (+)
53538 .. 54401
864 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 777 bp
atggcggcgggggaagcggcgtcgacgctttccctggcggaggaggccgtggtaagcctgattggggacggagaagcactccaggatccctacttttatctctgtggccggctgctagctccaaaacgtcccgtggccttcccatcttttgcggcggcgatttcaggagtgtggcggcttaaggaaagggttttgattagtgaagagaaggtaggggtcttcgtatttcaaattaaggcactggaggagaaggaccacattcttcacggtgaactctggatagctgtgacgggactccgagtttctatgagcaatgagcgagctctaactctaatcgggaatgatctgggggccttggtgaaggtagatgcagcggcgttgaagcggaaggctcccgtccaacggatccgggttgttcatgacatgcgtttgaggatttgggcacgacgtacgtactgcttctcaccggaggtttctgttatgctagaattcaggtatgagaaatgtcatggtatatgcccagattgtgggttgtttggtcatggtggtggtgcctgcgacaaggggctgctgcagatgcttttgtcggtgtcgctggtgccggggactctgcaacaggacctgggctcggagaagccagcagaggaggtggtgatggttggacgggagagggcagatagaccggaggtggaactagcagctgctgcaagtttggaggccccgattttttcggtgggaaaccccaactgcaaattagggttgaccccaaggccatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 Exo_endo_phos_2 RNase_H RVT_1 zf-CCHC_4

Protein Analysis

258

Amino Acids

28.02

Weight (kDa)

6.54

Isoelectric Point (pI)

40.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 141 - 188 6.4e-07 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0025558)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003291.1_g000008 Rmu_sc0028489.1_g000001 Rmu_sc0038374.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 551, 598
AccB7I CCANNNNNTGG 1 cut(s) 527
AccI GTMKAC 1 cut(s) 23
AciI CCGC 9 cut(s) 5, 8, 17, 38, 152, 155, 175, 374, 385
AclWI GGATC 4 cut(s) 80, 93, 400, 413
AcoI YGGCCR 1 cut(s) 106
AcsI RAATTY 1 cut(s) 488
AcyI GRCGYC 1 cut(s) 20
AfaI GTAC 2 cut(s) 451, 455
AfiI CCNNNNNNNGG 5 cut(s) 40, 64, 402, 527, 628
AflII CTTAAG 1 cut(s) 179
AgsI TTSAA 2 cut(s) 230, 382
AjnI CCWGG 3 cut(s) 33, 81, 621
AluBI AGCT 4 cut(s) 119, 284, 323, 701
AluI AGCT 4 cut(s) 119, 284, 323, 701
Alw21I GWGCWC 1 cut(s) 325
AlwI GGATC 4 cut(s) 80, 93, 400, 413
AlwNI CAGNNNCTG 2 cut(s) 622, 704
AoxI GGCC 6 cut(s) 45, 106, 135, 351, 717, 770
ApeKI GCWGC 7 cut(s) 112, 371, 568, 571, 698, 701, 704
ApoI RAATTY 1 cut(s) 488
AspS9I GGNCC 4 cut(s) 253, 351, 619, 718
AsuC2I CCSGG 2 cut(s) 410, 603
AsuHPI GGTGA 4 cut(s) 281, 370, 456, 664
AsuNHI GCTAGC 1 cut(s) 115
AvaII GGWCC 2 cut(s) 253, 619
BaeGI GKGCMC 1 cut(s) 445
BamHI GGATCC 2 cut(s) 85, 405
BanI GGYRCC 2 cut(s) 551, 598
BanII GRGCYC 2 cut(s) 325, 629
BbsI GAAGAC 1 cut(s) 211
Bbv12I GWGCWC 1 cut(s) 325
BbvI GCAGC 7 cut(s) 99, 383, 555, 558, 688, 691, 710
BccI CCATC 2 cut(s) 151, 649
BceAI ACGGC 1 cut(s) 32
BciT130I CCWGG 3 cut(s) 35, 83, 623
BcnI CCSGG 2 cut(s) 410, 603
BfaI CTAG 3 cut(s) 116, 485, 695
BfmI CTRYAG 1 cut(s) 572
BfrI CTTAAG 1 cut(s) 179
Bme1390I CCNGG 5 cut(s) 35, 83, 410, 603, 623
Bme18I GGWCC 2 cut(s) 253, 619
BmgT120I GGNCC 4 cut(s) 253, 351, 619, 718
BmiI GGNNCC 7 cut(s) 87, 352, 393, 407, 553, 600, 720
BmrFI CCNGG 5 cut(s) 35, 83, 410, 603, 623
BmsI GCATC 2 cut(s) 358, 567
BmtI GCTAGC 1 cut(s) 119
BpiI GAAGAC 1 cut(s) 211
BplI GAGNNNNNCTC 2 cut(s) 63, 95
BpmI CTGGAG 2 cut(s) 65, 263
BpuMI CCSGG 2 cut(s) 410, 603
BsaAI YACGTR 1 cut(s) 453
BsaHI GRCGYC 1 cut(s) 20
BsaJI CCNNGG 7 cut(s) 33, 48, 132, 354, 602, 622, 767
BsaWI WCCGGW 2 cut(s) 466, 682
Bsc4I CCNNNNNNNGG 5 cut(s) 40, 64, 402, 527, 628
Bse118I RCCGGY 1 cut(s) 108
Bse1I ACTGG 1 cut(s) 246
Bse3DI GCAATG 1 cut(s) 319
BseBI CCWGG 3 cut(s) 35, 83, 623
BseDI CCNNGG 7 cut(s) 33, 48, 132, 354, 602, 622, 767
BseLI CCNNNNNNNGG 5 cut(s) 40, 64, 402, 527, 628
BseMI GCAATG 1 cut(s) 319
BseNI ACTGG 1 cut(s) 246
BseRI GAGGAG 3 cut(s) 56, 260, 659
BseSI GKGCMC 1 cut(s) 445
BseXI GCAGC 7 cut(s) 99, 383, 555, 558, 688, 691, 710
BshFI GGCC 6 cut(s) 47, 108, 137, 353, 719, 772
BshNI GGYRCC 2 cut(s) 551, 598
BsiHKAI GWGCWC 1 cut(s) 325
BsiSI CCGG 5 cut(s) 109, 409, 467, 602, 683
BsiWI CGTACG 1 cut(s) 449
BslFI GGGAC 4 cut(s) 80, 114, 306, 619
BslI CCNNNNNNNGG 5 cut(s) 40, 64, 402, 527, 628
BsmFI GGGAC 4 cut(s) 80, 114, 306, 619
BsnI GGCC 6 cut(s) 47, 108, 137, 353, 719, 772
Bsp1286I GDGCHC 3 cut(s) 325, 445, 629
Bsp143I GATC 3 cut(s) 85, 343, 405
BspACI CCGC 9 cut(s) 5, 8, 17, 38, 152, 155, 175, 374, 385
BspANI GGCC 6 cut(s) 47, 108, 137, 353, 719, 772
BspHI TCATGA 1 cut(s) 418
BspLI GGNNCC 7 cut(s) 87, 352, 393, 407, 553, 600, 720
BspMAI CTGCAG 1 cut(s) 576
BspOI GCTAGC 1 cut(s) 119
BspPI GGATC 4 cut(s) 80, 93, 400, 413
BspT107I GGYRCC 2 cut(s) 551, 598
BspTI CTTAAG 1 cut(s) 179
BsrDI GCAATG 1 cut(s) 319
BsrFI RCCGGY 1 cut(s) 108
BsrI ACTGG 1 cut(s) 246
BssAI RCCGGY 1 cut(s) 108
BssECI CCNNGG 7 cut(s) 33, 48, 132, 354, 602, 622, 767
BssMI GATC 3 cut(s) 85, 343, 405
BssNI GRCGYC 1 cut(s) 20
BssT1I CCWWGG 2 cut(s) 354, 767
Bst2UI CCWGG 3 cut(s) 35, 83, 623
Bst4CI ACNGT 1 cut(s) 269
Bst6I CTCTTC 1 cut(s) 198
BstACI GRCGYC 1 cut(s) 20
BstAFI CTTAAG 1 cut(s) 179
BstAPI GCANNNNNTGC 1 cut(s) 704
BstBAI YACGTR 1 cut(s) 453
BstC8I GCNNGC 5 cut(s) 110, 117, 321, 556, 639
BstDSI CCRYGG 2 cut(s) 48, 132
BstENI CCTNNNNNAGG 1 cut(s) 38
BstKTI GATC 3 cut(s) 88, 346, 408
BstMBI GATC 3 cut(s) 85, 343, 405
BstMWI GCNNNNNNNGC 4 cut(s) 14, 44, 577, 704
BstNI CCWGG 3 cut(s) 35, 83, 623
BstNSI RCATGY 1 cut(s) 427
BstSCI CCNGG 5 cut(s) 33, 81, 408, 601, 621
BstSFI CTRYAG 1 cut(s) 572
BstSLI GKGCMC 1 cut(s) 445
BstSNI TACGTA 1 cut(s) 453
BstV1I GCAGC 7 cut(s) 99, 383, 555, 558, 688, 691, 710
BstV2I GAAGAC 1 cut(s) 211
BstX2I RGATCY 2 cut(s) 85, 405
BstYI RGATCY 2 cut(s) 85, 405
BsuRI GGCC 6 cut(s) 47, 108, 137, 353, 719, 772
BtgI CCRYGG 2 cut(s) 48, 132
BtsIMutI CAGTG 1 cut(s) 239
Cac8I GCNNGC 5 cut(s) 110, 117, 321, 556, 639
CaiI CAGNNNCTG 2 cut(s) 622, 704
CciI TCATGA 1 cut(s) 418
Cfr10I RCCGGY 1 cut(s) 108
Cfr13I GGNCC 4 cut(s) 253, 351, 619, 718
CseI GACGC 2 cut(s) 9, 34
Csp6I GTAC 2 cut(s) 450, 454
CviAII CATG 4 cut(s) 419, 424, 509, 542
CviQI GTAC 2 cut(s) 450, 454
DpnI GATC 3 cut(s) 87, 345, 407
DpnII GATC 3 cut(s) 85, 343, 405
EaeI YGGCCR 1 cut(s) 106
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
EciI GGCGGA 1 cut(s) 53
Ecl136II GAGCTC 1 cut(s) 323
Eco105I TACGTA 1 cut(s) 453
Eco130I CCWWGG 2 cut(s) 354, 767
Eco24I GRGCYC 2 cut(s) 325, 629
Eco47I GGWCC 2 cut(s) 253, 619
Eco53kI GAGCTC 1 cut(s) 323
EcoICRI GAGCTC 1 cut(s) 323
EcoNI CCTNNNNNAGG 1 cut(s) 38
EcoO109I RGGNCCY 3 cut(s) 351, 619, 718
EcoRI GAATTC 1 cut(s) 488
EcoRII CCWGG 3 cut(s) 33, 81, 621
EcoT14I CCWWGG 2 cut(s) 354, 767
EcoT38I GRGCYC 2 cut(s) 325, 629
ErhI CCWWGG 2 cut(s) 354, 767
FaeI CATG 4 cut(s) 422, 427, 512, 545
FaqI GGGAC 4 cut(s) 80, 114, 306, 619
FatI CATG 4 cut(s) 418, 423, 508, 541
FblI GTMKAC 1 cut(s) 23
FriOI GRGCYC 2 cut(s) 325, 629
FspBI CTAG 3 cut(s) 116, 485, 695
GsuI CTGGAG 2 cut(s) 65, 263
HaeIII GGCC 6 cut(s) 47, 108, 137, 353, 719, 772
HapII CCGG 5 cut(s) 109, 409, 467, 602, 683
HgaI GACGC 2 cut(s) 9, 34
Hin1I GRCGYC 1 cut(s) 20
Hin1II CATG 4 cut(s) 422, 427, 512, 545
HincII GTYRAC 2 cut(s) 24, 762
HindII GTYRAC 2 cut(s) 24, 762
HinfI GANTC 2 cut(s) 294, 607
HpaII CCGG 5 cut(s) 109, 409, 467, 602, 683
HphI GGTGA 4 cut(s) 281, 370, 456, 664
Hpy166II GTNNAC 3 cut(s) 24, 272, 762
Hpy188I TCNGA 2 cut(s) 299, 631
Hpy188III TCNNGA 4 cut(s) 165, 277, 337, 419
Hpy8I GTNNAC 3 cut(s) 24, 272, 762
Hpy99I CGWCG 3 cut(s) 25, 28, 450
HpyAV CCTTC 5 cut(s) 148, 202, 244, 355, 382
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4IV ACGT 3 cut(s) 127, 448, 452
HpyCH4V TGCA 5 cut(s) 371, 574, 613, 707, 750
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 44, 577, 704
HpySE526I ACGT 3 cut(s) 127, 448, 452
Hsp92I GRCGYC 1 cut(s) 20
Hsp92II CATG 4 cut(s) 422, 427, 512, 545
KroI GCCGGC 1 cut(s) 108
KroNI GCCGGC 1 cut(s) 110
Kzo9I GATC 3 cut(s) 85, 343, 405
LmnI GCTCC 2 cut(s) 124, 397
Lsp1109I GCAGC 7 cut(s) 99, 383, 555, 558, 688, 691, 710
LweI GCATC 2 cut(s) 358, 567
MaeI CTAG 3 cut(s) 116, 485, 695
MaeII ACGT 3 cut(s) 127, 448, 452
MaeIII GTNAC 1 cut(s) 286
MalI GATC 3 cut(s) 87, 345, 407
MboI GATC 3 cut(s) 85, 343, 405
MboII GAAGA 3 cut(s) 211, 215, 254
MflI RGATCY 2 cut(s) 85, 405
MhlI GDGCHC 3 cut(s) 325, 445, 629
MluCI AATT 3 cut(s) 231, 488, 752
MlyI GAGTC 2 cut(s) 288, 601
MmeI TCCRAC 2 cut(s) 424, 640
MroNI GCCGGC 1 cut(s) 108
MseI TTAA 2 cut(s) 180, 234
MslI CAYNNNNRTG 1 cut(s) 546
MspA1I CMGCKG 2 cut(s) 374, 701
MspCI CTTAAG 1 cut(s) 179
MspI CCGG 5 cut(s) 109, 409, 467, 602, 683
MspR9I CCNGG 5 cut(s) 35, 83, 410, 603, 623
MvaI CCWGG 3 cut(s) 35, 83, 623
MwoI GCNNNNNNNGC 4 cut(s) 14, 44, 577, 704
NaeI GCCGGC 1 cut(s) 110
NciI CCSGG 2 cut(s) 410, 603
NdeII GATC 3 cut(s) 85, 343, 405
NgoMIV GCCGGC 1 cut(s) 108
NheI GCTAGC 1 cut(s) 115
NlaIII CATG 4 cut(s) 422, 427, 512, 545
NlaIV GGNNCC 7 cut(s) 87, 352, 393, 407, 553, 600, 720
NmuCI GTSAC 1 cut(s) 286
NspI RCATGY 1 cut(s) 427
PagI TCATGA 1 cut(s) 418
PdiI GCCGGC 1 cut(s) 110
Pfl23II CGTACG 1 cut(s) 449
PflMI CCANNNNNTGG 1 cut(s) 527
PfoI TCCNGGA 1 cut(s) 81
PleI GAGTC 2 cut(s) 288, 601
PpsI GAGTC 2 cut(s) 288, 601
Ppu21I YACGTR 1 cut(s) 453
PpuMI RGGWCCY 1 cut(s) 619
Psp124BI GAGCTC 1 cut(s) 325
Psp5II RGGWCCY 1 cut(s) 619
Psp6I CCWGG 3 cut(s) 33, 81, 621
PspGI CCWGG 3 cut(s) 33, 81, 621
PspLI CGTACG 1 cut(s) 449
PspN4I GGNNCC 7 cut(s) 87, 352, 393, 407, 553, 600, 720
PspPI GGNCC 4 cut(s) 253, 351, 619, 718
PspPPI RGGWCCY 1 cut(s) 619
PstI CTGCAG 1 cut(s) 576
PstNI CAGNNNCTG 2 cut(s) 622, 704
PsuI RGATCY 2 cut(s) 85, 405
PvuII CAGCTG 1 cut(s) 701
RsaI GTAC 2 cut(s) 451, 455
RsaNI GTAC 2 cut(s) 450, 454
RseI CAYNNNNRTG 1 cut(s) 546
SacI GAGCTC 1 cut(s) 325
SalI GTCGAC 1 cut(s) 22
SaqAI TTAA 2 cut(s) 180, 234
Sau3AI GATC 3 cut(s) 85, 343, 405
Sau96I GGNCC 4 cut(s) 253, 351, 619, 718
SchI GAGTC 2 cut(s) 288, 601
ScrFI CCNGG 5 cut(s) 35, 83, 410, 603, 623
SduI GDGCHC 3 cut(s) 325, 445, 629
SfaNI GCATC 2 cut(s) 358, 567
SfcI CTRYAG 1 cut(s) 572
SgrDI CGTCGACG 1 cut(s) 22
SinI GGWCC 2 cut(s) 253, 619
SmiMI CAYNNNNRTG 1 cut(s) 546
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
SnaBI TACGTA 1 cut(s) 453
Sse9I AATT 3 cut(s) 231, 488, 752
SsiI CCGC 9 cut(s) 5, 8, 17, 38, 152, 155, 175, 374, 385
SspMI CTAG 3 cut(s) 116, 485, 695
SstI GAGCTC 1 cut(s) 325
StyD4I CCNGG 5 cut(s) 33, 81, 408, 601, 621
StyI CCWWGG 2 cut(s) 354, 767
TaaI ACNGT 1 cut(s) 269
TaiI ACGT 3 cut(s) 130, 451, 455
TaqI TCGA 1 cut(s) 23
TasI AATT 3 cut(s) 231, 488, 752
TauI GCSGC 6 cut(s) 8, 20, 155, 158, 178, 377
Tru1I TTAA 2 cut(s) 180, 234
Tru9I TTAA 2 cut(s) 180, 234
TscAI CASTG 1 cut(s) 246
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 7 cut(s) 112, 371, 568, 571, 698, 701, 704
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 1 cut(s) 407
TspGWI ACGGA 2 cut(s) 84, 418
TspRI CASTG 1 cut(s) 246
Van91I CCANNNNNTGG 1 cut(s) 527
Vha464I CTTAAG 1 cut(s) 179
VpaK11BI GGWCC 2 cut(s) 253, 619
XagI CCTNNNNNAGG 1 cut(s) 38
XapI RAATTY 1 cut(s) 488
XceI RCATGY 1 cut(s) 427
XmiI GTMKAC 1 cut(s) 23
XspI CTAG 3 cut(s) 116, 485, 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.