Rmu_sc0028489.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0028489.1
OG0025558
Physical Location & Seq
Forward (+)
1363 .. 2484
1122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0028489.1_g000001.1.cds

Sequence Viewer

Length: 969 bp
atggcagcatcgtccagactgtcgctgttggacggagtggaggaggtcgtgagcctggtcgggggcagcgaagcaattcatgatcccttcttttatctgtgcggccagatactggctccaaagaaacctgttaagatccctttgttctcggcagcaatctttggaatatggggcttaaggagaggggcagttcgatgctggtgctggcggcatatgactgaattggcttgtttggcggaggtgcctttgcatgctttggaaatctgggtggcggtgaagggtttatggtttgctatgcggaacgagcgagccctaactctattggggaatgctctgggtatctttgtttgggtagaccaagcggctgtgcagcctaaggacccggtccaaagaatctgtgtgatccaagacgtgggcctgagaatttgggcccggcgtgtgtatagcttcttgctggaatttcggtatgaaaaatgccatgggatttgcccggattgcaggttgtttggtcatggaggcggagtctgtgataagggattggtttctccagcgttacctcattcgaatccggaaccagtggatggttctctgggctcagtggcggtgacaggggagttggaatcacttggggagaaggtggtgcaacccgggttggtgcagtctgggtcggtccagttgggatcggcgcgggagatgcaggtcaacacggcggcagttctagggaaggagaccccgagtttttcggcgggaaactctaggtttaaactagagttgactgcaggggcctcatcaaaaagcccgcggatcaagtggaccgatggaggcccgccgacccggagggccaaaagcccggcccagcccgtcaaaaagcctgtaaaagcccgccttggtgggctgagggctggccaatcaaaaagccagcaaaagccctgcccggcccaccaaaagcctgctaggcccggcccgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

322

Amino Acids

34.69

Weight (kDa)

9.58

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025558)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003291.1_g000008 Rmu_sc0028489.1_g000001 Rmu_sc0038374.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 489, 688
AccB1I GGYRCC 1 cut(s) 241
AccB7I CCANNNNNTGG 3 cut(s) 112, 412, 581
AccI GTMKAC 1 cut(s) 354
AccII CGCG 2 cut(s) 688, 802
AccIII TCCGGA 1 cut(s) 568
AclWI GGATC 5 cut(s) 77, 130, 397, 688, 812
AcoI YGGCCR 2 cut(s) 103, 904
AcsI RAATTY 2 cut(s) 423, 458
AfiI CCNNNNNNNGG 4 cut(s) 61, 112, 412, 581
AflII CTTAAG 1 cut(s) 175
AjiI CACGTC 1 cut(s) 412
AjnI CCWGG 1 cut(s) 54
AluBI AGCT 1 cut(s) 447
AluI AGCT 1 cut(s) 447
Alw26I GTCTC 1 cut(s) 722
AlwI GGATC 5 cut(s) 77, 130, 397, 688, 812
AlwNI CAGNNNCTG 1 cut(s) 112
Ama87I CYCGRG 2 cut(s) 647, 733
Aor13HI TCCGGA 1 cut(s) 568
ApaI GGGCCC 1 cut(s) 433
ApeKI GCWGC 4 cut(s) 5, 66, 152, 370
ApoI RAATTY 2 cut(s) 423, 458
Asp700I GAANNNNTTC 1 cut(s) 75
AspLEI GCGC 1 cut(s) 688
AsuC2I CCSGG 9 cut(s) 383, 433, 491, 648, 649, 835, 851, 935, 960
AsuHPI GGTGA 2 cut(s) 286, 616
AsuII TTCGAA 1 cut(s) 563
AvaI CYCGRG 2 cut(s) 647, 733
AvaII GGWCC 4 cut(s) 379, 385, 670, 813
AxyI CCTNAGG 1 cut(s) 375
BaeGI GKGCMC 1 cut(s) 433
BalI TGGCCA 1 cut(s) 906
BanI GGYRCC 1 cut(s) 241
BanII GRGCYC 3 cut(s) 313, 433, 596
BbvCI CCTCAGC 1 cut(s) 896
BbvI GCAGC 4 cut(s) 17, 78, 164, 382
BccI CCATC 2 cut(s) 575, 812
BceAI ACGGC 1 cut(s) 723
BcgI CGANNNNNNTGC 2 cut(s) 183, 217
BciT130I CCWGG 1 cut(s) 56
BcnI CCSGG 9 cut(s) 383, 433, 491, 648, 649, 835, 851, 935, 960
BcoDI GTCTC 1 cut(s) 722
BfaI CTAG 4 cut(s) 719, 756, 767, 954
BfmI CTRYAG 1 cut(s) 777
BfrI CTTAAG 1 cut(s) 175
BfuAI ACCTGC 2 cut(s) 489, 688
BglI GCCNNNNNGGC 1 cut(s) 955
BisI GCNGC 8 cut(s) 6, 67, 103, 153, 209, 363, 371, 711
BlsI GCNGC 8 cut(s) 7, 68, 104, 154, 210, 364, 372, 712
Bme18I GGWCC 4 cut(s) 379, 385, 670, 813
BmeT110I CYCGRG 2 cut(s) 647, 733
BmgBI CACGTC 1 cut(s) 412
BmiI GGNNCC 6 cut(s) 117, 243, 381, 431, 573, 784
BmsI GCATC 3 cut(s) 17, 185, 684
BpmI CTGGAG 1 cut(s) 531
Bpu10I CCTNAGC 1 cut(s) 896
Bpu14I TTCGAA 1 cut(s) 563
BpuMI CCSGG 9 cut(s) 383, 433, 491, 648, 649, 835, 851, 935, 960
BsaI GGTCTC 1 cut(s) 722
BsaJI CCNNGG 4 cut(s) 478, 647, 800, 886
BsaWI WCCGGW 1 cut(s) 568
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 4 cut(s) 61, 112, 412, 581
Bse1I ACTGG 3 cut(s) 117, 575, 673
Bse21I CCTNAGG 1 cut(s) 375
BseAI TCCGGA 1 cut(s) 568
BseBI CCWGG 1 cut(s) 56
BseDI CCNNGG 4 cut(s) 478, 647, 800, 886
BseGI GGATG 1 cut(s) 586
BseLI CCNNNNNNNGG 4 cut(s) 61, 112, 412, 581
BseMII CTCAG 3 cut(s) 410, 609, 887
BseNI ACTGG 3 cut(s) 117, 575, 673
BseRI GAGGAG 1 cut(s) 56
BseSI GKGCMC 1 cut(s) 433
BseXI GCAGC 4 cut(s) 17, 78, 164, 382
BseYI CCCAGC 1 cut(s) 855
BsgI GTGCAG 2 cut(s) 389, 677
Bsh1236I CGCG 2 cut(s) 688, 802
BshNI GGYRCC 1 cut(s) 241
BsiHKCI CYCGRG 2 cut(s) 647, 733
BsiSI CCGG 9 cut(s) 383, 433, 491, 569, 648, 835, 851, 935, 960
BslI CCNNNNNNNGG 4 cut(s) 61, 112, 412, 581
BsmAI GTCTC 1 cut(s) 722
BsmI GAATGC 1 cut(s) 334
Bso31I GGTCTC 1 cut(s) 722
BsoBI CYCGRG 2 cut(s) 647, 733
Bsp119I TTCGAA 1 cut(s) 563
Bsp120I GGGCCC 1 cut(s) 429
Bsp1286I GDGCHC 3 cut(s) 313, 433, 596
Bsp13I TCCGGA 1 cut(s) 568
Bsp143I GATC 5 cut(s) 82, 135, 402, 680, 804
Bsp19I CCATGG 1 cut(s) 478
BspCNI CTCAG 3 cut(s) 411, 608, 888
BspEI TCCGGA 1 cut(s) 568
BspFNI CGCG 2 cut(s) 688, 802
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 6 cut(s) 117, 243, 381, 431, 573, 784
BspMAI CTGCAG 1 cut(s) 781
BspMI ACCTGC 2 cut(s) 489, 688
BspPI GGATC 5 cut(s) 77, 130, 397, 688, 812
BspT104I TTCGAA 1 cut(s) 563
BspT107I GGYRCC 1 cut(s) 241
BspTI CTTAAG 1 cut(s) 175
BspTNI GGTCTC 1 cut(s) 722
BsrI ACTGG 3 cut(s) 117, 575, 673
BssECI CCNNGG 4 cut(s) 478, 647, 800, 886
BssMI GATC 5 cut(s) 82, 135, 402, 680, 804
BssT1I CCWWGG 2 cut(s) 478, 886
Bst2UI CCWGG 1 cut(s) 56
Bst4CI ACNGT 1 cut(s) 21
BstAFI CTTAAG 1 cut(s) 175
BstBI TTCGAA 1 cut(s) 563
BstC8I GCNNGC 9 cut(s) 206, 252, 309, 800, 827, 883, 904, 920, 951
BstDEI CTNAG 4 cut(s) 375, 419, 595, 896
BstDSI CCRYGG 2 cut(s) 478, 800
BstF5I GGATG 1 cut(s) 586
BstFNI CGCG 2 cut(s) 688, 802
BstHHI GCGC 1 cut(s) 688
BstKTI GATC 5 cut(s) 85, 138, 405, 683, 807
BstMAI GTCTC 1 cut(s) 722
BstMBI GATC 5 cut(s) 82, 135, 402, 680, 804
BstMWI GCNNNNNNNGC 5 cut(s) 233, 304, 495, 694, 955
BstNI CCWGG 1 cut(s) 56
BstNSI RCATGY 1 cut(s) 254
BstSFI CTRYAG 1 cut(s) 777
BstSLI GKGCMC 1 cut(s) 433
BstUI CGCG 2 cut(s) 688, 802
BstV1I GCAGC 4 cut(s) 17, 78, 164, 382
BstX2I RGATCY 1 cut(s) 135
BstYI RGATCY 1 cut(s) 135
Bsu36I CCTNAGG 1 cut(s) 375
BtgI CCRYGG 2 cut(s) 478, 800
BtrI CACGTC 1 cut(s) 412
BtsCI GGATG 1 cut(s) 586
BtsIMutI CAGTG 2 cut(s) 582, 603
BveI ACCTGC 2 cut(s) 489, 688
Cac8I GCNNGC 9 cut(s) 206, 252, 309, 800, 827, 883, 904, 920, 951
CaiI CAGNNNCTG 1 cut(s) 112
CciI TCATGA 1 cut(s) 79
CfoI GCGC 1 cut(s) 688
Cfr42I CCGCGG 1 cut(s) 803
Cfr9I CCCGGG 1 cut(s) 647
CviAII CATG 4 cut(s) 80, 251, 479, 512
DdeI CTNAG 4 cut(s) 375, 419, 595, 896
DpnI GATC 5 cut(s) 84, 137, 404, 682, 806
DpnII GATC 5 cut(s) 82, 135, 402, 680, 804
DraI TTTAAA 1 cut(s) 763
EaeI YGGCCR 2 cut(s) 103, 904
EciI GGCGGA 2 cut(s) 251, 534
Eco130I CCWWGG 2 cut(s) 478, 886
Eco24I GRGCYC 3 cut(s) 313, 433, 596
Eco31I GGTCTC 1 cut(s) 722
Eco47I GGWCC 4 cut(s) 379, 385, 670, 813
Eco81I CCTNAGG 1 cut(s) 375
Eco88I CYCGRG 2 cut(s) 647, 733
EcoO109I RGGNCCY 2 cut(s) 379, 783
EcoRII CCWGG 1 cut(s) 54
EcoT14I CCWWGG 2 cut(s) 478, 886
EcoT38I GRGCYC 3 cut(s) 313, 433, 596
ErhI CCWWGG 2 cut(s) 478, 886
FaeI CATG 4 cut(s) 83, 254, 482, 515
FalI AAGNNNNNCTT 2 cut(s) 870, 902
FatI CATG 4 cut(s) 79, 250, 478, 511
FauI CCCGC 5 cut(s) 681, 739, 807, 834, 890
FauNDI CATATG 1 cut(s) 213
FblI GTMKAC 1 cut(s) 354
Fnu4HI GCNGC 8 cut(s) 6, 67, 103, 153, 209, 363, 371, 711
FokI GGATG 1 cut(s) 593
FriOI GRGCYC 3 cut(s) 313, 433, 596
Fsp4HI GCNGC 8 cut(s) 6, 67, 103, 153, 209, 363, 371, 711
FspBI CTAG 4 cut(s) 719, 756, 767, 954
GlaI GCGC 1 cut(s) 687
GluI GCNGC 8 cut(s) 6, 67, 103, 153, 209, 363, 371, 711
GsaI CCCAGC 1 cut(s) 859
GsuI CTGGAG 1 cut(s) 531
HapII CCGG 9 cut(s) 383, 433, 491, 569, 648, 835, 851, 935, 960
HhaI GCGC 1 cut(s) 688
Hin1II CATG 4 cut(s) 83, 254, 482, 515
Hin6I GCGC 1 cut(s) 686
HinP1I GCGC 1 cut(s) 686
HincII GTYRAC 2 cut(s) 703, 774
HindII GTYRAC 2 cut(s) 703, 774
HinfI GANTC 4 cut(s) 393, 522, 565, 620
HpaII CCGG 9 cut(s) 383, 433, 491, 569, 648, 835, 851, 935, 960
HphI GGTGA 2 cut(s) 286, 616
Hpy166II GTNNAC 4 cut(s) 355, 703, 774, 813
Hpy188III TCNNGA 4 cut(s) 15, 49, 80, 569
Hpy8I GTNNAC 4 cut(s) 355, 703, 774, 813
HpyAV CCTTC 4 cut(s) 97, 271, 628, 718
HpyCH4III ACNGT 1 cut(s) 21
HpyCH4IV ACGT 1 cut(s) 411
HpyCH4V TGCA 7 cut(s) 250, 370, 498, 643, 658, 697, 779
HpyF10VI GCNNNNNNNGC 5 cut(s) 233, 304, 495, 694, 955
HpyF3I CTNAG 4 cut(s) 375, 419, 595, 896
HpySE526I ACGT 1 cut(s) 411
Hsp92II CATG 4 cut(s) 83, 254, 482, 515
HspAI GCGC 1 cut(s) 686
Kpn2I TCCGGA 1 cut(s) 568
KspI CCGCGG 1 cut(s) 803
Kzo9I GATC 5 cut(s) 82, 135, 402, 680, 804
LmnI GCTCC 1 cut(s) 121
Lsp1109I GCAGC 4 cut(s) 17, 78, 164, 382
LweI GCATC 3 cut(s) 17, 185, 684
MaeI CTAG 4 cut(s) 719, 756, 767, 954
MaeII ACGT 1 cut(s) 411
MaeIII GTNAC 2 cut(s) 552, 604
MalI GATC 5 cut(s) 84, 137, 404, 682, 806
MboI GATC 5 cut(s) 82, 135, 402, 680, 804
MflI RGATCY 1 cut(s) 135
MhlI GDGCHC 3 cut(s) 313, 433, 596
MlsI TGGCCA 1 cut(s) 906
MluCI AATT 4 cut(s) 75, 221, 423, 458
MluNI TGGCCA 1 cut(s) 906
MlyI GAGTC 1 cut(s) 531
MmeI TCCRAC 2 cut(s) 9, 597
Mox20I TGGCCA 1 cut(s) 906
MroI TCCGGA 1 cut(s) 568
MroXI GAANNNNTTC 1 cut(s) 75
MscI TGGCCA 1 cut(s) 906
MseI TTAA 3 cut(s) 132, 176, 762
Msp20I TGGCCA 1 cut(s) 906
MspA1I CMGCKG 1 cut(s) 802
MspCI CTTAAG 1 cut(s) 175
MspI CCGG 9 cut(s) 383, 433, 491, 569, 648, 835, 851, 935, 960
MssI GTTTAAAC 1 cut(s) 763
Mva1269I GAATGC 1 cut(s) 334
MvaI CCWGG 1 cut(s) 56
MvnI CGCG 2 cut(s) 688, 802
MwoI GCNNNNNNNGC 5 cut(s) 233, 304, 495, 694, 955
NciI CCSGG 9 cut(s) 383, 433, 491, 648, 649, 835, 851, 935, 960
NcoI CCATGG 1 cut(s) 478
NdeI CATATG 1 cut(s) 213
NdeII GATC 5 cut(s) 82, 135, 402, 680, 804
NlaIII CATG 4 cut(s) 83, 254, 482, 515
NlaIV GGNNCC 6 cut(s) 117, 243, 381, 431, 573, 784
NmeAIII GCCGAG 1 cut(s) 128
NmuCI GTSAC 1 cut(s) 604
NspI RCATGY 1 cut(s) 254
NspV TTCGAA 1 cut(s) 563
PaeI GCATGC 1 cut(s) 254
PagI TCATGA 1 cut(s) 79
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 1 cut(s) 334
PdmI GAANNNNTTC 1 cut(s) 75
PfeI GAWTC 3 cut(s) 393, 565, 620
PflFI GACNNNGTC 1 cut(s) 383
PflMI CCANNNNNTGG 3 cut(s) 112, 412, 581
PkrI GCNGC 8 cut(s) 7, 68, 104, 154, 210, 364, 372, 712
PleI GAGTC 1 cut(s) 530
PmeI GTTTAAAC 1 cut(s) 763
PpsI GAGTC 1 cut(s) 530
PpuMI RGGWCCY 1 cut(s) 379
Psp5II RGGWCCY 1 cut(s) 379
Psp6I CCWGG 1 cut(s) 54
PspFI CCCAGC 1 cut(s) 855
PspGI CCWGG 1 cut(s) 54
PspN4I GGNNCC 6 cut(s) 117, 243, 381, 431, 573, 784
PspOMI GGGCCC 1 cut(s) 429
PspPPI RGGWCCY 1 cut(s) 379
PstI CTGCAG 1 cut(s) 781
PstNI CAGNNNCTG 1 cut(s) 112
PsuI RGATCY 1 cut(s) 135
PsyI GACNNNGTC 1 cut(s) 383
SacII CCGCGG 1 cut(s) 803
SaqAI TTAA 3 cut(s) 132, 176, 762
SatI GCNGC 8 cut(s) 6, 67, 103, 153, 209, 363, 371, 711
Sau3AI GATC 5 cut(s) 82, 135, 402, 680, 804
SchI GAGTC 1 cut(s) 531
SduI GDGCHC 3 cut(s) 313, 433, 596
SfaNI GCATC 3 cut(s) 17, 185, 684
SfcI CTRYAG 1 cut(s) 777
Sfr303I CCGCGG 1 cut(s) 803
SfuI TTCGAA 1 cut(s) 563
SgrBI CCGCGG 1 cut(s) 803
SinI GGWCC 4 cut(s) 379, 385, 670, 813
SmaI CCCGGG 1 cut(s) 649
SmlI CTYRAG 1 cut(s) 175
SmoI CTYRAG 1 cut(s) 175
SphI GCATGC 1 cut(s) 254
Sse9I AATT 4 cut(s) 75, 221, 423, 458
SspMI CTAG 4 cut(s) 719, 756, 767, 954
StyI CCWWGG 2 cut(s) 478, 886
TaaI ACNGT 1 cut(s) 21
TaiI ACGT 1 cut(s) 414
TaqI TCGA 2 cut(s) 193, 563
TaqII GACCGA 2 cut(s) 658, 830
TasI AATT 4 cut(s) 75, 221, 423, 458
TauI GCSGC 4 cut(s) 105, 211, 365, 713
TfiI GAWTC 3 cut(s) 393, 565, 620
Tru1I TTAA 3 cut(s) 132, 176, 762
Tru9I TTAA 3 cut(s) 132, 176, 762
TscAI CASTG 2 cut(s) 582, 603
TseFI GTSAC 1 cut(s) 604
TseI GCWGC 4 cut(s) 5, 66, 152, 370
Tsp45I GTSAC 1 cut(s) 604
TspDTI ATGAA 2 cut(s) 68, 483
TspGWI ACGGA 1 cut(s) 48
TspMI CCCGGG 1 cut(s) 647
TspRI CASTG 2 cut(s) 582, 603
Tth111I GACNNNGTC 1 cut(s) 383
Van91I CCANNNNNTGG 3 cut(s) 112, 412, 581
Vha464I CTTAAG 1 cut(s) 175
VpaK11BI GGWCC 4 cut(s) 379, 385, 670, 813
XapI RAATTY 2 cut(s) 423, 458
XceI RCATGY 1 cut(s) 254
XmaI CCCGGG 1 cut(s) 647
XmiI GTMKAC 1 cut(s) 354
XmnI GAANNNNTTC 1 cut(s) 75
XspI CTAG 4 cut(s) 719, 756, 767, 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.