Belongs to the cystatin family. Phytocystatin subfamily
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| Species | Orthologous Gene IDs |
|---|---|
| rosa_chinensis | RchiOBHm_Chr2g0140821 |
| rosa_multiflora | Rmu_sc0003342.1_g000040 |
| rosa_rugosa | Rorug02G0365400 |
| rosa_samantha | Rh2AG416700 Rh2BG426300 Rh2CG402900 Rh2DG436200 |
| rosa_wichuraiana | Rw2G034090 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 243 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 128, 257 |
| AccI | GTMKAC | 2 cut(s) | 63, 162 |
| AciI | CCGC | 1 cut(s) | 152 |
| AclWI | GGATC | 1 cut(s) | 331 |
| AdeI | CACNNNGTG | 1 cut(s) | 92 |
| AgsI | TTSAA | 2 cut(s) | 109, 227 |
| AluBI | AGCT | 2 cut(s) | 56, 305 |
| AluI | AGCT | 2 cut(s) | 56, 305 |
| Alw26I | GTCTC | 1 cut(s) | 349 |
| AlwI | GGATC | 1 cut(s) | 331 |
| ApeKI | GCWGC | 1 cut(s) | 56 |
| AsuHPI | GGTGA | 2 cut(s) | 86, 214 |
| BbsI | GAAGAC | 1 cut(s) | 241 |
| BbvI | GCAGC | 1 cut(s) | 43 |
| BclI | TGATCA | 1 cut(s) | 204 |
| BcoDI | GTCTC | 1 cut(s) | 349 |
| BfmI | CTRYAG | 1 cut(s) | 57 |
| BglII | AGATCT | 1 cut(s) | 357 |
| BisI | GCNGC | 1 cut(s) | 57 |
| BlsI | GCNGC | 1 cut(s) | 58 |
| BpiI | GAAGAC | 1 cut(s) | 241 |
| BpuEI | CTTGAG | 1 cut(s) | 111 |
| BsaI | GGTCTC | 1 cut(s) | 349 |
| BseGI | GGATG | 1 cut(s) | 271 |
| BseRI | GAGGAG | 1 cut(s) | 26 |
| BseXI | GCAGC | 1 cut(s) | 43 |
| BsmAI | GTCTC | 1 cut(s) | 349 |
| Bso31I | GGTCTC | 1 cut(s) | 349 |
| Bsp143I | GATC | 5 cut(s) | 138, 189, 204, 323, 357 |
| BspACI | CCGC | 1 cut(s) | 152 |
| BspMAI | CTGCAG | 1 cut(s) | 61 |
| BspPI | GGATC | 1 cut(s) | 331 |
| BspTNI | GGTCTC | 1 cut(s) | 349 |
| BssMI | GATC | 5 cut(s) | 138, 189, 204, 323, 357 |
| BssNAI | GTATAC | 1 cut(s) | 163 |
| Bst1107I | GTATAC | 1 cut(s) | 163 |
| Bst6I | CTCTTC | 1 cut(s) | 51 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 29, 149 |
| BstF5I | GGATG | 1 cut(s) | 271 |
| BstKTI | GATC | 5 cut(s) | 141, 192, 207, 326, 360 |
| BstMAI | GTCTC | 1 cut(s) | 349 |
| BstMBI | GATC | 5 cut(s) | 138, 189, 204, 323, 357 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 29, 149 |
| BstSFI | CTRYAG | 1 cut(s) | 57 |
| BstV1I | GCAGC | 1 cut(s) | 43 |
| BstV2I | GAAGAC | 1 cut(s) | 241 |
| BstX2I | RGATCY | 2 cut(s) | 323, 357 |
| BstYI | RGATCY | 2 cut(s) | 323, 357 |
| BstZ17I | GTATAC | 1 cut(s) | 163 |
| BtsCI | GGATG | 1 cut(s) | 271 |
| CviJI | RGCY | 3 cut(s) | 56, 171, 305 |
| CviKI_1 | RGCY | 3 cut(s) | 56, 171, 305 |
| DpnI | GATC | 5 cut(s) | 140, 191, 206, 325, 359 |
| DpnII | GATC | 5 cut(s) | 138, 189, 204, 323, 357 |
| DraI | TTTAAA | 1 cut(s) | 334 |
| DraIII | CACNNNGTG | 1 cut(s) | 92 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 128, 257 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 128, 257 |
| Eam1104I | CTCTTC | 1 cut(s) | 51 |
| EarI | CTCTTC | 1 cut(s) | 51 |
| Eco31I | GGTCTC | 1 cut(s) | 349 |
| FaiI | YATR | 4 cut(s) | 150, 163, 180, 243 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 310, 342 |
| FbaI | TGATCA | 1 cut(s) | 204 |
| FblI | GTMKAC | 2 cut(s) | 63, 162 |
| Fnu4HI | GCNGC | 1 cut(s) | 57 |
| FokI | GGATG | 1 cut(s) | 278 |
| Fsp4HI | GCNGC | 1 cut(s) | 57 |
| GluI | GCNGC | 1 cut(s) | 57 |
| HinfI | GANTC | 4 cut(s) | 4, 77, 227, 258 |
| HphI | GGTGA | 2 cut(s) | 86, 214 |
| Hpy166II | GTNNAC | 2 cut(s) | 64, 163 |
| Hpy188I | TCNGA | 2 cut(s) | 198, 357 |
| Hpy188III | TCNNGA | 2 cut(s) | 231, 313 |
| Hpy8I | GTNNAC | 2 cut(s) | 64, 163 |
| HpyAV | CCTTC | 1 cut(s) | 193 |
| HpyCH4IV | ACGT | 1 cut(s) | 252 |
| HpyCH4V | TGCA | 2 cut(s) | 23, 59 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 29, 149 |
| HpySE526I | ACGT | 1 cut(s) | 252 |
| Ksp22I | TGATCA | 1 cut(s) | 204 |
| Kzo9I | GATC | 5 cut(s) | 138, 189, 204, 323, 357 |
| LmnI | GCTCC | 1 cut(s) | 88 |
| LpnPI | CCDG | 1 cut(s) | 216 |
| Lsp1109I | GCAGC | 1 cut(s) | 43 |
| MaeII | ACGT | 1 cut(s) | 252 |
| MaeIII | GTNAC | 2 cut(s) | 74, 130 |
| MalI | GATC | 5 cut(s) | 140, 191, 206, 325, 359 |
| MboI | GATC | 5 cut(s) | 138, 189, 204, 323, 357 |
| MboII | GAAGA | 3 cut(s) | 38, 121, 246 |
| MflI | RGATCY | 2 cut(s) | 323, 357 |
| MluCI | AATT | 3 cut(s) | 222, 338, 364 |
| MlyI | GAGTC | 3 cut(s) | 13, 71, 267 |
| MnlI | CCTC | 6 cut(s) | 18, 26, 47, 54, 135, 314 |
| MseI | TTAA | 3 cut(s) | 156, 333, 367 |
| MspA1I | CMGCKG | 1 cut(s) | 56 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 29, 149 |
| NdeII | GATC | 5 cut(s) | 138, 189, 204, 323, 357 |
| NmuCI | GTSAC | 2 cut(s) | 74, 130 |
| PfeI | GAWTC | 1 cut(s) | 227 |
| PkrI | GCNGC | 1 cut(s) | 58 |
| PleI | GAGTC | 3 cut(s) | 12, 71, 266 |
| PpsI | GAGTC | 3 cut(s) | 12, 71, 266 |
| PsiI | TTATAA | 1 cut(s) | 243 |
| PstI | CTGCAG | 1 cut(s) | 61 |
| PsuI | RGATCY | 2 cut(s) | 323, 357 |
| PvuII | CAGCTG | 1 cut(s) | 56 |
| SaqAI | TTAA | 3 cut(s) | 156, 333, 367 |
| SatI | GCNGC | 1 cut(s) | 57 |
| Sau3AI | GATC | 5 cut(s) | 138, 189, 204, 323, 357 |
| SchI | GAGTC | 3 cut(s) | 13, 71, 267 |
| SetI | ASST | 5 cut(s) | 18, 58, 204, 255, 307 |
| SfcI | CTRYAG | 1 cut(s) | 57 |
| SmlI | CTYRAG | 1 cut(s) | 126 |
| SmoI | CTYRAG | 1 cut(s) | 126 |
| Sse9I | AATT | 3 cut(s) | 222, 338, 364 |
| SsiI | CCGC | 1 cut(s) | 152 |
| TaiI | ACGT | 1 cut(s) | 255 |
| TasI | AATT | 3 cut(s) | 222, 338, 364 |
| TfiI | GAWTC | 1 cut(s) | 227 |
| Tru1I | TTAA | 3 cut(s) | 156, 333, 367 |
| Tru9I | TTAA | 3 cut(s) | 156, 333, 367 |
| TseFI | GTSAC | 2 cut(s) | 74, 130 |
| TseI | GCWGC | 1 cut(s) | 56 |
| Tsp45I | GTSAC | 2 cut(s) | 74, 130 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 93 |
| XmiI | GTMKAC | 2 cut(s) | 63, 162 |