Rmu_sc0003573.1_g000025

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003573.1
OG0025565
Physical Location & Seq
Reverse (-)
108690 .. 109289
600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003573.1_g000025.1.cds

Sequence Viewer

Length: 552 bp
atgaccgacgccattgtaagcctcatggttgcgttaaacctatctgactttgctagggtttccctggttctgcggggagggcagcgttgcaggacaaccattggtacatggttggttgattgcttggcttttcatggaggaccttggctttacaggaatactatgctggtgttaggggagtatgatggagttggtccagtggaggctattccactgagaatgatggagacttgggtggcggtaaaaggacttccattgggagtgatttattttgatcaaccggcactccgccacaagaaagaggagcaaagcatccgtctgatctttgacactcgtcgaaggatacgaacttggatcctattagagatctctcctttggccgaaccaaaaatcaacttgatctatgggaagattaaaaggttttgcagggattgtggggtatttgaacacgacgtctccggttatgacaaccttcttgtgaaggagaaggagcaattgcaaggaagaccaccgacgttggcgttggtagggttgtcgatgtccgctaggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

183

Amino Acids

20.74

Weight (kDa)

9.16

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025565)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003573.1_g000025
rosa_rugosa Rorug04G0081200 Rorug07G0298200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 456
AciI CCGC 4 cut(s) 73, 239, 289, 543
AclWI GGATC 2 cut(s) 349, 362
AcoI YGGCCR 1 cut(s) 378
AcyI GRCGYC 2 cut(s) 9, 453
AfaI GTAC 1 cut(s) 106
AgsI TTSAA 1 cut(s) 446
AjnI CCWGG 1 cut(s) 63
Alw26I GTCTC 2 cut(s) 221, 460
AlwI GGATC 2 cut(s) 349, 362
AoxI GGCC 1 cut(s) 378
ApeKI GCWGC 1 cut(s) 82
ArsI GACNNNNNNTTYG 2 cut(s) 301, 333
AspS9I GGNCC 2 cut(s) 140, 194
AvaII GGWCC 2 cut(s) 140, 194
BamHI GGATCC 1 cut(s) 354
BarI GAAGNNNNNNTAC 2 cut(s) 234, 266
BbsI GAAGAC 1 cut(s) 511
BbvI GCAGC 1 cut(s) 94
BccI CCATC 2 cut(s) 179, 217
BciT130I CCWGG 1 cut(s) 65
BciVI GTATCC 1 cut(s) 336
BclI TGATCA 1 cut(s) 274
BcoDI GTCTC 2 cut(s) 221, 460
BfaI CTAG 2 cut(s) 54, 546
BfuI GTATCC 1 cut(s) 336
BglII AGATCT 1 cut(s) 366
BisI GCNGC 1 cut(s) 83
BlsI GCNGC 1 cut(s) 84
Bme1390I CCNGG 1 cut(s) 65
Bme18I GGWCC 2 cut(s) 140, 194
BmgT120I GGNCC 2 cut(s) 140, 194
BmiI GGNNCC 1 cut(s) 356
BmrFI CCNGG 1 cut(s) 65
BmsI GCATC 1 cut(s) 321
BoxI GACNNNNGTC 1 cut(s) 333
BpiI GAAGAC 1 cut(s) 511
BsaHI GRCGYC 2 cut(s) 9, 453
BsaJI CCNNGG 2 cut(s) 63, 143
BsaWI WCCGGW 1 cut(s) 458
BsaXI ACNNNNNCTCC 4 cut(s) 270, 300, 440, 470
Bse118I RCCGGY 1 cut(s) 280
Bse1I ACTGG 1 cut(s) 197
BseBI CCWGG 1 cut(s) 65
BseDI CCNNGG 2 cut(s) 63, 143
BseGI GGATG 1 cut(s) 312
BseMII CTCAG 1 cut(s) 206
BseNI ACTGG 1 cut(s) 197
BseRI GAGGAG 1 cut(s) 317
BseXI GCAGC 1 cut(s) 94
BshFI GGCC 1 cut(s) 380
BsiSI CCGG 2 cut(s) 281, 459
BsmAI GTCTC 2 cut(s) 221, 460
BsmBI CGTCTC 1 cut(s) 460
BsnI GGCC 1 cut(s) 380
Bsp143I GATC 5 cut(s) 274, 321, 354, 366, 399
BspACI CCGC 4 cut(s) 73, 239, 289, 543
BspANI GGCC 1 cut(s) 380
BspCNI CTCAG 1 cut(s) 207
BspLI GGNNCC 1 cut(s) 356
BspPI GGATC 2 cut(s) 349, 362
BsrFI RCCGGY 1 cut(s) 280
BsrI ACTGG 1 cut(s) 197
BssAI RCCGGY 1 cut(s) 280
BssECI CCNNGG 2 cut(s) 63, 143
BssMI GATC 5 cut(s) 274, 321, 354, 366, 399
BssNI GRCGYC 2 cut(s) 9, 453
BssT1I CCWWGG 1 cut(s) 143
Bst2UI CCWGG 1 cut(s) 65
BstACI GRCGYC 2 cut(s) 9, 453
BstDEI CTNAG 1 cut(s) 215
BstF5I GGATG 1 cut(s) 312
BstKTI GATC 5 cut(s) 277, 324, 357, 369, 402
BstMAI GTCTC 2 cut(s) 221, 460
BstMBI GATC 5 cut(s) 274, 321, 354, 366, 399
BstMWI GCNNNNNNNGC 1 cut(s) 79
BstNI CCWGG 1 cut(s) 65
BstPAI GACNNNNGTC 1 cut(s) 333
BstSCI CCNGG 1 cut(s) 63
BstV1I GCAGC 1 cut(s) 94
BstV2I GAAGAC 1 cut(s) 511
BstX2I RGATCY 2 cut(s) 354, 366
BstYI RGATCY 2 cut(s) 354, 366
BsuI GTATCC 1 cut(s) 336
BsuRI GGCC 1 cut(s) 380
BtsCI GGATG 1 cut(s) 312
BtsIMutI CAGTG 2 cut(s) 204, 212
Cfr10I RCCGGY 1 cut(s) 280
Cfr13I GGNCC 2 cut(s) 140, 194
CseI GACGC 1 cut(s) 17
Csp6I GTAC 1 cut(s) 105
CspCI CAANNNNNGTGG 2 cut(s) 498, 533
CviAII CATG 3 cut(s) 25, 108, 134
CviJI RGCY 5 cut(s) 21, 128, 148, 206, 380
CviKI_1 RGCY 5 cut(s) 21, 128, 148, 206, 380
CviQI GTAC 1 cut(s) 105
DdeI CTNAG 1 cut(s) 215
DpnI GATC 5 cut(s) 276, 323, 356, 368, 401
DpnII GATC 5 cut(s) 274, 321, 354, 366, 399
EaeI YGGCCR 1 cut(s) 378
EciI GGCGGA 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 143
Eco47I GGWCC 2 cut(s) 140, 194
EcoO109I RGGNCCY 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 63
EcoT14I CCWWGG 1 cut(s) 143
ErhI CCWWGG 1 cut(s) 143
Esp3I CGTCTC 1 cut(s) 460
FaeI CATG 3 cut(s) 28, 111, 137
FaiI YATR 7 cut(s) 26, 109, 135, 164, 183, 405, 465
FatI CATG 3 cut(s) 24, 107, 133
FauI CCCGC 1 cut(s) 66
FbaI TGATCA 1 cut(s) 274
Fnu4HI GCNGC 1 cut(s) 83
FokI GGATG 1 cut(s) 299
Fsp4HI GCNGC 1 cut(s) 83
FspBI CTAG 2 cut(s) 54, 546
GluI GCNGC 1 cut(s) 83
HaeIII GGCC 1 cut(s) 380
HapII CCGG 2 cut(s) 281, 459
HgaI GACGC 1 cut(s) 17
Hin1I GRCGYC 2 cut(s) 9, 453
Hin1II CATG 3 cut(s) 28, 111, 137
HpaII CCGG 2 cut(s) 281, 459
Hpy188I TCNGA 2 cut(s) 46, 321
Hpy99I CGWCG 4 cut(s) 11, 339, 455, 517
HpyAV CCTTC 4 cut(s) 333, 475, 481, 482
HpyCH4IV ACGT 2 cut(s) 453, 515
HpyCH4V TGCA 3 cut(s) 90, 426, 499
HpyF10VI GCNNNNNNNGC 1 cut(s) 79
HpyF3I CTNAG 1 cut(s) 215
HpySE526I ACGT 2 cut(s) 453, 515
Hsp92I GRCGYC 2 cut(s) 9, 453
Hsp92II CATG 3 cut(s) 28, 111, 137
Ksp22I TGATCA 1 cut(s) 274
Kzo9I GATC 5 cut(s) 274, 321, 354, 366, 399
LmnI GCTCC 2 cut(s) 304, 490
LpnPI CCDG 9 cut(s) 50, 76, 77, 139, 152, 210, 294, 412, 472
Lsp1109I GCAGC 1 cut(s) 94
LweI GCATC 1 cut(s) 321
MaeI CTAG 2 cut(s) 54, 546
MaeII ACGT 2 cut(s) 453, 515
MalI GATC 5 cut(s) 276, 323, 356, 368, 401
MboI GATC 5 cut(s) 274, 321, 354, 366, 399
MboII GAAGA 2 cut(s) 421, 516
MfeI CAATTG 1 cut(s) 494
MflI RGATCY 2 cut(s) 354, 366
MluCI AATT 1 cut(s) 494
MnlI CCTC 5 cut(s) 32, 71, 131, 196, 295
MseI TTAA 2 cut(s) 35, 414
MspI CCGG 2 cut(s) 281, 459
MspR9I CCNGG 1 cut(s) 65
MunI CAATTG 1 cut(s) 494
MvaI CCWGG 1 cut(s) 65
MwoI GCNNNNNNNGC 1 cut(s) 79
NdeII GATC 5 cut(s) 274, 321, 354, 366, 399
NlaIII CATG 3 cut(s) 28, 111, 137
NlaIV GGNNCC 1 cut(s) 356
PcsI WCGNNNNNNNCGW 1 cut(s) 343
PkrI GCNGC 1 cut(s) 84
PpuMI RGGWCCY 1 cut(s) 140
PshAI GACNNNNGTC 1 cut(s) 333
Psp5II RGGWCCY 1 cut(s) 140
Psp6I CCWGG 1 cut(s) 63
PspGI CCWGG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 356
PspPI GGNCC 2 cut(s) 140, 194
PspPPI RGGWCCY 1 cut(s) 140
PsuI RGATCY 2 cut(s) 354, 366
RsaI GTAC 1 cut(s) 106
RsaNI GTAC 1 cut(s) 105
SaqAI TTAA 2 cut(s) 35, 414
SatI GCNGC 1 cut(s) 83
Sau3AI GATC 5 cut(s) 274, 321, 354, 366, 399
Sau96I GGNCC 2 cut(s) 140, 194
ScrFI CCNGG 1 cut(s) 65
SetI ASST 7 cut(s) 42, 145, 422, 456, 474, 518, 551
SfaNI GCATC 1 cut(s) 321
SinI GGWCC 2 cut(s) 140, 194
Sse9I AATT 1 cut(s) 494
SsiI CCGC 4 cut(s) 73, 239, 289, 543
SspMI CTAG 2 cut(s) 54, 546
StyD4I CCNGG 1 cut(s) 63
StyI CCWWGG 1 cut(s) 143
TaiI ACGT 2 cut(s) 456, 518
TaqI TCGA 2 cut(s) 337, 536
TaqII GACCGA 1 cut(s) 20
TasI AATT 1 cut(s) 494
Tru1I TTAA 2 cut(s) 35, 414
Tru9I TTAA 2 cut(s) 35, 414
TscAI CASTG 2 cut(s) 204, 219
TseI GCWGC 1 cut(s) 82
TspDTI ATGAA 1 cut(s) 122
TspGWI ACGGA 1 cut(s) 305
TspRI CASTG 2 cut(s) 204, 219
VpaK11BI GGWCC 2 cut(s) 140, 194
XspI CTAG 2 cut(s) 54, 546
ZraI GACGTC 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.