Rmu_sc0003596.1_g000001

Nematode resistance protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003596.1
OG0031271
Physical Location & Seq
Forward (+)
2931 .. 5618
2688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003596.1_g000001.1.cds

Sequence Viewer

Length: 579 bp
atgagaagctccggcgaaaaatctaagaagaggctgatgaaggtggttcacatatcaaacccgttgaatgttacaacgagcgcctctgaatttaggactttgcgctgggttgcttcctttgtgggggtaactctgaagcccagtaccttattcgccgcacttagtggcgtcagggtcctcgagcccaagcgcgtgaactgcggcatcgagtatagcgacttcgaaaaggtcgcagctgactgttacatctggaagcttctcatggaaatcgaagacctccacctattaatggacccaaaggactttctccggctcaagaaccagttgaagatcaaaacggtggacgatacagagtcgttttgctttagatcgaagggactggtggagatcaccaagctgtgtaaggacttgaagcataagacgcaggaatccggtcaccagtccggtagcccgattccgatgaccgatagccggatttcggtcattggtcgcctgattccggtcattggtcaccggagtccggtcaccggtcgccggtcgccggagtccgccaccggagtcaagcaaggtctccaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hs1pro-1_C Hs1pro-1_N

Protein Analysis

192

Amino Acids

21.5

Weight (kDa)

9.69

Isoelectric Point (pI)

53.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031271)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003596.1_g000001 Rmu_sc0008921.1_g000015

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 192
AciI CCGC 3 cut(s) 156, 201, 549
AcsI RAATTY 1 cut(s) 89
AcuI CTGAAG 1 cut(s) 155
AcyI GRCGYC 1 cut(s) 168
AdeI CACNNNGTG 1 cut(s) 164
AfaI GTAC 1 cut(s) 145
AgeI ACCGGT 1 cut(s) 527
AgsI TTSAA 3 cut(s) 67, 328, 412
AluBI AGCT 4 cut(s) 9, 236, 256, 397
AluI AGCT 4 cut(s) 9, 236, 256, 397
Alw26I GTCTC 1 cut(s) 575
Ama87I CYCGRG 1 cut(s) 179
ApeKI GCWGC 1 cut(s) 233
ApoI RAATTY 1 cut(s) 89
AseI ATTAAT 1 cut(s) 287
AsiGI ACCGGT 1 cut(s) 527
AspLEI GCGC 3 cut(s) 83, 105, 192
AspS9I GGNCC 2 cut(s) 175, 292
AsuHPI GGTGA 4 cut(s) 382, 428, 503, 517
AsuII TTCGAA 1 cut(s) 222
AvaI CYCGRG 1 cut(s) 179
AvaII GGWCC 2 cut(s) 175, 292
BanII GRGCYC 1 cut(s) 186
BarI GAAGNNNNNNTAC 2 cut(s) 203, 235
BbsI GAAGAC 1 cut(s) 279
BbvI GCAGC 1 cut(s) 245
BcoDI GTCTC 1 cut(s) 575
BfoI RGCGCY 1 cut(s) 84
BisI GCNGC 3 cut(s) 156, 202, 234
BlsI GCNGC 3 cut(s) 157, 203, 235
Bme18I GGWCC 2 cut(s) 175, 292
BmeT110I CYCGRG 1 cut(s) 179
BmgT120I GGNCC 2 cut(s) 175, 292
BmiI GGNNCC 2 cut(s) 176, 294
BmrI ACTGGG 1 cut(s) 135
BmsI GCATC 1 cut(s) 213
BmuI ACTGGG 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 279
Bpu14I TTCGAA 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 299
BsaHI GRCGYC 1 cut(s) 168
BsaI GGTCTC 1 cut(s) 575
BsaWI WCCGGW 7 cut(s) 431, 443, 499, 513, 520, 527, 554
Bse118I RCCGGY 2 cut(s) 527, 534
Bse1I ACTGG 4 cut(s) 141, 322, 384, 439
BseNI ACTGG 4 cut(s) 141, 322, 384, 439
BseXI GCAGC 1 cut(s) 245
BseYI CCCAGC 1 cut(s) 105
Bsh1236I CGCG 1 cut(s) 192
Bsh1285I CGRYCG 2 cut(s) 532, 539
BshTI ACCGGT 1 cut(s) 527
BsiEI CGRYCG 2 cut(s) 532, 539
BsiHKCI CYCGRG 1 cut(s) 179
BslFI GGGAC 1 cut(s) 390
BsmAI GTCTC 1 cut(s) 575
BsmFI GGGAC 1 cut(s) 390
Bso31I GGTCTC 1 cut(s) 575
BsoBI CYCGRG 1 cut(s) 179
Bsp119I TTCGAA 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 3 cut(s) 330, 368, 387
BspACI CCGC 3 cut(s) 156, 201, 549
BspFNI CGCG 1 cut(s) 192
BspLI GGNNCC 2 cut(s) 176, 294
BspT104I TTCGAA 1 cut(s) 222
BspTNI GGTCTC 1 cut(s) 575
BsrFI RCCGGY 2 cut(s) 527, 534
BsrI ACTGG 4 cut(s) 141, 322, 384, 439
BssAI RCCGGY 2 cut(s) 527, 534
BssMI GATC 3 cut(s) 330, 368, 387
BssNI GRCGYC 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 242, 340
Bst6I CTCTTC 1 cut(s) 23
BstACI GRCGYC 1 cut(s) 168
BstBI TTCGAA 1 cut(s) 222
BstDEI CTNAG 2 cut(s) 24, 161
BstEII GGTNACC 3 cut(s) 434, 509, 523
BstFNI CGCG 1 cut(s) 192
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 3 cut(s) 83, 105, 192
BstKTI GATC 3 cut(s) 333, 371, 390
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 3 cut(s) 330, 368, 387
BstMCI CGRYCG 2 cut(s) 532, 539
BstMWI GCNNNNNNNGC 2 cut(s) 198, 421
BstPI GGTNACC 3 cut(s) 434, 509, 523
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 1 cut(s) 245
BstV2I GAAGAC 1 cut(s) 279
CfoI GCGC 3 cut(s) 83, 105, 192
Cfr10I RCCGGY 2 cut(s) 527, 534
Cfr13I GGNCC 2 cut(s) 175, 292
CseI GACGC 2 cut(s) 157, 430
Csp6I GTAC 1 cut(s) 144
CspAI ACCGGT 1 cut(s) 527
CviAII CATG 1 cut(s) 262
CviQI GTAC 1 cut(s) 144
DdeI CTNAG 2 cut(s) 24, 161
DpnI GATC 3 cut(s) 332, 370, 389
DpnII GATC 3 cut(s) 330, 368, 387
DraIII CACNNNGTG 1 cut(s) 164
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
EciI GGCGGA 1 cut(s) 538
Eco24I GRGCYC 1 cut(s) 186
Eco31I GGTCTC 1 cut(s) 575
Eco47I GGWCC 2 cut(s) 175, 292
Eco57I CTGAAG 1 cut(s) 155
Eco88I CYCGRG 1 cut(s) 179
Eco91I GGTNACC 3 cut(s) 434, 509, 523
EcoO109I RGGNCCY 1 cut(s) 175
EcoO65I GGTNACC 3 cut(s) 434, 509, 523
EcoT38I GRGCYC 1 cut(s) 186
FaeI CATG 1 cut(s) 265
FaiI YATR 4 cut(s) 53, 213, 263, 417
FaqI GGGAC 1 cut(s) 390
FatI CATG 1 cut(s) 261
Fnu4HI GCNGC 3 cut(s) 156, 202, 234
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 3 cut(s) 156, 202, 234
GlaI GCGC 3 cut(s) 82, 104, 191
GluI GCNGC 3 cut(s) 156, 202, 234
GsaI CCCAGC 1 cut(s) 109
HaeII RGCGCY 1 cut(s) 84
HgaI GACGC 2 cut(s) 157, 430
HhaI GCGC 3 cut(s) 83, 105, 192
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 1 cut(s) 265
Hin6I GCGC 3 cut(s) 81, 103, 190
HinP1I GCGC 3 cut(s) 81, 103, 190
HindIII AAGCTT 1 cut(s) 254
HinfI GANTC 7 cut(s) 353, 428, 454, 496, 517, 545, 558
HphI GGTGA 4 cut(s) 382, 428, 503, 517
Hpy166II GTNNAC 3 cut(s) 49, 196, 343
Hpy188I TCNGA 3 cut(s) 88, 135, 459
Hpy188III TCNNGA 2 cut(s) 250, 316
Hpy8I GTNNAC 3 cut(s) 49, 196, 343
HpyAV CCTTC 2 cut(s) 34, 367
HpyCH4III ACNGT 2 cut(s) 242, 340
HpyF10VI GCNNNNNNNGC 2 cut(s) 198, 421
HpyF3I CTNAG 2 cut(s) 24, 161
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 1 cut(s) 265
HspAI GCGC 3 cut(s) 81, 103, 190
Kzo9I GATC 3 cut(s) 330, 368, 387
LmnI GCTCC 1 cut(s) 14
Lsp1109I GCAGC 1 cut(s) 245
LweI GCATC 1 cut(s) 213
MaeIII GTNAC 6 cut(s) 70, 127, 242, 434, 509, 523
MalI GATC 3 cut(s) 332, 370, 389
MboI GATC 3 cut(s) 330, 368, 387
MboII GAAGA 3 cut(s) 40, 284, 340
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 1 cut(s) 89
MlyI GAGTC 4 cut(s) 362, 526, 554, 567
MnlI CCTC 4 cut(s) 24, 94, 188, 287
MseI TTAA 1 cut(s) 287
MspA1I CMGCKG 1 cut(s) 236
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 2 cut(s) 198, 421
NdeII GATC 3 cut(s) 330, 368, 387
NlaIII CATG 1 cut(s) 265
NlaIV GGNNCC 2 cut(s) 176, 294
NmuCI GTSAC 3 cut(s) 434, 509, 523
NspV TTCGAA 1 cut(s) 222
PaeR7I CTCGAG 1 cut(s) 179
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PfeI GAWTC 3 cut(s) 428, 454, 496
PinAI ACCGGT 1 cut(s) 527
PkrI GCNGC 3 cut(s) 157, 203, 235
PleI GAGTC 4 cut(s) 361, 525, 553, 566
PpsI GAGTC 4 cut(s) 361, 525, 553, 566
PpuMI RGGWCCY 1 cut(s) 175
PshBI ATTAAT 1 cut(s) 287
Psp5II RGGWCCY 1 cut(s) 175
PspEI GGTNACC 3 cut(s) 434, 509, 523
PspFI CCCAGC 1 cut(s) 105
PspN4I GGNNCC 2 cut(s) 176, 294
PspPI GGNCC 2 cut(s) 175, 292
PspPPI RGGWCCY 1 cut(s) 175
PspXI VCTCGAGB 1 cut(s) 179
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 1 cut(s) 145
RsaNI GTAC 1 cut(s) 144
SaqAI TTAA 1 cut(s) 287
SatI GCNGC 3 cut(s) 156, 202, 234
Sau3AI GATC 3 cut(s) 330, 368, 387
Sau96I GGNCC 2 cut(s) 175, 292
SchI GAGTC 4 cut(s) 362, 526, 554, 567
SduI GDGCHC 1 cut(s) 186
SfaNI GCATC 1 cut(s) 213
Sfr274I CTCGAG 1 cut(s) 179
SfuI TTCGAA 1 cut(s) 222
SinI GGWCC 2 cut(s) 175, 292
SlaI CTCGAG 1 cut(s) 179
SmlI CTYRAG 2 cut(s) 179, 314
SmoI CTYRAG 2 cut(s) 179, 314
Sse9I AATT 1 cut(s) 89
SsiI CCGC 3 cut(s) 156, 201, 549
TaaI ACNGT 2 cut(s) 242, 340
TaqI TCGA 5 cut(s) 180, 207, 222, 270, 371
TaqII GACCGA 2 cut(s) 469, 479
TasI AATT 1 cut(s) 89
TauI GCSGC 2 cut(s) 158, 204
TfiI GAWTC 3 cut(s) 428, 454, 496
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TseFI GTSAC 3 cut(s) 434, 509, 523
TseI GCWGC 1 cut(s) 233
Tsp45I GTSAC 3 cut(s) 434, 509, 523
TspDTI ATGAA 1 cut(s) 53
VpaK11BI GGWCC 2 cut(s) 175, 292
VspI ATTAAT 1 cut(s) 287
XapI RAATTY 1 cut(s) 89
XhoI CTCGAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.