Rmu_sc0008921.1_g000015

Nematode resistance protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008921.1
OG0031271
Physical Location & Seq
Reverse (-)
36744 .. 40464
3721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008921.1_g000015.1.cds

Sequence Viewer

Length: 714 bp
atgagaagctccggcgacaaatctaagaagaggctgatgaaggtggttcacatatcaaacccgttgaatgttacaacgagcgcctctgaatttaggactttggttcagcaactcaccagccacgataccgacgccctactagatctgaacgacttcatgaaattttccaattatgctttcttcctccacgcttcgaatcgttgcaagtggtttggagagcatgaagtgtggttcaaggcgcttccaaagaacctagtttggtcgcgtgtcctcgagcccaagcgcgtgaactgcggcatcgagtatagcgacttcgaaaaggtcgcagctgactgttacatctggaagcttctcatggaaatcgaagacctccacctattaatggacccaaaggactttctccggctcaagaaccagttgaagatcaaaacggtggacgatacagagtcgttttgctttagatcgaagggactggtggagatcaccaagctgtgtaaggacttgaagcataagacgcaggaatccggtcaccaatccggtagccggattccgatgaccgatagccggatttcgatcattggtcgcctgattcctgtcattggtcaccggagtccagtcaccggtcaccggtcgccggcgtcgggtcaccggtcgccggcgtcgggtcaccggtcgccagagtccgccaccggagtcaagcaaggtctccaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hs1pro-1_C Hs1pro-1_N

Protein Analysis

237

Amino Acids

26.96

Weight (kDa)

9.45

Isoelectric Point (pI)

49.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031271)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003596.1_g000001 Rmu_sc0008921.1_g000015

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 265, 285
AciI CCGC 2 cut(s) 294, 684
AcsI RAATTY 2 cut(s) 89, 161
AcyI GRCGYC 3 cut(s) 132, 638, 659
AgeI ACCGGT 4 cut(s) 620, 627, 648, 669
AgsI TTSAA 4 cut(s) 67, 235, 421, 505
AjuI GAANNNNNNNTTGG 2 cut(s) 161, 193
AluBI AGCT 4 cut(s) 9, 329, 349, 490
AluI AGCT 4 cut(s) 9, 329, 349, 490
Alw26I GTCTC 1 cut(s) 710
Ama87I CYCGRG 1 cut(s) 272
ApeKI GCWGC 1 cut(s) 326
ApoI RAATTY 2 cut(s) 89, 161
AseI ATTAAT 1 cut(s) 380
AsiGI ACCGGT 4 cut(s) 620, 627, 648, 669
Asp700I GAANNNNTTC 1 cut(s) 152
AspLEI GCGC 3 cut(s) 83, 241, 285
AspS9I GGNCC 1 cut(s) 385
AsuHPI GGTGA 8 cut(s) 106, 475, 521, 596, 610, 617, 638, 659
AsuII TTCGAA 2 cut(s) 194, 315
AvaI CYCGRG 1 cut(s) 272
AvaII GGWCC 1 cut(s) 385
BanII GRGCYC 1 cut(s) 279
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
BbsI GAAGAC 1 cut(s) 372
BbvI GCAGC 1 cut(s) 338
BcoDI GTCTC 1 cut(s) 710
BfaI CTAG 2 cut(s) 140, 254
BfoI RGCGCY 2 cut(s) 84, 242
BglII AGATCT 1 cut(s) 142
BisI GCNGC 2 cut(s) 295, 327
BlsI GCNGC 2 cut(s) 296, 328
Bme18I GGWCC 1 cut(s) 385
BmeT110I CYCGRG 1 cut(s) 272
BmgT120I GGNCC 1 cut(s) 385
BmiI GGNNCC 1 cut(s) 387
BmsI GCATC 1 cut(s) 306
BpiI GAAGAC 1 cut(s) 372
Bpu14I TTCGAA 2 cut(s) 194, 315
BpuEI CTTGAG 1 cut(s) 392
BsaBI GATNNNNATC 1 cut(s) 572
BsaHI GRCGYC 3 cut(s) 132, 638, 659
BsaI GGTCTC 1 cut(s) 710
BsaWI WCCGGW 8 cut(s) 524, 536, 606, 620, 627, 648, 669, 689
Bse118I RCCGGY 6 cut(s) 620, 627, 634, 648, 655, 669
Bse1I ACTGG 3 cut(s) 415, 477, 614
Bse8I GATNNNNATC 1 cut(s) 572
BseJI GATNNNNATC 1 cut(s) 572
BseNI ACTGG 3 cut(s) 415, 477, 614
BseXI GCAGC 1 cut(s) 338
Bsh1236I CGCG 2 cut(s) 265, 285
Bsh1285I CGRYCG 3 cut(s) 632, 653, 674
BshTI ACCGGT 4 cut(s) 620, 627, 648, 669
BsiEI CGRYCG 3 cut(s) 632, 653, 674
BsiHKCI CYCGRG 1 cut(s) 272
BslFI GGGAC 1 cut(s) 483
BsmAI GTCTC 1 cut(s) 710
BsmFI GGGAC 1 cut(s) 483
Bso31I GGTCTC 1 cut(s) 710
BsoBI CYCGRG 1 cut(s) 272
Bsp119I TTCGAA 2 cut(s) 194, 315
Bsp1286I GDGCHC 1 cut(s) 279
Bsp143I GATC 5 cut(s) 142, 423, 461, 480, 573
BspACI CCGC 2 cut(s) 294, 684
BspFNI CGCG 2 cut(s) 265, 285
BspHI TCATGA 1 cut(s) 156
BspLI GGNNCC 1 cut(s) 387
BspT104I TTCGAA 2 cut(s) 194, 315
BspTNI GGTCTC 1 cut(s) 710
BsrFI RCCGGY 6 cut(s) 620, 627, 634, 648, 655, 669
BsrI ACTGG 3 cut(s) 415, 477, 614
BssAI RCCGGY 6 cut(s) 620, 627, 634, 648, 655, 669
BssMI GATC 5 cut(s) 142, 423, 461, 480, 573
BssNI GRCGYC 3 cut(s) 132, 638, 659
Bst4CI ACNGT 2 cut(s) 335, 433
Bst6I CTCTTC 1 cut(s) 23
BstACI GRCGYC 3 cut(s) 132, 638, 659
BstBI TTCGAA 2 cut(s) 194, 315
BstC8I GCNNGC 2 cut(s) 636, 657
BstDEI CTNAG 1 cut(s) 24
BstEII GGTNACC 5 cut(s) 527, 602, 623, 644, 665
BstFNI CGCG 2 cut(s) 265, 285
BstH2I RGCGCY 2 cut(s) 84, 242
BstHHI GCGC 3 cut(s) 83, 241, 285
BstKTI GATC 5 cut(s) 145, 426, 464, 483, 576
BstMAI GTCTC 1 cut(s) 710
BstMBI GATC 5 cut(s) 142, 423, 461, 480, 573
BstMCI CGRYCG 3 cut(s) 632, 653, 674
BstMWI GCNNNNNNNGC 2 cut(s) 291, 514
BstPI GGTNACC 5 cut(s) 527, 602, 623, 644, 665
BstUI CGCG 2 cut(s) 265, 285
BstV1I GCAGC 1 cut(s) 338
BstV2I GAAGAC 1 cut(s) 372
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
Cac8I GCNNGC 2 cut(s) 636, 657
CciI TCATGA 1 cut(s) 156
CfoI GCGC 3 cut(s) 83, 241, 285
Cfr10I RCCGGY 6 cut(s) 620, 627, 634, 648, 655, 669
Cfr13I GGNCC 1 cut(s) 385
CseI GACGC 4 cut(s) 140, 523, 627, 648
CspAI ACCGGT 4 cut(s) 620, 627, 648, 669
CviAII CATG 3 cut(s) 157, 221, 355
DdeI CTNAG 1 cut(s) 24
DpnI GATC 5 cut(s) 144, 425, 463, 482, 575
DpnII GATC 5 cut(s) 142, 423, 461, 480, 573
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
EciI GGCGGA 1 cut(s) 673
Eco24I GRGCYC 1 cut(s) 279
Eco31I GGTCTC 1 cut(s) 710
Eco47I GGWCC 1 cut(s) 385
Eco88I CYCGRG 1 cut(s) 272
Eco91I GGTNACC 5 cut(s) 527, 602, 623, 644, 665
EcoO65I GGTNACC 5 cut(s) 527, 602, 623, 644, 665
EcoT38I GRGCYC 1 cut(s) 279
FaeI CATG 3 cut(s) 160, 224, 358
FaiI YATR 7 cut(s) 53, 158, 174, 222, 306, 356, 510
FaqI GGGAC 1 cut(s) 483
FatI CATG 3 cut(s) 156, 220, 354
Fnu4HI GCNGC 2 cut(s) 295, 327
FriOI GRGCYC 1 cut(s) 279
Fsp4HI GCNGC 2 cut(s) 295, 327
FspBI CTAG 2 cut(s) 140, 254
GlaI GCGC 3 cut(s) 82, 240, 284
GluI GCNGC 2 cut(s) 295, 327
HaeII RGCGCY 2 cut(s) 84, 242
HgaI GACGC 4 cut(s) 140, 523, 627, 648
HhaI GCGC 3 cut(s) 83, 241, 285
Hin1I GRCGYC 3 cut(s) 132, 638, 659
Hin1II CATG 3 cut(s) 160, 224, 358
Hin6I GCGC 3 cut(s) 81, 239, 283
HinP1I GCGC 3 cut(s) 81, 239, 283
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 8 cut(s) 196, 446, 521, 547, 589, 610, 680, 693
HphI GGTGA 8 cut(s) 106, 475, 521, 596, 610, 617, 638, 659
Hpy166II GTNNAC 3 cut(s) 49, 289, 436
Hpy188I TCNGA 3 cut(s) 88, 147, 552
Hpy188III TCNNGA 3 cut(s) 157, 343, 409
Hpy8I GTNNAC 3 cut(s) 49, 289, 436
Hpy99I CGWCG 3 cut(s) 134, 643, 664
HpyAV CCTTC 2 cut(s) 34, 460
HpyCH4III ACNGT 2 cut(s) 335, 433
HpyCH4V TGCA 1 cut(s) 204
HpyF10VI GCNNNNNNNGC 2 cut(s) 291, 514
HpyF3I CTNAG 1 cut(s) 24
Hsp92I GRCGYC 3 cut(s) 132, 638, 659
Hsp92II CATG 3 cut(s) 160, 224, 358
HspAI GCGC 3 cut(s) 81, 239, 283
KroI GCCGGC 2 cut(s) 634, 655
KroNI GCCGGC 2 cut(s) 636, 657
Kzo9I GATC 5 cut(s) 142, 423, 461, 480, 573
LmnI GCTCC 1 cut(s) 14
Lsp1109I GCAGC 1 cut(s) 338
LweI GCATC 1 cut(s) 306
MaeI CTAG 2 cut(s) 140, 254
MaeIII GTNAC 8 cut(s) 70, 335, 527, 602, 616, 623, 644, 665
MalI GATC 5 cut(s) 144, 425, 463, 482, 575
MboI GATC 5 cut(s) 142, 423, 461, 480, 573
MboII GAAGA 4 cut(s) 40, 172, 377, 433
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 1 cut(s) 279
MluCI AATT 3 cut(s) 89, 161, 169
MlyI GAGTC 4 cut(s) 455, 619, 689, 702
MnlI CCTC 5 cut(s) 24, 94, 194, 281, 380
MreI CGCCGGCG 2 cut(s) 634, 655
MroNI GCCGGC 2 cut(s) 634, 655
MroXI GAANNNNTTC 1 cut(s) 152
MseI TTAA 1 cut(s) 380
MspA1I CMGCKG 1 cut(s) 329
MvnI CGCG 2 cut(s) 265, 285
MwoI GCNNNNNNNGC 2 cut(s) 291, 514
NaeI GCCGGC 2 cut(s) 636, 657
NdeII GATC 5 cut(s) 142, 423, 461, 480, 573
NgoMIV GCCGGC 2 cut(s) 634, 655
NlaIII CATG 3 cut(s) 160, 224, 358
NlaIV GGNNCC 1 cut(s) 387
NmuCI GTSAC 6 cut(s) 527, 602, 616, 623, 644, 665
NspV TTCGAA 2 cut(s) 194, 315
PaeR7I CTCGAG 1 cut(s) 272
PagI TCATGA 1 cut(s) 156
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PdiI GCCGGC 2 cut(s) 636, 657
PdmI GAANNNNTTC 1 cut(s) 152
PfeI GAWTC 4 cut(s) 196, 521, 547, 589
PinAI ACCGGT 4 cut(s) 620, 627, 648, 669
PkrI GCNGC 2 cut(s) 296, 328
PleI GAGTC 4 cut(s) 454, 618, 688, 701
PpsI GAGTC 4 cut(s) 454, 618, 688, 701
PshBI ATTAAT 1 cut(s) 380
PspEI GGTNACC 5 cut(s) 527, 602, 623, 644, 665
PspN4I GGNNCC 1 cut(s) 387
PspPI GGNCC 1 cut(s) 385
PspXI VCTCGAGB 1 cut(s) 272
PsuI RGATCY 1 cut(s) 142
PvuII CAGCTG 1 cut(s) 329
SaqAI TTAA 1 cut(s) 380
SatI GCNGC 2 cut(s) 295, 327
Sau3AI GATC 5 cut(s) 142, 423, 461, 480, 573
Sau96I GGNCC 1 cut(s) 385
SchI GAGTC 4 cut(s) 455, 619, 689, 702
SduI GDGCHC 1 cut(s) 279
SfaNI GCATC 1 cut(s) 306
Sfr274I CTCGAG 1 cut(s) 272
SfuI TTCGAA 2 cut(s) 194, 315
SgrAI CRCCGGYG 2 cut(s) 634, 655
SinI GGWCC 1 cut(s) 385
SlaI CTCGAG 1 cut(s) 272
SmlI CTYRAG 2 cut(s) 272, 407
SmoI CTYRAG 2 cut(s) 272, 407
Sse9I AATT 3 cut(s) 89, 161, 169
SsiI CCGC 2 cut(s) 294, 684
SspMI CTAG 2 cut(s) 140, 254
TaaI ACNGT 2 cut(s) 335, 433
TaqI TCGA 7 cut(s) 194, 273, 300, 315, 363, 464, 572
TaqII GACCGA 1 cut(s) 572
TasI AATT 3 cut(s) 89, 161, 169
TauI GCSGC 1 cut(s) 297
TfiI GAWTC 4 cut(s) 196, 521, 547, 589
Tru1I TTAA 1 cut(s) 380
Tru9I TTAA 1 cut(s) 380
TseFI GTSAC 6 cut(s) 527, 602, 616, 623, 644, 665
TseI GCWGC 1 cut(s) 326
Tsp45I GTSAC 6 cut(s) 527, 602, 616, 623, 644, 665
TspDTI ATGAA 4 cut(s) 53, 145, 173, 237
VpaK11BI GGWCC 1 cut(s) 385
VspI ATTAAT 1 cut(s) 380
XapI RAATTY 2 cut(s) 89, 161
XhoI CTCGAG 1 cut(s) 272
XmnI GAANNNNTTC 1 cut(s) 152
XspI CTAG 2 cut(s) 140, 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.