Rmu_sc0003747.1_g000003

4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003747.1
OG0019960
Physical Location & Seq
Reverse (-)
27289 .. 28992
1704 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003747.1_g000003.1.cds

Sequence Viewer

Length: 921 bp
atggaggtgaagagggtcccgaaagaggaagggaggaagcaatttgaggtagtggacaagggtgatgtggtcattttacctgcctttggagctacagtggatgagatgctcatcttgagtgacaaaaatgttcaaattgtggacactacttgcccttgggtttcaaaggtttggaatactgtagagaagcacaagaaaggggagtacacttcaattattcatggtgtatatgatcatgaggaagctgtagcaactgcttcatatgctggaaaatacgttactgtgaaaaatatggatgaggcatcgtatgtgtgtgactatattcttggtggtgaacttaatgggtctagctcaactaaagaggcatttcaggagaaatttaagaaagcaatttcatcggggttcgatccggacaaagatctggttaaatgtgggattgcaaatcagactacaatgctcatgactgagacagacagcattggaaaactagtagagaatacaatgatgcgcaagtatggagtggagaatatcaatgaccactttgtgagcttcaacacaatatgttatgcgactcaggagcgacaagatgcaatgctcaagctagttgaggaaaagctggattttatattagttgttggagggtggaactcagataacacctctcacctacaagagatggcggaacttcgcggaattccctcgtattggattgatagtgaggagagaataggccctggaaaccgaatagcttataaactcaaacacggcgagttggttgagaacgaaaactggctgcctgagggtcctgtgactattggtgtgacatctggtgcctctactccaaataaggttgttgaagacgcccttatcaaggtgtttgatatcaagcgtaaagaggccctgcaactaggcataatctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00900 ko01100 ko01110 ko01130 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
LYTB

Protein Analysis

306

Amino Acids

34.2

Weight (kDa)

5.05

Isoelectric Point (pI)

27.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019960)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0262401
rosa_multiflora Rmu_sc0003747.1_g000003
rosa_roxburghii Rroxscaffold_177G00434480 Rroxscaffold_7G00205310
rosa_samantha Rh6BG111700 Rh6CG109700 Rh6DG099300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 753
Acc16I TGCGCA 1 cut(s) 509
Acc36I ACCTGC 1 cut(s) 88
AccB1I GGYRCC 1 cut(s) 830
AccII CGCG 1 cut(s) 690
AccIII TCCGGA 1 cut(s) 409
AciI CCGC 2 cut(s) 680, 690
AclWI GGATC 1 cut(s) 401
AcsI RAATTY 2 cut(s) 377, 693
AcyI GRCGYC 1 cut(s) 861
AdeI CACNNNGTG 1 cut(s) 544
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 4 cut(s) 25, 86, 705, 871
AgsI TTSAA 5 cut(s) 134, 165, 213, 553, 857
AhlI ACTAGT 1 cut(s) 487
AjnI CCWGG 1 cut(s) 733
AluBI AGCT 7 cut(s) 92, 245, 351, 549, 601, 616, 749
AluI AGCT 7 cut(s) 92, 245, 351, 549, 601, 616, 749
Alw26I GTCTC 1 cut(s) 461
AlwI GGATC 1 cut(s) 401
Aor13HI TCCGGA 1 cut(s) 409
AoxI GGCC 2 cut(s) 730, 897
ApeKI GCWGC 1 cut(s) 793
ApoI RAATTY 2 cut(s) 377, 693
AspLEI GCGC 1 cut(s) 510
AspS9I GGNCC 4 cut(s) 16, 731, 803, 898
AsuHPI GGTGA 4 cut(s) 19, 74, 344, 656
AvaII GGWCC 2 cut(s) 16, 803
AxyI CCTNAGG 1 cut(s) 798
BanI GGYRCC 1 cut(s) 830
BbsI GAAGAC 1 cut(s) 864
BbvI GCAGC 1 cut(s) 780
BccI CCATC 1 cut(s) 670
BceAI ACGGC 1 cut(s) 781
BcgI CGANNNNNNTGC 2 cut(s) 378, 412
BciT130I CCWGG 1 cut(s) 735
BclI TGATCA 1 cut(s) 232
BcoDI GTCTC 1 cut(s) 461
BcuI ACTAGT 1 cut(s) 487
BfaI CTAG 5 cut(s) 348, 488, 602, 908, 919
BfmI CTRYAG 3 cut(s) 93, 180, 246
BfuAI ACCTGC 1 cut(s) 88
BglII AGATCT 1 cut(s) 418
BisI GCNGC 1 cut(s) 794
BlsI GCNGC 1 cut(s) 795
Bme1390I CCNGG 1 cut(s) 735
Bme18I GGWCC 2 cut(s) 16, 803
BmgT120I GGNCC 4 cut(s) 16, 731, 803, 898
BmiI GGNNCC 4 cut(s) 17, 18, 804, 832
BmrFI CCNGG 1 cut(s) 735
BmsI GCATC 4 cut(s) 96, 311, 495, 577
BpiI GAAGAC 1 cut(s) 864
BpuEI CTTGAG 2 cut(s) 136, 581
BsaBI GATNNNNATC 1 cut(s) 110
BsaHI GRCGYC 1 cut(s) 861
BsaJI CCNNGG 2 cut(s) 155, 733
BsaWI WCCGGW 1 cut(s) 409
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 86, 705, 871
Bse1I ACTGG 1 cut(s) 794
Bse21I CCTNAGG 1 cut(s) 798
Bse3DI GCAATG 1 cut(s) 597
Bse8I GATNNNNATC 1 cut(s) 110
BseAI TCCGGA 1 cut(s) 409
BseBI CCWGG 1 cut(s) 735
BseDI CCNNGG 2 cut(s) 155, 733
BseGI GGATG 2 cut(s) 106, 301
BseJI GATNNNNATC 1 cut(s) 110
BseLI CCNNNNNNNGG 4 cut(s) 25, 86, 705, 871
BseMI GCAATG 1 cut(s) 597
BseMII CTCAG 4 cut(s) 456, 587, 663, 789
BseNI ACTGG 1 cut(s) 794
BseRI GAGGAG 1 cut(s) 734
BseXI GCAGC 1 cut(s) 780
Bsh1236I CGCG 1 cut(s) 690
BshFI GGCC 2 cut(s) 732, 899
BshNI GGYRCC 1 cut(s) 830
BsiSI CCGG 1 cut(s) 410
BslFI GGGAC 1 cut(s) 2
BslI CCNNNNNNNGG 4 cut(s) 25, 86, 705, 871
BsmAI GTCTC 1 cut(s) 461
BsmFI GGGAC 1 cut(s) 2
BsnI GGCC 2 cut(s) 732, 899
Bsp13I TCCGGA 1 cut(s) 409
Bsp143I GATC 3 cut(s) 232, 406, 418
BspACI CCGC 2 cut(s) 680, 690
BspANI GGCC 2 cut(s) 732, 899
BspCNI CTCAG 4 cut(s) 457, 586, 662, 790
BspEI TCCGGA 1 cut(s) 409
BspFNI CGCG 1 cut(s) 690
BspHI TCATGA 2 cut(s) 235, 459
BspLI GGNNCC 4 cut(s) 17, 18, 804, 832
BspMI ACCTGC 1 cut(s) 88
BspPI GGATC 1 cut(s) 401
BspT107I GGYRCC 1 cut(s) 830
BsrDI GCAATG 1 cut(s) 597
BsrI ACTGG 1 cut(s) 794
BssECI CCNNGG 2 cut(s) 155, 733
BssMI GATC 3 cut(s) 232, 406, 418
BssNI GRCGYC 1 cut(s) 861
BssT1I CCWWGG 1 cut(s) 155
Bst2UI CCWGG 1 cut(s) 735
Bst4CI ACNGT 3 cut(s) 97, 181, 283
Bst6I CTCTTC 1 cut(s) 5
BstACI GRCGYC 1 cut(s) 861
BstDEI CTNAG 4 cut(s) 465, 573, 649, 798
BstENI CCTNNNNNAGG 1 cut(s) 869
BstF5I GGATG 2 cut(s) 106, 301
BstFNI CGCG 1 cut(s) 690
BstHHI GCGC 1 cut(s) 510
BstKTI GATC 3 cut(s) 235, 409, 421
BstMAI GTCTC 1 cut(s) 461
BstMBI GATC 3 cut(s) 232, 406, 418
BstMWI GCNNNNNNNGC 2 cut(s) 89, 263
BstNI CCWGG 1 cut(s) 735
BstSCI CCNGG 1 cut(s) 733
BstSFI CTRYAG 3 cut(s) 93, 180, 246
BstUI CGCG 1 cut(s) 690
BstV1I GCAGC 1 cut(s) 780
BstV2I GAAGAC 1 cut(s) 864
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
Bsu36I CCTNAGG 1 cut(s) 798
BsuRI GGCC 2 cut(s) 732, 899
BtsCI GGATG 2 cut(s) 106, 301
BtsIMutI CAGTG 1 cut(s) 102
BveI ACCTGC 1 cut(s) 88
CciI TCATGA 2 cut(s) 235, 459
CfoI GCGC 1 cut(s) 510
Cfr13I GGNCC 4 cut(s) 16, 731, 803, 898
CseI GACGC 1 cut(s) 869
Csp6I GTAC 1 cut(s) 205
CviAII CATG 3 cut(s) 221, 236, 460
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 4 cut(s) 465, 573, 649, 798
DpnI GATC 3 cut(s) 234, 408, 420
DpnII GATC 3 cut(s) 232, 406, 418
DraIII CACNNNGTG 1 cut(s) 544
Eam1104I CTCTTC 1 cut(s) 5
EarI CTCTTC 1 cut(s) 5
EciI GGCGGA 1 cut(s) 695
Eco130I CCWWGG 1 cut(s) 155
Eco32I GATATC 1 cut(s) 883
Eco47I GGWCC 2 cut(s) 16, 803
Eco81I CCTNAGG 1 cut(s) 798
EcoNI CCTNNNNNAGG 1 cut(s) 869
EcoO109I RGGNCCY 4 cut(s) 16, 731, 803, 898
EcoRI GAATTC 1 cut(s) 693
EcoRII CCWGG 1 cut(s) 733
EcoRV GATATC 1 cut(s) 883
EcoT14I CCWWGG 1 cut(s) 155
ErhI CCWWGG 1 cut(s) 155
FaeI CATG 3 cut(s) 224, 239, 463
FalI AAGNNNNNCTT 2 cut(s) 849, 881
FaqI GGGAC 1 cut(s) 2
FatI CATG 3 cut(s) 220, 235, 459
FauNDI CATATG 1 cut(s) 262
FbaI TGATCA 1 cut(s) 232
Fnu4HI GCNGC 1 cut(s) 794
FokI GGATG 2 cut(s) 113, 308
Fsp4HI GCNGC 1 cut(s) 794
FspBI CTAG 5 cut(s) 348, 488, 602, 908, 919
FspI TGCGCA 1 cut(s) 509
GlaI GCGC 1 cut(s) 509
GluI GCNGC 1 cut(s) 794
HaeIII GGCC 2 cut(s) 732, 899
HapII CCGG 1 cut(s) 410
HgaI GACGC 1 cut(s) 869
HhaI GCGC 1 cut(s) 510
Hin1I GRCGYC 1 cut(s) 861
Hin1II CATG 3 cut(s) 224, 239, 463
Hin6I GCGC 1 cut(s) 508
HinP1I GCGC 1 cut(s) 508
HinfI GANTC 1 cut(s) 571
HpaII CCGG 1 cut(s) 410
HphI GGTGA 4 cut(s) 19, 74, 344, 656
Hpy166II GTNNAC 4 cut(s) 55, 142, 207, 335
Hpy188I TCNGA 2 cut(s) 447, 652
Hpy188III TCNNGA 7 cut(s) 19, 115, 236, 371, 410, 460, 575
Hpy8I GTNNAC 4 cut(s) 55, 142, 207, 335
HpyAV CCTTC 1 cut(s) 23
HpyCH4III ACNGT 3 cut(s) 97, 181, 283
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 3 cut(s) 440, 590, 904
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 263
HpyF3I CTNAG 4 cut(s) 465, 573, 649, 798
HpySE526I ACGT 1 cut(s) 276
Hsp92I GRCGYC 1 cut(s) 861
Hsp92II CATG 3 cut(s) 224, 239, 463
HspAI GCGC 1 cut(s) 508
KflI GGGWCCC 1 cut(s) 16
Kpn2I TCCGGA 1 cut(s) 409
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 3 cut(s) 232, 406, 418
LmnI GCTCC 2 cut(s) 89, 577
Lsp1109I GCAGC 1 cut(s) 780
LweI GCATC 4 cut(s) 96, 311, 495, 577
MaeI CTAG 5 cut(s) 348, 488, 602, 908, 919
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 5 cut(s) 119, 277, 314, 808, 820
MalI GATC 3 cut(s) 234, 408, 420
MboI GATC 3 cut(s) 232, 406, 418
MboII GAAGA 2 cut(s) 22, 869
MflI RGATCY 1 cut(s) 418
MluCI AATT 6 cut(s) 41, 135, 213, 377, 390, 693
MlyI GAGTC 1 cut(s) 565
MmeI TCCRAC 1 cut(s) 616
MroI TCCGGA 1 cut(s) 409
MseI TTAA 3 cut(s) 339, 381, 426
MspI CCGG 1 cut(s) 410
MspR9I CCNGG 1 cut(s) 735
MvaI CCWGG 1 cut(s) 735
MvnI CGCG 1 cut(s) 690
MwoI GCNNNNNNNGC 2 cut(s) 89, 263
NdeI CATATG 1 cut(s) 262
NdeII GATC 3 cut(s) 232, 406, 418
NlaIII CATG 3 cut(s) 224, 239, 463
NlaIV GGNNCC 4 cut(s) 17, 18, 804, 832
NmuCI GTSAC 4 cut(s) 119, 314, 808, 820
NsbI TGCGCA 1 cut(s) 509
PagI TCATGA 2 cut(s) 235, 459
PkrI GCNGC 1 cut(s) 795
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
PpuMI RGGWCCY 2 cut(s) 16, 803
PsiI TTATAA 1 cut(s) 753
Psp5II RGGWCCY 2 cut(s) 16, 803
Psp6I CCWGG 1 cut(s) 733
PspGI CCWGG 1 cut(s) 733
PspN4I GGNNCC 4 cut(s) 17, 18, 804, 832
PspPI GGNCC 4 cut(s) 16, 731, 803, 898
PspPPI RGGWCCY 2 cut(s) 16, 803
PsuI RGATCY 1 cut(s) 418
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
SaqAI TTAA 3 cut(s) 339, 381, 426
SatI GCNGC 1 cut(s) 794
Sau3AI GATC 3 cut(s) 232, 406, 418
Sau96I GGNCC 4 cut(s) 16, 731, 803, 898
SchI GAGTC 1 cut(s) 565
ScrFI CCNGG 1 cut(s) 735
SfaNI GCATC 4 cut(s) 96, 311, 495, 577
SfcI CTRYAG 3 cut(s) 93, 180, 246
SinI GGWCC 2 cut(s) 16, 803
SmlI CTYRAG 2 cut(s) 115, 596
SmoI CTYRAG 2 cut(s) 115, 596
SpeI ACTAGT 1 cut(s) 487
Sse9I AATT 6 cut(s) 41, 135, 213, 377, 390, 693
SsiI CCGC 2 cut(s) 680, 690
SspMI CTAG 5 cut(s) 348, 488, 602, 908, 919
StyD4I CCNGG 1 cut(s) 733
StyI CCWWGG 1 cut(s) 155
TaaI ACNGT 3 cut(s) 97, 181, 283
TaiI ACGT 1 cut(s) 279
TaqI TCGA 1 cut(s) 405
TasI AATT 6 cut(s) 41, 135, 213, 377, 390, 693
TatI WGTACW 1 cut(s) 204
Tru1I TTAA 3 cut(s) 339, 381, 426
Tru9I TTAA 3 cut(s) 339, 381, 426
TscAI CASTG 1 cut(s) 102
TseFI GTSAC 4 cut(s) 119, 314, 808, 820
TseI GCWGC 1 cut(s) 793
Tsp45I GTSAC 4 cut(s) 119, 314, 808, 820
TspDTI ATGAA 3 cut(s) 209, 249, 384
TspRI CASTG 1 cut(s) 102
VpaK11BI GGWCC 2 cut(s) 16, 803
XagI CCTNNNNNAGG 1 cut(s) 869
XapI RAATTY 2 cut(s) 377, 693
XspI CTAG 5 cut(s) 348, 488, 602, 908, 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.