Rh6BG111700

4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0019960
Physical Location & Seq
Forward (+)
17458434 .. 17482995
24562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG111700.1

Sequence Viewer

Length: 945 bp
ATGGTGAATAAGCTGTTGGACGAGATGGAGGTGAAGAGGGTCCCGAAAGAGGAAGGGAGGAAGCAATTTGAGGTAGTGGACAAGGGTGATGTGGTCATTTTACCTGCTTTTGGAGCTACAGTGGATGAGATGCTCATCTTGAGTGACAAAAATGTTCAAATTGTGGACACTACCTGCCCTTGGGTTTCAAAGGTTTGGAAAACTGTAGAGAAGCACAAGAAAGGGGAGTACACTTCAATTATTCATGGTGTATATGATCATGAGGAAGCTGTAGCAACTGCTTCATATGCTGGAAAATACGTTACTGTGAAAAATATGGATGAGGCATCGTATGTGTGTGACTATATTCTTGGTGGTGAACTTAATGGGTCTAGCTCAACTAAAGAGGCATTTCAGGAGAAATTTAAGAAAGCAATTTCATCGGGGTTCGATCCGGACAAAGATCTGGTTAAATGTGGGATTGCAAATCAGACTACAATGCTCATGACTGAGACAGACAGCATTGGAAAACTAGTAGAGAATACAATGATGCGCAAGTATGGAGTGGAGAATATCAATGACCACTTTGTGAGCTTCAACACAATATGTTATGCGACTCAGGAGCGACAAGATGCAATGCTCAAGCTAGTTGAGGAAAAGCTGAATTTTATATTAGTTGTTGGAGGGTGGAACTCAGATAACACCTCTCACCTAAAAGAGATGGCGGAACTTCGCGGAATTCCCTCTTATTGGATTGATAGTGAGGAGAGAATAGGCCCTGGAAACCGAATAGCTTATAAACTCAAACACGGCGAGTTGGTTGAGAACGAAAACTGGCTGCCTGAGGGTCCTGTGACTATTGGTGTGACATCTGGTGCCTCTACTCCAAATAAGGTTGTTGAAGACGCCCTTATCAAGGTGTTTGATATCAAGCGTAAAGAGGCCCTGCAACTAGGCATAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00900 ko01100 ko01110 ko01130 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
LYTB

Protein Analysis

314

Amino Acids

35.15

Weight (kDa)

5.2

Isoelectric Point (pI)

25.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LYTB PF02401 7 - 297 1.7e-57 LytB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019960)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0262401
rosa_multiflora Rmu_sc0003747.1_g000003
rosa_roxburghii Rroxscaffold_177G00434480 Rroxscaffold_7G00205310
rosa_samantha Rh6BG111700 Rh6CG109700 Rh6DG099300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 777
Acc16I TGCGCA 1 cut(s) 533
Acc36I ACCTGC 2 cut(s) 112, 182
AccB1I GGYRCC 1 cut(s) 854
AccII CGCG 1 cut(s) 714
AccIII TCCGGA 1 cut(s) 433
AciI CCGC 2 cut(s) 704, 714
AclWI GGATC 1 cut(s) 425
AcsI RAATTY 3 cut(s) 401, 643, 717
AcyI GRCGYC 1 cut(s) 885
AdeI CACNNNGTG 1 cut(s) 568
AfaI GTAC 1 cut(s) 230
AfiI CCNNNNNNNGG 5 cut(s) 49, 110, 180, 729, 895
AgsI TTSAA 5 cut(s) 158, 189, 237, 577, 881
AhlI ACTAGT 1 cut(s) 511
AjnI CCWGG 1 cut(s) 757
AjuI GAANNNNNNNTTGG 1 cut(s) 31
AluBI AGCT 8 cut(s) 13, 116, 269, 375, 573, 625, 640, 773
AluI AGCT 8 cut(s) 13, 116, 269, 375, 573, 625, 640, 773
Alw26I GTCTC 1 cut(s) 485
AlwI GGATC 1 cut(s) 425
Aor13HI TCCGGA 1 cut(s) 433
AoxI GGCC 2 cut(s) 754, 921
ApeKI GCWGC 1 cut(s) 817
ApoI RAATTY 3 cut(s) 401, 643, 717
AspLEI GCGC 1 cut(s) 534
AspS9I GGNCC 4 cut(s) 40, 755, 827, 922
AsuHPI GGTGA 5 cut(s) 16, 43, 98, 368, 680
AvaII GGWCC 2 cut(s) 40, 827
AxyI CCTNAGG 1 cut(s) 822
BanI GGYRCC 1 cut(s) 854
BbsI GAAGAC 1 cut(s) 888
BbvI GCAGC 1 cut(s) 804
BccI CCATC 2 cut(s) 19, 694
BceAI ACGGC 1 cut(s) 805
BcgI CGANNNNNNTGC 2 cut(s) 402, 436
BciT130I CCWGG 1 cut(s) 759
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 1 cut(s) 485
BcuI ACTAGT 1 cut(s) 511
BfaI CTAG 4 cut(s) 372, 512, 626, 932
BfmI CTRYAG 3 cut(s) 117, 204, 270
BfuAI ACCTGC 2 cut(s) 112, 182
BglII AGATCT 1 cut(s) 442
BisI GCNGC 1 cut(s) 818
BlsI GCNGC 1 cut(s) 819
Bme1390I CCNGG 1 cut(s) 759
Bme18I GGWCC 2 cut(s) 40, 827
BmgT120I GGNCC 4 cut(s) 40, 755, 827, 922
BmiI GGNNCC 4 cut(s) 41, 42, 828, 856
BmrFI CCNGG 1 cut(s) 759
BmsI GCATC 4 cut(s) 120, 335, 519, 601
BpiI GAAGAC 1 cut(s) 888
BpuEI CTTGAG 2 cut(s) 160, 605
BsaBI GATNNNNATC 1 cut(s) 134
BsaHI GRCGYC 1 cut(s) 885
BsaJI CCNNGG 2 cut(s) 179, 757
BsaWI WCCGGW 1 cut(s) 433
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bsc4I CCNNNNNNNGG 5 cut(s) 49, 110, 180, 729, 895
Bse1I ACTGG 1 cut(s) 818
Bse21I CCTNAGG 1 cut(s) 822
Bse3DI GCAATG 1 cut(s) 621
Bse8I GATNNNNATC 1 cut(s) 134
BseAI TCCGGA 1 cut(s) 433
BseBI CCWGG 1 cut(s) 759
BseDI CCNNGG 2 cut(s) 179, 757
BseGI GGATG 2 cut(s) 130, 325
BseJI GATNNNNATC 1 cut(s) 134
BseLI CCNNNNNNNGG 5 cut(s) 49, 110, 180, 729, 895
BseMI GCAATG 1 cut(s) 621
BseMII CTCAG 4 cut(s) 480, 611, 687, 813
BseNI ACTGG 1 cut(s) 818
BseRI GAGGAG 1 cut(s) 758
BseXI GCAGC 1 cut(s) 804
Bsh1236I CGCG 1 cut(s) 714
BshFI GGCC 2 cut(s) 756, 923
BshNI GGYRCC 1 cut(s) 854
BsiSI CCGG 1 cut(s) 434
BslFI GGGAC 1 cut(s) 26
BslI CCNNNNNNNGG 5 cut(s) 49, 110, 180, 729, 895
BsmAI GTCTC 1 cut(s) 485
BsmFI GGGAC 1 cut(s) 26
BsnI GGCC 2 cut(s) 756, 923
Bsp13I TCCGGA 1 cut(s) 433
Bsp143I GATC 3 cut(s) 256, 430, 442
BspACI CCGC 2 cut(s) 704, 714
BspANI GGCC 2 cut(s) 756, 923
BspCNI CTCAG 4 cut(s) 481, 610, 686, 814
BspEI TCCGGA 1 cut(s) 433
BspFNI CGCG 1 cut(s) 714
BspHI TCATGA 2 cut(s) 259, 483
BspLI GGNNCC 4 cut(s) 41, 42, 828, 856
BspMI ACCTGC 2 cut(s) 112, 182
BspPI GGATC 1 cut(s) 425
BspT107I GGYRCC 1 cut(s) 854
BsrDI GCAATG 1 cut(s) 621
BsrI ACTGG 1 cut(s) 818
BssECI CCNNGG 2 cut(s) 179, 757
BssMI GATC 3 cut(s) 256, 430, 442
BssNI GRCGYC 1 cut(s) 885
BssT1I CCWWGG 1 cut(s) 179
Bst2UI CCWGG 1 cut(s) 759
Bst4CI ACNGT 3 cut(s) 121, 205, 307
Bst6I CTCTTC 1 cut(s) 29
BstACI GRCGYC 1 cut(s) 885
BstDEI CTNAG 4 cut(s) 489, 597, 673, 822
BstENI CCTNNNNNAGG 1 cut(s) 893
BstF5I GGATG 2 cut(s) 130, 325
BstFNI CGCG 1 cut(s) 714
BstHHI GCGC 1 cut(s) 534
BstKTI GATC 3 cut(s) 259, 433, 445
BstMAI GTCTC 1 cut(s) 485
BstMBI GATC 3 cut(s) 256, 430, 442
BstMWI GCNNNNNNNGC 2 cut(s) 113, 287
BstNI CCWGG 1 cut(s) 759
BstSCI CCNGG 1 cut(s) 757
BstSFI CTRYAG 3 cut(s) 117, 204, 270
BstUI CGCG 1 cut(s) 714
BstV1I GCAGC 1 cut(s) 804
BstV2I GAAGAC 1 cut(s) 888
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
Bsu36I CCTNAGG 1 cut(s) 822
BsuRI GGCC 2 cut(s) 756, 923
BtsCI GGATG 2 cut(s) 130, 325
BtsIMutI CAGTG 1 cut(s) 126
BveI ACCTGC 2 cut(s) 112, 182
CciI TCATGA 2 cut(s) 259, 483
CfoI GCGC 1 cut(s) 534
Cfr13I GGNCC 4 cut(s) 40, 755, 827, 922
CseI GACGC 1 cut(s) 893
Csp6I GTAC 1 cut(s) 229
CviAII CATG 3 cut(s) 245, 260, 484
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 4 cut(s) 489, 597, 673, 822
DpnI GATC 3 cut(s) 258, 432, 444
DpnII GATC 3 cut(s) 256, 430, 442
DraIII CACNNNGTG 1 cut(s) 568
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
EciI GGCGGA 1 cut(s) 719
Eco130I CCWWGG 1 cut(s) 179
Eco32I GATATC 1 cut(s) 907
Eco47I GGWCC 2 cut(s) 40, 827
Eco81I CCTNAGG 1 cut(s) 822
EcoNI CCTNNNNNAGG 1 cut(s) 893
EcoO109I RGGNCCY 4 cut(s) 40, 755, 827, 922
EcoRI GAATTC 1 cut(s) 717
EcoRII CCWGG 1 cut(s) 757
EcoRV GATATC 1 cut(s) 907
EcoT14I CCWWGG 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 179
FaeI CATG 3 cut(s) 248, 263, 487
FalI AAGNNNNNCTT 2 cut(s) 873, 905
FaqI GGGAC 1 cut(s) 26
FatI CATG 3 cut(s) 244, 259, 483
FauNDI CATATG 1 cut(s) 286
FbaI TGATCA 1 cut(s) 256
Fnu4HI GCNGC 1 cut(s) 818
FokI GGATG 2 cut(s) 137, 332
Fsp4HI GCNGC 1 cut(s) 818
FspBI CTAG 4 cut(s) 372, 512, 626, 932
FspI TGCGCA 1 cut(s) 533
GlaI GCGC 1 cut(s) 533
GluI GCNGC 1 cut(s) 818
HaeIII GGCC 2 cut(s) 756, 923
HapII CCGG 1 cut(s) 434
HgaI GACGC 1 cut(s) 893
HhaI GCGC 1 cut(s) 534
Hin1I GRCGYC 1 cut(s) 885
Hin1II CATG 3 cut(s) 248, 263, 487
Hin6I GCGC 1 cut(s) 532
HinP1I GCGC 1 cut(s) 532
HinfI GANTC 1 cut(s) 595
HpaII CCGG 1 cut(s) 434
HphI GGTGA 5 cut(s) 16, 43, 98, 368, 680
Hpy166II GTNNAC 4 cut(s) 79, 166, 231, 359
Hpy188I TCNGA 2 cut(s) 471, 676
Hpy188III TCNNGA 7 cut(s) 43, 139, 260, 395, 434, 484, 599
Hpy8I GTNNAC 4 cut(s) 79, 166, 231, 359
HpyAV CCTTC 1 cut(s) 47
HpyCH4III ACNGT 3 cut(s) 121, 205, 307
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 3 cut(s) 464, 614, 928
HpyF10VI GCNNNNNNNGC 2 cut(s) 113, 287
HpyF3I CTNAG 4 cut(s) 489, 597, 673, 822
HpySE526I ACGT 1 cut(s) 300
Hsp92I GRCGYC 1 cut(s) 885
Hsp92II CATG 3 cut(s) 248, 263, 487
HspAI GCGC 1 cut(s) 532
KflI GGGWCCC 1 cut(s) 40
Kpn2I TCCGGA 1 cut(s) 433
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 3 cut(s) 256, 430, 442
LmnI GCTCC 2 cut(s) 113, 601
Lsp1109I GCAGC 1 cut(s) 804
LweI GCATC 4 cut(s) 120, 335, 519, 601
MaeI CTAG 4 cut(s) 372, 512, 626, 932
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 5 cut(s) 143, 301, 338, 832, 844
MalI GATC 3 cut(s) 258, 432, 444
MboI GATC 3 cut(s) 256, 430, 442
MboII GAAGA 2 cut(s) 46, 893
MflI RGATCY 1 cut(s) 442
MluCI AATT 8 cut(s) 65, 159, 237, 401, 414, 643, 717, 939
MlyI GAGTC 1 cut(s) 589
MmeI TCCRAC 1 cut(s) 640
MroI TCCGGA 1 cut(s) 433
MseI TTAA 3 cut(s) 363, 405, 450
MspI CCGG 1 cut(s) 434
MspR9I CCNGG 1 cut(s) 759
MvaI CCWGG 1 cut(s) 759
MvnI CGCG 1 cut(s) 714
MwoI GCNNNNNNNGC 2 cut(s) 113, 287
NdeI CATATG 1 cut(s) 286
NdeII GATC 3 cut(s) 256, 430, 442
NlaIII CATG 3 cut(s) 248, 263, 487
NlaIV GGNNCC 4 cut(s) 41, 42, 828, 856
NmuCI GTSAC 4 cut(s) 143, 338, 832, 844
NsbI TGCGCA 1 cut(s) 533
PagI TCATGA 2 cut(s) 259, 483
PkrI GCNGC 1 cut(s) 819
PleI GAGTC 1 cut(s) 589
PpsI GAGTC 1 cut(s) 589
PpuMI RGGWCCY 2 cut(s) 40, 827
PsiI TTATAA 1 cut(s) 777
Psp5II RGGWCCY 2 cut(s) 40, 827
Psp6I CCWGG 1 cut(s) 757
PspGI CCWGG 1 cut(s) 757
PspN4I GGNNCC 4 cut(s) 41, 42, 828, 856
PspPI GGNCC 4 cut(s) 40, 755, 827, 922
PspPPI RGGWCCY 2 cut(s) 40, 827
PsuI RGATCY 1 cut(s) 442
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
SaqAI TTAA 3 cut(s) 363, 405, 450
SatI GCNGC 1 cut(s) 818
Sau3AI GATC 3 cut(s) 256, 430, 442
Sau96I GGNCC 4 cut(s) 40, 755, 827, 922
SchI GAGTC 1 cut(s) 589
ScrFI CCNGG 1 cut(s) 759
SfaNI GCATC 4 cut(s) 120, 335, 519, 601
SfcI CTRYAG 3 cut(s) 117, 204, 270
SinI GGWCC 2 cut(s) 40, 827
SmlI CTYRAG 2 cut(s) 139, 620
SmoI CTYRAG 2 cut(s) 139, 620
SpeI ACTAGT 1 cut(s) 511
Sse9I AATT 8 cut(s) 65, 159, 237, 401, 414, 643, 717, 939
SsiI CCGC 2 cut(s) 704, 714
SspMI CTAG 4 cut(s) 372, 512, 626, 932
StyD4I CCNGG 1 cut(s) 757
StyI CCWWGG 1 cut(s) 179
TaaI ACNGT 3 cut(s) 121, 205, 307
TaiI ACGT 1 cut(s) 303
TaqI TCGA 1 cut(s) 429
TasI AATT 8 cut(s) 65, 159, 237, 401, 414, 643, 717, 939
TatI WGTACW 1 cut(s) 228
Tru1I TTAA 3 cut(s) 363, 405, 450
Tru9I TTAA 3 cut(s) 363, 405, 450
TscAI CASTG 1 cut(s) 126
TseFI GTSAC 4 cut(s) 143, 338, 832, 844
TseI GCWGC 1 cut(s) 817
Tsp45I GTSAC 4 cut(s) 143, 338, 832, 844
TspDTI ATGAA 3 cut(s) 233, 273, 408
TspRI CASTG 1 cut(s) 126
VpaK11BI GGWCC 2 cut(s) 40, 827
XagI CCTNNNNNAGG 1 cut(s) 893
XapI RAATTY 3 cut(s) 401, 643, 717
XspI CTAG 4 cut(s) 372, 512, 626, 932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.