Rmu_sc0004191.1_g000025

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004191.1
OG0025550
Physical Location & Seq
Reverse (-)
92833 .. 95943
3111 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004191.1_g000025.1.cds

Sequence Viewer

Length: 711 bp
atgatcaggctcgacactgatcgcgatcggtcaccgaaagggctgtctttggccaacctctttgcctgggtttgggcctcgcagaaccaactttgtcttccacacacggcagcaccctaccttccacctcactgctgcacagacatacagcccccgcgcggctcctctcacgcaaaagcgcctgcatcttcgcgcagaacagcctccgcccaggccctcgatcccgttactgttgctccccatccccgcgcagcacagccccgagcttgtccaccggtgccgcccttgaaaacccaccaatctcaacctcgacccgcctgcagcaccagaccagatccggccgagaagaagaaaggagaacctttgaagacacccaaagcactgtgggatgcccttaaaggatgttttgggaacattcacgatactttgctcccaggactggctgttaaatggaatggaatccgcttgcttgactataaaaaggtcaatgacttcaacaaggacatgttgctcttaaaggcacgtctcaatttctgtggaagggaaatcacagaagatgatatgatctacaagactcttaccccatttcctacttcagcttttatactagtcaaccagtatcagttggagtatgacaacaaaagaatcacaaccttcaacaagttgataagcctactgcaagtgctgagagacaaaatgagattctcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 zf-CCHC

Protein Analysis

236

Amino Acids

26.5

Weight (kDa)

9.66

Isoelectric Point (pI)

51.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025550)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003022.1_g000003 Rmu_sc0004191.1_g000025 Rmu_sc0004569.1_g000003

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 277
AccII CGCG 5 cut(s) 24, 157, 159, 193, 249
AciI CCGC 7 cut(s) 155, 159, 207, 247, 281, 315, 463
AclWI GGATC 2 cut(s) 215, 329
AcoI YGGCCR 2 cut(s) 51, 339
AcuI CTGAAG 1 cut(s) 579
AfiI CCNNNNNNNGG 3 cut(s) 72, 158, 439
AflIII ACRYGT 1 cut(s) 504
AgeI ACCGGT 1 cut(s) 274
AgsI TTSAA 4 cut(s) 289, 367, 496, 658
AhlI ACTAGT 1 cut(s) 607
AjiI CACGTC 1 cut(s) 524
AjnI CCWGG 3 cut(s) 65, 210, 433
AjuI GAANNNNNNNTTGG 2 cut(s) 81, 113
AluBI AGCT 2 cut(s) 266, 599
AluI AGCT 2 cut(s) 266, 599
Alw26I GTCTC 2 cut(s) 530, 684
AlwI GGATC 2 cut(s) 215, 329
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 4 cut(s) 51, 75, 213, 339
ApeKI GCWGC 4 cut(s) 110, 135, 251, 321
ArsI GACNNNNNNTTYG 2 cut(s) 29, 61
AsiGI ACCGGT 1 cut(s) 274
AspLEI GCGC 4 cut(s) 159, 181, 195, 251
AspS9I GGNCC 2 cut(s) 75, 214
AsuHPI GGTGA 1 cut(s) 24
AvaI CYCGRG 1 cut(s) 261
BalI TGGCCA 1 cut(s) 53
BanI GGYRCC 1 cut(s) 277
BbsI GAAGAC 2 cut(s) 89, 374
BbvI GCAGC 4 cut(s) 122, 122, 263, 333
BccI CCATC 1 cut(s) 249
BceAI ACGGC 1 cut(s) 123
BcgI CGANNNNNNTGC 2 cut(s) 300, 334
BciT130I CCWGG 3 cut(s) 67, 212, 435
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 2 cut(s) 530, 684
BcuI ACTAGT 1 cut(s) 607
BfaI CTAG 1 cut(s) 608
BfmI CTRYAG 1 cut(s) 319
BfoI RGCGCY 1 cut(s) 182
BisI GCNGC 6 cut(s) 111, 136, 160, 252, 281, 322
BlsI GCNGC 6 cut(s) 112, 137, 161, 253, 282, 323
Bme1390I CCNGG 3 cut(s) 67, 212, 435
BmeT110I CYCGRG 1 cut(s) 261
BmgBI CACGTC 1 cut(s) 524
BmgT120I GGNCC 2 cut(s) 75, 214
BmiI GGNNCC 2 cut(s) 163, 279
BmrFI CCNGG 3 cut(s) 67, 212, 435
BmsI GCATC 2 cut(s) 194, 379
BpiI GAAGAC 2 cut(s) 89, 374
BsaBI GATNNNNATC 1 cut(s) 24
BsaJI CCNNGG 3 cut(s) 66, 210, 433
BsaWI WCCGGW 1 cut(s) 274
BsaXI ACNNNNNCTCC 2 cut(s) 220, 250
Bsc4I CCNNNNNNNGG 3 cut(s) 72, 158, 439
Bse118I RCCGGY 1 cut(s) 274
Bse1I ACTGG 2 cut(s) 444, 616
Bse8I GATNNNNATC 1 cut(s) 24
BseBI CCWGG 3 cut(s) 67, 212, 435
BseDI CCNNGG 3 cut(s) 66, 210, 433
BseGI GGATG 3 cut(s) 241, 394, 407
BseJI GATNNNNATC 1 cut(s) 24
BseLI CCNNNNNNNGG 3 cut(s) 72, 158, 439
BseMII CTCAG 1 cut(s) 677
BseNI ACTGG 2 cut(s) 444, 616
BseRI GAGGAG 1 cut(s) 154
BseX3I CGGCCG 1 cut(s) 339
BseXI GCAGC 4 cut(s) 122, 122, 263, 333
BsgI GTGCAG 1 cut(s) 121
Bsh1236I CGCG 5 cut(s) 24, 157, 159, 193, 249
Bsh1285I CGRYCG 2 cut(s) 28, 342
BshFI GGCC 4 cut(s) 53, 77, 215, 341
BshNI GGYRCC 1 cut(s) 277
BshTI ACCGGT 1 cut(s) 274
BsiEI CGRYCG 2 cut(s) 28, 342
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 2 cut(s) 275, 338
BslI CCNNNNNNNGG 3 cut(s) 72, 158, 439
BsmAI GTCTC 2 cut(s) 530, 684
BsmBI CGTCTC 1 cut(s) 530
BsnI GGCC 4 cut(s) 53, 77, 215, 341
BsoBI CYCGRG 1 cut(s) 261
Bsp143I GATC 6 cut(s) 3, 19, 25, 220, 334, 564
Bsp68I TCGCGA 1 cut(s) 24
BspACI CCGC 7 cut(s) 155, 159, 207, 247, 281, 315, 463
BspANI GGCC 4 cut(s) 53, 77, 215, 341
BspCNI CTCAG 1 cut(s) 678
BspFNI CGCG 5 cut(s) 24, 157, 159, 193, 249
BspLI GGNNCC 2 cut(s) 163, 279
BspMAI CTGCAG 1 cut(s) 323
BspPI GGATC 2 cut(s) 215, 329
BspT107I GGYRCC 1 cut(s) 277
BsrFI RCCGGY 1 cut(s) 274
BsrI ACTGG 2 cut(s) 444, 616
BssAI RCCGGY 1 cut(s) 274
BssECI CCNNGG 3 cut(s) 66, 210, 433
BssMI GATC 6 cut(s) 3, 19, 25, 220, 334, 564
Bst2UI CCWGG 3 cut(s) 67, 212, 435
Bst4CI ACNGT 2 cut(s) 232, 384
BstC8I GCNNGC 3 cut(s) 183, 319, 467
BstDEI CTNAG 1 cut(s) 686
BstEII GGTNACC 1 cut(s) 30
BstF5I GGATG 3 cut(s) 241, 394, 407
BstFNI CGCG 5 cut(s) 24, 157, 159, 193, 249
BstH2I RGCGCY 1 cut(s) 182
BstHHI GCGC 4 cut(s) 159, 181, 195, 251
BstKTI GATC 6 cut(s) 6, 22, 28, 223, 337, 567
BstMAI GTCTC 2 cut(s) 530, 684
BstMBI GATC 6 cut(s) 3, 19, 25, 220, 334, 564
BstMCI CGRYCG 2 cut(s) 28, 342
BstNI CCWGG 3 cut(s) 67, 212, 435
BstNSI RCATGY 1 cut(s) 508
BstPI GGTNACC 1 cut(s) 30
BstSCI CCNGG 3 cut(s) 65, 210, 433
BstSFI CTRYAG 1 cut(s) 319
BstUI CGCG 5 cut(s) 24, 157, 159, 193, 249
BstV1I GCAGC 4 cut(s) 122, 122, 263, 333
BstV2I GAAGAC 2 cut(s) 89, 374
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BstZI CGGCCG 1 cut(s) 339
BsuRI GGCC 4 cut(s) 53, 77, 215, 341
BtrI CACGTC 1 cut(s) 524
BtsCI GGATG 3 cut(s) 241, 394, 407
BtsI GCAGTG 1 cut(s) 130
BtsIMutI CAGTG 3 cut(s) 15, 130, 380
BtuMI TCGCGA 1 cut(s) 24
Cac8I GCNNGC 3 cut(s) 183, 319, 467
CfoI GCGC 4 cut(s) 159, 181, 195, 251
Cfr10I RCCGGY 1 cut(s) 274
Cfr13I GGNCC 2 cut(s) 75, 214
CspAI ACCGGT 1 cut(s) 274
CspCI CAANNNNNGTGG 2 cut(s) 517, 552
CviAII CATG 1 cut(s) 505
DdeI CTNAG 1 cut(s) 686
DpnI GATC 6 cut(s) 5, 21, 27, 222, 336, 566
DpnII GATC 6 cut(s) 3, 19, 25, 220, 334, 564
EaeI YGGCCR 2 cut(s) 51, 339
EagI CGGCCG 1 cut(s) 339
EciI GGCGGA 1 cut(s) 196
EclXI CGGCCG 1 cut(s) 339
Eco52I CGGCCG 1 cut(s) 339
Eco57I CTGAAG 1 cut(s) 579
Eco88I CYCGRG 1 cut(s) 261
Eco91I GGTNACC 1 cut(s) 30
EcoO109I RGGNCCY 1 cut(s) 214
EcoO65I GGTNACC 1 cut(s) 30
EcoRII CCWGG 3 cut(s) 65, 210, 433
Esp3I CGTCTC 1 cut(s) 530
FaeI CATG 1 cut(s) 508
FaiI YATR 6 cut(s) 146, 477, 506, 563, 605, 633
FatI CATG 1 cut(s) 504
FauI CCCGC 3 cut(s) 162, 254, 322
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 6 cut(s) 111, 136, 160, 252, 281, 322
FokI GGATG 3 cut(s) 228, 401, 414
Fsp4HI GCNGC 6 cut(s) 111, 136, 160, 252, 281, 322
FspBI CTAG 1 cut(s) 608
GlaI GCGC 4 cut(s) 158, 180, 194, 250
GluI GCNGC 6 cut(s) 111, 136, 160, 252, 281, 322
HaeII RGCGCY 1 cut(s) 182
HaeIII GGCC 4 cut(s) 53, 77, 215, 341
HapII CCGG 2 cut(s) 275, 338
HhaI GCGC 4 cut(s) 159, 181, 195, 251
Hin1II CATG 1 cut(s) 508
Hin6I GCGC 4 cut(s) 157, 179, 193, 249
HinP1I GCGC 4 cut(s) 157, 179, 193, 249
HincII GTYRAC 1 cut(s) 613
HindII GTYRAC 1 cut(s) 613
HinfI GANTC 4 cut(s) 459, 574, 645, 702
HpaII CCGG 2 cut(s) 275, 338
HphI GGTGA 1 cut(s) 24
Hpy166II GTNNAC 2 cut(s) 272, 613
Hpy188III TCNNGA 3 cut(s) 23, 419, 708
Hpy8I GTNNAC 2 cut(s) 272, 613
HpyAV CCTTC 3 cut(s) 131, 534, 664
HpyCH4III ACNGT 2 cut(s) 232, 384
HpyCH4IV ACGT 1 cut(s) 523
HpyCH4V TGCA 4 cut(s) 138, 185, 321, 679
HpyF3I CTNAG 1 cut(s) 686
HpySE526I ACGT 1 cut(s) 523
Hsp92II CATG 1 cut(s) 508
HspAI GCGC 4 cut(s) 157, 179, 193, 249
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 6 cut(s) 3, 19, 25, 220, 334, 564
LmnI GCTCC 3 cut(s) 167, 241, 435
Lsp1109I GCAGC 4 cut(s) 122, 122, 263, 333
LweI GCATC 2 cut(s) 194, 379
MaeI CTAG 1 cut(s) 608
MaeII ACGT 1 cut(s) 523
MaeIII GTNAC 2 cut(s) 30, 226
MalI GATC 6 cut(s) 5, 21, 27, 222, 336, 566
MboI GATC 6 cut(s) 3, 19, 25, 220, 334, 564
MboII GAAGA 6 cut(s) 89, 180, 358, 361, 379, 566
MflI RGATCY 1 cut(s) 334
MlsI TGGCCA 1 cut(s) 53
MluCI AATT 1 cut(s) 529
MluNI TGGCCA 1 cut(s) 53
MlyI GAGTC 1 cut(s) 568
MmeI TCCRAC 1 cut(s) 606
MnlI CCTC 7 cut(s) 68, 88, 138, 175, 214, 227, 318
Mox20I TGGCCA 1 cut(s) 53
MscI TGGCCA 1 cut(s) 53
MseI TTAA 3 cut(s) 396, 447, 515
Msp20I TGGCCA 1 cut(s) 53
MspI CCGG 2 cut(s) 275, 338
MspR9I CCNGG 3 cut(s) 67, 212, 435
MvaI CCWGG 3 cut(s) 67, 212, 435
MvnI CGCG 5 cut(s) 24, 157, 159, 193, 249
NdeII GATC 6 cut(s) 3, 19, 25, 220, 334, 564
NlaIII CATG 1 cut(s) 508
NlaIV GGNNCC 2 cut(s) 163, 279
NmeAIII GCCGAG 1 cut(s) 367
NmuCI GTSAC 1 cut(s) 30
NruI TCGCGA 1 cut(s) 24
NspI RCATGY 1 cut(s) 508
PciI ACATGT 1 cut(s) 504
PfeI GAWTC 3 cut(s) 459, 645, 702
PinAI ACCGGT 1 cut(s) 274
PkrI GCNGC 6 cut(s) 112, 137, 161, 253, 282, 323
Ple19I CGATCG 1 cut(s) 28
PleI GAGTC 1 cut(s) 568
PpsI GAGTC 1 cut(s) 568
PscI ACATGT 1 cut(s) 504
Psp6I CCWGG 3 cut(s) 65, 210, 433
PspEI GGTNACC 1 cut(s) 30
PspGI CCWGG 3 cut(s) 65, 210, 433
PspN4I GGNNCC 2 cut(s) 163, 279
PspPI GGNCC 2 cut(s) 75, 214
PstI CTGCAG 1 cut(s) 323
PsuI RGATCY 1 cut(s) 334
PvuI CGATCG 1 cut(s) 28
RruI TCGCGA 1 cut(s) 24
SaqAI TTAA 3 cut(s) 396, 447, 515
SatI GCNGC 6 cut(s) 111, 136, 160, 252, 281, 322
Sau3AI GATC 6 cut(s) 3, 19, 25, 220, 334, 564
Sau96I GGNCC 2 cut(s) 75, 214
SchI GAGTC 1 cut(s) 568
ScrFI CCNGG 3 cut(s) 67, 212, 435
SfaNI GCATC 2 cut(s) 194, 379
SfcI CTRYAG 1 cut(s) 319
SgrAI CRCCGGYG 1 cut(s) 274
SpeI ACTAGT 1 cut(s) 607
Sse9I AATT 1 cut(s) 529
SsiI CCGC 7 cut(s) 155, 159, 207, 247, 281, 315, 463
SspMI CTAG 1 cut(s) 608
StyD4I CCNGG 3 cut(s) 65, 210, 433
TaaI ACNGT 2 cut(s) 232, 384
TaiI ACGT 1 cut(s) 526
TaqI TCGA 3 cut(s) 12, 219, 310
TaqII GACCGA 1 cut(s) 18
TasI AATT 1 cut(s) 529
TauI GCSGC 2 cut(s) 162, 283
TfiI GAWTC 3 cut(s) 459, 645, 702
Tru1I TTAA 3 cut(s) 396, 447, 515
Tru9I TTAA 3 cut(s) 396, 447, 515
TscAI CASTG 3 cut(s) 22, 137, 387
TseFI GTSAC 1 cut(s) 30
TseI GCWGC 4 cut(s) 110, 135, 251, 321
Tsp45I GTSAC 1 cut(s) 30
TspRI CASTG 3 cut(s) 22, 137, 387
XceI RCATGY 1 cut(s) 508
XspI CTAG 1 cut(s) 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.