Rmu_sc0004316.1_g000004

RRP12-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004316.1
OG0025581
Physical Location & Seq
Reverse (-)
13847 .. 14254
408 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 408 bp
atgtccgggatattccgagaccacaaggcacctccgaggccattggcctacttgggcgccacgtgtacctctctcgacatagccgccgtctccaagcctcaagcttccggtcacgtgctcgacgcctacctcacgattctgtccatcgtcatcccaaaagtccctgctgcgtttctgatcaagaatctagggctggtggttaattccttagcgcgacctttcctgtcttcgtctccgattgacgagagtaccagctgcttagtagtgaaatgcatagctgaattgatcattgccatcacaaacgtcgtcaacaattggtcagatgtttcggaattgtacatcttcttcctcagcttcgctaccgttgacagccgcaagggatcttattatgttctttcaattgattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUC173

Protein Analysis

135

Amino Acids

14.55

Weight (kDa)

6.7

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_RRP12_N PF25772 1 - 125 1.2e-24 RRP12-like, N-terminal HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025581)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004316.1_g000004
rosa_roxburghii Rroxscaffold_2G00146710
rosa_samantha Rh2DG096000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 28, 56
AccII CGCG 1 cut(s) 214
AciI CCGC 2 cut(s) 84, 373
AclWI GGATC 1 cut(s) 388
AcvI CACGTG 2 cut(s) 63, 115
AcyI GRCGYC 2 cut(s) 57, 123
AfaI GTAC 3 cut(s) 67, 250, 338
AflIII ACRYGT 1 cut(s) 62
AgsI TTSAA 1 cut(s) 399
AluBI AGCT 4 cut(s) 104, 255, 278, 354
AluI AGCT 4 cut(s) 104, 255, 278, 354
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 3 cut(s) 12, 94, 237
AlwI GGATC 1 cut(s) 388
AoxI GGCC 2 cut(s) 38, 45
ApeKI GCWGC 2 cut(s) 167, 255
AspLEI GCGC 2 cut(s) 59, 214
AsuC2I CCSGG 1 cut(s) 7
BaeI ACNNNNGTAYC 2 cut(s) 232, 265
BanI GGYRCC 2 cut(s) 28, 56
BarI GAAGNNNNNNTAC 2 cut(s) 329, 361
BbrPI CACGTG 2 cut(s) 63, 115
BbsI GAAGAC 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 120
BbvCI CCTCAGC 1 cut(s) 350
BbvI GCAGC 2 cut(s) 154, 242
BccI CCATC 2 cut(s) 152, 302
BceAI ACGGC 1 cut(s) 71
BclI TGATCA 2 cut(s) 177, 285
BcnI CCSGG 1 cut(s) 7
BcoDI GTCTC 3 cut(s) 12, 94, 237
BfaI CTAG 1 cut(s) 188
BfoI RGCGCY 1 cut(s) 60
BisI GCNGC 4 cut(s) 84, 168, 256, 373
BlsI GCNGC 4 cut(s) 85, 169, 257, 374
Bme1390I CCNGG 1 cut(s) 7
BmiI GGNNCC 2 cut(s) 30, 58
BmrFI CCNGG 1 cut(s) 7
BpiI GAAGAC 1 cut(s) 219
Bpu10I CCTNAGC 2 cut(s) 208, 350
BpuEI CTTGAG 1 cut(s) 84
BpuMI CCSGG 1 cut(s) 7
BsaAI YACGTR 2 cut(s) 63, 115
BsaHI GRCGYC 2 cut(s) 57, 123
BsaI GGTCTC 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 35
BsaWI WCCGGW 1 cut(s) 107
Bse3DI GCAATG 1 cut(s) 288
BseDI CCNNGG 1 cut(s) 35
BseGI GGATG 1 cut(s) 150
BseMI GCAATG 1 cut(s) 288
BseMII CTCAG 1 cut(s) 364
BseXI GCAGC 2 cut(s) 154, 242
Bsh1236I CGCG 1 cut(s) 214
BshFI GGCC 2 cut(s) 40, 47
BshNI GGYRCC 2 cut(s) 28, 56
BsiHKAI GWGCWC 1 cut(s) 120
BsiSI CCGG 2 cut(s) 6, 108
BslFI GGGAC 1 cut(s) 146
BsmAI GTCTC 3 cut(s) 12, 94, 237
BsmBI CGTCTC 2 cut(s) 94, 237
BsmFI GGGAC 1 cut(s) 146
BsnI GGCC 2 cut(s) 40, 47
Bso31I GGTCTC 1 cut(s) 12
Bsp1286I GDGCHC 1 cut(s) 120
Bsp1407I TGTACA 1 cut(s) 336
Bsp143I GATC 3 cut(s) 177, 285, 380
BspACI CCGC 2 cut(s) 84, 373
BspANI GGCC 2 cut(s) 40, 47
BspCNI CTCAG 1 cut(s) 363
BspFNI CGCG 1 cut(s) 214
BspLI GGNNCC 2 cut(s) 30, 58
BspPI GGATC 1 cut(s) 388
BspT107I GGYRCC 2 cut(s) 28, 56
BspTNI GGTCTC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 288
BsrGI TGTACA 1 cut(s) 336
BssECI CCNNGG 1 cut(s) 35
BssMI GATC 3 cut(s) 177, 285, 380
BssNI GRCGYC 2 cut(s) 57, 123
Bst4CI ACNGT 1 cut(s) 364
BstACI GRCGYC 2 cut(s) 57, 123
BstAUI TGTACA 1 cut(s) 336
BstBAI YACGTR 2 cut(s) 63, 115
BstDEI CTNAG 3 cut(s) 208, 259, 350
BstF5I GGATG 1 cut(s) 150
BstFNI CGCG 1 cut(s) 214
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 2 cut(s) 59, 214
BstKTI GATC 3 cut(s) 180, 288, 383
BstMAI GTCTC 3 cut(s) 12, 94, 237
BstMBI GATC 3 cut(s) 177, 285, 380
BstSCI CCNGG 1 cut(s) 5
BstUI CGCG 1 cut(s) 214
BstV1I GCAGC 2 cut(s) 154, 242
BstV2I GAAGAC 1 cut(s) 219
BstX2I RGATCY 1 cut(s) 380
BstYI RGATCY 1 cut(s) 380
BsuRI GGCC 2 cut(s) 40, 47
BtsCI GGATG 1 cut(s) 150
CfoI GCGC 2 cut(s) 59, 214
CseI GACGC 1 cut(s) 131
Csp6I GTAC 3 cut(s) 66, 249, 337
CviQI GTAC 3 cut(s) 66, 249, 337
DdeI CTNAG 3 cut(s) 208, 259, 350
DinI GGCGCC 1 cut(s) 58
DpnI GATC 3 cut(s) 179, 287, 382
DpnII GATC 3 cut(s) 177, 285, 380
Eco31I GGTCTC 1 cut(s) 12
Eco72I CACGTG 2 cut(s) 63, 115
EcoT22I ATGCAT 1 cut(s) 275
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
Esp3I CGTCTC 2 cut(s) 94, 237
FaiI YATR 3 cut(s) 80, 275, 390
FaqI GGGAC 1 cut(s) 146
FbaI TGATCA 2 cut(s) 177, 285
Fnu4HI GCNGC 4 cut(s) 84, 168, 256, 373
FokI GGATG 1 cut(s) 137
Fsp4HI GCNGC 4 cut(s) 84, 168, 256, 373
FspBI CTAG 1 cut(s) 188
GlaI GCGC 2 cut(s) 58, 213
GluI GCNGC 4 cut(s) 84, 168, 256, 373
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 2 cut(s) 40, 47
HapII CCGG 2 cut(s) 6, 108
HgaI GACGC 1 cut(s) 131
HhaI GCGC 2 cut(s) 59, 214
Hin1I GRCGYC 2 cut(s) 57, 123
Hin6I GCGC 2 cut(s) 57, 212
HinP1I GCGC 2 cut(s) 57, 212
HincII GTYRAC 2 cut(s) 310, 367
HindII GTYRAC 2 cut(s) 310, 367
HindIII AAGCTT 1 cut(s) 102
HinfI GANTC 2 cut(s) 136, 184
HpaII CCGG 2 cut(s) 6, 108
Hpy166II GTNNAC 3 cut(s) 66, 310, 367
Hpy188I TCNGA 6 cut(s) 17, 36, 177, 237, 322, 331
Hpy188III TCNNGA 3 cut(s) 74, 133, 181
Hpy8I GTNNAC 3 cut(s) 66, 310, 367
Hpy99I CGWCG 2 cut(s) 125, 308
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4IV ACGT 3 cut(s) 62, 114, 303
HpyCH4V TGCA 1 cut(s) 273
HpyF3I CTNAG 3 cut(s) 208, 259, 350
HpySE526I ACGT 3 cut(s) 62, 114, 303
Hsp92I GRCGYC 2 cut(s) 57, 123
HspAI GCGC 2 cut(s) 57, 212
KasI GGCGCC 1 cut(s) 56
Ksp22I TGATCA 2 cut(s) 177, 285
Kzo9I GATC 3 cut(s) 177, 285, 380
LpnPI CCDG 6 cut(s) 19, 121, 177, 179, 236, 265
Lsp1109I GCAGC 2 cut(s) 154, 242
MaeI CTAG 1 cut(s) 188
MaeII ACGT 3 cut(s) 62, 114, 303
MaeIII GTNAC 1 cut(s) 110
MalI GATC 3 cut(s) 179, 287, 382
MboI GATC 3 cut(s) 177, 285, 380
MboII GAAGA 3 cut(s) 219, 334, 337
MfeI CAATTG 2 cut(s) 313, 399
MflI RGATCY 1 cut(s) 380
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 5 cut(s) 202, 281, 313, 332, 399
Mly113I GGCGCC 1 cut(s) 57
MnlI CCTC 6 cut(s) 30, 42, 79, 108, 140, 359
Mph1103I ATGCAT 1 cut(s) 275
MseI TTAA 1 cut(s) 201
MspA1I CMGCKG 1 cut(s) 255
MspI CCGG 2 cut(s) 6, 108
MspR9I CCNGG 1 cut(s) 7
MunI CAATTG 2 cut(s) 313, 399
MvnI CGCG 1 cut(s) 214
NarI GGCGCC 1 cut(s) 57
NciI CCSGG 1 cut(s) 7
NdeII GATC 3 cut(s) 177, 285, 380
NlaIV GGNNCC 2 cut(s) 30, 58
NmuCI GTSAC 1 cut(s) 110
NsiI ATGCAT 1 cut(s) 275
PfeI GAWTC 2 cut(s) 136, 184
PfoI TCCNGGA 1 cut(s) 5
PkrI GCNGC 4 cut(s) 85, 169, 257, 374
PluTI GGCGCC 1 cut(s) 60
PmaCI CACGTG 2 cut(s) 63, 115
PmlI CACGTG 2 cut(s) 63, 115
Ppu21I YACGTR 2 cut(s) 63, 115
PspCI CACGTG 2 cut(s) 63, 115
PspN4I GGNNCC 2 cut(s) 30, 58
PsuI RGATCY 1 cut(s) 380
PvuII CAGCTG 1 cut(s) 255
RsaI GTAC 3 cut(s) 67, 250, 338
RsaNI GTAC 3 cut(s) 66, 249, 337
SaqAI TTAA 1 cut(s) 201
SatI GCNGC 4 cut(s) 84, 168, 256, 373
Sau3AI GATC 3 cut(s) 177, 285, 380
ScrFI CCNGG 1 cut(s) 7
SduI GDGCHC 1 cut(s) 120
SfoI GGCGCC 1 cut(s) 58
SmlI CTYRAG 1 cut(s) 99
SmoI CTYRAG 1 cut(s) 99
Sse9I AATT 5 cut(s) 202, 281, 313, 332, 399
SsiI CCGC 2 cut(s) 84, 373
SspDI GGCGCC 1 cut(s) 56
SspMI CTAG 1 cut(s) 188
StyD4I CCNGG 1 cut(s) 5
TaaI ACNGT 1 cut(s) 364
TaiI ACGT 3 cut(s) 65, 117, 306
TaqI TCGA 2 cut(s) 75, 120
TasI AATT 5 cut(s) 202, 281, 313, 332, 399
TatI WGTACW 1 cut(s) 336
TauI GCSGC 2 cut(s) 86, 375
TfiI GAWTC 2 cut(s) 136, 184
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 2 cut(s) 167, 255
Tsp45I GTSAC 1 cut(s) 110
XspI CTAG 1 cut(s) 188
Zsp2I ATGCAT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.