Rh2DG096000

RRP12-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0025581
Physical Location & Seq
Forward (+)
7529054 .. 7529326
273 bp
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UTR
Exon/CDS
Intron
Rh2DG096000.1

Sequence Viewer

Length: 273 bp
ATGTCCGGGATATTCCGAGACCACAAGGCACCTCCGACGCCATTGGCCTACTTGGGCGCCACGTGTACCTCTCTCGACATAGCCGCCGCCTCTAAGCCTCAAGCTTCCGGTCTCGTGCTCGACGCCTACCTCACGATTCTGTCCATCGTCATCCCAAAAGTCCCTGCTGCGTTTCTGATCAAGAATCTAGGGCTGGTGGTTAATTCCTTAGCGCGACCTTTCCTGTCTTCGTCTCCGATTGACAAGAGTACCAGCTGCTTAGTAGTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUC173

Protein Analysis

90

Amino Acids

9.37

Weight (kDa)

9.39

Isoelectric Point (pI)

45.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_RRP12_N PF25772 1 - 83 6.4e-16 RRP12-like, N-terminal HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0025581)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004316.1_g000004
rosa_roxburghii Rroxscaffold_2G00146710
rosa_samantha Rh2DG096000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 28, 56
AccII CGCG 1 cut(s) 214
AciI CCGC 2 cut(s) 84, 87
AcvI CACGTG 1 cut(s) 63
AcyI GRCGYC 3 cut(s) 38, 57, 123
AfaI GTAC 2 cut(s) 67, 250
AflIII ACRYGT 1 cut(s) 62
AluBI AGCT 2 cut(s) 104, 255
AluI AGCT 2 cut(s) 104, 255
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 3 cut(s) 12, 116, 237
AoxI GGCC 1 cut(s) 45
ApeKI GCWGC 2 cut(s) 167, 255
AspLEI GCGC 2 cut(s) 59, 214
AsuC2I CCSGG 1 cut(s) 7
BaeI ACNNNNGTAYC 2 cut(s) 232, 265
BanI GGYRCC 2 cut(s) 28, 56
BauI CACGAG 1 cut(s) 113
BbrPI CACGTG 1 cut(s) 63
BbsI GAAGAC 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 2 cut(s) 154, 242
BccI CCATC 1 cut(s) 152
BclI TGATCA 1 cut(s) 177
BcnI CCSGG 1 cut(s) 7
BcoDI GTCTC 3 cut(s) 12, 116, 237
BfaI CTAG 1 cut(s) 188
BfoI RGCGCY 1 cut(s) 60
BisI GCNGC 4 cut(s) 84, 87, 168, 256
BlsI GCNGC 4 cut(s) 85, 88, 169, 257
Bme1390I CCNGG 1 cut(s) 7
BmiI GGNNCC 2 cut(s) 30, 58
BmrFI CCNGG 1 cut(s) 7
BpiI GAAGAC 1 cut(s) 219
Bpu10I CCTNAGC 1 cut(s) 208
BpuEI CTTGAG 1 cut(s) 84
BpuMI CCSGG 1 cut(s) 7
BsaAI YACGTR 1 cut(s) 63
BsaHI GRCGYC 3 cut(s) 38, 57, 123
BsaI GGTCTC 2 cut(s) 12, 116
BsaWI WCCGGW 1 cut(s) 107
BseGI GGATG 1 cut(s) 150
BseXI GCAGC 2 cut(s) 154, 242
Bsh1236I CGCG 1 cut(s) 214
BshFI GGCC 1 cut(s) 47
BshNI GGYRCC 2 cut(s) 28, 56
BsiHKAI GWGCWC 1 cut(s) 120
BsiSI CCGG 2 cut(s) 6, 108
BslFI GGGAC 1 cut(s) 146
BsmAI GTCTC 3 cut(s) 12, 116, 237
BsmBI CGTCTC 1 cut(s) 237
BsmFI GGGAC 1 cut(s) 146
BsnI GGCC 1 cut(s) 47
Bso31I GGTCTC 2 cut(s) 12, 116
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 1 cut(s) 177
BspACI CCGC 2 cut(s) 84, 87
BspANI GGCC 1 cut(s) 47
BspFNI CGCG 1 cut(s) 214
BspLI GGNNCC 2 cut(s) 30, 58
BspT107I GGYRCC 2 cut(s) 28, 56
BspTNI GGTCTC 2 cut(s) 12, 116
BssMI GATC 1 cut(s) 177
BssNI GRCGYC 3 cut(s) 38, 57, 123
BssSI CACGAG 1 cut(s) 113
Bst2BI CACGAG 1 cut(s) 113
BstACI GRCGYC 3 cut(s) 38, 57, 123
BstBAI YACGTR 1 cut(s) 63
BstDEI CTNAG 3 cut(s) 93, 208, 259
BstF5I GGATG 1 cut(s) 150
BstFNI CGCG 1 cut(s) 214
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 2 cut(s) 59, 214
BstKTI GATC 1 cut(s) 180
BstMAI GTCTC 3 cut(s) 12, 116, 237
BstMBI GATC 1 cut(s) 177
BstSCI CCNGG 1 cut(s) 5
BstUI CGCG 1 cut(s) 214
BstV1I GCAGC 2 cut(s) 154, 242
BstV2I GAAGAC 1 cut(s) 219
BsuRI GGCC 1 cut(s) 47
BtsCI GGATG 1 cut(s) 150
CfoI GCGC 2 cut(s) 59, 214
CseI GACGC 2 cut(s) 46, 131
Csp6I GTAC 2 cut(s) 66, 249
CviJI RGCY 6 cut(s) 47, 83, 97, 104, 193, 255
CviKI_1 RGCY 6 cut(s) 47, 83, 97, 104, 193, 255
CviQI GTAC 2 cut(s) 66, 249
DdeI CTNAG 3 cut(s) 93, 208, 259
DinI GGCGCC 1 cut(s) 58
DpnI GATC 1 cut(s) 179
DpnII GATC 1 cut(s) 177
Eco31I GGTCTC 2 cut(s) 12, 116
Eco72I CACGTG 1 cut(s) 63
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
Esp3I CGTCTC 1 cut(s) 237
FaiI YATR 1 cut(s) 80
FaqI GGGAC 1 cut(s) 146
FbaI TGATCA 1 cut(s) 177
Fnu4HI GCNGC 4 cut(s) 84, 87, 168, 256
FokI GGATG 1 cut(s) 137
Fsp4HI GCNGC 4 cut(s) 84, 87, 168, 256
FspBI CTAG 1 cut(s) 188
GlaI GCGC 2 cut(s) 58, 213
GluI GCNGC 4 cut(s) 84, 87, 168, 256
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 1 cut(s) 47
HapII CCGG 2 cut(s) 6, 108
HgaI GACGC 2 cut(s) 46, 131
HhaI GCGC 2 cut(s) 59, 214
Hin1I GRCGYC 3 cut(s) 38, 57, 123
Hin6I GCGC 2 cut(s) 57, 212
HinP1I GCGC 2 cut(s) 57, 212
HindIII AAGCTT 1 cut(s) 102
HinfI GANTC 2 cut(s) 136, 184
HpaII CCGG 2 cut(s) 6, 108
Hpy166II GTNNAC 1 cut(s) 66
Hpy188I TCNGA 4 cut(s) 17, 36, 177, 237
Hpy188III TCNNGA 3 cut(s) 74, 133, 181
Hpy8I GTNNAC 1 cut(s) 66
Hpy99I CGWCG 2 cut(s) 40, 125
HpyCH4IV ACGT 1 cut(s) 62
HpyF3I CTNAG 3 cut(s) 93, 208, 259
HpySE526I ACGT 1 cut(s) 62
Hsp92I GRCGYC 3 cut(s) 38, 57, 123
HspAI GCGC 2 cut(s) 57, 212
KasI GGCGCC 1 cut(s) 56
Ksp22I TGATCA 1 cut(s) 177
Kzo9I GATC 1 cut(s) 177
LpnPI CCDG 6 cut(s) 19, 121, 177, 179, 236, 265
Lsp1109I GCAGC 2 cut(s) 154, 242
MaeI CTAG 1 cut(s) 188
MaeII ACGT 1 cut(s) 62
MalI GATC 1 cut(s) 179
MboI GATC 1 cut(s) 177
MboII GAAGA 1 cut(s) 219
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 1 cut(s) 202
Mly113I GGCGCC 1 cut(s) 57
MmeI TCCRAC 1 cut(s) 59
MnlI CCTC 5 cut(s) 42, 79, 100, 108, 140
MseI TTAA 1 cut(s) 201
MspA1I CMGCKG 1 cut(s) 255
MspI CCGG 2 cut(s) 6, 108
MspR9I CCNGG 1 cut(s) 7
MvnI CGCG 1 cut(s) 214
NarI GGCGCC 1 cut(s) 57
NciI CCSGG 1 cut(s) 7
NdeII GATC 1 cut(s) 177
NlaIV GGNNCC 2 cut(s) 30, 58
PfeI GAWTC 2 cut(s) 136, 184
PfoI TCCNGGA 1 cut(s) 5
PkrI GCNGC 4 cut(s) 85, 88, 169, 257
PluTI GGCGCC 1 cut(s) 60
PmaCI CACGTG 1 cut(s) 63
PmlI CACGTG 1 cut(s) 63
Ppu21I YACGTR 1 cut(s) 63
PspCI CACGTG 1 cut(s) 63
PspN4I GGNNCC 2 cut(s) 30, 58
PvuII CAGCTG 1 cut(s) 255
RsaI GTAC 2 cut(s) 67, 250
RsaNI GTAC 2 cut(s) 66, 249
SaqAI TTAA 1 cut(s) 201
SatI GCNGC 4 cut(s) 84, 87, 168, 256
Sau3AI GATC 1 cut(s) 177
ScrFI CCNGG 1 cut(s) 7
SduI GDGCHC 1 cut(s) 120
SetI ASST 7 cut(s) 34, 65, 71, 106, 132, 220, 257
SfoI GGCGCC 1 cut(s) 58
SmlI CTYRAG 1 cut(s) 99
SmoI CTYRAG 1 cut(s) 99
Sse9I AATT 1 cut(s) 202
SsiI CCGC 2 cut(s) 84, 87
SspDI GGCGCC 1 cut(s) 56
SspMI CTAG 1 cut(s) 188
StyD4I CCNGG 1 cut(s) 5
TaiI ACGT 1 cut(s) 65
TaqI TCGA 2 cut(s) 75, 120
TasI AATT 1 cut(s) 202
TauI GCSGC 2 cut(s) 86, 89
TfiI GAWTC 2 cut(s) 136, 184
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TseI GCWGC 2 cut(s) 167, 255
XspI CTAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.