Rroxscaffold_2G00146710

RRP12-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0025581
Physical Location & Seq
Reverse (-)
84578882 .. 84579277
396 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_2G00146710.1

Sequence Viewer

Length: 396 bp
ATGTCCGGGGTATTCCGAGACCACAAGGCACCTCCGGCGCCGGTGGACTACTTGGGGGCCACGTGCACCTCTCTCGACATCGCCACCGCCTCCATGCCCCAAGCTTCCGGTCACGTGCTCGACGCCCACCTCACGATTCTGTCCATCGTCATCCCAAAAGTCCCTGCTGCGTTTCTGATCAAGAATCTATGGCCTCTGTTTAATTCTATAGCACGACCTCTCCGGTCTTCGTCTCTGATTGACGAGAGTACCGGCTGCTTAGTAGTGAAATGCATAGCTGAATTCATCATTGCCGTCGCAAAAGTCGTCAACAATTGGTCAGATGTTTCAGAACTGTACAGCTTCTTCCTCAGCTTGGCTACCATTAAAGCCCTCGAGGTATCTTATTATTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUC173

Protein Analysis

131

Amino Acids

14.14

Weight (kDa)

6.25

Isoelectric Point (pI)

42.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_RRP12_N PF25772 1 - 121 2.1e-22 RRP12-like, N-terminal HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025581)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004316.1_g000004
rosa_roxburghii Rroxscaffold_2G00146710
rosa_samantha Rh2DG096000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 374
AccB1I GGYRCC 2 cut(s) 28, 37
AciI CCGC 1 cut(s) 87
AcsI RAATTY 1 cut(s) 281
AcvI CACGTG 2 cut(s) 63, 115
AcyI GRCGYC 2 cut(s) 38, 123
AfaI GTAC 2 cut(s) 250, 338
AfiI CCNNNNNNNGG 1 cut(s) 355
AluBI AGCT 4 cut(s) 104, 278, 342, 354
AluI AGCT 4 cut(s) 104, 278, 342, 354
Alw21I GWGCWC 2 cut(s) 68, 120
Alw26I GTCTC 2 cut(s) 12, 237
Alw44I GTGCAC 1 cut(s) 64
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 2 cut(s) 57, 191
ApaLI GTGCAC 1 cut(s) 64
ApeKI GCWGC 2 cut(s) 167, 255
ApoI RAATTY 1 cut(s) 281
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 1 cut(s) 57
AsuC2I CCSGG 1 cut(s) 7
AvaI CYCGRG 1 cut(s) 374
BaeGI GKGCMC 1 cut(s) 68
BaeI ACNNNNGTAYC 2 cut(s) 232, 265
BanI GGYRCC 2 cut(s) 28, 37
BarI GAAGNNNNNNTAC 2 cut(s) 329, 361
BbrPI CACGTG 2 cut(s) 63, 115
BbsI GAAGAC 1 cut(s) 219
Bbv12I GWGCWC 2 cut(s) 68, 120
BbvCI CCTCAGC 1 cut(s) 350
BbvI GCAGC 2 cut(s) 154, 242
BccI CCATC 1 cut(s) 152
BceAI ACGGC 1 cut(s) 278
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BclI TGATCA 1 cut(s) 177
BcnI CCSGG 1 cut(s) 7
BcoDI GTCTC 2 cut(s) 12, 237
BfmI CTRYAG 1 cut(s) 207
BfoI RGCGCY 1 cut(s) 41
BisI GCNGC 2 cut(s) 168, 256
BlsI GCNGC 2 cut(s) 169, 257
Bme1390I CCNGG 1 cut(s) 7
BmeT110I CYCGRG 1 cut(s) 374
BmgT120I GGNCC 1 cut(s) 57
BmiI GGNNCC 3 cut(s) 30, 39, 58
BmrFI CCNGG 1 cut(s) 7
BpiI GAAGAC 1 cut(s) 219
Bpu10I CCTNAGC 1 cut(s) 350
BpuMI CCSGG 1 cut(s) 7
BsaAI YACGTR 2 cut(s) 63, 115
BsaHI GRCGYC 2 cut(s) 38, 123
BsaI GGTCTC 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 6
BsaWI WCCGGW 2 cut(s) 107, 222
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bsc4I CCNNNNNNNGG 1 cut(s) 355
Bse118I RCCGGY 2 cut(s) 40, 251
Bse3DI GCAATG 1 cut(s) 288
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 1 cut(s) 150
BseLI CCNNNNNNNGG 1 cut(s) 355
BseMI GCAATG 1 cut(s) 288
BseMII CTCAG 1 cut(s) 364
BseSI GKGCMC 1 cut(s) 68
BseXI GCAGC 2 cut(s) 154, 242
BshFI GGCC 2 cut(s) 59, 193
BshNI GGYRCC 2 cut(s) 28, 37
BsiHKAI GWGCWC 2 cut(s) 68, 120
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 6 cut(s) 6, 35, 41, 108, 223, 252
BslFI GGGAC 1 cut(s) 146
BslI CCNNNNNNNGG 1 cut(s) 355
BsmAI GTCTC 2 cut(s) 12, 237
BsmBI CGTCTC 1 cut(s) 237
BsmFI GGGAC 1 cut(s) 146
BsnI GGCC 2 cut(s) 59, 193
Bso31I GGTCTC 1 cut(s) 12
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 2 cut(s) 68, 120
Bsp1407I TGTACA 1 cut(s) 336
Bsp143I GATC 1 cut(s) 177
BspACI CCGC 1 cut(s) 87
BspANI GGCC 2 cut(s) 59, 193
BspCNI CTCAG 1 cut(s) 363
BspLI GGNNCC 3 cut(s) 30, 39, 58
BspT107I GGYRCC 2 cut(s) 28, 37
BspTNI GGTCTC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 288
BsrFI RCCGGY 2 cut(s) 40, 251
BsrGI TGTACA 1 cut(s) 336
BssAI RCCGGY 2 cut(s) 40, 251
BssECI CCNNGG 1 cut(s) 6
BssMI GATC 1 cut(s) 177
BssNI GRCGYC 2 cut(s) 38, 123
Bst4CI ACNGT 1 cut(s) 336
BstACI GRCGYC 2 cut(s) 38, 123
BstAUI TGTACA 1 cut(s) 336
BstBAI YACGTR 2 cut(s) 63, 115
BstDEI CTNAG 2 cut(s) 259, 350
BstF5I GGATG 1 cut(s) 150
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 1 cut(s) 180
BstMAI GTCTC 2 cut(s) 12, 237
BstMBI GATC 1 cut(s) 177
BstMWI GCNNNNNNNGC 1 cut(s) 35
BstSCI CCNGG 1 cut(s) 5
BstSFI CTRYAG 1 cut(s) 207
BstSLI GKGCMC 1 cut(s) 68
BstV1I GCAGC 2 cut(s) 154, 242
BstV2I GAAGAC 1 cut(s) 219
BsuRI GGCC 2 cut(s) 59, 193
BtgZI GCGATG 1 cut(s) 64
BtsCI GGATG 1 cut(s) 150
CfoI GCGC 1 cut(s) 40
Cfr10I RCCGGY 2 cut(s) 40, 251
Cfr13I GGNCC 1 cut(s) 57
CseI GACGC 1 cut(s) 131
Csp6I GTAC 2 cut(s) 249, 337
CviAII CATG 1 cut(s) 94
CviJI RGCY 9 cut(s) 59, 104, 193, 255, 278, 342, 354, 359, 371
CviKI_1 RGCY 9 cut(s) 59, 104, 193, 255, 278, 342, 354, 359, 371
CviQI GTAC 2 cut(s) 249, 337
DdeI CTNAG 2 cut(s) 259, 350
DinI GGCGCC 1 cut(s) 39
DpnI GATC 1 cut(s) 179
DpnII GATC 1 cut(s) 177
Eco31I GGTCTC 1 cut(s) 12
Eco72I CACGTG 2 cut(s) 63, 115
Eco88I CYCGRG 1 cut(s) 374
EcoRI GAATTC 1 cut(s) 281
EcoT22I ATGCAT 1 cut(s) 275
EgeI GGCGCC 1 cut(s) 39
EheI GGCGCC 1 cut(s) 39
Esp3I CGTCTC 1 cut(s) 237
FaeI CATG 1 cut(s) 97
FaiI YATR 4 cut(s) 95, 190, 209, 275
FaqI GGGAC 1 cut(s) 146
FatI CATG 1 cut(s) 93
FbaI TGATCA 1 cut(s) 177
Fnu4HI GCNGC 2 cut(s) 168, 256
FokI GGATG 1 cut(s) 137
Fsp4HI GCNGC 2 cut(s) 168, 256
GlaI GCGC 1 cut(s) 39
GluI GCNGC 2 cut(s) 168, 256
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 2 cut(s) 59, 193
HapII CCGG 6 cut(s) 6, 35, 41, 108, 223, 252
HgaI GACGC 1 cut(s) 131
HhaI GCGC 1 cut(s) 40
Hin1I GRCGYC 2 cut(s) 38, 123
Hin1II CATG 1 cut(s) 97
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HindIII AAGCTT 1 cut(s) 102
HinfI GANTC 2 cut(s) 136, 184
HpaII CCGG 6 cut(s) 6, 35, 41, 108, 223, 252
Hpy166II GTNNAC 3 cut(s) 46, 66, 310
Hpy188I TCNGA 5 cut(s) 17, 177, 237, 322, 331
Hpy188III TCNNGA 4 cut(s) 74, 133, 181, 393
Hpy8I GTNNAC 3 cut(s) 46, 66, 310
Hpy99I CGWCG 2 cut(s) 125, 299
HpyCH4III ACNGT 1 cut(s) 336
HpyCH4IV ACGT 2 cut(s) 62, 114
HpyCH4V TGCA 2 cut(s) 66, 273
HpyF10VI GCNNNNNNNGC 1 cut(s) 35
HpyF3I CTNAG 2 cut(s) 259, 350
HpySE526I ACGT 2 cut(s) 62, 114
Hsp92I GRCGYC 2 cut(s) 38, 123
Hsp92II CATG 1 cut(s) 97
HspAI GCGC 1 cut(s) 38
KasI GGCGCC 1 cut(s) 37
Ksp22I TGATCA 1 cut(s) 177
Kzo9I GATC 1 cut(s) 177
LpnPI CCDG 7 cut(s) 19, 48, 54, 121, 177, 236, 265
Lsp1109I GCAGC 2 cut(s) 154, 242
MaeII ACGT 2 cut(s) 62, 114
MaeIII GTNAC 1 cut(s) 110
MalI GATC 1 cut(s) 179
MboI GATC 1 cut(s) 177
MboII GAAGA 2 cut(s) 219, 337
MfeI CAATTG 1 cut(s) 313
MhlI GDGCHC 2 cut(s) 68, 120
MluCI AATT 3 cut(s) 202, 281, 313
Mly113I GGCGCC 1 cut(s) 38
MnlI CCTC 9 cut(s) 42, 79, 100, 140, 204, 228, 359, 370, 383
Mph1103I ATGCAT 1 cut(s) 275
MseI TTAA 2 cut(s) 201, 366
MspI CCGG 6 cut(s) 6, 35, 41, 108, 223, 252
MspR9I CCNGG 1 cut(s) 7
MunI CAATTG 1 cut(s) 313
MwoI GCNNNNNNNGC 1 cut(s) 35
NarI GGCGCC 1 cut(s) 38
NciI CCSGG 1 cut(s) 7
NdeII GATC 1 cut(s) 177
NlaIII CATG 1 cut(s) 97
NlaIV GGNNCC 3 cut(s) 30, 39, 58
NmuCI GTSAC 1 cut(s) 110
NsiI ATGCAT 1 cut(s) 275
PaeR7I CTCGAG 1 cut(s) 374
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PfeI GAWTC 2 cut(s) 136, 184
PkrI GCNGC 2 cut(s) 169, 257
PluTI GGCGCC 1 cut(s) 41
PmaCI CACGTG 2 cut(s) 63, 115
PmlI CACGTG 2 cut(s) 63, 115
Ppu21I YACGTR 2 cut(s) 63, 115
PspCI CACGTG 2 cut(s) 63, 115
PspN4I GGNNCC 3 cut(s) 30, 39, 58
PspPI GGNCC 1 cut(s) 57
PspXI VCTCGAGB 1 cut(s) 374
RsaI GTAC 2 cut(s) 250, 338
RsaNI GTAC 2 cut(s) 249, 337
SaqAI TTAA 2 cut(s) 201, 366
SatI GCNGC 2 cut(s) 168, 256
Sau3AI GATC 1 cut(s) 177
Sau96I GGNCC 1 cut(s) 57
ScrFI CCNGG 1 cut(s) 7
SduI GDGCHC 2 cut(s) 68, 120
SfcI CTRYAG 1 cut(s) 207
SfoI GGCGCC 1 cut(s) 39
Sfr274I CTCGAG 1 cut(s) 374
SgrAI CRCCGGYG 1 cut(s) 40
SlaI CTCGAG 1 cut(s) 374
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
Sse9I AATT 3 cut(s) 202, 281, 313
SsiI CCGC 1 cut(s) 87
SspDI GGCGCC 1 cut(s) 37
StyD4I CCNGG 1 cut(s) 5
TaaI ACNGT 1 cut(s) 336
TaiI ACGT 2 cut(s) 65, 117
TaqI TCGA 3 cut(s) 75, 120, 375
TasI AATT 3 cut(s) 202, 281, 313
TatI WGTACW 1 cut(s) 336
TfiI GAWTC 2 cut(s) 136, 184
Tru1I TTAA 2 cut(s) 201, 366
Tru9I TTAA 2 cut(s) 201, 366
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 2 cut(s) 167, 255
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 1 cut(s) 274
VneI GTGCAC 1 cut(s) 64
XapI RAATTY 1 cut(s) 281
XhoI CTCGAG 1 cut(s) 374
Zsp2I ATGCAT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.