Rmu_sc0004343.1_g000003

Golgi vesicle prefusion complex stabilization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004343.1
OG0031300
Physical Location & Seq
Forward (+)
5777 .. 7520
1744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004343.1_g000003.1.cds

Sequence Viewer

Length: 1161 bp
atgactgttctccggtgggctgcagtggaatcgaggagaaagaggctcgtcccctgcgtcgtgatctccagcaaagataatgtttttaaaggtgtggagaaggtggcagaggtggtgagcttctccgatgtggttgagttggatcaggccgggttggaggagaagaggaagaacacggtgacagagcctgagagagagagagagatctcccaacgaaaccctagtttcgagcctaagcggtggggtaggcaactatctatatacagcgtcggtgatacgcttgacgagcttcacaggatggacctggtggtttacttgatcgatctcttgatcggtggttggctggttttggtcgaaactgggtcaggtcaaaggattagagcatcgaggagtagagctgtttcattcgatcttcgcagcgagtcttggttgaggttcggggagtccgaggcacctgatcgacttcggtttattgggattgagtttcctgaccggtggggttgttgccggttcggaaggaaggcagcagagccagttgtaggtgatggggtttgggtttgggcctatgggcctgggctcaaatttaggttgttgggccctataatcggtgccggcctaggagatccagaggacctatggtcgatcgacaacttgtttacaaacggctacaatgacttggctacggagattgtgaataaggttatggaagagcttgacatgcccaaggtggttattactactggcattattgaagagtacatcgatgccggcaagaccaaggaggctctccatgtctcagtgctaactcatgcattgccacgggtgtatgaggcgttttctcggcaagagaaaatggaggtcaaccagtttcttggggagatgaaagccaaggggtttgtggctgacaataaggcaatgagggctatgagactggttctcaaggccaaaggagctgtgatcaaaagcatcatcaccaacattctcttctcaaatgagttagaagactactcagacgattcagatgatgatgatgatgaaatacagaactatagcaaagagatggaattgaggttccctccggatagttatttatcttttagcaagaaacatgtcatcgaggatggcccagaaaaccccaaagatttgcaaagtcttcgataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

386

Amino Acids

43.81

Weight (kDa)

5.13

Isoelectric Point (pI)

45.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031300)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004343.1_g000003
rosa_samantha Rh6AG007800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 451, 608
AccIII TCCGGA 1 cut(s) 1078
AciI CCGC 1 cut(s) 238
AclWI GGATC 2 cut(s) 150, 617
AcsI RAATTY 1 cut(s) 581
AfaI GTAC 1 cut(s) 758
AfiI CCNNNNNNNGG 2 cut(s) 539, 605
AflIII ACRYGT 1 cut(s) 1108
AgeI ACCGGT 1 cut(s) 492
AgsI TTSAA 1 cut(s) 752
AhdI GACNNNNNGTC 1 cut(s) 637
AjnI CCWGG 2 cut(s) 303, 571
AjuI GAANNNNNNNTTGG 2 cut(s) 968, 1000
AloI GAACNNNNNNTCC 2 cut(s) 1055, 1087
AluBI AGCT 5 cut(s) 120, 289, 398, 712, 953
AluI AGCT 5 cut(s) 120, 289, 398, 712, 953
Alw26I GTCTC 2 cut(s) 799, 922
AlwI GGATC 2 cut(s) 150, 617
AlwNI CAGNNNCTG 1 cut(s) 188
Aor13HI TCCGGA 1 cut(s) 1078
AoxI GGCC 7 cut(s) 147, 561, 569, 595, 613, 942, 1123
ApaI GGGCCC 1 cut(s) 599
ApeKI GCWGC 3 cut(s) 20, 417, 524
ApoI RAATTY 1 cut(s) 581
AsiGI ACCGGT 1 cut(s) 492
AspA2I CCTAGG 1 cut(s) 616
AspS9I GGNCC 7 cut(s) 301, 561, 569, 595, 596, 631, 1124
AsuC2I CCSGG 1 cut(s) 151
AsuHPI GGTGA 5 cut(s) 127, 190, 284, 554, 964
AvaII GGWCC 2 cut(s) 301, 631
AvrII CCTAGG 1 cut(s) 616
BaeGI GKGCMC 1 cut(s) 599
BanI GGYRCC 2 cut(s) 451, 608
BanII GRGCYC 2 cut(s) 579, 599
BbsI GAAGAC 2 cut(s) 1008, 1145
BbvI GCAGC 3 cut(s) 7, 429, 536
BccI CCATC 4 cut(s) 292, 539, 1054, 1115
BceAI ACGGC 1 cut(s) 679
BcgI CGANNNNNNTGC 3 cut(s) 12, 46, 1136
BciT130I CCWGG 2 cut(s) 305, 573
BclI TGATCA 1 cut(s) 957
BcnI CCSGG 1 cut(s) 151
BcoDI GTCTC 2 cut(s) 799, 922
BfaI CTAG 2 cut(s) 222, 617
BfmI CTRYAG 2 cut(s) 21, 1048
BglII AGATCT 1 cut(s) 204
BisI GCNGC 3 cut(s) 21, 418, 525
BlnI CCTAGG 1 cut(s) 616
BlsI GCNGC 3 cut(s) 22, 419, 526
Bme1390I CCNGG 3 cut(s) 151, 305, 573
Bme18I GGWCC 2 cut(s) 301, 631
BmeRI GACNNNNNGTC 1 cut(s) 637
BmgT120I GGNCC 7 cut(s) 301, 561, 569, 595, 596, 631, 1124
BmiI GGNNCC 4 cut(s) 453, 597, 610, 1073
BmrFI CCNGG 3 cut(s) 151, 305, 573
BmrI ACTGGG 1 cut(s) 369
BmsI GCATC 3 cut(s) 392, 754, 975
BmuI ACTGGG 1 cut(s) 369
BpiI GAAGAC 2 cut(s) 1008, 1145
BplI GAGNNNNNCTC 2 cut(s) 1060, 1092
BpmI CTGGAG 1 cut(s) 52
Bpu10I CCTNAGC 1 cut(s) 234
BpuEI CTTGAG 1 cut(s) 923
BpuMI CCSGG 1 cut(s) 151
Bsa29I ATCGAT 2 cut(s) 321, 762
BsaJI CCNNGG 7 cut(s) 447, 572, 616, 723, 777, 818, 888
BsaWI WCCGGW 3 cut(s) 12, 492, 1078
Bsc4I CCNNNNNNNGG 2 cut(s) 539, 605
Bse118I RCCGGY 4 cut(s) 492, 507, 611, 767
Bse1I ACTGG 5 cut(s) 364, 533, 745, 865, 936
Bse3DI GCAATG 2 cut(s) 812, 921
BseAI TCCGGA 1 cut(s) 1078
BseBI CCWGG 2 cut(s) 305, 573
BseCI ATCGAT 2 cut(s) 321, 762
BseDI CCNNGG 7 cut(s) 447, 572, 616, 723, 777, 818, 888
BseGI GGATG 2 cut(s) 303, 1126
BseLI CCNNNNNNNGG 2 cut(s) 539, 605
BseMI GCAATG 2 cut(s) 812, 921
BseMII CTCAG 3 cut(s) 180, 810, 1023
BseNI ACTGG 5 cut(s) 364, 533, 745, 865, 936
BseRI GAGGAG 3 cut(s) 49, 173, 403
BseSI GKGCMC 1 cut(s) 599
BseXI GCAGC 3 cut(s) 7, 429, 536
Bsh1285I CGRYCG 1 cut(s) 645
BshFI GGCC 7 cut(s) 149, 563, 571, 597, 615, 944, 1125
BshNI GGYRCC 2 cut(s) 451, 608
BshTI ACCGGT 1 cut(s) 492
BshVI ATCGAT 2 cut(s) 321, 762
BsiEI CGRYCG 1 cut(s) 645
BsiSI CCGG 7 cut(s) 13, 150, 493, 508, 612, 768, 1079
BslFI GGGAC 1 cut(s) 35
BslI CCNNNNNNNGG 2 cut(s) 539, 605
BsmAI GTCTC 2 cut(s) 799, 922
BsmFI GGGAC 1 cut(s) 35
BsnI GGCC 7 cut(s) 149, 563, 571, 597, 615, 944, 1125
Bsp120I GGGCCC 1 cut(s) 595
Bsp1286I GDGCHC 2 cut(s) 579, 599
Bsp13I TCCGGA 1 cut(s) 1078
BspACI CCGC 1 cut(s) 238
BspANI GGCC 7 cut(s) 149, 563, 571, 597, 615, 944, 1125
BspCNI CTCAG 3 cut(s) 181, 809, 1022
BspDI ATCGAT 2 cut(s) 321, 762
BspEI TCCGGA 1 cut(s) 1078
BspLI GGNNCC 4 cut(s) 453, 597, 610, 1073
BspMAI CTGCAG 1 cut(s) 25
BspPI GGATC 2 cut(s) 150, 617
BspQI GCTCTTC 1 cut(s) 702
BspT107I GGYRCC 2 cut(s) 451, 608
BsrDI GCAATG 2 cut(s) 812, 921
BsrFI RCCGGY 4 cut(s) 492, 507, 611, 767
BsrI ACTGG 5 cut(s) 364, 533, 745, 865, 936
BssAI RCCGGY 4 cut(s) 492, 507, 611, 767
BssECI CCNNGG 7 cut(s) 447, 572, 616, 723, 777, 818, 888
BssT1I CCWWGG 4 cut(s) 616, 723, 777, 888
Bst2UI CCWGG 2 cut(s) 305, 573
Bst4CI ACNGT 2 cut(s) 7, 178
Bst6I CTCTTC 4 cut(s) 158, 702, 747, 989
BstC8I GCNNGC 2 cut(s) 613, 769
BstDEI CTNAG 4 cut(s) 189, 234, 796, 1009
BstDSI CCRYGG 1 cut(s) 818
BstF5I GGATG 2 cut(s) 303, 1126
BstMAI GTCTC 2 cut(s) 799, 922
BstMCI CGRYCG 1 cut(s) 645
BstMWI GCNNNNNNNGC 4 cut(s) 286, 718, 920, 950
BstNI CCWGG 2 cut(s) 305, 573
BstNSI RCATGY 2 cut(s) 721, 1112
BstSCI CCNGG 3 cut(s) 149, 303, 571
BstSFI CTRYAG 2 cut(s) 21, 1048
BstSLI GKGCMC 1 cut(s) 599
BstV1I GCAGC 3 cut(s) 7, 429, 536
BstV2I GAAGAC 2 cut(s) 1008, 1145
BstX2I RGATCY 2 cut(s) 204, 622
BstXI CCANNNNNNTGG 1 cut(s) 872
BstYI RGATCY 2 cut(s) 204, 622
Bsu15I ATCGAT 2 cut(s) 321, 762
BsuRI GGCC 7 cut(s) 149, 563, 571, 597, 615, 944, 1125
BsuTUI ATCGAT 2 cut(s) 321, 762
BtgI CCRYGG 1 cut(s) 818
BtsCI GGATG 2 cut(s) 303, 1126
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 2 cut(s) 30, 804
Cac8I GCNNGC 2 cut(s) 613, 769
CaiI CAGNNNCTG 1 cut(s) 188
Cfr10I RCCGGY 4 cut(s) 492, 507, 611, 767
Cfr13I GGNCC 7 cut(s) 301, 561, 569, 595, 596, 631, 1124
ClaI ATCGAT 2 cut(s) 321, 762
CseI GACGC 2 cut(s) 46, 256
CsiI ACCWGGT 1 cut(s) 303
Csp6I GTAC 1 cut(s) 757
CspAI ACCGGT 1 cut(s) 492
CviAII CATG 4 cut(s) 718, 791, 809, 1109
CviQI GTAC 1 cut(s) 757
DdeI CTNAG 4 cut(s) 189, 234, 796, 1009
DraI TTTAAA 1 cut(s) 88
DriI GACNNNNNGTC 1 cut(s) 637
Eam1104I CTCTTC 4 cut(s) 158, 702, 747, 989
Eam1105I GACNNNNNGTC 1 cut(s) 637
EarI CTCTTC 4 cut(s) 158, 702, 747, 989
Eco130I CCWWGG 4 cut(s) 616, 723, 777, 888
Eco24I GRGCYC 2 cut(s) 579, 599
Eco47I GGWCC 2 cut(s) 301, 631
EcoO109I RGGNCCY 2 cut(s) 596, 631
EcoRII CCWGG 2 cut(s) 303, 571
EcoT14I CCWWGG 4 cut(s) 616, 723, 777, 888
EcoT22I ATGCAT 1 cut(s) 814
EcoT38I GRGCYC 2 cut(s) 579, 599
ErhI CCWWGG 4 cut(s) 616, 723, 777, 888
FaeI CATG 4 cut(s) 721, 794, 812, 1112
FaqI GGGAC 1 cut(s) 35
FatI CATG 4 cut(s) 717, 790, 808, 1108
FbaI TGATCA 1 cut(s) 957
Fnu4HI GCNGC 3 cut(s) 21, 418, 525
FokI GGATG 2 cut(s) 310, 1133
FriOI GRGCYC 2 cut(s) 579, 599
Fsp4HI GCNGC 3 cut(s) 21, 418, 525
FspBI CTAG 2 cut(s) 222, 617
GluI GCNGC 3 cut(s) 21, 418, 525
GsuI CTGGAG 1 cut(s) 52
HaeIII GGCC 7 cut(s) 149, 563, 571, 597, 615, 944, 1125
HapII CCGG 7 cut(s) 13, 150, 493, 508, 612, 768, 1079
HgaI GACGC 2 cut(s) 46, 256
Hin1II CATG 4 cut(s) 721, 794, 812, 1112
HincII GTYRAC 1 cut(s) 862
HindII GTYRAC 1 cut(s) 862
HinfI GANTC 4 cut(s) 29, 422, 443, 1016
HpaII CCGG 7 cut(s) 13, 150, 493, 508, 612, 768, 1079
HphI GGTGA 5 cut(s) 127, 190, 284, 554, 964
Hpy166II GTNNAC 3 cut(s) 313, 657, 862
Hpy188I TCNGA 5 cut(s) 127, 448, 515, 1012, 1021
Hpy188III TCNNGA 5 cut(s) 61, 328, 488, 626, 1079
Hpy8I GTNNAC 3 cut(s) 313, 657, 862
Hpy99I CGWCG 2 cut(s) 62, 272
HpyAV CCTTC 3 cut(s) 94, 510, 514
HpyCH4III ACNGT 2 cut(s) 7, 178
HpyCH4V TGCA 3 cut(s) 23, 812, 1147
HpyF10VI GCNNNNNNNGC 4 cut(s) 286, 718, 920, 950
HpyF3I CTNAG 4 cut(s) 189, 234, 796, 1009
Hsp92II CATG 4 cut(s) 721, 794, 812, 1112
Kpn2I TCCGGA 1 cut(s) 1078
KroI GCCGGC 2 cut(s) 611, 767
KroNI GCCGGC 2 cut(s) 613, 769
Ksp22I TGATCA 1 cut(s) 957
LguI GCTCTTC 1 cut(s) 702
LmnI GCTCC 1 cut(s) 950
Lsp1109I GCAGC 3 cut(s) 7, 429, 536
LweI GCATC 3 cut(s) 392, 754, 975
MabI ACCWGGT 1 cut(s) 303
MaeI CTAG 2 cut(s) 222, 617
MaeIII GTNAC 1 cut(s) 178
MboII GAAGA 8 cut(s) 175, 181, 404, 719, 764, 976, 1013, 1145
MflI RGATCY 2 cut(s) 204, 622
MhlI GDGCHC 2 cut(s) 579, 599
MluCI AATT 2 cut(s) 581, 1064
MlyI GAGTC 2 cut(s) 431, 452
MmeI TCCRAC 2 cut(s) 120, 135
Mph1103I ATGCAT 1 cut(s) 814
MroI TCCGGA 1 cut(s) 1078
MroNI GCCGGC 2 cut(s) 611, 767
MseI TTAA 1 cut(s) 87
MspI CCGG 7 cut(s) 13, 150, 493, 508, 612, 768, 1079
MspR9I CCNGG 3 cut(s) 151, 305, 573
MvaI CCWGG 2 cut(s) 305, 573
MwoI GCNNNNNNNGC 4 cut(s) 286, 718, 920, 950
NaeI GCCGGC 2 cut(s) 613, 769
NciI CCSGG 1 cut(s) 151
NgoMIV GCCGGC 2 cut(s) 611, 767
NlaIII CATG 4 cut(s) 721, 794, 812, 1112
NlaIV GGNNCC 4 cut(s) 453, 597, 610, 1073
NmeAIII GCCGAG 1 cut(s) 820
NmuCI GTSAC 1 cut(s) 178
NsiI ATGCAT 1 cut(s) 814
NspI RCATGY 2 cut(s) 721, 1112
PciI ACATGT 1 cut(s) 1108
PciSI GCTCTTC 1 cut(s) 702
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 444
PdiI GCCGGC 2 cut(s) 613, 769
PfeI GAWTC 2 cut(s) 29, 1016
PinAI ACCGGT 1 cut(s) 492
PkrI GCNGC 3 cut(s) 22, 419, 526
Ple19I CGATCG 1 cut(s) 645
PleI GAGTC 2 cut(s) 430, 451
PpsI GAGTC 2 cut(s) 430, 451
PpuMI RGGWCCY 1 cut(s) 631
PscI ACATGT 1 cut(s) 1108
Psp5II RGGWCCY 1 cut(s) 631
Psp6I CCWGG 2 cut(s) 303, 571
PspGI CCWGG 2 cut(s) 303, 571
PspN4I GGNNCC 4 cut(s) 453, 597, 610, 1073
PspOMI GGGCCC 1 cut(s) 595
PspPI GGNCC 7 cut(s) 301, 561, 569, 595, 596, 631, 1124
PspPPI RGGWCCY 1 cut(s) 631
PstI CTGCAG 1 cut(s) 25
PstNI CAGNNNCTG 1 cut(s) 188
PsuI RGATCY 2 cut(s) 204, 622
PvuI CGATCG 1 cut(s) 645
RsaI GTAC 1 cut(s) 758
RsaNI GTAC 1 cut(s) 757
SapI GCTCTTC 1 cut(s) 702
SaqAI TTAA 1 cut(s) 87
SatI GCNGC 3 cut(s) 21, 418, 525
Sau96I GGNCC 7 cut(s) 301, 561, 569, 595, 596, 631, 1124
SchI GAGTC 2 cut(s) 431, 452
ScrFI CCNGG 3 cut(s) 151, 305, 573
SduI GDGCHC 2 cut(s) 579, 599
SexAI ACCWGGT 1 cut(s) 303
SfaNI GCATC 3 cut(s) 392, 754, 975
SfcI CTRYAG 2 cut(s) 21, 1048
SinI GGWCC 2 cut(s) 301, 631
SmlI CTYRAG 1 cut(s) 938
SmoI CTYRAG 1 cut(s) 938
Sse9I AATT 2 cut(s) 581, 1064
SsiI CCGC 1 cut(s) 238
SspMI CTAG 2 cut(s) 222, 617
StyD4I CCNGG 3 cut(s) 149, 303, 571
StyI CCWWGG 4 cut(s) 616, 723, 777, 888
TaaI ACNGT 2 cut(s) 7, 178
TasI AATT 2 cut(s) 581, 1064
TatI WGTACW 1 cut(s) 756
TfiI GAWTC 2 cut(s) 29, 1016
Tru1I TTAA 1 cut(s) 87
Tru9I TTAA 1 cut(s) 87
TscAI CASTG 2 cut(s) 30, 804
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 3 cut(s) 20, 417, 524
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 3 cut(s) 393, 896, 1050
TspGWI ACGGA 1 cut(s) 698
TspRI CASTG 2 cut(s) 30, 804
VpaK11BI GGWCC 2 cut(s) 301, 631
XapI RAATTY 1 cut(s) 581
XceI RCATGY 2 cut(s) 721, 1112
XcmI CCANNNNNNNNNTGG 2 cut(s) 633, 895
XmaJI CCTAGG 1 cut(s) 616
XspI CTAG 2 cut(s) 222, 617
Zsp2I ATGCAT 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.