Rmu_sc0004566.1_g000004

PP-loop family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004566.1
OG0021321
Physical Location & Seq
Reverse (-)
37171 .. 38942
1772 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 669 bp
atggtaccttcttgtccatctgtcaacttgggagtgagaatcgaaaaggagaagatgtttgcgttttctggcggatttccatattttcaagcgacgtttggtttcaactcgggtctcaaacttgtttcccttgccaagctctacctcgctatatacttgaatttaagtttggaggagattagcataaattggaattttggagttactgaaccatcgtggttcactgttcacttcttctacccagatccctttgctctcttctctcaaccttcaatttatctcaaatggttgctaattcattgtcttcgtcacaattcgttctcgattgaactctttttaggtttaagctcctctcaattctactttagttccaatagtgttgcaaaaatgggaatcagaagtaatgtttcctgttgtaattgggaggatggtaagccgaaacaaggtcgtgtgcaagaaacagctcgcgacatgaggtacaggatatttcagaatgtttgtatcaagaaccagatagctgtgttgcttacggcacatcatgctgatgaccagtaccaagggtcgacggcggaagttgaggcgagtccgttcagcccgggtcctccgctggtggtggccggaaaacggagatgggtccgggtcaggtcacagtcgggtcgcgcggagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATP_bind_3

Protein Analysis

222

Amino Acids

25.15

Weight (kDa)

9.13

Isoelectric Point (pI)

58.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_3 PF01171 124 - 185 1.9e-12 PP-loop domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021321)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0341031
rosa_multiflora Rmu_sc0004566.1_g000004
rosa_samantha Rh5BG448900 Rh5CG471100 Rh5DG462400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 4
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 563
AccII CGCG 3 cut(s) 468, 660, 662
AciI CCGC 4 cut(s) 72, 569, 605, 662
AclWI GGATC 1 cut(s) 239
AcoI YGGCCR 1 cut(s) 615
AcsI RAATTY 2 cut(s) 160, 193
AfaI GTAC 3 cut(s) 6, 479, 554
AfiI CCNNNNNNNGG 2 cut(s) 443, 624
AgsI TTSAA 5 cut(s) 89, 106, 160, 273, 329
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AluBI AGCT 4 cut(s) 139, 348, 464, 518
AluI AGCT 4 cut(s) 139, 348, 464, 518
Alw26I GTCTC 1 cut(s) 119
AlwI GGATC 1 cut(s) 239
Ama87I CYCGRG 2 cut(s) 109, 595
AoxI GGCC 1 cut(s) 615
ApoI RAATTY 2 cut(s) 160, 193
Asp718I GGTACC 1 cut(s) 4
AspLEI GCGC 1 cut(s) 662
AspS9I GGNCC 2 cut(s) 599, 634
AsuC2I CCSGG 3 cut(s) 596, 597, 638
AvaI CYCGRG 2 cut(s) 109, 595
AvaII GGWCC 2 cut(s) 599, 634
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 296
BccI CCATC 4 cut(s) 25, 220, 422, 624
BceAI ACGGC 2 cut(s) 546, 582
BcnI CCSGG 3 cut(s) 596, 597, 638
BcoDI GTCTC 1 cut(s) 119
Bme1390I CCNGG 3 cut(s) 596, 597, 638
Bme18I GGWCC 2 cut(s) 599, 634
BmeT110I CYCGRG 2 cut(s) 109, 595
BmgT120I GGNCC 2 cut(s) 599, 634
BmiI GGNNCC 3 cut(s) 6, 600, 635
BmrFI CCNGG 3 cut(s) 596, 597, 638
BpiI GAAGAC 1 cut(s) 296
BpuMI CCSGG 3 cut(s) 596, 597, 638
BsaI GGTCTC 1 cut(s) 119
BsaJI CCNNGG 2 cut(s) 556, 595
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 2 cut(s) 443, 624
Bse1I ACTGG 1 cut(s) 550
BseDI CCNNGG 2 cut(s) 556, 595
BseGI GGATG 1 cut(s) 433
BseLI CCNNNNNNNGG 2 cut(s) 443, 624
BseNI ACTGG 1 cut(s) 550
BseRI GAGGAG 2 cut(s) 188, 340
Bsh1236I CGCG 3 cut(s) 468, 660, 662
BshFI GGCC 1 cut(s) 617
BshNI GGYRCC 1 cut(s) 4
BsiHKCI CYCGRG 2 cut(s) 109, 595
BsiSI CCGG 3 cut(s) 596, 618, 637
BslI CCNNNNNNNGG 2 cut(s) 443, 624
BsmAI GTCTC 1 cut(s) 119
BsnI GGCC 1 cut(s) 617
Bso31I GGTCTC 1 cut(s) 119
BsoBI CYCGRG 2 cut(s) 109, 595
Bsp143I GATC 1 cut(s) 244
Bsp68I TCGCGA 1 cut(s) 468
BspACI CCGC 4 cut(s) 72, 569, 605, 662
BspANI GGCC 1 cut(s) 617
BspFNI CGCG 3 cut(s) 468, 660, 662
BspLI GGNNCC 3 cut(s) 6, 600, 635
BspPI GGATC 1 cut(s) 239
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 119
BsrI ACTGG 1 cut(s) 550
BssECI CCNNGG 2 cut(s) 556, 595
BssMI GATC 1 cut(s) 244
BssT1I CCWWGG 1 cut(s) 556
Bst4CI ACNGT 2 cut(s) 226, 651
Bst6I CTCTTC 1 cut(s) 263
BstAPI GCANNNNNTGC 1 cut(s) 539
BstC8I GCNNGC 1 cut(s) 466
BstF5I GGATG 1 cut(s) 433
BstFNI CGCG 3 cut(s) 468, 660, 662
BstHHI GCGC 1 cut(s) 662
BstKTI GATC 1 cut(s) 247
BstMAI GTCTC 1 cut(s) 119
BstMBI GATC 1 cut(s) 244
BstMWI GCNNNNNNNGC 1 cut(s) 539
BstSCI CCNGG 3 cut(s) 594, 595, 636
BstUI CGCG 3 cut(s) 468, 660, 662
BstV2I GAAGAC 1 cut(s) 296
BstX2I RGATCY 1 cut(s) 244
BstYI RGATCY 1 cut(s) 244
BsuRI GGCC 1 cut(s) 617
BtsCI GGATG 1 cut(s) 433
BtsIMutI CAGTG 1 cut(s) 222
BtuMI TCGCGA 1 cut(s) 468
Cac8I GCNNGC 1 cut(s) 466
CfoI GCGC 1 cut(s) 662
Cfr13I GGNCC 2 cut(s) 599, 634
Cfr9I CCCGGG 1 cut(s) 595
Csp6I GTAC 3 cut(s) 5, 478, 553
CviAII CATG 2 cut(s) 472, 539
CviJI RGCY 7 cut(s) 139, 348, 436, 464, 518, 594, 617
CviKI_1 RGCY 7 cut(s) 139, 348, 436, 464, 518, 594, 617
CviQI GTAC 3 cut(s) 5, 478, 553
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 615
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
EciI GGCGGA 2 cut(s) 87, 584
Eco130I CCWWGG 1 cut(s) 556
Eco31I GGTCTC 1 cut(s) 119
Eco47I GGWCC 2 cut(s) 599, 634
Eco88I CYCGRG 2 cut(s) 109, 595
EcoO109I RGGNCCY 1 cut(s) 599
EcoT14I CCWWGG 1 cut(s) 556
ErhI CCWWGG 1 cut(s) 556
FaeI CATG 2 cut(s) 475, 542
FaiI YATR 6 cut(s) 82, 152, 154, 185, 473, 540
FatI CATG 2 cut(s) 471, 538
FblI GTMKAC 1 cut(s) 563
FokI GGATG 1 cut(s) 440
GlaI GCGC 1 cut(s) 661
HaeIII GGCC 1 cut(s) 617
HapII CCGG 3 cut(s) 596, 618, 637
HhaI GCGC 1 cut(s) 662
Hin1II CATG 2 cut(s) 475, 542
Hin6I GCGC 1 cut(s) 660
HinP1I GCGC 1 cut(s) 660
HincII GTYRAC 2 cut(s) 25, 564
HindII GTYRAC 2 cut(s) 25, 564
HinfI GANTC 3 cut(s) 39, 393, 583
HpaII CCGG 3 cut(s) 596, 618, 637
Hpy166II GTNNAC 4 cut(s) 25, 222, 229, 564
Hpy188I TCNGA 2 cut(s) 398, 492
Hpy188III TCNNGA 3 cut(s) 322, 467, 505
Hpy8I GTNNAC 4 cut(s) 25, 222, 229, 564
Hpy99I CGWCG 2 cut(s) 97, 568
HpyAV CCTTC 2 cut(s) 18, 279
HpyCH4III ACNGT 2 cut(s) 226, 651
HpyCH4IV ACGT 1 cut(s) 95
HpyCH4V TGCA 2 cut(s) 383, 454
HpyF10VI GCNNNNNNNGC 1 cut(s) 539
HpySE526I ACGT 1 cut(s) 95
Hsp92II CATG 2 cut(s) 475, 542
HspAI GCGC 1 cut(s) 660
KpnI GGTACC 1 cut(s) 8
Kzo9I GATC 1 cut(s) 244
LmnI GCTCC 1 cut(s) 353
MaeII ACGT 1 cut(s) 95
MaeIII GTNAC 3 cut(s) 202, 308, 645
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MboII GAAGA 4 cut(s) 64, 226, 250, 296
MflI RGATCY 1 cut(s) 244
MluCI AATT 8 cut(s) 160, 187, 193, 273, 294, 313, 356, 418
MlyI GAGTC 1 cut(s) 592
MnlI CCTC 7 cut(s) 155, 166, 361, 418, 468, 571, 612
MseI TTAA 2 cut(s) 164, 344
MslI CAYNNNNRTG 1 cut(s) 543
MspA1I CMGCKG 1 cut(s) 607
MspI CCGG 3 cut(s) 596, 618, 637
MspR9I CCNGG 3 cut(s) 596, 597, 638
MvnI CGCG 3 cut(s) 468, 660, 662
MwoI GCNNNNNNNGC 1 cut(s) 539
NciI CCSGG 3 cut(s) 596, 597, 638
NdeII GATC 1 cut(s) 244
NlaIII CATG 2 cut(s) 475, 542
NlaIV GGNNCC 3 cut(s) 6, 600, 635
NmuCI GTSAC 2 cut(s) 308, 645
NruI TCGCGA 1 cut(s) 468
PfeI GAWTC 2 cut(s) 39, 393
PleI GAGTC 1 cut(s) 591
PpsI GAGTC 1 cut(s) 591
PpuMI RGGWCCY 1 cut(s) 599
Psp5II RGGWCCY 1 cut(s) 599
PspN4I GGNNCC 3 cut(s) 6, 600, 635
PspPI GGNCC 2 cut(s) 599, 634
PspPPI RGGWCCY 1 cut(s) 599
PsuI RGATCY 1 cut(s) 244
RruI TCGCGA 1 cut(s) 468
RsaI GTAC 3 cut(s) 6, 479, 554
RsaNI GTAC 3 cut(s) 5, 478, 553
RseI CAYNNNNRTG 1 cut(s) 543
SalI GTCGAC 1 cut(s) 562
SaqAI TTAA 2 cut(s) 164, 344
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 2 cut(s) 599, 634
SchI GAGTC 1 cut(s) 592
ScrFI CCNGG 3 cut(s) 596, 597, 638
SinI GGWCC 2 cut(s) 599, 634
SmaI CCCGGG 1 cut(s) 597
SmiMI CAYNNNNRTG 1 cut(s) 543
Sse9I AATT 8 cut(s) 160, 187, 193, 273, 294, 313, 356, 418
SsiI CCGC 4 cut(s) 72, 569, 605, 662
StyD4I CCNGG 3 cut(s) 594, 595, 636
StyI CCWWGG 1 cut(s) 556
TaaI ACNGT 2 cut(s) 226, 651
TaiI ACGT 1 cut(s) 98
TaqI TCGA 3 cut(s) 42, 323, 563
TasI AATT 8 cut(s) 160, 187, 193, 273, 294, 313, 356, 418
TfiI GAWTC 2 cut(s) 39, 393
Tru1I TTAA 2 cut(s) 164, 344
Tru9I TTAA 2 cut(s) 164, 344
TscAI CASTG 1 cut(s) 229
TseFI GTSAC 2 cut(s) 308, 645
Tsp45I GTSAC 2 cut(s) 308, 645
TspDTI ATGAA 1 cut(s) 287
TspGWI ACGGA 2 cut(s) 576, 640
TspMI CCCGGG 1 cut(s) 595
TspRI CASTG 1 cut(s) 229
VpaK11BI GGWCC 2 cut(s) 599, 634
XapI RAATTY 2 cut(s) 160, 193
XmaI CCCGGG 1 cut(s) 595
XmiI GTMKAC 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.