Rmu_sc0004797.1_g000006

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004797.1
OG0025438
Physical Location & Seq
Reverse (-)
31201 .. 31804
604 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 516 bp
atggcagctgctaaatccacaaggcgtatatgggtcccggggccagtgatagtgggagcaggtccttccggcttagctgccgcggcttgtctcaaggaaaaaggtattccgagcttaatcctagaaaggaccaactgcatagcttcgttgtggcagcttaagacctacgaccgtctctgtcttcatctcccaaaacagttctgcgagctcatactcatgctattccctgcagatttcccaacctacccaaccaagcatcagtttgtgagttacttaaacgcttatgccgagcgctttgatctaaagccggctttcaacacgacggtggtgagtgccaagtacaacagtcgttgcggctttggggttgtgccggagtttgaaggagtgagggactttggtgcccactgtccataccagctcttacaagagcggcgagcgcttcggggggaagaaggtcctagtggtcggatgtgggaattcaggcatggaggtttgcttggatctctgcgattataa

Protein Analysis

171

Amino Acids

19.09

Weight (kDa)

9.25

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_3 PF13738 16 - 114 2.9e-10 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 17 - 115 7.2e-08 Flavin-binding monooxygenase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0025438)

Species Orthologous Gene IDs
rosa_laevigata RLG00000032760
rosa_multiflora Rmu_sc0004797.1_g000005 Rmu_sc0004797.1_g000006

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 514
Acc36I ACCTGC 1 cut(s) 50
AccB1I GGYRCC 1 cut(s) 398
AccBSI CCGCTC 1 cut(s) 430
AccII CGCG 1 cut(s) 83
AciI CCGC 4 cut(s) 81, 83, 354, 430
AclWI GGATC 1 cut(s) 508
AcsI RAATTY 1 cut(s) 476
AfaI GTAC 1 cut(s) 341
AfeI AGCGCT 2 cut(s) 293, 438
AflII CTTAAG 1 cut(s) 158
AgsI TTSAA 2 cut(s) 316, 380
AleI CACNNNNGTG 1 cut(s) 323
AluBI AGCT 7 cut(s) 8, 77, 114, 143, 157, 208, 418
AluI AGCT 7 cut(s) 8, 77, 114, 143, 157, 208, 418
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 2 cut(s) 95, 179
AlwI GGATC 1 cut(s) 508
Ama87I CYCGRG 1 cut(s) 37
Aor51HI AGCGCT 2 cut(s) 293, 438
AoxI GGCC 1 cut(s) 41
ApeKI GCWGC 4 cut(s) 5, 8, 77, 154
ApoI RAATTY 1 cut(s) 476
AspLEI GCGC 2 cut(s) 294, 439
AspS9I GGNCC 5 cut(s) 34, 41, 62, 129, 455
AsuC2I CCSGG 2 cut(s) 38, 39
AsuHPI GGTGA 1 cut(s) 340
AvaI CYCGRG 1 cut(s) 37
AvaII GGWCC 4 cut(s) 34, 62, 129, 455
BaeGI GKGCMC 1 cut(s) 403
BanI GGYRCC 1 cut(s) 398
BanII GRGCYC 1 cut(s) 210
BbsI GAAGAC 1 cut(s) 173
Bbv12I GWGCWC 1 cut(s) 210
BbvI GCAGC 3 cut(s) 17, 64, 166
BcnI CCSGG 2 cut(s) 38, 39
BcoDI GTCTC 2 cut(s) 95, 179
BfaI CTAG 2 cut(s) 122, 459
BfmI CTRYAG 1 cut(s) 228
BfoI RGCGCY 2 cut(s) 295, 440
BfrI CTTAAG 1 cut(s) 158
BfuAI ACCTGC 1 cut(s) 50
BisI GCNGC 8 cut(s) 6, 9, 78, 81, 84, 155, 355, 431
BlpI GCTNAGC 1 cut(s) 73
BlsI GCNGC 8 cut(s) 7, 10, 79, 82, 85, 156, 356, 432
Bme1390I CCNGG 2 cut(s) 38, 39
Bme18I GGWCC 4 cut(s) 34, 62, 129, 455
BmeT110I CYCGRG 1 cut(s) 37
BmgT120I GGNCC 5 cut(s) 34, 41, 62, 129, 455
BmiI GGNNCC 4 cut(s) 35, 36, 42, 400
BmrFI CCNGG 2 cut(s) 38, 39
BmsI GCATC 1 cut(s) 265
BpiI GAAGAC 1 cut(s) 173
Bpu1102I GCTNAGC 1 cut(s) 73
BpuEI CTTGAG 1 cut(s) 77
BpuMI CCSGG 2 cut(s) 38, 39
BsaJI CCNNGG 3 cut(s) 37, 38, 81
Bse118I RCCGGY 1 cut(s) 307
Bse1I ACTGG 1 cut(s) 44
BseDI CCNNGG 3 cut(s) 37, 38, 81
BseGI GGATG 1 cut(s) 474
BseNI ACTGG 1 cut(s) 44
BseSI GKGCMC 1 cut(s) 403
BseXI GCAGC 3 cut(s) 17, 64, 166
Bsh1236I CGCG 1 cut(s) 83
Bsh1285I CGRYCG 1 cut(s) 172
BshFI GGCC 1 cut(s) 43
BshNI GGYRCC 1 cut(s) 398
BsiEI CGRYCG 1 cut(s) 172
BsiHKAI GWGCWC 1 cut(s) 210
BsiHKCI CYCGRG 1 cut(s) 37
BsiSI CCGG 4 cut(s) 38, 69, 308, 371
BslFI GGGAC 2 cut(s) 20, 404
BsmAI GTCTC 2 cut(s) 95, 179
BsmBI CGTCTC 1 cut(s) 179
BsmFI GGGAC 2 cut(s) 20, 404
BsnI GGCC 1 cut(s) 43
BsoBI CYCGRG 1 cut(s) 37
Bsp1286I GDGCHC 2 cut(s) 210, 403
Bsp143I GATC 2 cut(s) 298, 500
Bsp1720I GCTNAGC 1 cut(s) 73
BspACI CCGC 4 cut(s) 81, 83, 354, 430
BspANI GGCC 1 cut(s) 43
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 4 cut(s) 35, 36, 42, 400
BspMAI CTGCAG 1 cut(s) 232
BspMI ACCTGC 1 cut(s) 50
BspPI GGATC 1 cut(s) 508
BspT107I GGYRCC 1 cut(s) 398
BspTI CTTAAG 1 cut(s) 158
BsrBI CCGCTC 1 cut(s) 430
BsrFI RCCGGY 1 cut(s) 307
BsrI ACTGG 1 cut(s) 44
BssAI RCCGGY 1 cut(s) 307
BssECI CCNNGG 3 cut(s) 37, 38, 81
BssMI GATC 2 cut(s) 298, 500
Bst4CI ACNGT 5 cut(s) 173, 198, 325, 347, 407
BstAFI CTTAAG 1 cut(s) 158
BstC8I GCNNGC 3 cut(s) 206, 309, 435
BstDEI CTNAG 1 cut(s) 73
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 1 cut(s) 474
BstFNI CGCG 1 cut(s) 83
BstH2I RGCGCY 2 cut(s) 295, 440
BstHHI GCGC 2 cut(s) 294, 439
BstKTI GATC 2 cut(s) 301, 503
BstMAI GTCTC 2 cut(s) 95, 179
BstMBI GATC 2 cut(s) 298, 500
BstMCI CGRYCG 1 cut(s) 172
BstMWI GCNNNNNNNGC 2 cut(s) 83, 436
BstSCI CCNGG 2 cut(s) 36, 37
BstSFI CTRYAG 1 cut(s) 228
BstSLI GKGCMC 1 cut(s) 403
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 3 cut(s) 17, 64, 166
BstV2I GAAGAC 1 cut(s) 173
BstX2I RGATCY 1 cut(s) 500
BstYI RGATCY 1 cut(s) 500
BsuRI GGCC 1 cut(s) 43
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 1 cut(s) 474
BtsIMutI CAGTG 2 cut(s) 51, 403
BveI ACCTGC 1 cut(s) 50
Cac8I GCNNGC 3 cut(s) 206, 309, 435
CfoI GCGC 2 cut(s) 294, 439
Cfr10I RCCGGY 1 cut(s) 307
Cfr13I GGNCC 5 cut(s) 34, 41, 62, 129, 455
Cfr42I CCGCGG 1 cut(s) 84
Cfr9I CCCGGG 1 cut(s) 37
Csp6I GTAC 1 cut(s) 340
CviAII CATG 2 cut(s) 217, 485
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 1 cut(s) 73
DpnI GATC 2 cut(s) 300, 502
DpnII GATC 2 cut(s) 298, 500
Ecl136II GAGCTC 1 cut(s) 208
Eco24I GRGCYC 1 cut(s) 210
Eco47I GGWCC 4 cut(s) 34, 62, 129, 455
Eco47III AGCGCT 2 cut(s) 293, 438
Eco53kI GAGCTC 1 cut(s) 208
Eco88I CYCGRG 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 208
EcoO109I RGGNCCY 3 cut(s) 34, 62, 455
EcoRI GAATTC 1 cut(s) 476
EcoT38I GRGCYC 1 cut(s) 210
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 2 cut(s) 220, 488
FaiI YATR 9 cut(s) 29, 31, 140, 212, 218, 285, 412, 486, 514
FaqI GGGAC 2 cut(s) 20, 404
FatI CATG 2 cut(s) 216, 484
Fnu4HI GCNGC 8 cut(s) 6, 9, 78, 81, 84, 155, 355, 431
FokI GGATG 1 cut(s) 481
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 8 cut(s) 6, 9, 78, 81, 84, 155, 355, 431
FspBI CTAG 2 cut(s) 122, 459
GlaI GCGC 2 cut(s) 293, 438
GluI GCNGC 8 cut(s) 6, 9, 78, 81, 84, 155, 355, 431
HaeII RGCGCY 2 cut(s) 295, 440
HaeIII GGCC 1 cut(s) 43
HapII CCGG 4 cut(s) 38, 69, 308, 371
HhaI GCGC 2 cut(s) 294, 439
Hin1II CATG 2 cut(s) 220, 488
Hin6I GCGC 2 cut(s) 292, 437
HinP1I GCGC 2 cut(s) 292, 437
HpaII CCGG 4 cut(s) 38, 69, 308, 371
HphI GGTGA 1 cut(s) 340
Hpy188I TCNGA 2 cut(s) 111, 468
Hpy99I CGWCG 1 cut(s) 325
HpyAV CCTTC 3 cut(s) 75, 374, 446
HpyCH4III ACNGT 5 cut(s) 173, 198, 325, 347, 407
HpyCH4V TGCA 2 cut(s) 138, 230
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 436
HpyF3I CTNAG 1 cut(s) 73
Hsp92II CATG 2 cut(s) 220, 488
HspAI GCGC 2 cut(s) 292, 437
KflI GGGWCCC 1 cut(s) 34
KroI GCCGGC 1 cut(s) 307
KroNI GCCGGC 1 cut(s) 309
KspI CCGCGG 1 cut(s) 84
Kzo9I GATC 2 cut(s) 298, 500
LmnI GCTCC 1 cut(s) 56
LpnPI CCDG 9 cut(s) 45, 51, 57, 82, 240, 321, 384, 428, 466
Lsp1109I GCAGC 3 cut(s) 17, 64, 166
LweI GCATC 1 cut(s) 265
MaeI CTAG 2 cut(s) 122, 459
MaeIII GTNAC 1 cut(s) 269
MalI GATC 2 cut(s) 300, 502
MbiI CCGCTC 1 cut(s) 430
MboI GATC 2 cut(s) 298, 500
MboII GAAGA 2 cut(s) 173, 461
MflI RGATCY 1 cut(s) 500
MhlI GDGCHC 2 cut(s) 210, 403
MluCI AATT 1 cut(s) 476
MmeI TCCRAC 1 cut(s) 446
MnlI CCTC 2 cut(s) 381, 482
MroNI GCCGGC 1 cut(s) 307
MseI TTAA 3 cut(s) 116, 159, 275
MslI CAYNNNNRTG 2 cut(s) 215, 323
MspA1I CMGCKG 2 cut(s) 8, 83
MspCI CTTAAG 1 cut(s) 158
MspI CCGG 4 cut(s) 38, 69, 308, 371
MspR9I CCNGG 2 cut(s) 38, 39
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 2 cut(s) 83, 436
NaeI GCCGGC 1 cut(s) 309
NciI CCSGG 2 cut(s) 38, 39
NdeII GATC 2 cut(s) 298, 500
NgoMIV GCCGGC 1 cut(s) 307
NlaIII CATG 2 cut(s) 220, 488
NlaIV GGNNCC 4 cut(s) 35, 36, 42, 400
NmeAIII GCCGAG 1 cut(s) 313
OliI CACNNNNGTG 1 cut(s) 323
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PdiI GCCGGC 1 cut(s) 309
PkrI GCNGC 8 cut(s) 7, 10, 79, 82, 85, 156, 356, 432
PpuMI RGGWCCY 3 cut(s) 34, 62, 455
PsiI TTATAA 1 cut(s) 514
Psp124BI GAGCTC 1 cut(s) 210
Psp5II RGGWCCY 3 cut(s) 34, 62, 455
PspN4I GGNNCC 4 cut(s) 35, 36, 42, 400
PspPI GGNCC 5 cut(s) 34, 41, 62, 129, 455
PspPPI RGGWCCY 3 cut(s) 34, 62, 455
PstI CTGCAG 1 cut(s) 232
PsuI RGATCY 1 cut(s) 500
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
RseI CAYNNNNRTG 2 cut(s) 215, 323
SacI GAGCTC 1 cut(s) 210
SacII CCGCGG 1 cut(s) 84
SaqAI TTAA 3 cut(s) 116, 159, 275
SatI GCNGC 8 cut(s) 6, 9, 78, 81, 84, 155, 355, 431
Sau3AI GATC 2 cut(s) 298, 500
Sau96I GGNCC 5 cut(s) 34, 41, 62, 129, 455
ScrFI CCNGG 2 cut(s) 38, 39
SduI GDGCHC 2 cut(s) 210, 403
SfaNI GCATC 1 cut(s) 265
SfcI CTRYAG 1 cut(s) 228
Sfr303I CCGCGG 1 cut(s) 84
SgrBI CCGCGG 1 cut(s) 84
SinI GGWCC 4 cut(s) 34, 62, 129, 455
SmaI CCCGGG 1 cut(s) 39
SmiMI CAYNNNNRTG 2 cut(s) 215, 323
SmlI CTYRAG 2 cut(s) 92, 158
SmoI CTYRAG 2 cut(s) 92, 158
Sse9I AATT 1 cut(s) 476
SsiI CCGC 4 cut(s) 81, 83, 354, 430
SspMI CTAG 2 cut(s) 122, 459
SstI GAGCTC 1 cut(s) 210
StyD4I CCNGG 2 cut(s) 36, 37
TaaI ACNGT 5 cut(s) 173, 198, 325, 347, 407
TasI AATT 1 cut(s) 476
TatI WGTACW 1 cut(s) 339
TauI GCSGC 4 cut(s) 83, 86, 357, 433
Tru1I TTAA 3 cut(s) 116, 159, 275
Tru9I TTAA 3 cut(s) 116, 159, 275
TscAI CASTG 2 cut(s) 51, 410
TseI GCWGC 4 cut(s) 5, 8, 77, 154
TspDTI ATGAA 1 cut(s) 173
TspMI CCCGGG 1 cut(s) 37
TspRI CASTG 2 cut(s) 51, 410
Vha464I CTTAAG 1 cut(s) 158
VpaK11BI GGWCC 4 cut(s) 34, 62, 129, 455
XapI RAATTY 1 cut(s) 476
XmaI CCCGGG 1 cut(s) 37
XspI CTAG 2 cut(s) 122, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.