Rmu_sc0004814.1_g000001

Protein of unknown function (DUF3522)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004814.1
OG0024381
Physical Location & Seq
Reverse (-)
41 .. 2158
2118 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 816 bp
atggctcttcatctaattctctgtatgaaatttgacatagggcacatatggcaatcaatttttcttatcgcgtcaaatgctgcagcagcacttcctgccttctattcccttcgacagaaggcatggtcagaatgggtgatatatacatctagtggaattgcaagtggaatttatcatgcctgtgatgtgggaacatggtgtcctctggattttcatactttacagttcatggacttctggctgtctttcatggccgtagtgagcacatttgtgtacctagctacaatggatgaagctcataagagagcagtcctcacagctgtagcaatttttactgcccttatggcagttactaaggccacaaggacgtccaatattgcgattgtaatgtcaattggcatttcggctcttcttcttggatggctgatagaattaacaaccaagtataggtcattttcctttccggttggattctccttaaatataattgagaggttccaaggtgttagaggatggctcaaagatcttattaagacagtttccaaaagatttcgctggggctttgtacttgcaggttctactgcattagccatggcagcaatcagccggacactggagagcactgaaacctactggatttggcacagcctttggcatgtcaccatatatacgtcttccttcttcttcctttgctcaaaagcaagtactgagagtactgaaaatactgtagatactagtattgtagatactgagagtcagagacccccaaatggagcctatgagttgactaggcaggattcatttccaagaagttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3522

Protein Analysis

271

Amino Acids

30.5

Weight (kDa)

8.34

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3522 PF12036 15 - 220 3.5e-40 Protein of unknown function (DUF3522)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024381)

Species Orthologous Gene IDs
malus_domestica MD01G1067000.v1.1
pyrus_communis pycom07g13140
rosa_multiflora Rmu_sc0004814.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 371
Acc36I ACCTGC 1 cut(s) 563
AccII CGCG 1 cut(s) 71
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 2 cut(s) 29, 168
AcyI GRCGYC 1 cut(s) 368
AfaI GTAC 4 cut(s) 275, 567, 706, 715
AfiI CCNNNNNNNGG 3 cut(s) 447, 613, 770
AhlI ACTAGT 1 cut(s) 734
AleI CACNNNNGTG 1 cut(s) 269
AloI GAACNNNNNNTCC 2 cut(s) 184, 216
AluBI AGCT 3 cut(s) 281, 296, 320
AluI AGCT 3 cut(s) 281, 296, 320
Alw21I GWGCWC 2 cut(s) 266, 623
Alw26I GTCTC 1 cut(s) 754
AoxI GGCC 2 cut(s) 252, 357
ApeKI GCWGC 4 cut(s) 80, 83, 86, 596
ApoI RAATTY 2 cut(s) 29, 168
AsuHPI GGTGA 2 cut(s) 148, 652
BaeGI GKGCMC 1 cut(s) 45
BbsI GAAGAC 1 cut(s) 666
Bbv12I GWGCWC 2 cut(s) 266, 623
BbvI GCAGC 4 cut(s) 67, 95, 98, 608
BccI CCATC 2 cut(s) 414, 507
BceAI ACGGC 1 cut(s) 239
BcoDI GTCTC 1 cut(s) 754
BcuI ACTAGT 1 cut(s) 734
BfaI CTAG 4 cut(s) 150, 278, 735, 789
BfmI CTRYAG 3 cut(s) 81, 321, 726
BfuAI ACCTGC 1 cut(s) 563
BglI GCCNNNNNGGC 1 cut(s) 344
BglII AGATCT 1 cut(s) 523
BisI GCNGC 4 cut(s) 81, 84, 87, 597
BlsI GCNGC 4 cut(s) 82, 85, 88, 598
BmcAI AGTACT 2 cut(s) 706, 715
BmiI GGNNCC 2 cut(s) 497, 775
BpiI GAAGAC 1 cut(s) 666
BplI GAGNNNNNCTC 4 cut(s) 297, 329, 501, 533
BpmI CTGGAG 1 cut(s) 635
BsaHI GRCGYC 1 cut(s) 368
BsaI GGTCTC 1 cut(s) 754
BsaJI CCNNGG 2 cut(s) 499, 591
BsaWI WCCGGW 1 cut(s) 463
Bsc4I CCNNNNNNNGG 3 cut(s) 447, 613, 770
Bse1I ACTGG 2 cut(s) 618, 638
BseDI CCNNGG 2 cut(s) 499, 591
BseGI GGATG 3 cut(s) 295, 425, 518
BseLI CCNNNNNNNGG 3 cut(s) 447, 613, 770
BseMII CTCAG 2 cut(s) 699, 741
BseNI ACTGG 2 cut(s) 618, 638
BseSI GKGCMC 1 cut(s) 45
BseXI GCAGC 4 cut(s) 67, 95, 98, 608
BseYI CCCAGC 1 cut(s) 555
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 2 cut(s) 254, 359
BsiHKAI GWGCWC 2 cut(s) 266, 623
BsiSI CCGG 2 cut(s) 464, 607
BslI CCNNNNNNNGG 3 cut(s) 447, 613, 770
BsmAI GTCTC 1 cut(s) 754
BsnI GGCC 2 cut(s) 254, 359
Bso31I GGTCTC 1 cut(s) 754
Bsp1286I GDGCHC 3 cut(s) 45, 266, 623
Bsp143I GATC 1 cut(s) 523
Bsp19I CCATGG 1 cut(s) 591
BspANI GGCC 2 cut(s) 254, 359
BspCNI CTCAG 2 cut(s) 700, 742
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 2 cut(s) 497, 775
BspMAI CTGCAG 1 cut(s) 85
BspMI ACCTGC 1 cut(s) 563
BspQI GCTCTTC 2 cut(s) 12, 414
BspTNI GGTCTC 1 cut(s) 754
BsrI ACTGG 2 cut(s) 618, 638
BssECI CCNNGG 2 cut(s) 499, 591
BssMI GATC 1 cut(s) 523
BssNI GRCGYC 1 cut(s) 368
BssT1I CCWWGG 2 cut(s) 499, 591
Bst4CI ACNGT 3 cut(s) 225, 538, 727
Bst6I CTCTTC 2 cut(s) 12, 414
BstACI GRCGYC 1 cut(s) 368
BstAPI GCANNNNNTGC 1 cut(s) 95
BstDEI CTNAG 3 cut(s) 354, 708, 750
BstDSI CCRYGG 1 cut(s) 591
BstF5I GGATG 3 cut(s) 295, 425, 518
BstFNI CGCG 1 cut(s) 71
BstKTI GATC 1 cut(s) 526
BstMAI GTCTC 1 cut(s) 754
BstMBI GATC 1 cut(s) 523
BstMWI GCNNNNNNNGC 6 cut(s) 49, 77, 86, 95, 344, 596
BstNSI RCATGY 1 cut(s) 659
BstSFI CTRYAG 3 cut(s) 81, 321, 726
BstSLI GKGCMC 1 cut(s) 45
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 4 cut(s) 67, 95, 98, 608
BstV2I GAAGAC 1 cut(s) 666
BstX2I RGATCY 1 cut(s) 523
BstYI RGATCY 1 cut(s) 523
BsuRI GGCC 2 cut(s) 254, 359
BtgI CCRYGG 1 cut(s) 591
BtsCI GGATG 3 cut(s) 295, 425, 518
BtsIMutI CAGTG 2 cut(s) 611, 621
BveI ACCTGC 1 cut(s) 563
CseI GACGC 1 cut(s) 60
Csp6I GTAC 4 cut(s) 274, 566, 705, 714
CviAII CATG 7 cut(s) 123, 176, 195, 229, 250, 592, 656
CviQI GTAC 4 cut(s) 274, 566, 705, 714
DdeI CTNAG 3 cut(s) 354, 708, 750
DpnI GATC 1 cut(s) 525
DpnII GATC 1 cut(s) 523
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 2 cut(s) 12, 414
EarI CTCTTC 2 cut(s) 12, 414
Eco130I CCWWGG 2 cut(s) 499, 591
Eco31I GGTCTC 1 cut(s) 754
EcoT14I CCWWGG 2 cut(s) 499, 591
ErhI CCWWGG 2 cut(s) 499, 591
FaeI CATG 7 cut(s) 126, 179, 198, 232, 253, 595, 659
FatI CATG 7 cut(s) 122, 175, 194, 228, 249, 591, 655
FauNDI CATATG 1 cut(s) 47
Fnu4HI GCNGC 4 cut(s) 81, 84, 87, 597
FokI GGATG 3 cut(s) 302, 432, 525
Fsp4HI GCNGC 4 cut(s) 81, 84, 87, 597
FspBI CTAG 4 cut(s) 150, 278, 735, 789
GluI GCNGC 4 cut(s) 81, 84, 87, 597
GsaI CCCAGC 1 cut(s) 559
GsuI CTGGAG 1 cut(s) 635
HaeIII GGCC 2 cut(s) 254, 359
HapII CCGG 2 cut(s) 464, 607
HgaI GACGC 1 cut(s) 60
Hin1I GRCGYC 1 cut(s) 368
Hin1II CATG 7 cut(s) 126, 179, 198, 232, 253, 595, 659
HincII GTYRAC 1 cut(s) 786
HindII GTYRAC 1 cut(s) 786
HinfI GANTC 3 cut(s) 471, 754, 797
HpaII CCGG 2 cut(s) 464, 607
HphI GGTGA 2 cut(s) 148, 652
Hpy166II GTNNAC 2 cut(s) 274, 786
Hpy188I TCNGA 2 cut(s) 130, 759
Hpy188III TCNNGA 1 cut(s) 206
Hpy8I GTNNAC 2 cut(s) 274, 786
HpyAV CCTTC 4 cut(s) 109, 112, 119, 688
HpyCH4III ACNGT 3 cut(s) 225, 538, 727
HpyCH4IV ACGT 2 cut(s) 368, 671
HpyCH4V TGCA 4 cut(s) 83, 161, 572, 584
HpyF10VI GCNNNNNNNGC 6 cut(s) 49, 77, 86, 95, 344, 596
HpyF3I CTNAG 3 cut(s) 354, 708, 750
HpySE526I ACGT 2 cut(s) 368, 671
Hsp92I GRCGYC 1 cut(s) 368
Hsp92II CATG 7 cut(s) 126, 179, 198, 232, 253, 595, 659
Kzo9I GATC 1 cut(s) 523
LguI GCTCTTC 2 cut(s) 12, 414
LmnI GCTCC 1 cut(s) 773
Lsp1109I GCAGC 4 cut(s) 67, 95, 98, 608
MaeI CTAG 4 cut(s) 150, 278, 735, 789
MaeII ACGT 2 cut(s) 368, 671
MaeIII GTNAC 2 cut(s) 349, 658
MalI GATC 1 cut(s) 525
MboI GATC 1 cut(s) 523
MboII GAAGA 5 cut(s) 401, 404, 666, 673, 676
MfeI CAATTG 1 cut(s) 393
MflI RGATCY 1 cut(s) 523
MhlI GDGCHC 3 cut(s) 45, 266, 623
MluCI AATT 9 cut(s) 15, 29, 57, 156, 168, 327, 393, 431, 486
MlyI GAGTC 1 cut(s) 763
MmeI TCCRAC 1 cut(s) 448
MnlI CCTC 4 cut(s) 213, 323, 486, 503
MseI TTAA 3 cut(s) 434, 479, 531
MslI CAYNNNNRTG 2 cut(s) 180, 269
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 2 cut(s) 464, 607
MunI CAATTG 1 cut(s) 393
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 6 cut(s) 49, 77, 86, 95, 344, 596
NcoI CCATGG 1 cut(s) 591
NdeI CATATG 1 cut(s) 47
NdeII GATC 1 cut(s) 523
NlaIII CATG 7 cut(s) 126, 179, 198, 232, 253, 595, 659
NlaIV GGNNCC 2 cut(s) 497, 775
NmuCI GTSAC 1 cut(s) 658
NspI RCATGY 1 cut(s) 659
OliI CACNNNNGTG 1 cut(s) 269
PciSI GCTCTTC 2 cut(s) 12, 414
PfeI GAWTC 2 cut(s) 471, 797
PkrI GCNGC 4 cut(s) 82, 85, 88, 598
PleI GAGTC 1 cut(s) 762
PpsI GAGTC 1 cut(s) 762
PspFI CCCAGC 1 cut(s) 555
PspN4I GGNNCC 2 cut(s) 497, 775
PstI CTGCAG 1 cut(s) 85
PsuI RGATCY 1 cut(s) 523
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 4 cut(s) 275, 567, 706, 715
RsaNI GTAC 4 cut(s) 274, 566, 705, 714
RseI CAYNNNNRTG 2 cut(s) 180, 269
SapI GCTCTTC 2 cut(s) 12, 414
SaqAI TTAA 3 cut(s) 434, 479, 531
SatI GCNGC 4 cut(s) 81, 84, 87, 597
Sau3AI GATC 1 cut(s) 523
ScaI AGTACT 2 cut(s) 706, 715
SchI GAGTC 1 cut(s) 763
SduI GDGCHC 3 cut(s) 45, 266, 623
SfcI CTRYAG 3 cut(s) 81, 321, 726
SmiMI CAYNNNNRTG 2 cut(s) 180, 269
SpeI ACTAGT 1 cut(s) 734
Sse9I AATT 9 cut(s) 15, 29, 57, 156, 168, 327, 393, 431, 486
SspI AATATT 1 cut(s) 376
SspMI CTAG 4 cut(s) 150, 278, 735, 789
StyI CCWWGG 2 cut(s) 499, 591
TaaI ACNGT 3 cut(s) 225, 538, 727
TaiI ACGT 2 cut(s) 371, 674
TaqI TCGA 1 cut(s) 112
TasI AATT 9 cut(s) 15, 29, 57, 156, 168, 327, 393, 431, 486
TatI WGTACW 3 cut(s) 565, 704, 713
TfiI GAWTC 2 cut(s) 471, 797
Tru1I TTAA 3 cut(s) 434, 479, 531
Tru9I TTAA 3 cut(s) 434, 479, 531
TscAI CASTG 2 cut(s) 618, 628
TseFI GTSAC 1 cut(s) 658
TseI GCWGC 4 cut(s) 80, 83, 86, 596
Tsp45I GTSAC 1 cut(s) 658
TspDTI ATGAA 6 cut(s) 41, 203, 217, 238, 306, 789
TspRI CASTG 2 cut(s) 618, 628
XapI RAATTY 2 cut(s) 29, 168
XceI RCATGY 1 cut(s) 659
XspI CTAG 4 cut(s) 150, 278, 735, 789
ZraI GACGTC 1 cut(s) 369
ZrmI AGTACT 2 cut(s) 706, 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.