Rmu_sc0004836.1_g000002

Chloroplastic group IIA intron splicing facilitator CRS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004836.1
OG0025478
Physical Location & Seq
Reverse (-)
14809 .. 15542
734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004836.1_g000002.1.cds

Sequence Viewer

Length: 534 bp
atgcgagtttctttctgtccgagtgaaattcgtacagaatttcgattcaactcagatggtctctcttctctgttctcggaaagctcgcgtgttgggccagttctcaactcgaaagctcgcgtcttggttgggttttcgaaacagaaagaggaagaagttgatagaaatgggaaattggagagagcaattgatggggtttcacaagaggaaaagataataatttcgaaaagcttcgaacatagaaatggcaaattagagggacagattgatggaatttcagttggtgtttcaaaaaagcaggggatggtgatttcaaaaggcttaattggggctgctgtggatgaaattctatctgtggatagtgaaaatgatgaaaatgtggaaccttttgtgtcttctggtcgtgattctcaagcttctactcagttgccttgggagagagaaggtgaattgggtaatggggaaggtggtaagacaataaagaagtggagtaatacgctttcggctgaggcatccttccggagcatgagttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

177

Amino Acids

19.32

Weight (kDa)

5.26

Isoelectric Point (pI)

35.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025478)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000331.1_g000006 Rmu_sc0004836.1_g000002 Rmu_sc0017886.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 88, 120
AccIII TCCGGA 1 cut(s) 519
AcsI RAATTY 4 cut(s) 27, 38, 273, 345
AfaI GTAC 1 cut(s) 34
AgsI TTSAA 3 cut(s) 49, 291, 315
AluBI AGCT 4 cut(s) 84, 116, 231, 416
AluI AGCT 4 cut(s) 84, 116, 231, 416
Alw26I GTCTC 1 cut(s) 65
Aor13HI TCCGGA 1 cut(s) 519
AoxI GGCC 1 cut(s) 95
ApeKI GCWGC 1 cut(s) 332
ApoI RAATTY 4 cut(s) 27, 38, 273, 345
Asp700I GAANNNNTTC 1 cut(s) 230
AspS9I GGNCC 1 cut(s) 95
AsuHPI GGTGA 2 cut(s) 319, 458
AsuII TTCGAA 3 cut(s) 137, 224, 234
BbsI GAAGAC 1 cut(s) 387
BbvCI CCTCAGC 1 cut(s) 507
BbvI GCAGC 1 cut(s) 319
BccI CCATC 4 cut(s) 50, 185, 263, 298
BcoDI GTCTC 1 cut(s) 65
BisI GCNGC 1 cut(s) 333
BlsI GCNGC 1 cut(s) 334
BmgT120I GGNCC 1 cut(s) 95
BmiI GGNNCC 1 cut(s) 384
BmsI GCATC 1 cut(s) 521
BpiI GAAGAC 1 cut(s) 387
Bpu10I CCTNAGC 1 cut(s) 507
Bpu14I TTCGAA 3 cut(s) 137, 224, 234
BpuEI CTTGAG 1 cut(s) 396
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 1 cut(s) 431
BsaWI WCCGGW 1 cut(s) 519
BsaXI ACNNNNNCTCC 1 cut(s) 514
Bse1I ACTGG 1 cut(s) 98
BseAI TCCGGA 1 cut(s) 519
BseDI CCNNGG 1 cut(s) 431
BseGI GGATG 3 cut(s) 309, 346, 512
BseMII CTCAG 3 cut(s) 66, 437, 498
BseNI ACTGG 1 cut(s) 98
BseXI GCAGC 1 cut(s) 319
Bsh1236I CGCG 2 cut(s) 88, 120
BshFI GGCC 1 cut(s) 97
BsiSI CCGG 1 cut(s) 520
BslFI GGGAC 1 cut(s) 273
BsmAI GTCTC 1 cut(s) 65
BsmFI GGGAC 1 cut(s) 273
BsnI GGCC 1 cut(s) 97
Bso31I GGTCTC 1 cut(s) 65
Bsp119I TTCGAA 3 cut(s) 137, 224, 234
Bsp13I TCCGGA 1 cut(s) 519
BspANI GGCC 1 cut(s) 97
BspCNI CTCAG 3 cut(s) 65, 436, 499
BspEI TCCGGA 1 cut(s) 519
BspFNI CGCG 2 cut(s) 88, 120
BspLI GGNNCC 1 cut(s) 384
BspT104I TTCGAA 3 cut(s) 137, 224, 234
BspTNI GGTCTC 1 cut(s) 65
BsrI ACTGG 1 cut(s) 98
BssECI CCNNGG 1 cut(s) 431
BssT1I CCWWGG 1 cut(s) 431
Bst6I CTCTTC 1 cut(s) 70
BstBI TTCGAA 3 cut(s) 137, 224, 234
BstC8I GCNNGC 2 cut(s) 86, 118
BstDEI CTNAG 3 cut(s) 52, 423, 507
BstF5I GGATG 3 cut(s) 309, 346, 512
BstFNI CGCG 2 cut(s) 88, 120
BstMAI GTCTC 1 cut(s) 65
BstMWI GCNNNNNNNGC 1 cut(s) 94
BstUI CGCG 2 cut(s) 88, 120
BstV1I GCAGC 1 cut(s) 319
BstV2I GAAGAC 1 cut(s) 387
BsuRI GGCC 1 cut(s) 97
BtsCI GGATG 3 cut(s) 309, 346, 512
Cac8I GCNNGC 2 cut(s) 86, 118
Cfr13I GGNCC 1 cut(s) 95
CseI GACGC 1 cut(s) 109
Csp6I GTAC 1 cut(s) 33
CviAII CATG 1 cut(s) 526
CviJI RGCY 8 cut(s) 84, 97, 116, 231, 321, 332, 416, 506
CviKI_1 RGCY 8 cut(s) 84, 97, 116, 231, 321, 332, 416, 506
CviQI GTAC 1 cut(s) 33
DdeI CTNAG 3 cut(s) 52, 423, 507
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 431
Eco31I GGTCTC 1 cut(s) 65
EcoT14I CCWWGG 1 cut(s) 431
ErhI CCWWGG 1 cut(s) 431
FaeI CATG 1 cut(s) 529
FaiI YATR 2 cut(s) 240, 527
FaqI GGGAC 1 cut(s) 273
FatI CATG 1 cut(s) 525
Fnu4HI GCNGC 1 cut(s) 333
FokI GGATG 3 cut(s) 316, 353, 499
Fsp4HI GCNGC 1 cut(s) 333
GluI GCNGC 1 cut(s) 333
HaeIII GGCC 1 cut(s) 97
HapII CCGG 1 cut(s) 520
HgaI GACGC 1 cut(s) 109
Hin1II CATG 1 cut(s) 529
HindIII AAGCTT 2 cut(s) 229, 414
HinfI GANTC 2 cut(s) 45, 407
HpaII CCGG 1 cut(s) 520
HphI GGTGA 2 cut(s) 319, 458
Hpy188I TCNGA 3 cut(s) 21, 55, 79
Hpy188III TCNNGA 2 cut(s) 404, 520
HpyAV CCTTC 3 cut(s) 437, 458, 526
HpyF10VI GCNNNNNNNGC 1 cut(s) 94
HpyF3I CTNAG 3 cut(s) 52, 423, 507
Hsp92II CATG 1 cut(s) 529
Kpn2I TCCGGA 1 cut(s) 519
LmnI GCTCC 1 cut(s) 522
LpnPI CCDG 3 cut(s) 111, 284, 384
Lsp1109I GCAGC 1 cut(s) 319
LweI GCATC 1 cut(s) 521
MboII GAAGA 3 cut(s) 57, 164, 387
MfeI CAATTG 1 cut(s) 186
MnlI CCTC 4 cut(s) 142, 199, 250, 502
MroI TCCGGA 1 cut(s) 519
MroXI GAANNNNTTC 1 cut(s) 230
MseI TTAA 1 cut(s) 323
MslI CAYNNNNRTG 1 cut(s) 243
MspI CCGG 1 cut(s) 520
MunI CAATTG 1 cut(s) 186
MvnI CGCG 2 cut(s) 88, 120
MwoI GCNNNNNNNGC 1 cut(s) 94
NlaIII CATG 1 cut(s) 529
NlaIV GGNNCC 1 cut(s) 384
NspV TTCGAA 3 cut(s) 137, 224, 234
PdmI GAANNNNTTC 1 cut(s) 230
PfeI GAWTC 2 cut(s) 45, 407
PkrI GCNGC 1 cut(s) 334
PspN4I GGNNCC 1 cut(s) 384
PspPI GGNCC 1 cut(s) 95
RsaI GTAC 1 cut(s) 34
RsaNI GTAC 1 cut(s) 33
RseI CAYNNNNRTG 1 cut(s) 243
SaqAI TTAA 1 cut(s) 323
SatI GCNGC 1 cut(s) 333
Sau96I GGNCC 1 cut(s) 95
SetI ASST 7 cut(s) 86, 118, 233, 388, 418, 448, 469
SfaNI GCATC 1 cut(s) 521
SfuI TTCGAA 3 cut(s) 137, 224, 234
SmiMI CAYNNNNRTG 1 cut(s) 243
SmlI CTYRAG 1 cut(s) 411
SmoI CTYRAG 1 cut(s) 411
StyI CCWWGG 1 cut(s) 431
TaqI TCGA 5 cut(s) 43, 110, 137, 224, 234
TfiI GAWTC 2 cut(s) 45, 407
Tru1I TTAA 1 cut(s) 323
Tru9I TTAA 1 cut(s) 323
TseI GCWGC 1 cut(s) 332
TspDTI ATGAA 2 cut(s) 357, 387
XapI RAATTY 4 cut(s) 27, 38, 273, 345
XmnI GAANNNNTTC 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.