Rmu_sc0005075.1_g000008

Zinc knuckle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005075.1
OG0025591
Physical Location & Seq
Reverse (-)
39642 .. 39911
270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005075.1_g000008.1.cds

Sequence Viewer

Length: 270 bp
atgcaatcgctgaaaaccgtaattcagcaacaccatcccccattcatctttttgagtgagattaaggttcatgattgggattacatgaatgaactgagatttaagattgggtaccttaactataagctagttttcagtgtggggcaagaaggaggcgtcgcactattctgggatgaaggccttgatgtgcatcatcgatcagagtcgctgcaccatatagacgtacaggtgcacgcgacagatggcactagtgtcacatggagactgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

89

Amino Acids

10.43

Weight (kDa)

6.0

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025591)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8417703.1_g000001 Rmu_sc0005075.1_g000008
rosa_samantha Rh2DG338200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 111
AccB1I GGYRCC 1 cut(s) 111
AccII CGCG 1 cut(s) 236
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 2 cut(s) 113, 225
AhlI ACTAGT 1 cut(s) 248
AluBI AGCT 1 cut(s) 127
AluI AGCT 1 cut(s) 127
Alw21I GWGCWC 1 cut(s) 234
Alw26I GTCTC 1 cut(s) 256
Alw44I GTGCAC 1 cut(s) 230
AoxI GGCC 1 cut(s) 178
ApaLI GTGCAC 1 cut(s) 230
ApeKI GCWGC 1 cut(s) 208
Asp718I GGTACC 1 cut(s) 111
BaeGI GKGCMC 1 cut(s) 234
BanI GGYRCC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 1 cut(s) 195
BccI CCATC 2 cut(s) 42, 236
BcoDI GTCTC 1 cut(s) 256
BcuI ACTAGT 1 cut(s) 248
BfaI CTAG 2 cut(s) 128, 249
BisI GCNGC 1 cut(s) 209
BlsI GCNGC 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 113
BmsI GCATC 1 cut(s) 199
Bsa29I ATCGAT 1 cut(s) 196
BsaBI GATNNNNATC 1 cut(s) 189
BsaHI GRCGYC 1 cut(s) 156
Bse8I GATNNNNATC 1 cut(s) 189
BseCI ATCGAT 1 cut(s) 196
BseGI GGATG 2 cut(s) 34, 178
BseJI GATNNNNATC 1 cut(s) 189
BseMII CTCAG 1 cut(s) 86
BseSI GKGCMC 1 cut(s) 234
BseXI GCAGC 1 cut(s) 195
BsgI GTGCAG 1 cut(s) 194
Bsh1236I CGCG 1 cut(s) 236
BshFI GGCC 1 cut(s) 180
BshNI GGYRCC 1 cut(s) 111
BshVI ATCGAT 1 cut(s) 196
BsiHKAI GWGCWC 1 cut(s) 234
BsmAI GTCTC 1 cut(s) 256
BsnI GGCC 1 cut(s) 180
Bsp1286I GDGCHC 1 cut(s) 234
Bsp143I GATC 1 cut(s) 197
BspANI GGCC 1 cut(s) 180
BspCNI CTCAG 1 cut(s) 87
BspDI ATCGAT 1 cut(s) 196
BspFNI CGCG 1 cut(s) 236
BspHI TCATGA 1 cut(s) 70
BspLI GGNNCC 1 cut(s) 113
BspT107I GGYRCC 1 cut(s) 111
BssMI GATC 1 cut(s) 197
BssNI GRCGYC 1 cut(s) 156
Bst4CI ACNGT 2 cut(s) 19, 267
BstACI GRCGYC 1 cut(s) 156
BstC8I GCNNGC 1 cut(s) 234
BstDEI CTNAG 1 cut(s) 95
BstF5I GGATG 2 cut(s) 34, 178
BstFNI CGCG 1 cut(s) 236
BstKTI GATC 1 cut(s) 200
BstMAI GTCTC 1 cut(s) 256
BstMBI GATC 1 cut(s) 197
BstSLI GKGCMC 1 cut(s) 234
BstUI CGCG 1 cut(s) 236
BstV1I GCAGC 1 cut(s) 195
Bsu15I ATCGAT 1 cut(s) 196
BsuRI GGCC 1 cut(s) 180
BsuTUI ATCGAT 1 cut(s) 196
BtsCI GGATG 2 cut(s) 34, 178
BtsIMutI CAGTG 1 cut(s) 142
Cac8I GCNNGC 1 cut(s) 234
CciI TCATGA 1 cut(s) 70
ClaI ATCGAT 1 cut(s) 196
CseI GACGC 1 cut(s) 145
Csp6I GTAC 2 cut(s) 112, 224
CviAII CATG 3 cut(s) 71, 85, 258
CviJI RGCY 2 cut(s) 127, 180
CviKI_1 RGCY 2 cut(s) 127, 180
CviQI GTAC 2 cut(s) 112, 224
DdeI CTNAG 1 cut(s) 95
DpnI GATC 1 cut(s) 199
DpnII GATC 1 cut(s) 197
Eco147I AGGCCT 1 cut(s) 180
FaeI CATG 3 cut(s) 74, 88, 261
FaiI YATR 6 cut(s) 72, 86, 123, 216, 218, 259
FatI CATG 3 cut(s) 70, 84, 257
Fnu4HI GCNGC 1 cut(s) 209
FokI GGATG 2 cut(s) 21, 185
Fsp4HI GCNGC 1 cut(s) 209
FspBI CTAG 2 cut(s) 128, 249
GluI GCNGC 1 cut(s) 209
HaeIII GGCC 1 cut(s) 180
HgaI GACGC 1 cut(s) 145
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 3 cut(s) 74, 88, 261
HinfI GANTC 1 cut(s) 203
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 1 cut(s) 202
Hpy188III TCNNGA 1 cut(s) 71
Hpy8I GTNNAC 1 cut(s) 232
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 2 cut(s) 143, 170
HpyCH4III ACNGT 2 cut(s) 19, 267
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 4 cut(s) 4, 190, 211, 232
HpyF3I CTNAG 1 cut(s) 95
HpySE526I ACGT 1 cut(s) 222
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 3 cut(s) 74, 88, 261
KpnI GGTACC 1 cut(s) 115
Kzo9I GATC 1 cut(s) 197
LpnPI CCDG 2 cut(s) 154, 212
Lsp1109I GCAGC 1 cut(s) 195
LweI GCATC 1 cut(s) 199
MaeI CTAG 2 cut(s) 128, 249
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 253
MalI GATC 1 cut(s) 199
MboI GATC 1 cut(s) 197
MhlI GDGCHC 1 cut(s) 234
MluCI AATT 1 cut(s) 21
MlyI GAGTC 1 cut(s) 212
MnlI CCTC 1 cut(s) 146
MseI TTAA 3 cut(s) 63, 102, 117
MvnI CGCG 1 cut(s) 236
NdeII GATC 1 cut(s) 197
NlaIII CATG 3 cut(s) 74, 88, 261
NlaIV GGNNCC 1 cut(s) 113
NmuCI GTSAC 1 cut(s) 253
PagI TCATGA 1 cut(s) 70
PceI AGGCCT 1 cut(s) 180
PkrI GCNGC 1 cut(s) 210
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
PspN4I GGNNCC 1 cut(s) 113
RsaI GTAC 2 cut(s) 113, 225
RsaNI GTAC 2 cut(s) 112, 224
SaqAI TTAA 3 cut(s) 63, 102, 117
SatI GCNGC 1 cut(s) 209
Sau3AI GATC 1 cut(s) 197
SchI GAGTC 1 cut(s) 212
SduI GDGCHC 1 cut(s) 234
SetI ASST 5 cut(s) 69, 117, 129, 225, 231
SfaNI GCATC 1 cut(s) 199
SpeI ACTAGT 1 cut(s) 248
Sse9I AATT 1 cut(s) 21
SseBI AGGCCT 1 cut(s) 180
SspMI CTAG 2 cut(s) 128, 249
StuI AGGCCT 1 cut(s) 180
TaaI ACNGT 2 cut(s) 19, 267
TaiI ACGT 1 cut(s) 225
TaqI TCGA 1 cut(s) 196
TasI AATT 1 cut(s) 21
Tru1I TTAA 3 cut(s) 63, 102, 117
Tru9I TTAA 3 cut(s) 63, 102, 117
TscAI CASTG 1 cut(s) 142
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 1 cut(s) 208
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 5 cut(s) 34, 59, 101, 105, 189
TspRI CASTG 1 cut(s) 142
VneI GTGCAC 1 cut(s) 230
XspI CTAG 2 cut(s) 128, 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.