Rmu_sc0005212.1_g000003

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005212.1
OG0025593
Physical Location & Seq
Forward (+)
16946 .. 17896
951 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 792 bp
atgctcaaatcttccattgtgtttatttgtgagactaagattcaggataagcaagttttcgagcgactgcataaggcgctagggtttccccattctcgattcattgatatggaggtgggcagtggaggtattgatgatccaaagtggagattcacggctttccacggttacccaatcattggggatgccaatgagatccttcatgctggggagaagttggggggtcgtgtactctgtgaggctcagctacagggatttagagatgtggtggattactgtgaactggttgatcttggttttacaggaagcccttatatgtggagcaacctggagacaaagattagtctggatcgagctttaggaacgcagtcatggttggatctgtttgggtattcgaaggtggtaaacctcccaccgagccacttggaccatgtaccggtgttgttgcgagtcagtagtatacctcttgagcagcgacaaaaatttcatagattcagatttgaaagcttctggttgcagaacccgaggtgtgatgaggtgcttcatgatcgatggcggaccaattttactggtcgacctatgtttcaagtggtgaagaagctgcaacatactagcaaagctttggatgtttggcaaaggagtacgttttcagatcgtaaacttcagatgcagtctgttcgtgaaaggttggaggctattttagggcttcctacatccgaggagttgaatgaggagagtagaatgttacaccttcagttggagcaccttctctcacaggatgaggcctactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

263

Amino Acids

30.59

Weight (kDa)

6.42

Isoelectric Point (pI)

40.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0025593)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8063360.1_g000001 Rmu_sc0005212.1_g000003 Rmu_sc0011745.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 179
AccI GTMKAC 2 cut(s) 460, 574
AciI CCGC 1 cut(s) 556
AclWI GGATC 4 cut(s) 131, 190, 357, 387
AcsI RAATTY 1 cut(s) 482
AcuI CTGAAG 2 cut(s) 647, 737
AfaI GTAC 3 cut(s) 231, 435, 643
AfiI CCNNNNNNNGG 3 cut(s) 179, 436, 757
AgeI ACCGGT 1 cut(s) 436
AgsI TTSAA 3 cut(s) 503, 587, 727
AjnI CCWGG 1 cut(s) 327
AluBI AGCT 5 cut(s) 247, 356, 507, 601, 620
AluI AGCT 5 cut(s) 247, 356, 507, 601, 620
Alw21I GWGCWC 1 cut(s) 765
Alw26I GTCTC 2 cut(s) 26, 326
AlwI GGATC 4 cut(s) 131, 190, 357, 387
Ama87I CYCGRG 1 cut(s) 523
AoxI GGCC 1 cut(s) 783
ApeKI GCWGC 2 cut(s) 472, 601
ApoI RAATTY 1 cut(s) 482
AsiGI ACCGGT 1 cut(s) 436
AspLEI GCGC 1 cut(s) 79
AspS9I GGNCC 2 cut(s) 427, 558
AsuHPI GGTGA 1 cut(s) 604
AsuII TTCGAA 1 cut(s) 395
AvaI CYCGRG 1 cut(s) 523
AvaII GGWCC 2 cut(s) 427, 558
Bbv12I GWGCWC 1 cut(s) 765
BbvI GCAGC 2 cut(s) 484, 588
BccI CCATC 1 cut(s) 546
BceAI ACGGC 1 cut(s) 171
BcgI CGANNNNNNTGC 2 cut(s) 659, 693
BciT130I CCWGG 1 cut(s) 329
BcoDI GTCTC 2 cut(s) 26, 326
BfaI CTAG 3 cut(s) 80, 612, 790
BfmI CTRYAG 1 cut(s) 248
BfoI RGCGCY 1 cut(s) 80
BisI GCNGC 2 cut(s) 473, 602
BlpI GCTNAGC 1 cut(s) 243
BlsI GCNGC 2 cut(s) 474, 603
Bme1390I CCNGG 1 cut(s) 329
Bme18I GGWCC 2 cut(s) 427, 558
BmeT110I CYCGRG 1 cut(s) 523
BmgT120I GGNCC 2 cut(s) 427, 558
BmrFI CCNGG 1 cut(s) 329
BmsI GCATC 2 cut(s) 175, 657
BpmI CTGGAG 1 cut(s) 350
Bpu1102I GCTNAGC 1 cut(s) 243
Bpu14I TTCGAA 1 cut(s) 395
BpuEI CTTGAG 1 cut(s) 488
Bsa29I ATCGAT 1 cut(s) 550
BsaJI CCNNGG 3 cut(s) 163, 524, 717
BsaWI WCCGGW 1 cut(s) 436
Bsc4I CCNNNNNNNGG 3 cut(s) 179, 436, 757
Bse118I RCCGGY 1 cut(s) 436
Bse1I ACTGG 2 cut(s) 288, 574
BseBI CCWGG 1 cut(s) 329
BseCI ATCGAT 1 cut(s) 550
BseDI CCNNGG 3 cut(s) 163, 524, 717
BseGI GGATG 4 cut(s) 190, 631, 713, 784
BseLI CCNNNNNNNGG 3 cut(s) 179, 436, 757
BseMII CTCAG 1 cut(s) 257
BseNI ACTGG 2 cut(s) 288, 574
BseRI GAGGAG 2 cut(s) 734, 746
BseXI GCAGC 2 cut(s) 484, 588
BseYI CCCAGC 1 cut(s) 206
BshFI GGCC 1 cut(s) 785
BshTI ACCGGT 1 cut(s) 436
BshVI ATCGAT 1 cut(s) 550
BsiHKAI GWGCWC 1 cut(s) 765
BsiHKCI CYCGRG 1 cut(s) 523
BsiSI CCGG 1 cut(s) 437
BslI CCNNNNNNNGG 3 cut(s) 179, 436, 757
BsmAI GTCTC 2 cut(s) 26, 326
BsnI GGCC 1 cut(s) 785
BsoBI CYCGRG 1 cut(s) 523
Bsp119I TTCGAA 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 765
Bsp143I GATC 7 cut(s) 136, 195, 289, 349, 379, 547, 652
Bsp1720I GCTNAGC 1 cut(s) 243
BspACI CCGC 1 cut(s) 556
BspANI GGCC 1 cut(s) 785
BspCNI CTCAG 1 cut(s) 256
BspDI ATCGAT 1 cut(s) 550
BspHI TCATGA 1 cut(s) 544
BspPI GGATC 4 cut(s) 131, 190, 357, 387
BspT104I TTCGAA 1 cut(s) 395
BsrFI RCCGGY 1 cut(s) 436
BsrI ACTGG 2 cut(s) 288, 574
BssAI RCCGGY 1 cut(s) 436
BssECI CCNNGG 3 cut(s) 163, 524, 717
BssMI GATC 7 cut(s) 136, 195, 289, 349, 379, 547, 652
BssNAI GTATAC 1 cut(s) 461
Bst1107I GTATAC 1 cut(s) 461
Bst2UI CCWGG 1 cut(s) 329
Bst4CI ACNGT 2 cut(s) 167, 278
BstBI TTCGAA 1 cut(s) 395
BstDEI CTNAG 2 cut(s) 36, 243
BstDSI CCRYGG 1 cut(s) 163
BstEII GGTNACC 1 cut(s) 167
BstF5I GGATG 4 cut(s) 190, 631, 713, 784
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 1 cut(s) 79
BstKTI GATC 7 cut(s) 139, 198, 292, 352, 382, 550, 655
BstMAI GTCTC 2 cut(s) 26, 326
BstMBI GATC 7 cut(s) 136, 195, 289, 349, 379, 547, 652
BstMWI GCNNNNNNNGC 1 cut(s) 76
BstNI CCWGG 1 cut(s) 329
BstPI GGTNACC 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 327
BstSFI CTRYAG 1 cut(s) 248
BstV1I GCAGC 2 cut(s) 484, 588
BstX2I RGATCY 2 cut(s) 195, 379
BstYI RGATCY 2 cut(s) 195, 379
BstZ17I GTATAC 1 cut(s) 461
Bsu15I ATCGAT 1 cut(s) 550
BsuRI GGCC 1 cut(s) 785
BsuTUI ATCGAT 1 cut(s) 550
BtgI CCRYGG 1 cut(s) 163
BtsCI GGATG 4 cut(s) 190, 631, 713, 784
BtsI GCAGTG 1 cut(s) 127
BtsIMutI CAGTG 1 cut(s) 127
CciI TCATGA 1 cut(s) 544
CfoI GCGC 1 cut(s) 79
Cfr10I RCCGGY 1 cut(s) 436
Cfr13I GGNCC 2 cut(s) 427, 558
ClaI ATCGAT 1 cut(s) 550
Csp6I GTAC 3 cut(s) 230, 434, 642
CspAI ACCGGT 1 cut(s) 436
CviAII CATG 4 cut(s) 203, 372, 431, 545
CviQI GTAC 3 cut(s) 230, 434, 642
DdeI CTNAG 2 cut(s) 36, 243
DpnI GATC 7 cut(s) 138, 197, 291, 351, 381, 549, 654
DpnII GATC 7 cut(s) 136, 195, 289, 349, 379, 547, 652
EciI GGCGGA 1 cut(s) 571
Eco147I AGGCCT 1 cut(s) 785
Eco47I GGWCC 2 cut(s) 427, 558
Eco57I CTGAAG 2 cut(s) 647, 737
Eco88I CYCGRG 1 cut(s) 523
Eco91I GGTNACC 1 cut(s) 167
EcoO65I GGTNACC 1 cut(s) 167
EcoRII CCWGG 1 cut(s) 327
FaeI CATG 4 cut(s) 206, 375, 434, 548
FatI CATG 4 cut(s) 202, 371, 430, 544
FblI GTMKAC 2 cut(s) 460, 574
Fnu4HI GCNGC 2 cut(s) 473, 602
FokI GGATG 3 cut(s) 197, 638, 700
Fsp4HI GCNGC 2 cut(s) 473, 602
FspBI CTAG 3 cut(s) 80, 612, 790
GlaI GCGC 1 cut(s) 78
GluI GCNGC 2 cut(s) 473, 602
GsaI CCCAGC 1 cut(s) 210
GsuI CTGGAG 1 cut(s) 350
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 1 cut(s) 785
HapII CCGG 1 cut(s) 437
HhaI GCGC 1 cut(s) 79
Hin1II CATG 4 cut(s) 206, 375, 434, 548
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HincII GTYRAC 1 cut(s) 575
HindII GTYRAC 1 cut(s) 575
HindIII AAGCTT 2 cut(s) 505, 618
HinfI GANTC 5 cut(s) 40, 99, 150, 450, 492
HpaII CCGG 1 cut(s) 437
HphI GGTGA 1 cut(s) 604
Hpy166II GTNNAC 6 cut(s) 230, 281, 406, 461, 575, 659
Hpy188I TCNGA 4 cut(s) 497, 652, 666, 718
Hpy188III TCNNGA 6 cut(s) 44, 96, 347, 467, 545, 680
Hpy8I GTNNAC 6 cut(s) 230, 281, 406, 461, 575, 659
HpyAV CCTTC 4 cut(s) 209, 391, 761, 776
HpyCH4III ACNGT 2 cut(s) 167, 278
HpyCH4IV ACGT 1 cut(s) 644
HpyCH4V TGCA 4 cut(s) 70, 517, 604, 670
HpyF10VI GCNNNNNNNGC 1 cut(s) 76
HpyF3I CTNAG 2 cut(s) 36, 243
HpySE526I ACGT 1 cut(s) 644
Hsp92II CATG 4 cut(s) 206, 375, 434, 548
HspAI GCGC 1 cut(s) 77
Kzo9I GATC 7 cut(s) 136, 195, 289, 349, 379, 547, 652
LmnI GCTCC 2 cut(s) 321, 760
Lsp1109I GCAGC 2 cut(s) 484, 588
LweI GCATC 2 cut(s) 175, 657
MaeI CTAG 3 cut(s) 80, 612, 790
MaeII ACGT 1 cut(s) 644
MaeIII GTNAC 2 cut(s) 167, 744
MalI GATC 7 cut(s) 138, 197, 291, 351, 381, 549, 654
MboI GATC 7 cut(s) 136, 195, 289, 349, 379, 547, 652
MboII GAAGA 2 cut(s) 3, 607
MflI RGATCY 2 cut(s) 195, 379
MhlI GDGCHC 1 cut(s) 765
MluCI AATT 2 cut(s) 482, 562
MlyI GAGTC 1 cut(s) 459
MmeI TCCRAC 3 cut(s) 357, 669, 738
MslI CAYNNNNRTG 1 cut(s) 107
MspI CCGG 1 cut(s) 437
MspR9I CCNGG 1 cut(s) 329
MvaI CCWGG 1 cut(s) 329
MwoI GCNNNNNNNGC 1 cut(s) 76
NdeII GATC 7 cut(s) 136, 195, 289, 349, 379, 547, 652
NlaIII CATG 4 cut(s) 206, 375, 434, 548
NspV TTCGAA 1 cut(s) 395
PagI TCATGA 1 cut(s) 544
PceI AGGCCT 1 cut(s) 785
PfeI GAWTC 4 cut(s) 40, 99, 150, 492
PflMI CCANNNNNTGG 1 cut(s) 179
PinAI ACCGGT 1 cut(s) 436
PkrI GCNGC 2 cut(s) 474, 603
PleI GAGTC 1 cut(s) 458
PpsI GAGTC 1 cut(s) 458
Psp6I CCWGG 1 cut(s) 327
PspEI GGTNACC 1 cut(s) 167
PspFI CCCAGC 1 cut(s) 206
PspGI CCWGG 1 cut(s) 327
PspPI GGNCC 2 cut(s) 427, 558
PsuI RGATCY 2 cut(s) 195, 379
RsaI GTAC 3 cut(s) 231, 435, 643
RsaNI GTAC 3 cut(s) 230, 434, 642
RseI CAYNNNNRTG 1 cut(s) 107
SalI GTCGAC 1 cut(s) 573
SatI GCNGC 2 cut(s) 473, 602
Sau3AI GATC 7 cut(s) 136, 195, 289, 349, 379, 547, 652
Sau96I GGNCC 2 cut(s) 427, 558
SchI GAGTC 1 cut(s) 459
ScrFI CCNGG 1 cut(s) 329
SduI GDGCHC 1 cut(s) 765
SfaNI GCATC 2 cut(s) 175, 657
SfcI CTRYAG 1 cut(s) 248
SfuI TTCGAA 1 cut(s) 395
SinI GGWCC 2 cut(s) 427, 558
SmiMI CAYNNNNRTG 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 467
SmoI CTYRAG 1 cut(s) 467
Sse9I AATT 2 cut(s) 482, 562
SseBI AGGCCT 1 cut(s) 785
SsiI CCGC 1 cut(s) 556
SspMI CTAG 3 cut(s) 80, 612, 790
StuI AGGCCT 1 cut(s) 785
StyD4I CCNGG 1 cut(s) 327
TaaI ACNGT 2 cut(s) 167, 278
TaiI ACGT 1 cut(s) 647
TaqI TCGA 6 cut(s) 60, 97, 352, 395, 550, 574
TasI AATT 2 cut(s) 482, 562
TatI WGTACW 1 cut(s) 229
TfiI GAWTC 4 cut(s) 40, 99, 150, 492
TscAI CASTG 1 cut(s) 127
TseI GCWGC 2 cut(s) 472, 601
TspDTI ATGAA 4 cut(s) 91, 191, 476, 533
TspRI CASTG 1 cut(s) 127
Van91I CCANNNNNTGG 1 cut(s) 179
VpaK11BI GGWCC 2 cut(s) 427, 558
XapI RAATTY 1 cut(s) 482
XmiI GTMKAC 2 cut(s) 460, 574
XspI CTAG 3 cut(s) 80, 612, 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.