Rmu_sc0005287.1_g000003

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005287.1
OG0025594
Physical Location & Seq
Reverse (-)
4551 .. 5512
962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005287.1_g000003.1.cds

Sequence Viewer

Length: 654 bp
atggccgaaccaagggtcgatcttacggcggtgtggcttttgaatgactatgcacgagtctctctgggatcagcggggctggaggcattatctggactcactattcatgatgtgggggatcgctttgtttttcagtttgaacgtgaaactgttgctgtcaaggggctacctttgattttacacaataagacggccttgcatctggttggatcatctctaggtcatgttatacggtttgatcaaactgcgttgcacaaaaaggaagaagaacaacgcattcgtctggctttcaatgttagacgccggattcagagttggaatctgtttgagttctcaccggtggtggttccggaactcacgttcatgtatgagaagatcaaaggcttatgtaggggatgtggtttttttgagcatgatgttaggggctgcgatctcttgttggaaaaagagaaggaggaggttttggctaaaccaccggaagctgctatggccatgttggcattgggttggagcatcatgcagcccagtcggaaactcaagaggcctgcattccaagatagtgggggagcttccactctttcggagcagccttttgctgggttcaatatgacaacatttctgaagacatgggcagcgagggaccttcaaaaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 Exo_endo_phos_2 RNase_H RVT_1 zf-CCHC_4

Protein Analysis

217

Amino Acids

24.49

Weight (kDa)

8.44

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025594)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0005287.1_g000003 Rmu_sc0029736.1_g000003
rosa_rugosa Rorug04G0330300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 349
AciI CCGC 2 cut(s) 29, 74
AclWI GGATC 3 cut(s) 76, 126, 217
AcoI YGGCCR 2 cut(s) 3, 489
AcuI CTGAAG 1 cut(s) 641
AcyI GRCGYC 1 cut(s) 301
AfiI CCNNNNNNNGG 2 cut(s) 12, 596
AgeI ACCGGT 1 cut(s) 337
AgsI TTSAA 5 cut(s) 43, 140, 292, 604, 647
AloI GAACNNNNNNTCC 2 cut(s) 344, 376
AluBI AGCT 2 cut(s) 482, 569
AluI AGCT 2 cut(s) 482, 569
Alw26I GTCTC 1 cut(s) 64
AlwI GGATC 3 cut(s) 76, 126, 217
Aor13HI TCCGGA 1 cut(s) 349
AoxI GGCC 4 cut(s) 3, 192, 489, 542
ApeKI GCWGC 5 cut(s) 426, 482, 520, 586, 632
ArsI GACNNNNNNTTYG 1 cut(s) 32
AsiGI ACCGGT 1 cut(s) 337
AspS9I GGNCC 1 cut(s) 640
AsuHPI GGTGA 1 cut(s) 327
AvaII GGWCC 1 cut(s) 640
BalI TGGCCA 1 cut(s) 491
BauI CACGAG 1 cut(s) 54
BbsI GAAGAC 1 cut(s) 629
BbvI GCAGC 5 cut(s) 413, 469, 532, 598, 644
BceAI ACGGC 2 cut(s) 42, 207
BcgI CGANNNNNNTGC 2 cut(s) 509, 543
BclI TGATCA 1 cut(s) 238
BcoDI GTCTC 1 cut(s) 64
BfaI CTAG 1 cut(s) 218
BglI GCCNNNNNGGC 1 cut(s) 497
BisI GCNGC 5 cut(s) 427, 483, 521, 587, 633
BlsI GCNGC 5 cut(s) 428, 484, 522, 588, 634
Bme18I GGWCC 1 cut(s) 640
BmgT120I GGNCC 1 cut(s) 640
BmiI GGNNCC 2 cut(s) 348, 641
BmrI ACTGGG 1 cut(s) 519
BmsI GCATC 2 cut(s) 208, 522
BmuI ACTGGG 1 cut(s) 519
BpiI GAAGAC 1 cut(s) 629
BpmI CTGGAG 1 cut(s) 101
BpuEI CTTGAG 1 cut(s) 521
BsaHI GRCGYC 1 cut(s) 301
BsaJI CCNNGG 1 cut(s) 11
BsaWI WCCGGW 3 cut(s) 337, 349, 475
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 596
Bse118I RCCGGY 1 cut(s) 337
Bse1I ACTGG 1 cut(s) 525
BseAI TCCGGA 1 cut(s) 349
BseDI CCNNGG 1 cut(s) 11
BseGI GGATG 1 cut(s) 401
BseLI CCNNNNNNNGG 2 cut(s) 12, 596
BseNI ACTGG 1 cut(s) 525
BseRI GAGGAG 1 cut(s) 470
BseXI GCAGC 5 cut(s) 413, 469, 532, 598, 644
BseYI CCCAGC 1 cut(s) 596
BshFI GGCC 4 cut(s) 5, 194, 491, 544
BshTI ACCGGT 1 cut(s) 337
BsiSI CCGG 4 cut(s) 304, 338, 350, 476
BslI CCNNNNNNNGG 2 cut(s) 12, 596
BsmAI GTCTC 1 cut(s) 64
BsmI GAATGC 2 cut(s) 276, 548
BsnI GGCC 4 cut(s) 5, 194, 491, 544
Bsp13I TCCGGA 1 cut(s) 349
Bsp143I GATC 7 cut(s) 19, 68, 118, 209, 238, 375, 430
BspACI CCGC 2 cut(s) 29, 74
BspANI GGCC 4 cut(s) 5, 194, 491, 544
BspEI TCCGGA 1 cut(s) 349
BspHI TCATGA 1 cut(s) 106
BspLI GGNNCC 2 cut(s) 348, 641
BspPI GGATC 3 cut(s) 76, 126, 217
BsrFI RCCGGY 1 cut(s) 337
BsrI ACTGG 1 cut(s) 525
BssAI RCCGGY 1 cut(s) 337
BssECI CCNNGG 1 cut(s) 11
BssMI GATC 7 cut(s) 19, 68, 118, 209, 238, 375, 430
BssNI GRCGYC 1 cut(s) 301
BssSI CACGAG 1 cut(s) 54
BssT1I CCWWGG 1 cut(s) 11
Bst2BI CACGAG 1 cut(s) 54
Bst4CI ACNGT 2 cut(s) 151, 234
BstACI GRCGYC 1 cut(s) 301
BstC8I GCNNGC 1 cut(s) 546
BstF5I GGATG 1 cut(s) 401
BstKTI GATC 7 cut(s) 22, 71, 121, 212, 241, 378, 433
BstMAI GTCTC 1 cut(s) 64
BstMBI GATC 7 cut(s) 19, 68, 118, 209, 238, 375, 430
BstMWI GCNNNNNNNGC 2 cut(s) 488, 497
BstV1I GCAGC 5 cut(s) 413, 469, 532, 598, 644
BstV2I GAAGAC 1 cut(s) 629
BstXI CCANNNNNNTGG 1 cut(s) 560
BsuRI GGCC 4 cut(s) 5, 194, 491, 544
BtsCI GGATG 1 cut(s) 401
Cac8I GCNNGC 1 cut(s) 546
CciI TCATGA 1 cut(s) 106
Cfr10I RCCGGY 1 cut(s) 337
Cfr13I GGNCC 1 cut(s) 640
CseI GACGC 1 cut(s) 309
CspAI ACCGGT 1 cut(s) 337
CviAII CATG 7 cut(s) 107, 224, 364, 413, 493, 517, 627
DpnI GATC 7 cut(s) 21, 70, 120, 211, 240, 377, 432
DpnII GATC 7 cut(s) 19, 68, 118, 209, 238, 375, 430
EaeI YGGCCR 2 cut(s) 3, 489
Eco130I CCWWGG 1 cut(s) 11
Eco147I AGGCCT 1 cut(s) 544
Eco47I GGWCC 1 cut(s) 640
Eco57I CTGAAG 1 cut(s) 641
EcoO109I RGGNCCY 1 cut(s) 640
EcoT14I CCWWGG 1 cut(s) 11
ErhI CCWWGG 1 cut(s) 11
FaeI CATG 7 cut(s) 110, 227, 367, 416, 496, 520, 630
FalI AAGNNNNNCTT 2 cut(s) 179, 211
FatI CATG 7 cut(s) 106, 223, 363, 412, 492, 516, 626
FauI CCCGC 1 cut(s) 67
FbaI TGATCA 1 cut(s) 238
Fnu4HI GCNGC 5 cut(s) 427, 483, 521, 587, 633
FokI GGATG 1 cut(s) 408
Fsp4HI GCNGC 5 cut(s) 427, 483, 521, 587, 633
FspBI CTAG 1 cut(s) 218
GluI GCNGC 5 cut(s) 427, 483, 521, 587, 633
GsaI CCCAGC 1 cut(s) 600
GsuI CTGGAG 1 cut(s) 101
HaeIII GGCC 4 cut(s) 5, 194, 491, 544
HapII CCGG 4 cut(s) 304, 338, 350, 476
HgaI GACGC 1 cut(s) 309
Hin1I GRCGYC 1 cut(s) 301
Hin1II CATG 7 cut(s) 110, 227, 367, 416, 496, 520, 630
HinfI GANTC 4 cut(s) 57, 96, 307, 319
HpaII CCGG 4 cut(s) 304, 338, 350, 476
HphI GGTGA 1 cut(s) 327
Hpy188I TCNGA 4 cut(s) 312, 531, 583, 621
Hpy188III TCNNGA 4 cut(s) 93, 107, 350, 538
HpyAV CCTTC 2 cut(s) 445, 653
HpyCH4III ACNGT 2 cut(s) 151, 234
HpyCH4IV ACGT 2 cut(s) 142, 359
HpyCH4V TGCA 5 cut(s) 53, 199, 253, 520, 548
HpyF10VI GCNNNNNNNGC 2 cut(s) 488, 497
HpySE526I ACGT 2 cut(s) 142, 359
Hsp92I GRCGYC 1 cut(s) 301
Hsp92II CATG 7 cut(s) 110, 227, 367, 416, 496, 520, 630
Kpn2I TCCGGA 1 cut(s) 349
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 7 cut(s) 19, 68, 118, 209, 238, 375, 430
LmnI GCTCC 3 cut(s) 510, 566, 583
Lsp1109I GCAGC 5 cut(s) 413, 469, 532, 598, 644
LweI GCATC 2 cut(s) 208, 522
MaeI CTAG 1 cut(s) 218
MaeII ACGT 2 cut(s) 142, 359
MalI GATC 7 cut(s) 21, 70, 120, 211, 240, 377, 432
MboI GATC 7 cut(s) 19, 68, 118, 209, 238, 375, 430
MboII GAAGA 4 cut(s) 275, 278, 385, 634
MlsI TGGCCA 1 cut(s) 491
MluNI TGGCCA 1 cut(s) 491
MlyI GAGTC 2 cut(s) 66, 90
MmeI TCCRAC 5 cut(s) 187, 296, 420, 488, 509
MnlI CCTC 5 cut(s) 76, 448, 451, 534, 630
Mox20I TGGCCA 1 cut(s) 491
MroI TCCGGA 1 cut(s) 349
MscI TGGCCA 1 cut(s) 491
MslI CAYNNNNRTG 1 cut(s) 362
Msp20I TGGCCA 1 cut(s) 491
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 4 cut(s) 304, 338, 350, 476
Mva1269I GAATGC 2 cut(s) 276, 548
MwoI GCNNNNNNNGC 2 cut(s) 488, 497
NdeII GATC 7 cut(s) 19, 68, 118, 209, 238, 375, 430
NlaIII CATG 7 cut(s) 110, 227, 367, 416, 496, 520, 630
NlaIV GGNNCC 2 cut(s) 348, 641
PagI TCATGA 1 cut(s) 106
PceI AGGCCT 1 cut(s) 544
PctI GAATGC 2 cut(s) 276, 548
PfeI GAWTC 2 cut(s) 307, 319
PinAI ACCGGT 1 cut(s) 337
PkrI GCNGC 5 cut(s) 428, 484, 522, 588, 634
PleI GAGTC 2 cut(s) 65, 90
PpsI GAGTC 2 cut(s) 65, 90
PpuMI RGGWCCY 1 cut(s) 640
Psp5II RGGWCCY 1 cut(s) 640
PspFI CCCAGC 1 cut(s) 596
PspN4I GGNNCC 2 cut(s) 348, 641
PspPI GGNCC 1 cut(s) 640
PspPPI RGGWCCY 1 cut(s) 640
RseI CAYNNNNRTG 1 cut(s) 362
SatI GCNGC 5 cut(s) 427, 483, 521, 587, 633
Sau3AI GATC 7 cut(s) 19, 68, 118, 209, 238, 375, 430
Sau96I GGNCC 1 cut(s) 640
SchI GAGTC 2 cut(s) 66, 90
SetI ASST 8 cut(s) 145, 172, 223, 362, 462, 484, 571, 645
SfaNI GCATC 2 cut(s) 208, 522
SgrAI CRCCGGYG 1 cut(s) 337
SinI GGWCC 1 cut(s) 640
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
SseBI AGGCCT 1 cut(s) 544
SsiI CCGC 2 cut(s) 29, 74
SspMI CTAG 1 cut(s) 218
StuI AGGCCT 1 cut(s) 544
StyI CCWWGG 1 cut(s) 11
TaaI ACNGT 2 cut(s) 151, 234
TaiI ACGT 2 cut(s) 145, 362
TaqI TCGA 1 cut(s) 18
TfiI GAWTC 2 cut(s) 307, 319
TseI GCWGC 5 cut(s) 426, 482, 520, 586, 632
TspDTI ATGAA 2 cut(s) 95, 352
VpaK11BI GGWCC 1 cut(s) 640
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
XspI CTAG 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.