Rmu_sc0005947.1_g000018

Stachyose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005947.1
OG0028378
Physical Location & Seq
Forward (+)
86827 .. 88406
1580 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1266 bp
atgtctgatctagctgtggagaaactagttgagggtgggattggacttgtccatcctgatcaagctggtgatttctataattctatgcactcttacctttctgaagctggtatcacaggagtcaaagtggatgttattcatactcttgaatatgtatctgaggaattcggaggccgtgttgagcttgcaaaggcttattacaaggggctgacttcttctcttgtaaagaacttcaatggaactggacttattgctagcatgcaacaatgcaatgatttcttcttccttggaacaaaacaaatctctattggaagagttggtgatgatttttggtttcaggatccaaatggagaccccatgggagtttactggctacaaggtgtccatatgatccattgtgcctacaacagcatgtggatgggtcaattcattcagcctgattgggatatgttccagtcagatcatctgtgtgccaaatttcatgccggatctagggctatatgtggaggtccggtgtatataagtgactcggttggtggtcacaattttgatcttattaggcagcttgtttacccagatggtaccatccccaagtgccagcattttgctcttccaactagggactgcctattcaagaaccccttatttgacagcaagactgctctcaaaatttggaatgtcaacaagtttggtggtgtgatgggagctttcaactgccaaggggctggttgggacccaaaagaaaagagaatcaaaggctactcccagtgctacaaggaaatcctctattcagtgcatgtttctgaaattgaatgggaccaaaagatggaagccgcccatttgggtaaggccgaagaatatgtggtctatctcaaccaggccaatgaactcttccaaatgacccccaaatccgatcccattcaaatggtgatccaaccatcttcgttcgagattttcagcttcgtgccagtacaaaagctcggctccaacaacaccaagtttgcgccgattggactgacaaacatgttcaacagtggaggcactatccaagagctggagtatacaagtggcatggaatcttatagtgcaaagatcaaggttaaaggtggaggcaacttcttggcctactcaagtcagtctccgaagaagtgttgtttgaatggtgctgtagtgacttttgagtggtctgcttctggtaaactgtccctgaatcttccttgggttgaagaagctgctggaatttctgatgtcgttcttgtgttttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006950 GO:0006979 GO:0008150 GO:0008378 GO:0009628 GO:0016740 GO:0016757 GO:0016758 GO:0047268 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

421

Amino Acids

46.68

Weight (kDa)

5.58

Isoelectric Point (pI)

27.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 1 - 404 1.9e-186 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028378)

Species Orthologous Gene IDs
malus_domestica MD15G1191000.v1.1
rosa_multiflora Rmu_sc0005947.1_g000018

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 581
AccB1I GGYRCC 1 cut(s) 581
AccB7I CCANNNNNTGG 2 cut(s) 826, 1054
AccI GTMKAC 1 cut(s) 1061
AciI CCGC 1 cut(s) 834
AclWI GGATC 6 cut(s) 335, 348, 385, 496, 908, 925
AcsI RAATTY 4 cut(s) 164, 476, 669, 1239
AcuI CTGAAG 1 cut(s) 123
AfaI GTAC 2 cut(s) 583, 972
AfiI CCNNNNNNNGG 3 cut(s) 492, 826, 1054
AflIII ACRYGT 1 cut(s) 1023
AgsI TTSAA 9 cut(s) 149, 235, 634, 712, 812, 923, 1030, 1159, 1226
AhlI ACTAGT 1 cut(s) 25
AjnI CCWGG 1 cut(s) 876
AjuI GAANNNNNNNTTGG 2 cut(s) 875, 907
Alw26I GTCTC 2 cut(s) 345, 1143
AlwI GGATC 6 cut(s) 335, 348, 385, 496, 908, 925
AoxI GGCC 4 cut(s) 172, 849, 879, 1122
ApeKI GCWGC 2 cut(s) 562, 1232
ApoI RAATTY 4 cut(s) 164, 476, 669, 1239
Asp718I GGTACC 1 cut(s) 581
AspLEI GCGC 1 cut(s) 1006
AspS9I GGNCC 3 cut(s) 509, 733, 817
AsuHPI GGTGA 3 cut(s) 80, 332, 940
AsuNHI GCTAGC 1 cut(s) 254
AvaII GGWCC 3 cut(s) 509, 733, 817
BamHI GGATCC 1 cut(s) 340
BanI GGYRCC 1 cut(s) 581
BbvI GCAGC 2 cut(s) 574, 1219
BccI CCATC 7 cut(s) 60, 412, 572, 593, 694, 820, 946
BceAI ACGGC 1 cut(s) 159
BciT130I CCWGG 1 cut(s) 878
BclI TGATCA 1 cut(s) 58
BcoDI GTCTC 2 cut(s) 345, 1143
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 5 cut(s) 11, 26, 255, 492, 618
BfmI CTRYAG 1 cut(s) 1167
BisI GCNGC 3 cut(s) 563, 834, 1233
BlsI GCNGC 3 cut(s) 564, 835, 1234
Bme1390I CCNGG 1 cut(s) 878
Bme18I GGWCC 3 cut(s) 509, 733, 817
BmgT120I GGNCC 3 cut(s) 509, 733, 817
BmiI GGNNCC 6 cut(s) 342, 583, 734, 735, 818, 985
BmrFI CCNGG 1 cut(s) 878
BmrI ACTGGG 1 cut(s) 760
BmtI GCTAGC 1 cut(s) 258
BmuI ACTGGG 1 cut(s) 760
BpmI CTGGAG 1 cut(s) 1076
BpuEI CTTGAG 1 cut(s) 1114
BsaI GGTCTC 1 cut(s) 345
BsaJI CCNNGG 4 cut(s) 286, 357, 718, 1217
BsaWI WCCGGW 1 cut(s) 511
BsaXI ACNNNNNCTCC 6 cut(s) 111, 141, 162, 192, 498, 528
Bsc4I CCNNNNNNNGG 3 cut(s) 492, 826, 1054
Bse1I ACTGG 5 cut(s) 247, 374, 454, 766, 968
Bse3DI GCAATG 1 cut(s) 277
BseBI CCWGG 1 cut(s) 878
BseDI CCNNGG 4 cut(s) 286, 357, 718, 1217
BseGI GGATG 4 cut(s) 52, 136, 423, 585
BseLI CCNNNNNNNGG 3 cut(s) 492, 826, 1054
BseMI GCAATG 1 cut(s) 277
BseMII CTCAG 1 cut(s) 150
BseNI ACTGG 5 cut(s) 247, 374, 454, 766, 968
BseXI GCAGC 2 cut(s) 574, 1219
BshFI GGCC 4 cut(s) 174, 851, 881, 1124
BshNI GGYRCC 1 cut(s) 581
BsiSI CCGG 2 cut(s) 486, 512
BslFI GGGAC 4 cut(s) 635, 746, 830, 1189
BslI CCNNNNNNNGG 3 cut(s) 492, 826, 1054
BsmAI GTCTC 2 cut(s) 345, 1143
BsmFI GGGAC 4 cut(s) 635, 746, 830, 1189
BsnI GGCC 4 cut(s) 174, 851, 881, 1124
Bso31I GGTCTC 1 cut(s) 345
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 1 cut(s) 834
BspANI GGCC 4 cut(s) 174, 851, 881, 1124
BspCNI CTCAG 1 cut(s) 151
BspLI GGNNCC 6 cut(s) 342, 583, 734, 735, 818, 985
BspOI GCTAGC 1 cut(s) 258
BspPI GGATC 6 cut(s) 335, 348, 385, 496, 908, 925
BspQI GCTCTTC 1 cut(s) 615
BspT107I GGYRCC 1 cut(s) 581
BspTNI GGTCTC 1 cut(s) 345
BsrDI GCAATG 1 cut(s) 277
BsrI ACTGG 5 cut(s) 247, 374, 454, 766, 968
BssECI CCNNGG 4 cut(s) 286, 357, 718, 1217
BssNAI GTATAC 1 cut(s) 1062
BssT1I CCWWGG 4 cut(s) 286, 357, 718, 1217
Bst1107I GTATAC 1 cut(s) 1062
Bst2UI CCWGG 1 cut(s) 878
Bst4CI ACNGT 2 cut(s) 1034, 1203
Bst6I CTCTTC 3 cut(s) 307, 615, 896
BstC8I GCNNGC 4 cut(s) 186, 256, 260, 599
BstDEI CTNAG 1 cut(s) 159
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 4 cut(s) 52, 136, 423, 585
BstHHI GCGC 1 cut(s) 1006
BstMAI GTCTC 2 cut(s) 345, 1143
BstNI CCWGG 1 cut(s) 878
BstNSI RCATGY 4 cut(s) 262, 415, 800, 1027
BstSCI CCNGG 1 cut(s) 876
BstSFI CTRYAG 1 cut(s) 1167
BstV1I GCAGC 2 cut(s) 574, 1219
BstX2I RGATCY 2 cut(s) 340, 488
BstXI CCANNNNNNTGG 2 cut(s) 725, 925
BstYI RGATCY 2 cut(s) 340, 488
BstZ17I GTATAC 1 cut(s) 1062
BsuRI GGCC 4 cut(s) 174, 851, 881, 1124
BtgI CCRYGG 1 cut(s) 357
BtsCI GGATG 4 cut(s) 52, 136, 423, 585
BtsIMutI CAGTG 3 cut(s) 773, 798, 1039
Cac8I GCNNGC 4 cut(s) 186, 256, 260, 599
CfoI GCGC 1 cut(s) 1006
Cfr13I GGNCC 3 cut(s) 509, 733, 817
Csp6I GTAC 2 cut(s) 582, 971
CspCI CAANNNNNGTGG 2 cut(s) 673, 708
CviAII CATG 7 cut(s) 259, 358, 412, 482, 797, 1024, 1072
CviQI GTAC 2 cut(s) 582, 971
DdeI CTNAG 1 cut(s) 159
Eam1104I CTCTTC 3 cut(s) 307, 615, 896
EarI CTCTTC 3 cut(s) 307, 615, 896
Eco130I CCWWGG 4 cut(s) 286, 357, 718, 1217
Eco31I GGTCTC 1 cut(s) 345
Eco47I GGWCC 3 cut(s) 509, 733, 817
Eco57I CTGAAG 1 cut(s) 123
EcoO109I RGGNCCY 1 cut(s) 733
EcoRI GAATTC 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 876
EcoT14I CCWWGG 4 cut(s) 286, 357, 718, 1217
ErhI CCWWGG 4 cut(s) 286, 357, 718, 1217
FaeI CATG 7 cut(s) 262, 361, 415, 485, 800, 1027, 1075
FalI AAGNNNNNCTT 2 cut(s) 626, 658
FaqI GGGAC 4 cut(s) 635, 746, 830, 1189
FatI CATG 7 cut(s) 258, 357, 411, 481, 796, 1023, 1071
FauNDI CATATG 1 cut(s) 387
FbaI TGATCA 1 cut(s) 58
FblI GTMKAC 1 cut(s) 1061
Fnu4HI GCNGC 3 cut(s) 563, 834, 1233
FokI GGATG 4 cut(s) 39, 143, 430, 572
Fsp4HI GCNGC 3 cut(s) 563, 834, 1233
FspBI CTAG 5 cut(s) 11, 26, 255, 492, 618
GlaI GCGC 1 cut(s) 1005
GluI GCNGC 3 cut(s) 563, 834, 1233
GsuI CTGGAG 1 cut(s) 1076
HaeIII GGCC 4 cut(s) 174, 851, 881, 1124
HapII CCGG 2 cut(s) 486, 512
HhaI GCGC 1 cut(s) 1006
Hin1II CATG 7 cut(s) 262, 361, 415, 485, 800, 1027, 1075
Hin6I GCGC 1 cut(s) 1004
HinP1I GCGC 1 cut(s) 1004
HincII GTYRAC 1 cut(s) 682
HindII GTYRAC 1 cut(s) 682
HinfI GANTC 5 cut(s) 120, 527, 750, 1076, 1210
HpaII CCGG 2 cut(s) 486, 512
HphI GGTGA 3 cut(s) 80, 332, 940
Hpy166II GTNNAC 5 cut(s) 367, 571, 682, 1062, 1199
Hpy188I TCNGA 9 cut(s) 7, 103, 160, 170, 460, 805, 913, 1143, 1246
Hpy188III TCNNGA 5 cut(s) 56, 146, 338, 634, 949
Hpy8I GTNNAC 5 cut(s) 367, 571, 682, 1062, 1199
HpyCH4III ACNGT 2 cut(s) 1034, 1203
HpyCH4V TGCA 6 cut(s) 88, 188, 262, 270, 796, 1088
HpyF3I CTNAG 1 cut(s) 159
Hsp92II CATG 7 cut(s) 262, 361, 415, 485, 800, 1027, 1075
HspAI GCGC 1 cut(s) 1004
KflI GGGWCCC 1 cut(s) 733
KpnI GGTACC 1 cut(s) 585
Ksp22I TGATCA 1 cut(s) 58
LguI GCTCTTC 1 cut(s) 615
LmnI GCTCC 2 cut(s) 704, 989
Lsp1109I GCAGC 2 cut(s) 574, 1219
MaeI CTAG 5 cut(s) 11, 26, 255, 492, 618
MaeIII GTNAC 3 cut(s) 524, 539, 1171
MflI RGATCY 2 cut(s) 340, 488
MluCI AATT 8 cut(s) 79, 164, 425, 476, 544, 669, 807, 1239
MlyI GAGTC 2 cut(s) 129, 521
MmeI TCCRAC 3 cut(s) 638, 958, 1011
MnlI CCTC 7 cut(s) 25, 154, 164, 500, 794, 1031, 1103
MseI TTAA 1 cut(s) 1101
MslI CAYNNNNRTG 3 cut(s) 416, 468, 923
MspI CCGG 2 cut(s) 486, 512
MspR9I CCNGG 1 cut(s) 878
MvaI CCWGG 1 cut(s) 878
NcoI CCATGG 1 cut(s) 357
NdeI CATATG 1 cut(s) 387
NheI GCTAGC 1 cut(s) 254
NlaIII CATG 7 cut(s) 262, 361, 415, 485, 800, 1027, 1075
NlaIV GGNNCC 6 cut(s) 342, 583, 734, 735, 818, 985
NmeAIII GCCGAG 1 cut(s) 960
NmuCI GTSAC 3 cut(s) 524, 539, 1171
NspI RCATGY 4 cut(s) 262, 415, 800, 1027
PaeI GCATGC 1 cut(s) 262
PciI ACATGT 1 cut(s) 1023
PciSI GCTCTTC 1 cut(s) 615
PfeI GAWTC 3 cut(s) 750, 1076, 1210
PflMI CCANNNNNTGG 2 cut(s) 826, 1054
PkrI GCNGC 3 cut(s) 564, 835, 1234
PleI GAGTC 2 cut(s) 128, 521
PpsI GAGTC 2 cut(s) 128, 521
PpuMI RGGWCCY 1 cut(s) 733
PscI ACATGT 1 cut(s) 1023
Psp5II RGGWCCY 1 cut(s) 733
Psp6I CCWGG 1 cut(s) 876
PspGI CCWGG 1 cut(s) 876
PspN4I GGNNCC 6 cut(s) 342, 583, 734, 735, 818, 985
PspPI GGNCC 3 cut(s) 509, 733, 817
PspPPI RGGWCCY 1 cut(s) 733
PsuI RGATCY 2 cut(s) 340, 488
RsaI GTAC 2 cut(s) 583, 972
RsaNI GTAC 2 cut(s) 582, 971
RseI CAYNNNNRTG 3 cut(s) 416, 468, 923
SapI GCTCTTC 1 cut(s) 615
SaqAI TTAA 1 cut(s) 1101
SatI GCNGC 3 cut(s) 563, 834, 1233
Sau96I GGNCC 3 cut(s) 509, 733, 817
SchI GAGTC 2 cut(s) 129, 521
ScrFI CCNGG 1 cut(s) 878
SfcI CTRYAG 1 cut(s) 1167
SinI GGWCC 3 cut(s) 509, 733, 817
SmiMI CAYNNNNRTG 3 cut(s) 416, 468, 923
SmlI CTYRAG 1 cut(s) 1129
SmoI CTYRAG 1 cut(s) 1129
SpeI ACTAGT 1 cut(s) 25
SphI GCATGC 1 cut(s) 262
Sse9I AATT 8 cut(s) 79, 164, 425, 476, 544, 669, 807, 1239
SsiI CCGC 1 cut(s) 834
SspMI CTAG 5 cut(s) 11, 26, 255, 492, 618
StyD4I CCNGG 1 cut(s) 876
StyI CCWWGG 4 cut(s) 286, 357, 718, 1217
TaaI ACNGT 2 cut(s) 1034, 1203
TaqI TCGA 1 cut(s) 948
TasI AATT 8 cut(s) 79, 164, 425, 476, 544, 669, 807, 1239
TatI WGTACW 1 cut(s) 970
TauI GCSGC 1 cut(s) 836
TfiI GAWTC 3 cut(s) 750, 1076, 1210
Tru1I TTAA 1 cut(s) 1101
Tru9I TTAA 1 cut(s) 1101
TscAI CASTG 3 cut(s) 773, 798, 1039
TseFI GTSAC 3 cut(s) 524, 539, 1171
TseI GCWGC 2 cut(s) 562, 1232
Tsp45I GTSAC 3 cut(s) 524, 539, 1171
TspDTI ATGAA 4 cut(s) 128, 418, 470, 900
TspRI CASTG 3 cut(s) 773, 798, 1039
Van91I CCANNNNNTGG 2 cut(s) 826, 1054
VpaK11BI GGWCC 3 cut(s) 509, 733, 817
XapI RAATTY 4 cut(s) 164, 476, 669, 1239
XceI RCATGY 4 cut(s) 262, 415, 800, 1027
XmiI GTMKAC 1 cut(s) 1061
XspI CTAG 5 cut(s) 11, 26, 255, 492, 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.