Rmu_sc0006221.1_g000006

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006221.1
OG0019373
Physical Location & Seq
Forward (+)
17238 .. 17945
708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006221.1_g000006.1.cds

Sequence Viewer

Length: 708 bp
atgccgaagttgagtcagaagaagtgccaatggaatgtccagccagcgactaaggaatatcgtgcccagttaccatcagaccctaaccctagaaacttgcaataccgacctaaacagacacctgttgaggaactataccgaagatataaggatccacatgaagagatgatcatggctgagggtatgtgtcgcctctgcactgatctcgatgtggatccccaagacattgtcatgttggttatgtcatggcacatgaaggcagccaccatgtgcgagttttcaaagcaagagttcttcaacggactaaaagccctaaagattgattccattgagaagttccgaagcgcgctaccgtctatgagatctgagctaaaggataaggacaagtttaaggaggtgtataattttacttttgactgggcaaaggaaaaggggcagaagtctttgcagctggatactgcaattggtatgtggcaattgctgtttgccaaaatgactccgaagtggccctatgttgatcaatggtgtgaattcttggaggagaggcacaagctggcaatacctaaggacacatggaatcaactattggattttgtaaggaatgtggatccatcactgtcgaattttgatgaagaaagagcttggccctgtcttgtcgacgattttgtggattacttgaaggagaaaggcttggtgcagactacttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding UBA_4

Protein Analysis

235

Amino Acids

27.94

Weight (kDa)

6.02

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019373)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0106621
rosa_laevigata RLG00000017522
rosa_multiflora Rmu_sc0006221.1_g000006
rosa_roxburghii Rroxscaffold_2G00136960
rosa_rugosa Rorug02G0136800
rosa_samantha Rh2BG199000 Rh2CG193100
rosa_wichuraiana Rw2G014760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 657
AccII CGCG 1 cut(s) 347
AclWI GGATC 6 cut(s) 146, 159, 209, 222, 602, 615
AcsI RAATTY 2 cut(s) 530, 622
AdeI CACNNNGTG 1 cut(s) 270
AgsI TTSAA 3 cut(s) 282, 298, 679
AjuI GAANNNNNNNTTGG 2 cut(s) 569, 601
AluBI AGCT 4 cut(s) 370, 451, 553, 641
AluI AGCT 4 cut(s) 370, 451, 553, 641
AlwI GGATC 6 cut(s) 146, 159, 209, 222, 602, 615
AoxI GGCC 2 cut(s) 506, 644
ApeKI GCWGC 2 cut(s) 260, 448
ApoI RAATTY 2 cut(s) 530, 622
AspLEI GCGC 2 cut(s) 347, 349
AspS9I GGNCC 2 cut(s) 507, 645
AxyI CCTNAGG 1 cut(s) 564
BaeGI GKGCMC 1 cut(s) 67
BamHI GGATCC 3 cut(s) 151, 214, 607
BbvCI CCTCAGC 1 cut(s) 177
BbvI GCAGC 2 cut(s) 272, 460
BccI CCATC 2 cut(s) 82, 619
BcgI CGANNNNNNTGC 2 cut(s) 187, 221
BciVI GTATCC 1 cut(s) 448
BclI TGATCA 2 cut(s) 168, 517
BfaI CTAG 1 cut(s) 90
BfuI GTATCC 1 cut(s) 448
BglII AGATCT 1 cut(s) 362
BisI GCNGC 2 cut(s) 261, 449
BlsI GCNGC 2 cut(s) 262, 450
BmgT120I GGNCC 2 cut(s) 507, 645
BmiI GGNNCC 3 cut(s) 153, 216, 609
BmrI ACTGGG 2 cut(s) 61, 427
BmuI ACTGGG 2 cut(s) 61, 427
Bpu10I CCTNAGC 1 cut(s) 177
BsaBI GATNNNNATC 1 cut(s) 213
Bse1I ACTGG 2 cut(s) 67, 422
Bse21I CCTNAGG 1 cut(s) 564
Bse8I GATNNNNATC 1 cut(s) 213
BseJI GATNNNNATC 1 cut(s) 213
BseMII CTCAG 2 cut(s) 168, 357
BseNI ACTGG 2 cut(s) 67, 422
BsePI GCGCGC 1 cut(s) 345
BseRI GAGGAG 1 cut(s) 554
BseSI GKGCMC 1 cut(s) 67
BseXI GCAGC 2 cut(s) 272, 460
BsgI GTGCAG 1 cut(s) 181
Bsh1236I CGCG 1 cut(s) 347
BshFI GGCC 2 cut(s) 508, 646
BsnI GGCC 2 cut(s) 508, 646
Bsp1286I GDGCHC 1 cut(s) 67
Bsp143I GATC 7 cut(s) 151, 168, 202, 214, 362, 517, 607
BspANI GGCC 2 cut(s) 508, 646
BspCNI CTCAG 2 cut(s) 169, 358
BspFNI CGCG 1 cut(s) 347
BspLI GGNNCC 3 cut(s) 153, 216, 609
BspPI GGATC 6 cut(s) 146, 159, 209, 222, 602, 615
BsrI ACTGG 2 cut(s) 67, 422
BssHII GCGCGC 1 cut(s) 345
BssMI GATC 7 cut(s) 151, 168, 202, 214, 362, 517, 607
Bst4CI ACNGT 2 cut(s) 354, 618
Bst6I CTCTTC 1 cut(s) 156
BstC8I GCNNGC 3 cut(s) 45, 347, 555
BstDEI CTNAG 5 cut(s) 51, 177, 366, 564, 705
BstFNI CGCG 1 cut(s) 347
BstHHI GCGC 2 cut(s) 347, 349
BstKTI GATC 7 cut(s) 154, 171, 205, 217, 365, 520, 610
BstMBI GATC 7 cut(s) 151, 168, 202, 214, 362, 517, 607
BstSLI GKGCMC 1 cut(s) 67
BstUI CGCG 1 cut(s) 347
BstV1I GCAGC 2 cut(s) 272, 460
BstX2I RGATCY 4 cut(s) 151, 214, 362, 607
BstYI RGATCY 4 cut(s) 151, 214, 362, 607
Bsu36I CCTNAGG 1 cut(s) 564
BsuI GTATCC 1 cut(s) 448
BsuRI GGCC 2 cut(s) 508, 646
BtsIMutI CAGTG 2 cut(s) 198, 614
Cac8I GCNNGC 3 cut(s) 45, 347, 555
CfoI GCGC 2 cut(s) 347, 349
Cfr13I GGNCC 2 cut(s) 507, 645
CviAII CATG 7 cut(s) 158, 172, 232, 246, 253, 268, 573
DdeI CTNAG 5 cut(s) 51, 177, 366, 564, 705
DpnI GATC 7 cut(s) 153, 170, 204, 216, 364, 519, 609
DpnII GATC 7 cut(s) 151, 168, 202, 214, 362, 517, 607
DraIII CACNNNGTG 1 cut(s) 270
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
Eco81I CCTNAGG 1 cut(s) 564
EcoRI GAATTC 1 cut(s) 530
FaeI CATG 7 cut(s) 161, 175, 235, 249, 256, 271, 576
FatI CATG 7 cut(s) 157, 171, 231, 245, 252, 267, 572
FbaI TGATCA 2 cut(s) 168, 517
FblI GTMKAC 1 cut(s) 657
Fnu4HI GCNGC 2 cut(s) 261, 449
Fsp4HI GCNGC 2 cut(s) 261, 449
FspBI CTAG 1 cut(s) 90
GlaI GCGC 2 cut(s) 346, 348
GluI GCNGC 2 cut(s) 261, 449
HaeIII GGCC 2 cut(s) 508, 646
HhaI GCGC 2 cut(s) 347, 349
Hin1II CATG 7 cut(s) 161, 175, 235, 249, 256, 271, 576
Hin6I GCGC 2 cut(s) 345, 347
HinP1I GCGC 2 cut(s) 345, 347
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HinfI GANTC 4 cut(s) 13, 323, 496, 577
Hpy166II GTNNAC 1 cut(s) 658
Hpy188I TCNGA 5 cut(s) 18, 79, 341, 367, 501
Hpy188III TCNNGA 1 cut(s) 206
Hpy8I GTNNAC 1 cut(s) 658
Hpy99I CGWCG 1 cut(s) 662
HpyAV CCTTC 2 cut(s) 250, 673
HpyCH4III ACNGT 2 cut(s) 354, 618
HpyCH4V TGCA 5 cut(s) 100, 198, 448, 461, 697
HpyF3I CTNAG 5 cut(s) 51, 177, 366, 564, 705
Hsp92II CATG 7 cut(s) 161, 175, 235, 249, 256, 271, 576
HspAI GCGC 2 cut(s) 345, 347
Ksp22I TGATCA 2 cut(s) 168, 517
Kzo9I GATC 7 cut(s) 151, 168, 202, 214, 362, 517, 607
LpnPI CCDG 8 cut(s) 53, 57, 80, 135, 403, 437, 539, 661
Lsp1109I GCAGC 2 cut(s) 272, 460
MaeI CTAG 1 cut(s) 90
MaeIII GTNAC 1 cut(s) 69
MalI GATC 7 cut(s) 153, 170, 204, 216, 364, 519, 609
MboI GATC 7 cut(s) 151, 168, 202, 214, 362, 517, 607
MboII GAAGA 5 cut(s) 31, 153, 173, 286, 644
MfeI CAATTG 2 cut(s) 462, 476
MflI RGATCY 4 cut(s) 151, 214, 362, 607
MhlI GDGCHC 1 cut(s) 67
MluCI AATT 5 cut(s) 403, 462, 476, 530, 622
MlyI GAGTC 2 cut(s) 22, 490
MnlI CCTC 6 cut(s) 121, 172, 203, 388, 532, 537
MseI TTAA 1 cut(s) 390
MslI CAYNNNNRTG 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 451
MunI CAATTG 2 cut(s) 462, 476
MvnI CGCG 1 cut(s) 347
NdeII GATC 7 cut(s) 151, 168, 202, 214, 362, 517, 607
NlaIII CATG 7 cut(s) 161, 175, 235, 249, 256, 271, 576
NlaIV GGNNCC 3 cut(s) 153, 216, 609
PauI GCGCGC 1 cut(s) 345
PfeI GAWTC 2 cut(s) 323, 577
PflFI GACNNNGTC 1 cut(s) 227
PkrI GCNGC 2 cut(s) 262, 450
PleI GAGTC 2 cut(s) 21, 490
PpsI GAGTC 2 cut(s) 21, 490
PspN4I GGNNCC 3 cut(s) 153, 216, 609
PspPI GGNCC 2 cut(s) 507, 645
PsuI RGATCY 4 cut(s) 151, 214, 362, 607
PsyI GACNNNGTC 1 cut(s) 227
PteI GCGCGC 1 cut(s) 345
PvuII CAGCTG 1 cut(s) 451
RseI CAYNNNNRTG 1 cut(s) 230
SalI GTCGAC 1 cut(s) 656
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 2 cut(s) 261, 449
Sau3AI GATC 7 cut(s) 151, 168, 202, 214, 362, 517, 607
Sau96I GGNCC 2 cut(s) 507, 645
SchI GAGTC 2 cut(s) 22, 490
SduI GDGCHC 1 cut(s) 67
SetI ASST 8 cut(s) 112, 124, 372, 399, 453, 555, 565, 643
SmiMI CAYNNNNRTG 1 cut(s) 230
Sse9I AATT 5 cut(s) 403, 462, 476, 530, 622
SspMI CTAG 1 cut(s) 90
TaaI ACNGT 2 cut(s) 354, 618
TaqI TCGA 3 cut(s) 207, 620, 657
TasI AATT 5 cut(s) 403, 462, 476, 530, 622
TfiI GAWTC 2 cut(s) 323, 577
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TscAI CASTG 2 cut(s) 205, 621
TseI GCWGC 2 cut(s) 260, 448
TspDTI ATGAA 3 cut(s) 174, 269, 645
TspGWI ACGGA 1 cut(s) 315
TspRI CASTG 2 cut(s) 205, 621
Tth111I GACNNNGTC 1 cut(s) 227
XapI RAATTY 2 cut(s) 530, 622
XmiI GTMKAC 1 cut(s) 657
XspI CTAG 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.