Rroxscaffold_2G00136960

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0019373
Physical Location & Seq
Forward (+)
74638850 .. 74639569
720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00136960.1

Sequence Viewer

Length: 720 bp
ATGCCGAAGTTGAGTCAGAAGAAGTGCCAAGGGAATGTCCAGCCAGCGACTAAGGAATATCGTGCCCAGTTACCATCAGACCCTAACCCTAGAAACTTGCAATACCGACCTATACAGACACCTGTTGAGGAACTATACCGAAGATATAAGGATCCACATGAAGAGATGATCATGGCTGAGGGTATGAGTCGCCTCTGCAGTGATCTCGATGTGGATCCCCAAGACATTGTCATGTTGGTTATGTCATGGCACATGAAGGCAGCCACCATGTGCGAGTTTTCAAAGCAAGAGTTCTTCAATGGACTAAAAGCCCTAAAGATTGATTCCATTGAGAAGTTCCGAAGCGCGCTACCGTCTATGAGATCTGAGCTGAAGGATAAGGACAAGTTTAAGGAGGTGTATAATTTTACTTTTGACTGGGCAAAGGAAAAGGGGCAGAAGTCTTTGCAGCTGGATACTGCAATTGGAATGTGGCAATTGCTGTTTGCCGAAATGACTCCGAAGTGGCCCTATGTTGATCAATGGTGTGAATTCTTGGAGGAGAGGCACAAGCTGGCAATACCTAAGGACACATGGAATCAACTATTGGATTTTGTAAGGAACGTGGACCCATCACTGTCGAATTTTGATGAAGAAAGAGCTTGGCCCTGTCTTGTCGACGATTTTGTGGATTACTTGAAGGAGAAAGGCCTGGTGCAAACTACTTGGGTAGTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding UBA_4

Protein Analysis

239

Amino Acids

28.25

Weight (kDa)

5.41

Isoelectric Point (pI)

33.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 113 - 225 8.9e-38 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019373)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0106621
rosa_laevigata RLG00000017522
rosa_multiflora Rmu_sc0006221.1_g000006
rosa_roxburghii Rroxscaffold_2G00136960
rosa_rugosa Rorug02G0136800
rosa_samantha Rh2BG199000 Rh2CG193100
rosa_wichuraiana Rw2G014760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 657
AccII CGCG 1 cut(s) 347
AclWI GGATC 4 cut(s) 146, 159, 209, 222
AcsI RAATTY 2 cut(s) 530, 622
AcuI CTGAAG 1 cut(s) 392
AdeI CACNNNGTG 1 cut(s) 270
AgsI TTSAA 3 cut(s) 282, 298, 679
AjnI CCWGG 1 cut(s) 690
AjuI GAANNNNNNNTTGG 2 cut(s) 569, 601
AluBI AGCT 4 cut(s) 370, 451, 553, 641
AluI AGCT 4 cut(s) 370, 451, 553, 641
AlwI GGATC 4 cut(s) 146, 159, 209, 222
AoxI GGCC 3 cut(s) 506, 644, 688
ApeKI GCWGC 2 cut(s) 260, 448
ApoI RAATTY 2 cut(s) 530, 622
AspLEI GCGC 2 cut(s) 347, 349
AspS9I GGNCC 3 cut(s) 507, 607, 645
AvaII GGWCC 1 cut(s) 607
AxyI CCTNAGG 1 cut(s) 564
BaeGI GKGCMC 1 cut(s) 67
BamHI GGATCC 2 cut(s) 151, 214
BbvCI CCTCAGC 1 cut(s) 177
BbvI GCAGC 2 cut(s) 272, 460
BccI CCATC 2 cut(s) 82, 619
BcgI CGANNNNNNTGC 2 cut(s) 187, 221
BciT130I CCWGG 1 cut(s) 692
BciVI GTATCC 1 cut(s) 448
BclI TGATCA 2 cut(s) 168, 517
BfaI CTAG 1 cut(s) 90
BfmI CTRYAG 1 cut(s) 196
BfuI GTATCC 1 cut(s) 448
BglII AGATCT 1 cut(s) 362
BisI GCNGC 2 cut(s) 261, 449
BlsI GCNGC 2 cut(s) 262, 450
Bme1390I CCNGG 1 cut(s) 692
Bme18I GGWCC 1 cut(s) 607
BmgT120I GGNCC 3 cut(s) 507, 607, 645
BmiI GGNNCC 3 cut(s) 153, 216, 609
BmrFI CCNGG 1 cut(s) 692
BmrI ACTGGG 2 cut(s) 61, 427
BmuI ACTGGG 2 cut(s) 61, 427
Bpu10I CCTNAGC 1 cut(s) 177
BsaBI GATNNNNATC 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 28
Bse1I ACTGG 2 cut(s) 67, 422
Bse21I CCTNAGG 1 cut(s) 564
Bse8I GATNNNNATC 1 cut(s) 213
BseBI CCWGG 1 cut(s) 692
BseDI CCNNGG 1 cut(s) 28
BseJI GATNNNNATC 1 cut(s) 213
BseMII CTCAG 2 cut(s) 168, 357
BseNI ACTGG 2 cut(s) 67, 422
BsePI GCGCGC 1 cut(s) 345
BseRI GAGGAG 1 cut(s) 554
BseSI GKGCMC 1 cut(s) 67
BseXI GCAGC 2 cut(s) 272, 460
Bsh1236I CGCG 1 cut(s) 347
BshFI GGCC 3 cut(s) 508, 646, 690
BsnI GGCC 3 cut(s) 508, 646, 690
Bsp1286I GDGCHC 1 cut(s) 67
Bsp143I GATC 6 cut(s) 151, 168, 202, 214, 362, 517
BspANI GGCC 3 cut(s) 508, 646, 690
BspCNI CTCAG 2 cut(s) 169, 358
BspFNI CGCG 1 cut(s) 347
BspLI GGNNCC 3 cut(s) 153, 216, 609
BspMAI CTGCAG 1 cut(s) 200
BspPI GGATC 4 cut(s) 146, 159, 209, 222
BsrI ACTGG 2 cut(s) 67, 422
BssECI CCNNGG 1 cut(s) 28
BssHII GCGCGC 1 cut(s) 345
BssMI GATC 6 cut(s) 151, 168, 202, 214, 362, 517
BssT1I CCWWGG 1 cut(s) 28
Bst2UI CCWGG 1 cut(s) 692
Bst4CI ACNGT 2 cut(s) 354, 618
Bst6I CTCTTC 1 cut(s) 156
BstC8I GCNNGC 3 cut(s) 45, 347, 555
BstDEI CTNAG 4 cut(s) 51, 177, 366, 564
BstFNI CGCG 1 cut(s) 347
BstHHI GCGC 2 cut(s) 347, 349
BstKTI GATC 6 cut(s) 154, 171, 205, 217, 365, 520
BstMBI GATC 6 cut(s) 151, 168, 202, 214, 362, 517
BstNI CCWGG 1 cut(s) 692
BstSCI CCNGG 1 cut(s) 690
BstSFI CTRYAG 1 cut(s) 196
BstSLI GKGCMC 1 cut(s) 67
BstUI CGCG 1 cut(s) 347
BstV1I GCAGC 2 cut(s) 272, 460
BstX2I RGATCY 3 cut(s) 151, 214, 362
BstYI RGATCY 3 cut(s) 151, 214, 362
Bsu36I CCTNAGG 1 cut(s) 564
BsuI GTATCC 1 cut(s) 448
BsuRI GGCC 3 cut(s) 508, 646, 690
BtsI GCAGTG 1 cut(s) 205
BtsIMutI CAGTG 2 cut(s) 205, 614
Cac8I GCNNGC 3 cut(s) 45, 347, 555
CfoI GCGC 2 cut(s) 347, 349
Cfr13I GGNCC 3 cut(s) 507, 607, 645
CviAII CATG 7 cut(s) 158, 172, 232, 246, 253, 268, 573
DdeI CTNAG 4 cut(s) 51, 177, 366, 564
DpnI GATC 6 cut(s) 153, 170, 204, 216, 364, 519
DpnII GATC 6 cut(s) 151, 168, 202, 214, 362, 517
DraIII CACNNNGTG 1 cut(s) 270
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
Eco130I CCWWGG 1 cut(s) 28
Eco147I AGGCCT 1 cut(s) 690
Eco47I GGWCC 1 cut(s) 607
Eco57I CTGAAG 1 cut(s) 392
Eco81I CCTNAGG 1 cut(s) 564
EcoRI GAATTC 1 cut(s) 530
EcoRII CCWGG 1 cut(s) 690
EcoT14I CCWWGG 1 cut(s) 28
ErhI CCWWGG 1 cut(s) 28
FaeI CATG 7 cut(s) 161, 175, 235, 249, 256, 271, 576
FatI CATG 7 cut(s) 157, 171, 231, 245, 252, 267, 572
FbaI TGATCA 2 cut(s) 168, 517
FblI GTMKAC 1 cut(s) 657
Fnu4HI GCNGC 2 cut(s) 261, 449
Fsp4HI GCNGC 2 cut(s) 261, 449
FspBI CTAG 1 cut(s) 90
GlaI GCGC 2 cut(s) 346, 348
GluI GCNGC 2 cut(s) 261, 449
HaeIII GGCC 3 cut(s) 508, 646, 690
HhaI GCGC 2 cut(s) 347, 349
Hin1II CATG 7 cut(s) 161, 175, 235, 249, 256, 271, 576
Hin6I GCGC 2 cut(s) 345, 347
HinP1I GCGC 2 cut(s) 345, 347
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HinfI GANTC 5 cut(s) 13, 187, 323, 496, 577
Hpy166II GTNNAC 2 cut(s) 607, 658
Hpy188I TCNGA 5 cut(s) 18, 79, 341, 367, 501
Hpy188III TCNNGA 1 cut(s) 206
Hpy8I GTNNAC 2 cut(s) 607, 658
Hpy99I CGWCG 1 cut(s) 662
HpyAV CCTTC 3 cut(s) 250, 367, 673
HpyCH4III ACNGT 2 cut(s) 354, 618
HpyCH4IV ACGT 1 cut(s) 603
HpyCH4V TGCA 5 cut(s) 100, 198, 448, 461, 697
HpyF3I CTNAG 4 cut(s) 51, 177, 366, 564
HpySE526I ACGT 1 cut(s) 603
Hsp92II CATG 7 cut(s) 161, 175, 235, 249, 256, 271, 576
HspAI GCGC 2 cut(s) 345, 347
Ksp22I TGATCA 2 cut(s) 168, 517
Kzo9I GATC 6 cut(s) 151, 168, 202, 214, 362, 517
Lsp1109I GCAGC 2 cut(s) 272, 460
MaeI CTAG 1 cut(s) 90
MaeII ACGT 1 cut(s) 603
MaeIII GTNAC 1 cut(s) 69
MalI GATC 6 cut(s) 153, 170, 204, 216, 364, 519
MboI GATC 6 cut(s) 151, 168, 202, 214, 362, 517
MboII GAAGA 5 cut(s) 31, 153, 173, 286, 644
MfeI CAATTG 2 cut(s) 462, 476
MflI RGATCY 3 cut(s) 151, 214, 362
MhlI GDGCHC 1 cut(s) 67
MluCI AATT 5 cut(s) 403, 462, 476, 530, 622
MlyI GAGTC 3 cut(s) 22, 196, 490
MnlI CCTC 6 cut(s) 121, 172, 203, 388, 532, 537
MseI TTAA 1 cut(s) 390
MslI CAYNNNNRTG 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 451
MspR9I CCNGG 1 cut(s) 692
MunI CAATTG 2 cut(s) 462, 476
MvaI CCWGG 1 cut(s) 692
MvnI CGCG 1 cut(s) 347
NdeII GATC 6 cut(s) 151, 168, 202, 214, 362, 517
NlaIII CATG 7 cut(s) 161, 175, 235, 249, 256, 271, 576
NlaIV GGNNCC 3 cut(s) 153, 216, 609
PauI GCGCGC 1 cut(s) 345
PceI AGGCCT 1 cut(s) 690
PfeI GAWTC 2 cut(s) 323, 577
PflFI GACNNNGTC 1 cut(s) 227
PkrI GCNGC 2 cut(s) 262, 450
PleI GAGTC 3 cut(s) 21, 195, 490
PpsI GAGTC 3 cut(s) 21, 195, 490
Psp6I CCWGG 1 cut(s) 690
PspGI CCWGG 1 cut(s) 690
PspN4I GGNNCC 3 cut(s) 153, 216, 609
PspPI GGNCC 3 cut(s) 507, 607, 645
PstI CTGCAG 1 cut(s) 200
PsuI RGATCY 3 cut(s) 151, 214, 362
PsyI GACNNNGTC 1 cut(s) 227
PteI GCGCGC 1 cut(s) 345
PvuII CAGCTG 1 cut(s) 451
RseI CAYNNNNRTG 1 cut(s) 230
SalI GTCGAC 1 cut(s) 656
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 2 cut(s) 261, 449
Sau3AI GATC 6 cut(s) 151, 168, 202, 214, 362, 517
Sau96I GGNCC 3 cut(s) 507, 607, 645
SchI GAGTC 3 cut(s) 22, 196, 490
ScrFI CCNGG 1 cut(s) 692
SduI GDGCHC 1 cut(s) 67
SetI ASST 9 cut(s) 112, 124, 372, 399, 453, 555, 565, 606, 643
SfcI CTRYAG 1 cut(s) 196
SinI GGWCC 1 cut(s) 607
SmiMI CAYNNNNRTG 1 cut(s) 230
Sse9I AATT 5 cut(s) 403, 462, 476, 530, 622
SseBI AGGCCT 1 cut(s) 690
SspMI CTAG 1 cut(s) 90
StuI AGGCCT 1 cut(s) 690
StyD4I CCNGG 1 cut(s) 690
StyI CCWWGG 1 cut(s) 28
TaaI ACNGT 2 cut(s) 354, 618
TaiI ACGT 1 cut(s) 606
TaqI TCGA 3 cut(s) 207, 620, 657
TasI AATT 5 cut(s) 403, 462, 476, 530, 622
TfiI GAWTC 2 cut(s) 323, 577
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TscAI CASTG 2 cut(s) 205, 621
TseI GCWGC 2 cut(s) 260, 448
TspDTI ATGAA 3 cut(s) 174, 269, 645
TspRI CASTG 2 cut(s) 205, 621
Tth111I GACNNNGTC 1 cut(s) 227
VpaK11BI GGWCC 1 cut(s) 607
XapI RAATTY 2 cut(s) 530, 622
XmiI GTMKAC 1 cut(s) 657
XspI CTAG 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.