Rmu_sc0007272.1_g000009

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007272.1
OG0031112
Physical Location & Seq
Forward (+)
43348 .. 50470
7123 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1428 bp
atgttcagcaggaatgacaggaattgtcgtagtctgtccccactatgtctcatattgatccccattaaagtgacgagatcacacaaatatgctgcaaacatgtttgcaaggaaggacatccctacgagaggatgtagcgccaccctatacggaggtaggcatggcaccaatgccaaggagagtggcactctggcattacaggccatggccttaattggatgcgtgggaggcccgtgggtcaaacctccaagcacccccattatcgtcggtagttttgatcctgaaaaggatcctctttgtccaaaggatgatgacgaagatgtgctagaggcaagaatgcattacttgagtacaataggcgcattattgtacttagctcaatgcacaagaccggacacctcatttagagacgatggattcagacccatcacacaccaggaacgtcgccaacactggcatgtgtccactatccccatcccaaaacgacatgtgttttggaaggttttgctgatgttggctaatagttatgatcaaacgaaggatcaggatctgctatctcacccgttgaagagcctagccaatcttgttgatacggtttccaacatctttgaacccagtaggacttctgtttcttcctcaagtatggatgattgtgttattattgaggaacctttaaggcccataggacagcgcccgatattaccttcatcagatatgcaaaagcgaagatcttcaaatcgcacagaccgcattgtttttaaactctttggaaaagacccgaatgaacttccatttgttctgcgatcacagattatcgactggttatcccatagtcctacagagattgaaagctatataaggccaagatgcatcattttgacaatttacctccgtctagaaaaatccacgtgggaagagctctgctgtcatctggaatccagtttgcaaagacttttagatgccaccaatgatcctttctggagaacaggatgggtgtatactaggatgcaacattttgttgcgtttatgtacaatggtcaagttgtcttagacacaaccttacctcttaaaagccaaaaaagttgcaggatatcaagcattaaacctattgcagtctctttatctgagagagcccagtttgttgttaaaggctttaaccttcctcattccaccacaaggttactatgtgctctagaagggaagtatctggcgcaagaagcttgtgatgacgtgatggatggtgctgatacaactgttgagcatgatgagctacagtgcatcagattctcctactctataccaaatgttactgggcgaggattcattgagtgggctaggagagccaaccatgtttatggtcaagtggaaattgatgaagtgcgtaatgagatcgcaagttatgtggtgaagaacatcctcaagctgtaa

Protein Analysis

475

Amino Acids

54.05

Weight (kDa)

8.32

Isoelectric Point (pI)

46.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_SPL7 PF26102 358 - 443 9.2e-28 SPL7-like, immunoglobulin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031112)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001009.1_g000030 Rmu_sc0007272.1_g000009

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 164
AccI GTMKAC 1 cut(s) 998
AciI CCGC 1 cut(s) 748
AclWI GGATC 7 cut(s) 52, 272, 284, 297, 549, 555, 965
AcvI CACGTG 1 cut(s) 909
AfaI GTAC 3 cut(s) 352, 371, 1031
AfiI CCNNNNNNNGG 2 cut(s) 128, 1176
AflIII ACRYGT 2 cut(s) 99, 487
AgsI TTSAA 4 cut(s) 568, 611, 735, 848
AjiI CACGTC 1 cut(s) 1231
AjnI CCWGG 1 cut(s) 435
AjuI GAANNNNNNNTTGG 2 cut(s) 959, 991
AluBI AGCT 6 cut(s) 377, 852, 919, 1220, 1270, 1423
AluI AGCT 6 cut(s) 377, 852, 919, 1220, 1270, 1423
Alw21I GWGCWC 2 cut(s) 921, 1192
Alw26I GTCTC 3 cut(s) 53, 402, 1120
AlwI GGATC 7 cut(s) 52, 272, 284, 297, 549, 555, 965
AlwNI CAGNNNCTG 1 cut(s) 550
AoxI GGCC 5 cut(s) 201, 207, 229, 677, 860
ApeKI GCWGC 1 cut(s) 92
ArsI GACNNNNNNTTYG 2 cut(s) 477, 509
Asp700I GAANNNNTTC 1 cut(s) 730
AspLEI GCGC 4 cut(s) 140, 362, 693, 1213
AspS9I GGNCC 2 cut(s) 230, 678
AsuHPI GGTGA 2 cut(s) 551, 1417
BaeI ACNNNNGTAYC 2 cut(s) 1239, 1272
BamHI GGATCC 1 cut(s) 289
BanI GGYRCC 1 cut(s) 164
BanII GRGCYC 2 cut(s) 921, 1135
BbrPI CACGTG 1 cut(s) 909
Bbv12I GWGCWC 2 cut(s) 921, 1192
BbvI GCAGC 1 cut(s) 79
BccI CCATC 6 cut(s) 407, 434, 482, 984, 1228, 1232
BciT130I CCWGG 1 cut(s) 437
BclI TGATCA 1 cut(s) 529
BcoDI GTCTC 3 cut(s) 53, 402, 1120
BfaI CTAG 6 cut(s) 326, 575, 896, 1002, 1193, 1335
BfmI CTRYAG 2 cut(s) 837, 1271
BfoI RGCGCY 2 cut(s) 141, 694
BglII AGATCT 1 cut(s) 728
BisI GCNGC 1 cut(s) 93
BlsI GCNGC 1 cut(s) 94
Bme1390I CCNGG 1 cut(s) 437
BmgBI CACGTC 1 cut(s) 1231
BmgT120I GGNCC 2 cut(s) 230, 678
BmiI GGNNCC 3 cut(s) 166, 291, 669
BmrFI CCNGG 1 cut(s) 437
BmrI ACTGGG 3 cut(s) 609, 1129, 1320
BmsI GCATC 6 cut(s) 209, 857, 879, 949, 996, 1287
BmuI ACTGGG 3 cut(s) 609, 1129, 1320
BplI GAGNNNNNCTC 2 cut(s) 172, 204
BpmI CTGGAG 1 cut(s) 1000
BpuEI CTTGAG 3 cut(s) 367, 622, 1403
BsaAI YACGTR 1 cut(s) 909
BsaBI GATNNNNATC 1 cut(s) 546
BsaJI CCNNGG 3 cut(s) 174, 204, 233
BsaWI WCCGGW 1 cut(s) 391
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 1176
Bse1I ACTGG 6 cut(s) 458, 615, 824, 939, 1135, 1315
Bse8I GATNNNNATC 1 cut(s) 546
BseBI CCWGG 1 cut(s) 437
BseDI CCNNGG 3 cut(s) 174, 204, 233
BseJI GATNNNNATC 1 cut(s) 546
BseLI CCNNNNNNNGG 2 cut(s) 128, 1176
BseMII CTCAG 1 cut(s) 1116
BseNI ACTGG 6 cut(s) 458, 615, 824, 939, 1135, 1315
BseXI GCAGC 1 cut(s) 79
BshFI GGCC 5 cut(s) 203, 209, 231, 679, 862
BshNI GGYRCC 1 cut(s) 164
BsiHKAI GWGCWC 2 cut(s) 921, 1192
BsiSI CCGG 1 cut(s) 392
BslFI GGGAC 1 cut(s) 22
BslI CCNNNNNNNGG 2 cut(s) 128, 1176
BsmAI GTCTC 3 cut(s) 53, 402, 1120
BsmBI CGTCTC 1 cut(s) 402
BsmFI GGGAC 1 cut(s) 22
BsmI GAATGC 1 cut(s) 342
BsnI GGCC 5 cut(s) 203, 209, 231, 679, 862
Bsp1286I GDGCHC 3 cut(s) 921, 1135, 1192
Bsp1407I TGTACA 1 cut(s) 1029
Bsp19I CCATGG 1 cut(s) 204
BspACI CCGC 1 cut(s) 748
BspANI GGCC 5 cut(s) 203, 209, 231, 679, 862
BspCNI CTCAG 1 cut(s) 1117
BspLI GGNNCC 3 cut(s) 166, 291, 669
BspPI GGATC 7 cut(s) 52, 272, 284, 297, 549, 555, 965
BspQI GCTCTTC 2 cut(s) 563, 909
BspT107I GGYRCC 1 cut(s) 164
BsrGI TGTACA 1 cut(s) 1029
BsrI ACTGG 6 cut(s) 458, 615, 824, 939, 1135, 1315
BssECI CCNNGG 3 cut(s) 174, 204, 233
BssNAI GTATAC 1 cut(s) 999
BssT1I CCWWGG 2 cut(s) 174, 204
Bst1107I GTATAC 1 cut(s) 999
Bst2UI CCWGG 1 cut(s) 437
Bst4CI ACNGT 3 cut(s) 595, 1255, 1275
Bst6I CTCTTC 2 cut(s) 563, 909
BstAUI TGTACA 1 cut(s) 1029
BstBAI YACGTR 1 cut(s) 909
BstDEI CTNAG 3 cut(s) 373, 1048, 1125
BstDSI CCRYGG 2 cut(s) 204, 233
BstENI CCTNNNNNAGG 1 cut(s) 126
BstH2I RGCGCY 2 cut(s) 141, 694
BstHHI GCGC 4 cut(s) 140, 362, 693, 1213
BstMAI GTCTC 3 cut(s) 53, 402, 1120
BstMWI GCNNNNNNNGC 6 cut(s) 200, 228, 747, 1217, 1267, 1340
BstNI CCWGG 1 cut(s) 437
BstNSI RCATGY 3 cut(s) 103, 461, 491
BstSCI CCNGG 1 cut(s) 435
BstSFI CTRYAG 2 cut(s) 837, 1271
BstV1I GCAGC 1 cut(s) 79
BstX2I RGATCY 3 cut(s) 289, 547, 728
BstXI CCANNNNNNTGG 1 cut(s) 1355
BstYI RGATCY 3 cut(s) 289, 547, 728
BstZ17I GTATAC 1 cut(s) 999
BsuRI GGCC 5 cut(s) 203, 209, 231, 679, 862
BtgI CCRYGG 2 cut(s) 204, 233
BtrI CACGTC 1 cut(s) 1231
BtsIMutI CAGTG 2 cut(s) 451, 1280
CaiI CAGNNNCTG 1 cut(s) 550
CfoI GCGC 4 cut(s) 140, 362, 693, 1213
Cfr13I GGNCC 2 cut(s) 230, 678
Csp6I GTAC 3 cut(s) 351, 370, 1030
CspCI CAANNNNNGTGG 4 cut(s) 163, 198, 1383, 1418
CviAII CATG 7 cut(s) 100, 161, 205, 458, 488, 1262, 1349
CviQI GTAC 3 cut(s) 351, 370, 1030
DdeI CTNAG 3 cut(s) 373, 1048, 1125
DraI TTTAAA 1 cut(s) 760
Eam1104I CTCTTC 2 cut(s) 563, 909
EarI CTCTTC 2 cut(s) 563, 909
Ecl136II GAGCTC 1 cut(s) 919
Eco130I CCWWGG 2 cut(s) 174, 204
Eco24I GRGCYC 2 cut(s) 921, 1135
Eco32I GATATC 1 cut(s) 1092
Eco53kI GAGCTC 1 cut(s) 919
Eco72I CACGTG 1 cut(s) 909
EcoICRI GAGCTC 1 cut(s) 919
EcoNI CCTNNNNNAGG 1 cut(s) 126
EcoRII CCWGG 1 cut(s) 435
EcoRV GATATC 1 cut(s) 1092
EcoT14I CCWWGG 2 cut(s) 174, 204
EcoT22I ATGCAT 2 cut(s) 342, 872
EcoT38I GRGCYC 2 cut(s) 921, 1135
ErhI CCWWGG 2 cut(s) 174, 204
Esp3I CGTCTC 1 cut(s) 402
FaeI CATG 7 cut(s) 103, 164, 208, 461, 491, 1265, 1352
FaqI GGGAC 1 cut(s) 22
FatI CATG 7 cut(s) 99, 160, 204, 457, 487, 1261, 1348
FbaI TGATCA 1 cut(s) 529
FblI GTMKAC 1 cut(s) 998
Fnu4HI GCNGC 1 cut(s) 93
FriOI GRGCYC 2 cut(s) 921, 1135
Fsp4HI GCNGC 1 cut(s) 93
FspBI CTAG 6 cut(s) 326, 575, 896, 1002, 1193, 1335
GlaI GCGC 4 cut(s) 139, 361, 692, 1212
GluI GCNGC 1 cut(s) 93
GsuI CTGGAG 1 cut(s) 1000
HaeII RGCGCY 2 cut(s) 141, 694
HaeIII GGCC 5 cut(s) 203, 209, 231, 679, 862
HapII CCGG 1 cut(s) 392
HhaI GCGC 4 cut(s) 140, 362, 693, 1213
Hin1II CATG 7 cut(s) 103, 164, 208, 461, 491, 1265, 1352
Hin6I GCGC 4 cut(s) 138, 360, 691, 1211
HinP1I GCGC 4 cut(s) 138, 360, 691, 1211
HindIII AAGCTT 1 cut(s) 1218
HinfI GANTC 4 cut(s) 417, 935, 1284, 1320
HpaII CCGG 1 cut(s) 392
HphI GGTGA 2 cut(s) 551, 1417
Hpy166II GTNNAC 2 cut(s) 465, 999
Hpy188I TCNGA 4 cut(s) 422, 712, 1126, 1283
Hpy188III TCNNGA 6 cut(s) 281, 545, 896, 932, 979, 1193
Hpy8I GTNNAC 2 cut(s) 465, 999
Hpy99I CGWCG 2 cut(s) 269, 447
HpyAV CCTTC 6 cut(s) 106, 493, 532, 714, 1169, 1190
HpyCH4III ACNGT 3 cut(s) 595, 1255, 1275
HpyCH4IV ACGT 3 cut(s) 442, 908, 1230
HpyF10VI GCNNNNNNNGC 6 cut(s) 200, 228, 747, 1217, 1267, 1340
HpyF3I CTNAG 3 cut(s) 373, 1048, 1125
HpySE526I ACGT 3 cut(s) 442, 908, 1230
Hsp92II CATG 7 cut(s) 103, 164, 208, 461, 491, 1265, 1352
HspAI GCGC 4 cut(s) 138, 360, 691, 1211
Ksp22I TGATCA 1 cut(s) 529
LguI GCTCTTC 2 cut(s) 563, 909
Lsp1109I GCAGC 1 cut(s) 79
LweI GCATC 6 cut(s) 209, 857, 879, 949, 996, 1287
MaeI CTAG 6 cut(s) 326, 575, 896, 1002, 1193, 1335
MaeII ACGT 3 cut(s) 442, 908, 1230
MaeIII GTNAC 3 cut(s) 70, 1179, 1306
MboII GAAGA 7 cut(s) 329, 580, 624, 723, 738, 926, 1420
MflI RGATCY 3 cut(s) 289, 547, 728
MhlI GDGCHC 3 cut(s) 921, 1135, 1192
MluCI AATT 4 cut(s) 22, 213, 882, 1368
MmeI TCCRAC 1 cut(s) 624
Mph1103I ATGCAT 2 cut(s) 342, 872
MroXI GAANNNNTTC 1 cut(s) 730
MseI TTAA 8 cut(s) 66, 212, 674, 759, 1068, 1101, 1146, 1155
MslI CAYNNNNRTG 4 cut(s) 68, 87, 456, 1353
MspI CCGG 1 cut(s) 392
MspR9I CCNGG 1 cut(s) 437
Mva1269I GAATGC 1 cut(s) 342
MvaI CCWGG 1 cut(s) 437
MwoI GCNNNNNNNGC 6 cut(s) 200, 228, 747, 1217, 1267, 1340
NcoI CCATGG 1 cut(s) 204
NlaIII CATG 7 cut(s) 103, 164, 208, 461, 491, 1265, 1352
NlaIV GGNNCC 3 cut(s) 166, 291, 669
NmuCI GTSAC 1 cut(s) 70
NsiI ATGCAT 2 cut(s) 342, 872
NspI RCATGY 3 cut(s) 103, 461, 491
PciI ACATGT 2 cut(s) 99, 487
PciSI GCTCTTC 2 cut(s) 563, 909
PctI GAATGC 1 cut(s) 342
PdmI GAANNNNTTC 1 cut(s) 730
PfeI GAWTC 4 cut(s) 417, 935, 1284, 1320
PkrI GCNGC 1 cut(s) 94
PmaCI CACGTG 1 cut(s) 909
PmlI CACGTG 1 cut(s) 909
Ppu21I YACGTR 1 cut(s) 909
PscI ACATGT 2 cut(s) 99, 487
Psp124BI GAGCTC 1 cut(s) 921
Psp6I CCWGG 1 cut(s) 435
PspCI CACGTG 1 cut(s) 909
PspGI CCWGG 1 cut(s) 435
PspN4I GGNNCC 3 cut(s) 166, 291, 669
PspPI GGNCC 2 cut(s) 230, 678
PstNI CAGNNNCTG 1 cut(s) 550
PsuI RGATCY 3 cut(s) 289, 547, 728
RsaI GTAC 3 cut(s) 352, 371, 1031
RsaNI GTAC 3 cut(s) 351, 370, 1030
RseI CAYNNNNRTG 4 cut(s) 68, 87, 456, 1353
SacI GAGCTC 1 cut(s) 921
SapI GCTCTTC 2 cut(s) 563, 909
SaqAI TTAA 8 cut(s) 66, 212, 674, 759, 1068, 1101, 1146, 1155
SatI GCNGC 1 cut(s) 93
Sau96I GGNCC 2 cut(s) 230, 678
ScrFI CCNGG 1 cut(s) 437
SduI GDGCHC 3 cut(s) 921, 1135, 1192
SfaNI GCATC 6 cut(s) 209, 857, 879, 949, 996, 1287
SfcI CTRYAG 2 cut(s) 837, 1271
SmiMI CAYNNNNRTG 4 cut(s) 68, 87, 456, 1353
SmlI CTYRAG 3 cut(s) 346, 637, 1418
SmoI CTYRAG 3 cut(s) 346, 637, 1418
Sse9I AATT 4 cut(s) 22, 213, 882, 1368
SsiI CCGC 1 cut(s) 748
SspMI CTAG 6 cut(s) 326, 575, 896, 1002, 1193, 1335
SstI GAGCTC 1 cut(s) 921
StyD4I CCNGG 1 cut(s) 435
StyI CCWWGG 2 cut(s) 174, 204
TaaI ACNGT 3 cut(s) 595, 1255, 1275
TaiI ACGT 3 cut(s) 445, 911, 1233
TaqI TCGA 1 cut(s) 816
TasI AATT 4 cut(s) 22, 213, 882, 1368
TatI WGTACW 3 cut(s) 350, 369, 1029
TfiI GAWTC 4 cut(s) 417, 935, 1284, 1320
Tru1I TTAA 8 cut(s) 66, 212, 674, 759, 1068, 1101, 1146, 1155
Tru9I TTAA 8 cut(s) 66, 212, 674, 759, 1068, 1101, 1146, 1155
TscAI CASTG 2 cut(s) 458, 1280
TseFI GTSAC 1 cut(s) 70
TseI GCWGC 1 cut(s) 92
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 4 cut(s) 696, 798, 1312, 1389
TspGWI ACGGA 2 cut(s) 165, 881
TspRI CASTG 2 cut(s) 458, 1280
XagI CCTNNNNNAGG 1 cut(s) 126
XbaI TCTAGA 2 cut(s) 895, 1192
XceI RCATGY 3 cut(s) 103, 461, 491
XmiI GTMKAC 1 cut(s) 998
XmnI GAANNNNTTC 1 cut(s) 730
XspI CTAG 6 cut(s) 326, 575, 896, 1002, 1193, 1335
Zsp2I ATGCAT 2 cut(s) 342, 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.