Rmu_sc0012409.1_g000002

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0012409.1
OG0031263
Physical Location & Seq
Forward (+)
678 .. 1223
546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0012409.1_g000002.1.cds

Sequence Viewer

Length: 546 bp
atgaggggatttcgcgaggcactagggtatgcagacctactggacctagggtttcacggcaccaagtctacatggtggaatgctgatatgaaacttaggttagacagggcggtttgctcgccttcttggtgcgatatttttggttatgcaaaactagtgcatcttcccccaagtgattcggatcatgtgcccatattactgcatgcaagcactattcctatacctcagcgtactcgaatccaaatattcaaatttgaagctttctgggttcaacacaaagtctatgatccgattgtgaagcaggcttggcagagcgatgttacgggggttcctttgtttagagttaccacaaagaacaaacatacaagaatttcgcttgataaatggaagaaagcagagttttgtgagagacagcagagcatgctgggtatccaggctagattagaagatctaatggagaccagggcgtgcaatgaggtacaggaggagaagaagttgctgatggagcgcctacaatgtcttctttcacaagaggaatctttctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

181

Amino Acids

21.15

Weight (kDa)

8.27

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031263)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003430.1_g000010 Rmu_sc0012409.1_g000002

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 59
AccI GTMKAC 1 cut(s) 68
AccII CGCG 1 cut(s) 15
AciI CCGC 1 cut(s) 110
AclWI GGATC 2 cut(s) 189, 281
AcsI RAATTY 2 cut(s) 251, 369
AfaI GTAC 2 cut(s) 232, 480
AgsI TTSAA 3 cut(s) 250, 257, 272
AhlI ACTAGT 1 cut(s) 154
AjnI CCWGG 2 cut(s) 432, 461
AluBI AGCT 1 cut(s) 260
AluI AGCT 1 cut(s) 260
Alw26I GTCTC 2 cut(s) 403, 452
AlwI GGATC 2 cut(s) 189, 281
ApoI RAATTY 2 cut(s) 251, 369
AspA2I CCTAGG 1 cut(s) 46
AspLEI GCGC 1 cut(s) 510
AspS9I GGNCC 1 cut(s) 43
AvaII GGWCC 1 cut(s) 43
AvrII CCTAGG 1 cut(s) 46
BaeGI GKGCMC 1 cut(s) 192
BanI GGYRCC 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 512
BbvCI CCTCAGC 1 cut(s) 225
BccI CCATC 1 cut(s) 496
BceAI ACGGC 1 cut(s) 73
BciT130I CCWGG 2 cut(s) 434, 463
BciVI GTATCC 1 cut(s) 440
BcoDI GTCTC 2 cut(s) 403, 452
BcuI ACTAGT 1 cut(s) 154
BfaI CTAG 5 cut(s) 23, 47, 155, 438, 544
BfoI RGCGCY 1 cut(s) 511
BfuI GTATCC 1 cut(s) 440
BglII AGATCT 1 cut(s) 448
BlnI CCTAGG 1 cut(s) 46
Bme1390I CCNGG 2 cut(s) 434, 463
Bme18I GGWCC 1 cut(s) 43
BmgT120I GGNCC 1 cut(s) 43
BmiI GGNNCC 2 cut(s) 61, 330
BmrFI CCNGG 2 cut(s) 434, 463
BmsI GCATC 1 cut(s) 169
BpiI GAAGAC 1 cut(s) 512
Bpu10I CCTNAGC 1 cut(s) 225
BsaBI GATNNNNATC 1 cut(s) 180
BsaI GGTCTC 1 cut(s) 452
BsaJI CCNNGG 2 cut(s) 46, 462
BsaXI ACNNNNNCTCC 2 cut(s) 479, 509
Bse1I ACTGG 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 478
Bse8I GATNNNNATC 1 cut(s) 180
BseBI CCWGG 2 cut(s) 434, 463
BseDI CCNNGG 2 cut(s) 46, 462
BseJI GATNNNNATC 1 cut(s) 180
BseMI GCAATG 1 cut(s) 478
BseMII CTCAG 1 cut(s) 239
BseNI ACTGG 1 cut(s) 45
BseRI GAGGAG 1 cut(s) 500
BseSI GKGCMC 1 cut(s) 192
BseYI CCCAGC 1 cut(s) 424
Bsh1236I CGCG 1 cut(s) 15
BshNI GGYRCC 1 cut(s) 59
BsmAI GTCTC 2 cut(s) 403, 452
BsmI GAATGC 1 cut(s) 85
Bso31I GGTCTC 1 cut(s) 452
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 3 cut(s) 181, 286, 448
Bsp68I TCGCGA 1 cut(s) 15
BspACI CCGC 1 cut(s) 110
BspCNI CTCAG 1 cut(s) 238
BspFNI CGCG 1 cut(s) 15
BspLI GGNNCC 2 cut(s) 61, 330
BspPI GGATC 2 cut(s) 189, 281
BspT107I GGYRCC 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 452
BsrDI GCAATG 1 cut(s) 478
BsrI ACTGG 1 cut(s) 45
BssECI CCNNGG 2 cut(s) 46, 462
BssMI GATC 3 cut(s) 181, 286, 448
BssT1I CCWWGG 1 cut(s) 46
Bst2UI CCWGG 2 cut(s) 434, 463
BstAPI GCANNNNNTGC 1 cut(s) 421
BstC8I GCNNGC 6 cut(s) 119, 204, 208, 303, 422, 469
BstDEI CTNAG 2 cut(s) 95, 225
BstFNI CGCG 1 cut(s) 15
BstH2I RGCGCY 1 cut(s) 511
BstHHI GCGC 1 cut(s) 510
BstKTI GATC 3 cut(s) 184, 289, 451
BstMAI GTCTC 2 cut(s) 403, 452
BstMBI GATC 3 cut(s) 181, 286, 448
BstMWI GCNNNNNNNGC 3 cut(s) 307, 421, 505
BstNI CCWGG 2 cut(s) 434, 463
BstNSI RCATGY 2 cut(s) 206, 424
BstSCI CCNGG 2 cut(s) 432, 461
BstSLI GKGCMC 1 cut(s) 192
BstUI CGCG 1 cut(s) 15
BstV2I GAAGAC 1 cut(s) 512
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BsuI GTATCC 1 cut(s) 440
BtgZI GCGATG 1 cut(s) 330
BtuMI TCGCGA 1 cut(s) 15
Cac8I GCNNGC 6 cut(s) 119, 204, 208, 303, 422, 469
CfoI GCGC 1 cut(s) 510
Cfr13I GGNCC 1 cut(s) 43
Csp6I GTAC 2 cut(s) 231, 479
CviAII CATG 4 cut(s) 72, 185, 203, 421
CviJI RGCY 3 cut(s) 260, 305, 437
CviKI_1 RGCY 3 cut(s) 260, 305, 437
CviQI GTAC 2 cut(s) 231, 479
DdeI CTNAG 2 cut(s) 95, 225
DpnI GATC 3 cut(s) 183, 288, 450
DpnII GATC 3 cut(s) 181, 286, 448
Eco130I CCWWGG 1 cut(s) 46
Eco31I GGTCTC 1 cut(s) 452
Eco47I GGWCC 1 cut(s) 43
EcoRII CCWGG 2 cut(s) 432, 461
EcoT14I CCWWGG 1 cut(s) 46
ErhI CCWWGG 1 cut(s) 46
FaeI CATG 4 cut(s) 75, 188, 206, 424
FatI CATG 4 cut(s) 71, 184, 202, 420
FblI GTMKAC 1 cut(s) 68
FspBI CTAG 5 cut(s) 23, 47, 155, 438, 544
GlaI GCGC 1 cut(s) 509
GsaI CCCAGC 1 cut(s) 428
HaeII RGCGCY 1 cut(s) 511
HhaI GCGC 1 cut(s) 510
Hin1II CATG 4 cut(s) 75, 188, 206, 424
Hin6I GCGC 1 cut(s) 508
HinP1I GCGC 1 cut(s) 508
HindIII AAGCTT 1 cut(s) 258
HinfI GANTC 3 cut(s) 176, 237, 536
Hpy166II GTNNAC 1 cut(s) 69
Hpy188I TCNGA 2 cut(s) 181, 291
Hpy188III TCNNGA 1 cut(s) 14
Hpy8I GTNNAC 1 cut(s) 69
HpyAV CCTTC 1 cut(s) 132
HpyCH4V TGCA 6 cut(s) 32, 149, 160, 202, 206, 471
HpyF10VI GCNNNNNNNGC 3 cut(s) 307, 421, 505
HpyF3I CTNAG 2 cut(s) 95, 225
Hsp92II CATG 4 cut(s) 75, 188, 206, 424
HspAI GCGC 1 cut(s) 508
Kzo9I GATC 3 cut(s) 181, 286, 448
LmnI GCTCC 1 cut(s) 505
LweI GCATC 1 cut(s) 169
MaeI CTAG 5 cut(s) 23, 47, 155, 438, 544
MaeIII GTNAC 2 cut(s) 319, 343
MalI GATC 3 cut(s) 183, 288, 450
MboI GATC 3 cut(s) 181, 286, 448
MboII GAAGA 5 cut(s) 155, 400, 458, 502, 512
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 2 cut(s) 251, 369
MnlI CCTC 5 cut(s) 10, 234, 469, 478, 526
MspR9I CCNGG 2 cut(s) 434, 463
Mva1269I GAATGC 1 cut(s) 85
MvaI CCWGG 2 cut(s) 434, 463
MvnI CGCG 1 cut(s) 15
MwoI GCNNNNNNNGC 3 cut(s) 307, 421, 505
NdeII GATC 3 cut(s) 181, 286, 448
NlaIII CATG 4 cut(s) 75, 188, 206, 424
NlaIV GGNNCC 2 cut(s) 61, 330
NruI TCGCGA 1 cut(s) 15
NspI RCATGY 2 cut(s) 206, 424
PaeI GCATGC 2 cut(s) 206, 424
PctI GAATGC 1 cut(s) 85
PfeI GAWTC 3 cut(s) 176, 237, 536
Psp6I CCWGG 2 cut(s) 432, 461
PspFI CCCAGC 1 cut(s) 424
PspGI CCWGG 2 cut(s) 432, 461
PspN4I GGNNCC 2 cut(s) 61, 330
PspPI GGNCC 1 cut(s) 43
PsuI RGATCY 1 cut(s) 448
RruI TCGCGA 1 cut(s) 15
RsaI GTAC 2 cut(s) 232, 480
RsaNI GTAC 2 cut(s) 231, 479
Sau3AI GATC 3 cut(s) 181, 286, 448
Sau96I GGNCC 1 cut(s) 43
ScrFI CCNGG 2 cut(s) 434, 463
SduI GDGCHC 1 cut(s) 192
SetI ASST 6 cut(s) 39, 48, 101, 226, 262, 480
SfaNI GCATC 1 cut(s) 169
SinI GGWCC 1 cut(s) 43
SpeI ACTAGT 1 cut(s) 154
SphI GCATGC 2 cut(s) 206, 424
Sse9I AATT 2 cut(s) 251, 369
SsiI CCGC 1 cut(s) 110
SspI AATATT 1 cut(s) 246
SspMI CTAG 5 cut(s) 23, 47, 155, 438, 544
StyD4I CCNGG 2 cut(s) 432, 461
StyI CCWWGG 1 cut(s) 46
TaqI TCGA 1 cut(s) 235
TasI AATT 2 cut(s) 251, 369
TfiI GAWTC 3 cut(s) 176, 237, 536
TspDTI ATGAA 1 cut(s) 104
VpaK11BI GGWCC 1 cut(s) 43
XapI RAATTY 2 cut(s) 251, 369
XceI RCATGY 2 cut(s) 206, 424
XmaJI CCTAGG 1 cut(s) 46
XmiI GTMKAC 1 cut(s) 68
XspI CTAG 5 cut(s) 23, 47, 155, 438, 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.