Rmu_sc0016036.1_g000001

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho- Glu-tRNA(Gln)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0016036.1
OG0021626
Physical Location & Seq
Reverse (-)
1 .. 2269
2269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0016036.1_g000001.1.cds

Sequence Viewer

Length: 1272 bp
atgctatccacactccaacctccgcgctcactctctcacttccgcactaaacccctcctcctccgccgtcacacctcccttaaacccctctcttctttatcctcacagcctgtcacccactccaccctcacagaccaatccaaatcccaaatcctctccacccgcgactccctcctctcccgccaaatcaccgccgccgagctcgccgagtccttcctcgcccgcctccgccgcaccgagccccacctccgctccttcctccacgtctccgacgccgtcctcgcccaggcccgcgacctcgacgacaagcttcgccggaatgaggaggtgggacccttggccggggtccttgtggcggtgaaggataacatatgtacggcggagatgccgtccacggcggggtccagggttttggagggctatacgccgccgtatgatgccaccgccgtcaggaagattaaggagctgggtgggattgtggtgggcaagactaatttggatgagtttgggatgggtagcaccaccgaaggctccgcctttcaggttttggatcttctgcatgggtcacctttactttatcagtttacttgtgggttctgtgaaactttatgtacggcggagatgccgtccacggcggggtccagggttttggagggctatacgccgccgtatgatgccaccgccgtcaggaagattaaggagctgggtgggattgtggtgggcaagactaatttggatgagtttgggatgggtagcaccaccgaaggctccgcctttcaggtgactgcaaacccatgggacacgacacgggtgccaggagggtcatcaggaggctcggctgcagctgtgtctgccaggcagagtatggtatcgttgggaagtgatactggtggaagtgtgaggcagccagcatcgttttgtggtgttgttgggttgaagcccacgtatgggcgtgtgtcgaggtttggacttatggcatatgcgtcatctcttgatgttattggctgcttgggaacatctgttgctgatgctgggattcttcttcatgcaatttctggtcatgatagacttgatgctaccagcagcaagcgtgaggtccccgaccttgcatctcaatttgtttccattagtttgctagagtctaaacctctgaaaggactaaaaattggtctgatccgtgaaactctcgatgatggtgttgatattggagtaacttctgcaattcgtactgctgcactgcatcttgaagaattaggatgctccgtaacagag

Protein Analysis

424

Amino Acids

44.88

Weight (kDa)

6.52

Isoelectric Point (pI)

38.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 811
AccBSI CCGCTC 1 cut(s) 252
AccII CGCG 3 cut(s) 25, 165, 294
AclWI GGATC 2 cut(s) 558, 1168
AcoI YGGCCR 1 cut(s) 339
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 3 cut(s) 376, 613, 1228
AgsI TTSAA 2 cut(s) 937, 1247
AjiI CACGTC 1 cut(s) 265
AjnI CCWGG 5 cut(s) 285, 404, 641, 814, 854
AluBI AGCT 5 cut(s) 202, 310, 466, 703, 845
AluI AGCT 5 cut(s) 202, 310, 466, 703, 845
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 1 cut(s) 271
AlwI GGATC 2 cut(s) 558, 1168
AoxI GGCC 2 cut(s) 288, 339
ApeKI GCWGC 6 cut(s) 839, 842, 904, 1005, 1083, 1232
AspLEI GCGC 1 cut(s) 27
AspS9I GGNCC 6 cut(s) 289, 332, 346, 402, 639, 1096
AsuC2I CCSGG 1 cut(s) 343
AsuHPI GGTGA 5 cut(s) 106, 181, 370, 558, 793
AvaII GGWCC 5 cut(s) 332, 346, 402, 639, 1096
BanI GGYRCC 1 cut(s) 811
BanII GRGCYC 2 cut(s) 204, 243
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 6 cut(s) 826, 854, 916, 992, 1095, 1219
BccI CCATC 3 cut(s) 505, 742, 1187
BcgI CGANNNNNNTGC 2 cut(s) 793, 827
BciT130I CCWGG 5 cut(s) 287, 406, 643, 816, 856
BcnI CCSGG 1 cut(s) 343
BcoDI GTCTC 1 cut(s) 271
BfaI CTAG 1 cut(s) 1136
BfmI CTRYAG 1 cut(s) 840
Bme1390I CCNGG 6 cut(s) 287, 343, 406, 643, 816, 856
Bme18I GGWCC 5 cut(s) 332, 346, 402, 639, 1096
BmgBI CACGTC 1 cut(s) 265
BmgT120I GGNCC 6 cut(s) 289, 332, 346, 402, 639, 1096
BmiI GGNNCC 9 cut(s) 333, 334, 347, 403, 532, 640, 769, 813, 1098
BmrFI CCNGG 6 cut(s) 287, 343, 406, 643, 816, 856
BpuMI CCSGG 1 cut(s) 343
BsaAI YACGTR 1 cut(s) 945
BsaHI GRCGYC 1 cut(s) 273
BsaJI CCNNGG 8 cut(s) 285, 336, 342, 393, 405, 630, 642, 794
BsaXI ACNNNNNCTCC 8 cut(s) 42, 72, 236, 266, 374, 404, 611, 641
Bse1I ACTGG 1 cut(s) 892
BseBI CCWGG 5 cut(s) 287, 406, 643, 816, 856
BseDI CCNNGG 8 cut(s) 285, 336, 342, 393, 405, 630, 642, 794
BseGI GGATG 5 cut(s) 505, 516, 742, 753, 1262
BseNI ACTGG 1 cut(s) 892
BseRI GAGGAG 4 cut(s) 47, 50, 164, 338
BseXI GCAGC 6 cut(s) 826, 854, 916, 992, 1095, 1219
BseYI CCCAGC 3 cut(s) 466, 703, 1031
BsgI GTGCAG 1 cut(s) 1218
Bsh1236I CGCG 3 cut(s) 25, 165, 294
BshFI GGCC 2 cut(s) 290, 341
BshNI GGYRCC 1 cut(s) 811
BsiHKAI GWGCWC 1 cut(s) 204
BsiSI CCGG 2 cut(s) 316, 342
BslFI GGGAC 3 cut(s) 345, 812, 1082
BsmAI GTCTC 1 cut(s) 271
BsmBI CGTCTC 1 cut(s) 271
BsmFI GGGAC 3 cut(s) 345, 812, 1082
BsnI GGCC 2 cut(s) 290, 341
Bsp1286I GDGCHC 2 cut(s) 204, 243
Bsp143I GATC 2 cut(s) 550, 1173
Bsp19I CCATGG 1 cut(s) 794
BspANI GGCC 2 cut(s) 290, 341
BspFNI CGCG 3 cut(s) 25, 165, 294
BspHI TCATGA 1 cut(s) 1060
BspLI GGNNCC 9 cut(s) 333, 334, 347, 403, 532, 640, 769, 813, 1098
BspMAI CTGCAG 1 cut(s) 844
BspPI GGATC 2 cut(s) 558, 1168
BspT107I GGYRCC 1 cut(s) 811
BsrBI CCGCTC 1 cut(s) 252
BsrI ACTGG 1 cut(s) 892
BssECI CCNNGG 8 cut(s) 285, 336, 342, 393, 405, 630, 642, 794
BssMI GATC 2 cut(s) 550, 1173
BssNI GRCGYC 1 cut(s) 273
BssT1I CCWWGG 2 cut(s) 336, 794
Bst2UI CCWGG 5 cut(s) 287, 406, 643, 816, 856
Bst6I CTCTTC 1 cut(s) 97
BstACI GRCGYC 1 cut(s) 273
BstBAI YACGTR 1 cut(s) 945
BstC8I GCNNGC 5 cut(s) 204, 223, 292, 909, 1088
BstDSI CCRYGG 3 cut(s) 393, 630, 794
BstEII GGTNACC 1 cut(s) 564
BstENI CCTNNNNNAGG 2 cut(s) 284, 1152
BstF5I GGATG 5 cut(s) 505, 516, 742, 753, 1262
BstFNI CGCG 3 cut(s) 25, 165, 294
BstHHI GCGC 1 cut(s) 27
BstKTI GATC 2 cut(s) 553, 1176
BstMAI GTCTC 1 cut(s) 271
BstMBI GATC 2 cut(s) 550, 1173
BstMWI GCNNNNNNNGC 4 cut(s) 203, 231, 281, 851
BstNI CCWGG 5 cut(s) 287, 406, 643, 816, 856
BstPI GGTNACC 1 cut(s) 564
BstSCI CCNGG 6 cut(s) 285, 341, 404, 641, 814, 854
BstSFI CTRYAG 1 cut(s) 840
BstUI CGCG 3 cut(s) 25, 165, 294
BstV1I GCAGC 6 cut(s) 826, 854, 916, 992, 1095, 1219
BstX2I RGATCY 1 cut(s) 550
BstXI CCANNNNNNTGG 2 cut(s) 412, 649
BstYI RGATCY 1 cut(s) 550
BsuRI GGCC 2 cut(s) 290, 341
BtgI CCRYGG 3 cut(s) 393, 630, 794
BtrI CACGTC 1 cut(s) 265
BtsCI GGATG 5 cut(s) 505, 516, 742, 753, 1262
BtsI GCAGTG 1 cut(s) 1235
BtsIMutI CAGTG 1 cut(s) 1235
Cac8I GCNNGC 5 cut(s) 204, 223, 292, 909, 1088
CciI TCATGA 1 cut(s) 1060
CfoI GCGC 1 cut(s) 27
Cfr13I GGNCC 6 cut(s) 289, 332, 346, 402, 639, 1096
CseI GACGC 2 cut(s) 281, 972
Csp6I GTAC 3 cut(s) 375, 612, 1227
CviAII CATG 4 cut(s) 560, 795, 1046, 1061
CviQI GTAC 3 cut(s) 375, 612, 1227
DpnI GATC 2 cut(s) 552, 1175
DpnII GATC 2 cut(s) 550, 1173
EaeI YGGCCR 1 cut(s) 339
Eam1104I CTCTTC 1 cut(s) 97
EarI CTCTTC 1 cut(s) 97
EciI GGCGGA 6 cut(s) 53, 218, 395, 523, 632, 760
Ecl136II GAGCTC 1 cut(s) 202
Eco130I CCWWGG 2 cut(s) 336, 794
Eco24I GRGCYC 2 cut(s) 204, 243
Eco47I GGWCC 5 cut(s) 332, 346, 402, 639, 1096
Eco53kI GAGCTC 1 cut(s) 202
Eco91I GGTNACC 1 cut(s) 564
EcoICRI GAGCTC 1 cut(s) 202
EcoNI CCTNNNNNAGG 2 cut(s) 284, 1152
EcoO109I RGGNCCY 3 cut(s) 332, 346, 1096
EcoO65I GGTNACC 1 cut(s) 564
EcoRII CCWGG 5 cut(s) 285, 404, 641, 814, 854
EcoT14I CCWWGG 2 cut(s) 336, 794
EcoT38I GRGCYC 2 cut(s) 204, 243
ErhI CCWWGG 2 cut(s) 336, 794
Esp3I CGTCTC 1 cut(s) 271
FaeI CATG 4 cut(s) 563, 798, 1049, 1064
FaqI GGGAC 3 cut(s) 345, 812, 1082
FatI CATG 4 cut(s) 559, 794, 1045, 1060
FauI CCCGC 6 cut(s) 170, 188, 230, 299, 391, 628
FauNDI CATATG 2 cut(s) 371, 979
FokI GGATG 4 cut(s) 512, 523, 749, 760
FriOI GRGCYC 2 cut(s) 204, 243
FspBI CTAG 1 cut(s) 1136
GlaI GCGC 1 cut(s) 26
GsaI CCCAGC 3 cut(s) 470, 707, 1035
HaeIII GGCC 2 cut(s) 290, 341
HapII CCGG 2 cut(s) 316, 342
HgaI GACGC 2 cut(s) 281, 972
HhaI GCGC 1 cut(s) 27
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 4 cut(s) 563, 798, 1049, 1064
Hin6I GCGC 1 cut(s) 25
HinP1I GCGC 1 cut(s) 25
HindIII AAGCTT 1 cut(s) 308
HinfI GANTC 4 cut(s) 167, 209, 1036, 1139
HpaII CCGG 2 cut(s) 316, 342
HphI GGTGA 5 cut(s) 106, 181, 370, 558, 793
Hpy166II GTNNAC 3 cut(s) 393, 585, 630
Hpy188I TCNGA 3 cut(s) 271, 1152, 1173
Hpy188III TCNNGA 7 cut(s) 451, 688, 828, 992, 1061, 1187, 1244
Hpy8I GTNNAC 3 cut(s) 393, 585, 630
Hpy99I CGWCG 2 cut(s) 275, 305
HpyAV CCTTC 5 cut(s) 223, 265, 355, 521, 758
HpyCH4IV ACGT 2 cut(s) 264, 944
HpyCH4V TGCA 8 cut(s) 559, 788, 842, 1049, 1109, 1220, 1235, 1240
HpyF10VI GCNNNNNNNGC 4 cut(s) 203, 231, 281, 851
HpySE526I ACGT 2 cut(s) 264, 944
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 4 cut(s) 563, 798, 1049, 1064
HspAI GCGC 1 cut(s) 25
KflI GGGWCCC 1 cut(s) 332
Kzo9I GATC 2 cut(s) 550, 1173
LmnI GCTCC 6 cut(s) 257, 463, 536, 700, 773, 1265
Lsp1109I GCAGC 6 cut(s) 826, 854, 916, 992, 1095, 1219
MaeI CTAG 1 cut(s) 1136
MaeII ACGT 2 cut(s) 264, 944
MaeIII GTNAC 6 cut(s) 68, 112, 564, 781, 1210, 1264
MalI GATC 2 cut(s) 552, 1175
MbiI CCGCTC 1 cut(s) 252
MboI GATC 2 cut(s) 550, 1173
MboII GAAGA 7 cut(s) 84, 466, 545, 703, 1031, 1034, 1259
MflI RGATCY 1 cut(s) 550
MhlI GDGCHC 2 cut(s) 204, 243
MluCI AATT 7 cut(s) 493, 730, 1050, 1115, 1164, 1221, 1250
MlyI GAGTC 3 cut(s) 161, 218, 1148
MmeI TCCRAC 2 cut(s) 40, 294
MseI TTAA 3 cut(s) 81, 459, 696
MspA1I CMGCKG 1 cut(s) 845
MspI CCGG 2 cut(s) 316, 342
MspR9I CCNGG 6 cut(s) 287, 343, 406, 643, 816, 856
MvaI CCWGG 5 cut(s) 287, 406, 643, 816, 856
MvnI CGCG 3 cut(s) 25, 165, 294
MwoI GCNNNNNNNGC 4 cut(s) 203, 231, 281, 851
NciI CCSGG 1 cut(s) 343
NcoI CCATGG 1 cut(s) 794
NdeI CATATG 2 cut(s) 371, 979
NdeII GATC 2 cut(s) 550, 1173
NlaIII CATG 4 cut(s) 563, 798, 1049, 1064
NlaIV GGNNCC 9 cut(s) 333, 334, 347, 403, 532, 640, 769, 813, 1098
NmeAIII GCCGAG 3 cut(s) 223, 232, 815
NmuCI GTSAC 4 cut(s) 68, 112, 564, 781
PagI TCATGA 1 cut(s) 1060
PfeI GAWTC 1 cut(s) 1036
PflFI GACNNNGTC 1 cut(s) 275
PleI GAGTC 3 cut(s) 161, 217, 1147
PpsI GAGTC 3 cut(s) 161, 217, 1147
Ppu21I YACGTR 1 cut(s) 945
PpuMI RGGWCCY 3 cut(s) 332, 346, 1096
Psp124BI GAGCTC 1 cut(s) 204
Psp5II RGGWCCY 3 cut(s) 332, 346, 1096
Psp6I CCWGG 5 cut(s) 285, 404, 641, 814, 854
PspEI GGTNACC 1 cut(s) 564
PspFI CCCAGC 3 cut(s) 466, 703, 1031
PspGI CCWGG 5 cut(s) 285, 404, 641, 814, 854
PspN4I GGNNCC 9 cut(s) 333, 334, 347, 403, 532, 640, 769, 813, 1098
PspPI GGNCC 6 cut(s) 289, 332, 346, 402, 639, 1096
PspPPI RGGWCCY 3 cut(s) 332, 346, 1096
PstI CTGCAG 1 cut(s) 844
PsuI RGATCY 1 cut(s) 550
PsyI GACNNNGTC 1 cut(s) 275
PvuII CAGCTG 1 cut(s) 845
RsaI GTAC 3 cut(s) 376, 613, 1228
RsaNI GTAC 3 cut(s) 375, 612, 1227
SacI GAGCTC 1 cut(s) 204
SaqAI TTAA 3 cut(s) 81, 459, 696
Sau3AI GATC 2 cut(s) 550, 1173
Sau96I GGNCC 6 cut(s) 289, 332, 346, 402, 639, 1096
SchI GAGTC 3 cut(s) 161, 218, 1148
ScrFI CCNGG 6 cut(s) 287, 343, 406, 643, 816, 856
SduI GDGCHC 2 cut(s) 204, 243
SfcI CTRYAG 1 cut(s) 840
SinI GGWCC 5 cut(s) 332, 346, 402, 639, 1096
Sse9I AATT 7 cut(s) 493, 730, 1050, 1115, 1164, 1221, 1250
SspMI CTAG 1 cut(s) 1136
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 6 cut(s) 285, 341, 404, 641, 814, 854
StyI CCWWGG 2 cut(s) 336, 794
TaiI ACGT 2 cut(s) 267, 947
TaqI TCGA 3 cut(s) 300, 959, 1188
TasI AATT 7 cut(s) 493, 730, 1050, 1115, 1164, 1221, 1250
TauI GCSGC 4 cut(s) 197, 234, 430, 667
TfiI GAWTC 1 cut(s) 1036
Tru1I TTAA 3 cut(s) 81, 459, 696
Tru9I TTAA 3 cut(s) 81, 459, 696
TscAI CASTG 1 cut(s) 1242
TseFI GTSAC 4 cut(s) 68, 112, 564, 781
TseI GCWGC 6 cut(s) 839, 842, 904, 1005, 1083, 1232
Tsp45I GTSAC 4 cut(s) 68, 112, 564, 781
TspDTI ATGAA 1 cut(s) 1034
TspGWI ACGGA 2 cut(s) 1166, 1252
TspRI CASTG 1 cut(s) 1242
Tth111I GACNNNGTC 1 cut(s) 275
VpaK11BI GGWCC 5 cut(s) 332, 346, 402, 639, 1096
XagI CCTNNNNNAGG 2 cut(s) 284, 1152
XcmI CCANNNNNNNNNTGG 1 cut(s) 862
XspI CTAG 1 cut(s) 1136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.