Rmu_ssc0000122.1_g000005

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000122.1
OG0021403
Physical Location & Seq
Reverse (-)
17194 .. 19460
2267 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 462 bp
atggcagctccgaaagtggagctcgaatctgattgtatcagatcaagctactgtctctctctctctgccatggctggtaccagattgactggccaatccacgacgttcacagacggtcgagctcagccaactggcggcggaggagtggctagtgacgaaggagatcgtcggcaacggaagctctggcgaatcgcgctgccgcttaatcaaatccgaaatcccgctgctgtggacttgatgaaagagggttcgactctaaccctgcgaaatgccgaaatttacatgttcaaaggaacaatgaggcttgctgtgggcgaaagtggcagtgttgaagtggccgagcctgccagttttaccgtgggggaagataacctctcactccttgattttaaacgtccacttcttggattgattaaatttttgggaattatgagatgtcttagggaaatgaaaggtctctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

16.6

Weight (kDa)

8.9

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 76 - 112 1.3e-11 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021403)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0443301
rosa_multiflora Rmu_sc0021666.1_g000002 Rmu_ssc0000122.1_g000005
rosa_samantha Rh4CG427300 Rh4DG407300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 77
AccB1I GGYRCC 1 cut(s) 77
AccB7I CCANNNNNTGG 1 cut(s) 404
AccII CGCG 1 cut(s) 194
AciI CCGC 4 cut(s) 135, 138, 200, 222
AcoI YGGCCR 2 cut(s) 91, 336
AcsI RAATTY 2 cut(s) 276, 416
AfaI GTAC 1 cut(s) 79
AfiI CCNNNNNNNGG 2 cut(s) 134, 404
AflIII ACRYGT 1 cut(s) 282
AgsI TTSAA 2 cut(s) 289, 332
AluBI AGCT 5 cut(s) 8, 22, 48, 122, 181
AluI AGCT 5 cut(s) 8, 22, 48, 122, 181
Alw21I GWGCWC 2 cut(s) 24, 124
Alw26I GTCTC 1 cut(s) 59
AoxI GGCC 2 cut(s) 91, 336
ApeKI GCWGC 3 cut(s) 5, 196, 224
ApoI RAATTY 2 cut(s) 276, 416
Asp718I GGTACC 1 cut(s) 77
AspLEI GCGC 1 cut(s) 196
BalI TGGCCA 1 cut(s) 93
BanI GGYRCC 1 cut(s) 77
BanII GRGCYC 2 cut(s) 24, 124
Bbv12I GWGCWC 2 cut(s) 24, 124
BbvI GCAGC 3 cut(s) 17, 183, 211
BcoDI GTCTC 1 cut(s) 59
BfaI CTAG 1 cut(s) 150
BisI GCNGC 5 cut(s) 6, 136, 197, 200, 225
BlpI GCTNAGC 1 cut(s) 123
BlsI GCNGC 5 cut(s) 7, 137, 198, 201, 226
BmiI GGNNCC 1 cut(s) 79
Bpu1102I GCTNAGC 1 cut(s) 123
BsaJI CCNNGG 2 cut(s) 69, 357
Bsc4I CCNNNNNNNGG 2 cut(s) 134, 404
Bse1I ACTGG 3 cut(s) 94, 136, 348
BseDI CCNNGG 2 cut(s) 69, 357
BseLI CCNNNNNNNGG 2 cut(s) 134, 404
BseMII CTCAG 1 cut(s) 137
BseNI ACTGG 3 cut(s) 94, 136, 348
BseRI GAGGAG 1 cut(s) 156
BseXI GCAGC 3 cut(s) 17, 183, 211
Bsh1236I CGCG 1 cut(s) 194
Bsh1285I CGRYCG 1 cut(s) 118
BshFI GGCC 2 cut(s) 93, 338
BshNI GGYRCC 1 cut(s) 77
BsiEI CGRYCG 1 cut(s) 118
BsiHKAI GWGCWC 2 cut(s) 24, 124
BslI CCNNNNNNNGG 2 cut(s) 134, 404
BsmAI GTCTC 1 cut(s) 59
BsnI GGCC 2 cut(s) 93, 338
Bsp1286I GDGCHC 2 cut(s) 24, 124
Bsp143I GATC 2 cut(s) 41, 163
Bsp1720I GCTNAGC 1 cut(s) 123
Bsp19I CCATGG 1 cut(s) 69
BspACI CCGC 4 cut(s) 135, 138, 200, 222
BspANI GGCC 2 cut(s) 93, 338
BspCNI CTCAG 1 cut(s) 136
BspFNI CGCG 1 cut(s) 194
BspLI GGNNCC 1 cut(s) 79
BspT107I GGYRCC 1 cut(s) 77
BsrI ACTGG 3 cut(s) 94, 136, 348
BssECI CCNNGG 2 cut(s) 69, 357
BssMI GATC 2 cut(s) 41, 163
BssT1I CCWWGG 1 cut(s) 69
Bst4CI ACNGT 3 cut(s) 53, 116, 358
BstC8I GCNNGC 2 cut(s) 306, 345
BstDEI CTNAG 2 cut(s) 123, 440
BstDSI CCRYGG 2 cut(s) 69, 357
BstFNI CGCG 1 cut(s) 194
BstHHI GCGC 1 cut(s) 196
BstKTI GATC 2 cut(s) 44, 166
BstMAI GTCTC 1 cut(s) 59
BstMBI GATC 2 cut(s) 41, 163
BstMCI CGRYCG 1 cut(s) 118
BstMWI GCNNNNNNNGC 4 cut(s) 178, 193, 321, 344
BstNSI RCATGY 1 cut(s) 286
BstUI CGCG 1 cut(s) 194
BstV1I GCAGC 3 cut(s) 17, 183, 211
BsuRI GGCC 2 cut(s) 93, 338
BtgI CCRYGG 2 cut(s) 69, 357
BtsI GCAGTG 1 cut(s) 331
BtsIMutI CAGTG 1 cut(s) 331
Cac8I GCNNGC 2 cut(s) 306, 345
CfoI GCGC 1 cut(s) 196
Csp6I GTAC 1 cut(s) 78
CviAII CATG 2 cut(s) 70, 283
CviQI GTAC 1 cut(s) 78
DdeI CTNAG 2 cut(s) 123, 440
DpnI GATC 2 cut(s) 43, 165
DpnII GATC 2 cut(s) 41, 163
DraI TTTAAA 1 cut(s) 391
EaeI YGGCCR 2 cut(s) 91, 336
EciI GGCGGA 1 cut(s) 153
Ecl136II GAGCTC 2 cut(s) 22, 122
Eco130I CCWWGG 1 cut(s) 69
Eco24I GRGCYC 2 cut(s) 24, 124
Eco53kI GAGCTC 2 cut(s) 22, 122
EcoICRI GAGCTC 2 cut(s) 22, 122
EcoT14I CCWWGG 1 cut(s) 69
EcoT38I GRGCYC 2 cut(s) 24, 124
ErhI CCWWGG 1 cut(s) 69
FaeI CATG 2 cut(s) 73, 286
FaiI YATR 3 cut(s) 71, 284, 431
FatI CATG 2 cut(s) 69, 282
FauI CCCGC 1 cut(s) 229
Fnu4HI GCNGC 5 cut(s) 6, 136, 197, 200, 225
FriOI GRGCYC 2 cut(s) 24, 124
Fsp4HI GCNGC 5 cut(s) 6, 136, 197, 200, 225
FspBI CTAG 1 cut(s) 150
GlaI GCGC 1 cut(s) 195
GluI GCNGC 5 cut(s) 6, 136, 197, 200, 225
HaeIII GGCC 2 cut(s) 93, 338
HhaI GCGC 1 cut(s) 196
Hin1II CATG 2 cut(s) 73, 286
Hin6I GCGC 1 cut(s) 194
HinP1I GCGC 1 cut(s) 194
HinfI GANTC 3 cut(s) 26, 189, 253
Hpy166II GTNNAC 3 cut(s) 108, 232, 398
Hpy188I TCNGA 4 cut(s) 12, 31, 41, 215
Hpy8I GTNNAC 3 cut(s) 108, 232, 398
Hpy99I CGWCG 2 cut(s) 106, 171
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 3 cut(s) 53, 116, 358
HpyCH4IV ACGT 2 cut(s) 104, 394
HpyF10VI GCNNNNNNNGC 4 cut(s) 178, 193, 321, 344
HpyF3I CTNAG 2 cut(s) 123, 440
HpySE526I ACGT 2 cut(s) 104, 394
Hsp92II CATG 2 cut(s) 73, 286
HspAI GCGC 1 cut(s) 194
KpnI GGTACC 1 cut(s) 81
Kzo9I GATC 2 cut(s) 41, 163
LmnI GCTCC 2 cut(s) 13, 19
LpnPI CCDG 8 cut(s) 60, 75, 94, 117, 169, 275, 357, 361
Lsp1109I GCAGC 3 cut(s) 17, 183, 211
MaeI CTAG 1 cut(s) 150
MaeII ACGT 2 cut(s) 104, 394
MaeIII GTNAC 1 cut(s) 152
MalI GATC 2 cut(s) 43, 165
MboI GATC 2 cut(s) 41, 163
MboII GAAGA 1 cut(s) 377
MhlI GDGCHC 2 cut(s) 24, 124
MlsI TGGCCA 1 cut(s) 93
MluCI AATT 3 cut(s) 276, 416, 426
MluNI TGGCCA 1 cut(s) 93
MlyI GAGTC 1 cut(s) 247
MnlI CCTC 4 cut(s) 134, 238, 294, 383
Mox20I TGGCCA 1 cut(s) 93
MscI TGGCCA 1 cut(s) 93
MseI TTAA 3 cut(s) 204, 390, 414
Msp20I TGGCCA 1 cut(s) 93
MspA1I CMGCKG 1 cut(s) 224
MvnI CGCG 1 cut(s) 194
MwoI GCNNNNNNNGC 4 cut(s) 178, 193, 321, 344
NcoI CCATGG 1 cut(s) 69
NdeII GATC 2 cut(s) 41, 163
NlaIII CATG 2 cut(s) 73, 286
NlaIV GGNNCC 1 cut(s) 79
NmeAIII GCCGAG 1 cut(s) 364
NmuCI GTSAC 1 cut(s) 152
NspI RCATGY 1 cut(s) 286
PciI ACATGT 1 cut(s) 282
PfeI GAWTC 2 cut(s) 26, 189
PflMI CCANNNNNTGG 1 cut(s) 404
PkrI GCNGC 5 cut(s) 7, 137, 198, 201, 226
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
PscI ACATGT 1 cut(s) 282
Psp124BI GAGCTC 2 cut(s) 24, 124
PspN4I GGNNCC 1 cut(s) 79
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
SacI GAGCTC 2 cut(s) 24, 124
SaqAI TTAA 3 cut(s) 204, 390, 414
SatI GCNGC 5 cut(s) 6, 136, 197, 200, 225
Sau3AI GATC 2 cut(s) 41, 163
SchI GAGTC 1 cut(s) 247
SduI GDGCHC 2 cut(s) 24, 124
SetI ASST 9 cut(s) 10, 24, 50, 107, 124, 183, 375, 397, 457
Sse9I AATT 3 cut(s) 276, 416, 426
SsiI CCGC 4 cut(s) 135, 138, 200, 222
SspMI CTAG 1 cut(s) 150
SstI GAGCTC 2 cut(s) 24, 124
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 3 cut(s) 53, 116, 358
TaiI ACGT 2 cut(s) 107, 397
TaqI TCGA 3 cut(s) 24, 118, 251
TasI AATT 3 cut(s) 276, 416, 426
TauI GCSGC 2 cut(s) 138, 202
TfiI GAWTC 2 cut(s) 26, 189
Tru1I TTAA 3 cut(s) 204, 390, 414
Tru9I TTAA 3 cut(s) 204, 390, 414
TscAI CASTG 1 cut(s) 331
TseFI GTSAC 1 cut(s) 152
TseI GCWGC 3 cut(s) 5, 196, 224
Tsp45I GTSAC 1 cut(s) 152
TspDTI ATGAA 1 cut(s) 254
TspGWI ACGGA 1 cut(s) 190
TspRI CASTG 1 cut(s) 331
Van91I CCANNNNNTGG 1 cut(s) 404
XapI RAATTY 2 cut(s) 276, 416
XceI RCATGY 1 cut(s) 286
XcmI CCANNNNNNNNNTGG 1 cut(s) 355
XspI CTAG 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.