Rmu_ssc0000124.1_g000008

of pyruvate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000124.1
OG0031006
Physical Location & Seq
Reverse (-)
32794 .. 39537
6744 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 528 bp
atggcgctctcacggtttcgacaccctgtgatctcacgcgccccttctctcttccgagctcgccttctctcctcgtccaccagatcactcactcgaagttccagtgtgcagaatttgactgtgggaggtgatagtgctcttttaaggcctgcatctttgtccatgctcaccggagtccagtataaattttcatacctcaagccttggacgggtgtcaagcattactcaactgccgatcctttgtatgctgtccttgatatgcctgctttgtctcctacaatgagtcaaggtaacattgctaaatggaggaagaaagagggagataaggccagtatccttgctgtaggcagaggcaacaaagttgttgaaccagtaattggaagtgatggaattgaaaggccagcagttgtcacgaaaatgaacttgacgttatcggcggatcatcgtgtttttgatggcaaagttggaggtacattcctctcagctttacaatcaaacttcagtgacatccgaagacttcttttgtaa

Protein Analysis

175

Amino Acids

19.03

Weight (kDa)

10.64

Isoelectric Point (pI)

57.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxoacid_dh PF00198 109 - 174 3.9e-07 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0031006)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0033311.1_g000001 Rmu_ssc0000124.1_g000008

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 377
AccII CGCG 1 cut(s) 39
AciI CCGC 1 cut(s) 437
AclWI GGATC 2 cut(s) 230, 447
AcsI RAATTY 2 cut(s) 112, 185
AcuI CTGAAG 1 cut(s) 484
AdeI CACNNNGTG 1 cut(s) 28
AfaI GTAC 1 cut(s) 472
AfiI CCNNNNNNNGG 2 cut(s) 209, 377
AgsI TTSAA 2 cut(s) 368, 395
AjuI GAANNNNNNNTTGG 2 cut(s) 360, 392
AluBI AGCT 2 cut(s) 59, 485
AluI AGCT 2 cut(s) 59, 485
Alw21I GWGCWC 2 cut(s) 61, 139
Alw26I GTCTC 1 cut(s) 276
AlwI GGATC 2 cut(s) 230, 447
AoxI GGCC 3 cut(s) 146, 327, 398
ApoI RAATTY 2 cut(s) 112, 185
AspLEI GCGC 2 cut(s) 7, 41
AsuHPI GGTGA 2 cut(s) 140, 160
BanII GRGCYC 1 cut(s) 61
BbsI GAAGAC 1 cut(s) 520
Bbv12I GWGCWC 2 cut(s) 61, 139
BccI CCATC 2 cut(s) 380, 449
BciVI GTATCC 1 cut(s) 344
BcoDI GTCTC 1 cut(s) 276
BfmI CTRYAG 1 cut(s) 342
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 1 cut(s) 344
BmsI GCATC 1 cut(s) 161
BoxI GACNNNNGTC 1 cut(s) 212
BpiI GAAGAC 1 cut(s) 520
BpuEI CTTGAG 1 cut(s) 182
BsaJI CCNNGG 1 cut(s) 203
BsaWI WCCGGW 1 cut(s) 170
Bsc4I CCNNNNNNNGG 2 cut(s) 209, 377
Bse1I ACTGG 4 cut(s) 102, 178, 330, 371
Bse3DI GCAATG 1 cut(s) 294
BseDI CCNNGG 1 cut(s) 203
BseGI GGATG 1 cut(s) 507
BseLI CCNNNNNNNGG 2 cut(s) 209, 377
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 1 cut(s) 495
BseNI ACTGG 4 cut(s) 102, 178, 330, 371
BseRI GAGGAG 1 cut(s) 61
BsgI GTGCAG 1 cut(s) 128
Bsh1236I CGCG 1 cut(s) 39
BshFI GGCC 3 cut(s) 148, 329, 400
BsiHKAI GWGCWC 2 cut(s) 61, 139
BsiSI CCGG 1 cut(s) 171
BslI CCNNNNNNNGG 2 cut(s) 209, 377
BsmAI GTCTC 1 cut(s) 276
BsnI GGCC 3 cut(s) 148, 329, 400
Bsp1286I GDGCHC 2 cut(s) 61, 139
Bsp143I GATC 4 cut(s) 30, 83, 235, 439
BspACI CCGC 1 cut(s) 437
BspANI GGCC 3 cut(s) 148, 329, 400
BspCNI CTCAG 1 cut(s) 494
BspFNI CGCG 1 cut(s) 39
BspPI GGATC 2 cut(s) 230, 447
BsrDI GCAATG 1 cut(s) 294
BsrI ACTGG 4 cut(s) 102, 178, 330, 371
BssECI CCNNGG 1 cut(s) 203
BssMI GATC 4 cut(s) 30, 83, 235, 439
BssT1I CCWWGG 1 cut(s) 203
Bst4CI ACNGT 2 cut(s) 15, 121
Bst6I CTCTTC 1 cut(s) 56
BstC8I GCNNGC 4 cut(s) 61, 150, 264, 402
BstDEI CTNAG 1 cut(s) 481
BstF5I GGATG 1 cut(s) 507
BstFNI CGCG 1 cut(s) 39
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 41
BstKTI GATC 4 cut(s) 33, 86, 238, 442
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 4 cut(s) 30, 83, 235, 439
BstPAI GACNNNNGTC 1 cut(s) 212
BstSFI CTRYAG 1 cut(s) 342
BstUI CGCG 1 cut(s) 39
BstV2I GAAGAC 1 cut(s) 520
BsuI GTATCC 1 cut(s) 344
BsuRI GGCC 3 cut(s) 148, 329, 400
BtsCI GGATG 1 cut(s) 507
BtsIMutI CAGTG 2 cut(s) 109, 508
Cac8I GCNNGC 4 cut(s) 61, 150, 264, 402
CfoI GCGC 2 cut(s) 7, 41
Csp6I GTAC 1 cut(s) 471
CviAII CATG 1 cut(s) 163
CviJI RGCY 6 cut(s) 59, 148, 202, 329, 400, 485
CviKI_1 RGCY 6 cut(s) 59, 148, 202, 329, 400, 485
CviQI GTAC 1 cut(s) 471
DdeI CTNAG 1 cut(s) 481
DpnI GATC 4 cut(s) 32, 85, 237, 441
DpnII GATC 4 cut(s) 30, 83, 235, 439
DraIII CACNNNGTG 1 cut(s) 28
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
EciI GGCGGA 1 cut(s) 452
Ecl136II GAGCTC 1 cut(s) 59
Eco130I CCWWGG 1 cut(s) 203
Eco147I AGGCCT 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 61
Eco53kI GAGCTC 1 cut(s) 59
Eco57I CTGAAG 1 cut(s) 484
EcoICRI GAGCTC 1 cut(s) 59
EcoT14I CCWWGG 1 cut(s) 203
EcoT38I GRGCYC 1 cut(s) 61
ErhI CCWWGG 1 cut(s) 203
FaeI CATG 1 cut(s) 166
FaiI YATR 5 cut(s) 164, 183, 193, 246, 260
FatI CATG 1 cut(s) 162
FokI GGATG 1 cut(s) 494
FriOI GRGCYC 1 cut(s) 61
GlaI GCGC 2 cut(s) 6, 40
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 3 cut(s) 148, 329, 400
HapII CCGG 1 cut(s) 171
HhaI GCGC 2 cut(s) 7, 41
Hin1II CATG 1 cut(s) 166
Hin6I GCGC 2 cut(s) 5, 39
HinP1I GCGC 2 cut(s) 5, 39
HinfI GANTC 2 cut(s) 174, 283
HpaII CCGG 1 cut(s) 171
HphI GGTGA 2 cut(s) 140, 160
Hpy166II GTNNAC 1 cut(s) 78
Hpy188I TCNGA 2 cut(s) 56, 512
Hpy188III TCNNGA 1 cut(s) 412
Hpy8I GTNNAC 1 cut(s) 78
HpyAV CCTTC 2 cut(s) 54, 74
HpyCH4III ACNGT 2 cut(s) 15, 121
HpyCH4IV ACGT 1 cut(s) 428
HpyCH4V TGCA 2 cut(s) 109, 152
HpyF3I CTNAG 1 cut(s) 481
HpySE526I ACGT 1 cut(s) 428
Hsp92II CATG 1 cut(s) 166
HspAI GCGC 2 cut(s) 5, 39
Kzo9I GATC 4 cut(s) 30, 83, 235, 439
LweI GCATC 1 cut(s) 161
MaeII ACGT 1 cut(s) 428
MaeIII GTNAC 3 cut(s) 290, 409, 503
MalI GATC 4 cut(s) 32, 85, 237, 441
MboI GATC 4 cut(s) 30, 83, 235, 439
MboII GAAGA 3 cut(s) 43, 322, 525
MhlI GDGCHC 2 cut(s) 61, 139
MluCI AATT 4 cut(s) 112, 185, 375, 390
MlyI GAGTC 2 cut(s) 183, 292
MmeI TCCRAC 1 cut(s) 445
MnlI CCTC 8 cut(s) 82, 119, 206, 300, 310, 344, 461, 488
MseI TTAA 1 cut(s) 143
MslI CAYNNNNRTG 1 cut(s) 416
MspI CCGG 1 cut(s) 171
MvnI CGCG 1 cut(s) 39
NdeII GATC 4 cut(s) 30, 83, 235, 439
NlaIII CATG 1 cut(s) 166
NmuCI GTSAC 2 cut(s) 409, 503
PceI AGGCCT 1 cut(s) 148
PflMI CCANNNNNTGG 1 cut(s) 377
PleI GAGTC 2 cut(s) 182, 291
PpsI GAGTC 2 cut(s) 182, 291
PshAI GACNNNNGTC 1 cut(s) 212
Psp124BI GAGCTC 1 cut(s) 61
RsaI GTAC 1 cut(s) 472
RsaNI GTAC 1 cut(s) 471
RseI CAYNNNNRTG 1 cut(s) 416
SacI GAGCTC 1 cut(s) 61
SaqAI TTAA 1 cut(s) 143
Sau3AI GATC 4 cut(s) 30, 83, 235, 439
SchI GAGTC 2 cut(s) 183, 292
SduI GDGCHC 2 cut(s) 61, 139
SetI ASST 7 cut(s) 61, 130, 198, 292, 431, 472, 487
SfaNI GCATC 1 cut(s) 161
SfcI CTRYAG 1 cut(s) 342
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
Sse9I AATT 4 cut(s) 112, 185, 375, 390
SseBI AGGCCT 1 cut(s) 148
SsiI CCGC 1 cut(s) 437
SstI GAGCTC 1 cut(s) 61
StuI AGGCCT 1 cut(s) 148
StyI CCWWGG 1 cut(s) 203
TaaI ACNGT 2 cut(s) 15, 121
TaiI ACGT 1 cut(s) 431
TaqI TCGA 2 cut(s) 19, 94
TasI AATT 4 cut(s) 112, 185, 375, 390
Tru1I TTAA 1 cut(s) 143
Tru9I TTAA 1 cut(s) 143
TscAI CASTG 2 cut(s) 109, 508
TseFI GTSAC 2 cut(s) 409, 503
Tsp45I GTSAC 2 cut(s) 409, 503
TspDTI ATGAA 2 cut(s) 180, 434
TspRI CASTG 2 cut(s) 109, 508
Van91I CCANNNNNTGG 1 cut(s) 377
XapI RAATTY 2 cut(s) 112, 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.