Rmu_ssc0000295.1_g000020

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000295.1
OG0023025
Physical Location & Seq
Reverse (-)
87068 .. 88218
1151 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 639 bp
atggggaaagccggagaagatgactacaagtctgcaatgaaatcagtacaggaagaagaactcgactcaatatcacgaagagaagaactggttgcatctcatcgaattgctgcgccgaggtggcgactaggcaaggacttgaagtgtgttgcgttggtgaggggcttaaggctgctgtggtggttacccaccctgggttctctgaatccggtgcttgaatctaggaattgggtcttcgttgggcccaagtcctatcatgttagttctactaccactgcttgccccagttataaggcagagtataaggcaaaggacaaatctaaaagattggaagttgaaagtgattcggccattgtggttaatctcataaattctggaaatactgatagtcaccatcttgggactcttattgccaattgcagagacatgatacaatattgtgatctctgttctatccaccatatccatagggaacagaactttgcagcggatattttggcaaaggataatatcaacttctctagaggaacaattttttgggatcaacctccccctcatacagttcagaccctccttgatgatgttgctaacagtggccatactagagttgtaggctctaacttcttgttccctaattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

212

Amino Acids

23.83

Weight (kDa)

7.11

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 107 - 169 9.1e-13 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023025)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002048.1_g000024 Rmu_ssc0000295.1_g000020
rosa_samantha Rh6CG110300 Rh6DG099900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 291
AciI CCGC 1 cut(s) 488
AclWI GGATC 1 cut(s) 549
AcoI YGGCCR 2 cut(s) 348, 595
AcsI RAATTY 1 cut(s) 370
AfaI GTAC 1 cut(s) 48
AfiI CCNNNNNNNGG 1 cut(s) 194
AflII CTTAAG 1 cut(s) 166
AgsI TTSAA 3 cut(s) 142, 218, 338
AhdI GACNNNNNGTC 1 cut(s) 28
AjnI CCWGG 1 cut(s) 192
AjuI GAANNNNNNNTTGG 2 cut(s) 520, 552
Alw26I GTCTC 1 cut(s) 417
AlwI GGATC 1 cut(s) 549
AoxI GGCC 3 cut(s) 242, 348, 595
ApaI GGGCCC 1 cut(s) 246
ApeKI GCWGC 3 cut(s) 110, 172, 485
ApoI RAATTY 1 cut(s) 370
AspLEI GCGC 1 cut(s) 115
AspS9I GGNCC 2 cut(s) 242, 243
AsuHPI GGTGA 2 cut(s) 169, 383
BaeGI GKGCMC 1 cut(s) 246
BalI TGGCCA 1 cut(s) 597
BanII GRGCYC 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 226
BbvI GCAGC 3 cut(s) 97, 159, 497
BccI CCATC 1 cut(s) 402
BciT130I CCWGG 1 cut(s) 194
BcoDI GTCTC 1 cut(s) 417
BfaI CTAG 4 cut(s) 128, 222, 522, 603
BfrI CTTAAG 1 cut(s) 166
BglI GCCNNNNNGGC 1 cut(s) 121
BisI GCNGC 3 cut(s) 111, 173, 486
BlsI GCNGC 3 cut(s) 112, 174, 487
Bme1390I CCNGG 1 cut(s) 194
BmeRI GACNNNNNGTC 1 cut(s) 28
BmgT120I GGNCC 2 cut(s) 242, 243
BmiI GGNNCC 1 cut(s) 244
BmrFI CCNGG 1 cut(s) 194
BmrI ACTGGG 1 cut(s) 279
BmsI GCATC 1 cut(s) 104
BmuI ACTGGG 1 cut(s) 279
BpiI GAAGAC 1 cut(s) 226
BsaJI CCNNGG 3 cut(s) 116, 192, 193
BsaWI WCCGGW 1 cut(s) 208
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse1I ACTGG 2 cut(s) 93, 285
Bse3DI GCAATG 1 cut(s) 42
BseBI CCWGG 1 cut(s) 194
BseDI CCNNGG 3 cut(s) 116, 192, 193
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMI GCAATG 1 cut(s) 42
BseNI ACTGG 2 cut(s) 93, 285
BseSI GKGCMC 1 cut(s) 246
BseXI GCAGC 3 cut(s) 97, 159, 497
BshFI GGCC 3 cut(s) 244, 350, 597
BsiSI CCGG 2 cut(s) 12, 209
BslFI GGGAC 1 cut(s) 415
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 1 cut(s) 417
BsmFI GGGAC 1 cut(s) 415
BsnI GGCC 3 cut(s) 244, 350, 597
Bsp120I GGGCCC 1 cut(s) 242
Bsp1286I GDGCHC 1 cut(s) 246
Bsp143I GATC 2 cut(s) 442, 541
BspACI CCGC 1 cut(s) 488
BspANI GGCC 3 cut(s) 244, 350, 597
BspLI GGNNCC 1 cut(s) 244
BspPI GGATC 1 cut(s) 549
BspTI CTTAAG 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 42
BsrI ACTGG 2 cut(s) 93, 285
BssECI CCNNGG 3 cut(s) 116, 192, 193
BssMI GATC 2 cut(s) 442, 541
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 2 cut(s) 562, 593
Bst6I CTCTTC 1 cut(s) 73
BstAFI CTTAAG 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 280
BstEII GGTNACC 1 cut(s) 183
BstHHI GCGC 1 cut(s) 115
BstKTI GATC 2 cut(s) 445, 544
BstMAI GTCTC 1 cut(s) 417
BstMBI GATC 2 cut(s) 442, 541
BstMWI GCNNNNNNNGC 1 cut(s) 121
BstNI CCWGG 1 cut(s) 194
BstPI GGTNACC 1 cut(s) 183
BstSCI CCNGG 1 cut(s) 192
BstSLI GKGCMC 1 cut(s) 246
BstV1I GCAGC 3 cut(s) 97, 159, 497
BstV2I GAAGAC 1 cut(s) 226
BsuRI GGCC 3 cut(s) 244, 350, 597
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 2 cut(s) 273, 598
Cac8I GCNNGC 1 cut(s) 280
CfoI GCGC 1 cut(s) 115
Cfr13I GGNCC 2 cut(s) 242, 243
Csp6I GTAC 1 cut(s) 47
CviAII CATG 2 cut(s) 257, 427
CviJI RGCY 7 cut(s) 11, 165, 172, 244, 350, 597, 615
CviKI_1 RGCY 7 cut(s) 11, 165, 172, 244, 350, 597, 615
CviQI GTAC 1 cut(s) 47
DpnI GATC 2 cut(s) 444, 543
DpnII GATC 2 cut(s) 442, 541
DriI GACNNNNNGTC 1 cut(s) 28
EaeI YGGCCR 2 cut(s) 348, 595
Eam1104I CTCTTC 1 cut(s) 73
Eam1105I GACNNNNNGTC 1 cut(s) 28
EarI CTCTTC 1 cut(s) 73
Eco24I GRGCYC 1 cut(s) 246
Eco91I GGTNACC 1 cut(s) 183
EcoO65I GGTNACC 1 cut(s) 183
EcoRII CCWGG 1 cut(s) 192
EcoT38I GRGCYC 1 cut(s) 246
FaeI CATG 2 cut(s) 260, 430
FaiI YATR 9 cut(s) 258, 291, 303, 368, 428, 462, 468, 558, 600
FaqI GGGAC 1 cut(s) 415
FatI CATG 2 cut(s) 256, 426
Fnu4HI GCNGC 3 cut(s) 111, 173, 486
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 3 cut(s) 111, 173, 486
FspBI CTAG 4 cut(s) 128, 222, 522, 603
GlaI GCGC 1 cut(s) 114
GluI GCNGC 3 cut(s) 111, 173, 486
HaeIII GGCC 3 cut(s) 244, 350, 597
HapII CCGG 2 cut(s) 12, 209
HhaI GCGC 1 cut(s) 115
Hin1II CATG 2 cut(s) 260, 430
Hin6I GCGC 1 cut(s) 113
HinP1I GCGC 1 cut(s) 113
HinfI GANTC 5 cut(s) 65, 205, 218, 344, 403
HpaII CCGG 2 cut(s) 12, 209
HphI GGTGA 2 cut(s) 169, 383
Hpy188I TCNGA 2 cut(s) 204, 567
Hpy188III TCNNGA 3 cut(s) 75, 375, 522
HpyCH4III ACNGT 2 cut(s) 562, 593
HpyCH4V TGCA 4 cut(s) 35, 95, 420, 485
HpyF10VI GCNNNNNNNGC 1 cut(s) 121
Hsp92II CATG 2 cut(s) 260, 430
HspAI GCGC 1 cut(s) 113
Kzo9I GATC 2 cut(s) 442, 541
LpnPI CCDG 8 cut(s) 25, 35, 74, 179, 206, 222, 298, 360
Lsp1109I GCAGC 3 cut(s) 97, 159, 497
LweI GCATC 1 cut(s) 104
MaeI CTAG 4 cut(s) 128, 222, 522, 603
MaeIII GTNAC 2 cut(s) 183, 389
MalI GATC 2 cut(s) 444, 543
MboI GATC 2 cut(s) 442, 541
MboII GAAGA 6 cut(s) 29, 65, 68, 90, 95, 226
MfeI CAATTG 1 cut(s) 415
MhlI GDGCHC 1 cut(s) 246
MlsI TGGCCA 1 cut(s) 597
MluCI AATT 6 cut(s) 105, 226, 370, 415, 531, 634
MluNI TGGCCA 1 cut(s) 597
MlyI GAGTC 2 cut(s) 59, 397
MnlI CCTC 6 cut(s) 111, 153, 518, 558, 564, 581
Mox20I TGGCCA 1 cut(s) 597
MscI TGGCCA 1 cut(s) 597
MseI TTAA 2 cut(s) 167, 360
Msp20I TGGCCA 1 cut(s) 597
MspA1I CMGCKG 1 cut(s) 488
MspCI CTTAAG 1 cut(s) 166
MspI CCGG 2 cut(s) 12, 209
MspR9I CCNGG 1 cut(s) 194
MunI CAATTG 1 cut(s) 415
MvaI CCWGG 1 cut(s) 194
MwoI GCNNNNNNNGC 1 cut(s) 121
NdeII GATC 2 cut(s) 442, 541
NlaIII CATG 2 cut(s) 260, 430
NlaIV GGNNCC 1 cut(s) 244
NmeAIII GCCGAG 1 cut(s) 141
NmuCI GTSAC 1 cut(s) 389
PasI CCCWGGG 1 cut(s) 193
PfeI GAWTC 3 cut(s) 205, 218, 344
PkrI GCNGC 3 cut(s) 112, 174, 487
PleI GAGTC 2 cut(s) 59, 397
PpsI GAGTC 2 cut(s) 59, 397
PsiI TTATAA 1 cut(s) 291
Psp6I CCWGG 1 cut(s) 192
PspEI GGTNACC 1 cut(s) 183
PspGI CCWGG 1 cut(s) 192
PspN4I GGNNCC 1 cut(s) 244
PspOMI GGGCCC 1 cut(s) 242
PspPI GGNCC 2 cut(s) 242, 243
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
SaqAI TTAA 2 cut(s) 167, 360
SatI GCNGC 3 cut(s) 111, 173, 486
Sau3AI GATC 2 cut(s) 442, 541
Sau96I GGNCC 2 cut(s) 242, 243
SchI GAGTC 2 cut(s) 59, 397
ScrFI CCNGG 1 cut(s) 194
SduI GDGCHC 1 cut(s) 246
SetI ASST 2 cut(s) 122, 550
SfaNI GCATC 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
Sse9I AATT 6 cut(s) 105, 226, 370, 415, 531, 634
SsiI CCGC 1 cut(s) 488
SspI AATATT 1 cut(s) 437
SspMI CTAG 4 cut(s) 128, 222, 522, 603
StyD4I CCNGG 1 cut(s) 192
TaaI ACNGT 2 cut(s) 562, 593
TaqI TCGA 2 cut(s) 63, 103
TasI AATT 6 cut(s) 105, 226, 370, 415, 531, 634
TatI WGTACW 1 cut(s) 46
TfiI GAWTC 3 cut(s) 205, 218, 344
Tru1I TTAA 2 cut(s) 167, 360
Tru9I TTAA 2 cut(s) 167, 360
TscAI CASTG 2 cut(s) 280, 598
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 3 cut(s) 110, 172, 485
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 1 cut(s) 53
TspRI CASTG 2 cut(s) 280, 598
Vha464I CTTAAG 1 cut(s) 166
XapI RAATTY 1 cut(s) 370
XbaI TCTAGA 1 cut(s) 521
XspI CTAG 4 cut(s) 128, 222, 522, 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.