Rmu_ssc0000372.1_g000081

At5g08430

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000372.1
OG0030741
Physical Location & Seq
Reverse (-)
294292 .. 295205
914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000372.1_g000081.1.cds

Sequence Viewer

Length: 408 bp
atggggatggttgagttggctaatcgggaaacacatattcgttctatgtgtggtttgtttgatggtcaatcgccaacgacaactgagacttatggcagatggcggcgcagtggggcaattactcagtggcaaataaacatcgccgatgtcgacgccgctaccgctccgaggaggaggagcaagaggcttcactgccccaagaaatttgagtttctcaaatggggcttgaagctgctgattgaatttctcgagtcgattggcaaggacaggacgaagcagatctccgaatatgacgttactacgattatcatcgacaacgtcaaccactgcaggcttctccagccgacgaagaagaagaggatcgtctgcgaccaagggctgttaaaagcaagagagaattgtaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF Plus-3 SWIB

Protein Analysis

135

Amino Acids

15.62

Weight (kDa)

9.63

Isoelectric Point (pI)

64.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0030741)

Species Orthologous Gene IDs
rosa_laevigata RLG00000011574
rosa_multiflora Rmu_ssc0000372.1_g000081

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 164
AccI GTMKAC 1 cut(s) 150
AciI CCGC 3 cut(s) 103, 156, 162
AclWI GGATC 1 cut(s) 368
AcsI RAATTY 2 cut(s) 203, 242
AcyI GRCGYC 1 cut(s) 153
AfiI CCNNNNNNNGG 1 cut(s) 168
AgsI TTSAA 2 cut(s) 229, 242
AluBI AGCT 1 cut(s) 232
AluI AGCT 1 cut(s) 232
Alw26I GTCTC 1 cut(s) 80
AlwI GGATC 1 cut(s) 368
Ama87I CYCGRG 1 cut(s) 248
ApeKI GCWGC 1 cut(s) 232
ApoI RAATTY 2 cut(s) 203, 242
AspLEI GCGC 1 cut(s) 108
AvaI CYCGRG 1 cut(s) 248
BbvI GCAGC 1 cut(s) 219
BccI CCATC 2 cut(s) 56, 93
BcoDI GTCTC 1 cut(s) 80
BfmI CTRYAG 1 cut(s) 328
BglII AGATCT 1 cut(s) 279
BisI GCNGC 3 cut(s) 104, 156, 233
BlsI GCNGC 3 cut(s) 105, 157, 234
BmeT110I CYCGRG 1 cut(s) 248
BpmI CTGGAG 1 cut(s) 323
BsaBI GATNNNNATC 1 cut(s) 308
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 2 cut(s) 167, 373
Bsc4I CCNNNNNNNGG 1 cut(s) 168
Bse8I GATNNNNATC 1 cut(s) 308
BseDI CCNNGG 2 cut(s) 167, 373
BseGI GGATG 1 cut(s) 12
BseJI GATNNNNATC 1 cut(s) 308
BseLI CCNNNNNNNGG 1 cut(s) 168
BseMII CTCAG 2 cut(s) 75, 137
BseRI GAGGAG 3 cut(s) 184, 187, 190
BseXI GCAGC 1 cut(s) 219
BsiHKCI CYCGRG 1 cut(s) 248
BslI CCNNNNNNNGG 1 cut(s) 168
BsmAI GTCTC 1 cut(s) 80
BsoBI CYCGRG 1 cut(s) 248
Bsp143I GATC 2 cut(s) 279, 360
BspACI CCGC 3 cut(s) 103, 156, 162
BspCNI CTCAG 2 cut(s) 76, 136
BspMAI CTGCAG 1 cut(s) 332
BspPI GGATC 1 cut(s) 368
BsrBI CCGCTC 1 cut(s) 164
BssECI CCNNGG 2 cut(s) 167, 373
BssMI GATC 2 cut(s) 279, 360
BssNI GRCGYC 1 cut(s) 153
BssT1I CCWWGG 1 cut(s) 373
Bst6I CTCTTC 1 cut(s) 350
BstACI GRCGYC 1 cut(s) 153
BstC8I GCNNGC 1 cut(s) 332
BstDEI CTNAG 2 cut(s) 84, 123
BstF5I GGATG 1 cut(s) 12
BstHHI GCGC 1 cut(s) 108
BstKTI GATC 2 cut(s) 282, 363
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 2 cut(s) 279, 360
BstMWI GCNNNNNNNGC 2 cut(s) 161, 340
BstSFI CTRYAG 1 cut(s) 328
BstV1I GCAGC 1 cut(s) 219
BstX2I RGATCY 1 cut(s) 279
BstYI RGATCY 1 cut(s) 279
BtgZI GCGATG 1 cut(s) 124
BtsCI GGATG 1 cut(s) 12
BtsI GCAGTG 3 cut(s) 115, 190, 325
BtsIMutI CAGTG 4 cut(s) 115, 131, 190, 325
Cac8I GCNNGC 1 cut(s) 332
CfoI GCGC 1 cut(s) 108
CseI GACGC 1 cut(s) 161
CviJI RGCY 7 cut(s) 20, 187, 225, 232, 334, 343, 379
CviKI_1 RGCY 7 cut(s) 20, 187, 225, 232, 334, 343, 379
DdeI CTNAG 2 cut(s) 84, 123
DpnI GATC 2 cut(s) 281, 362
DpnII GATC 2 cut(s) 279, 360
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
Eco130I CCWWGG 1 cut(s) 373
Eco88I CYCGRG 1 cut(s) 248
EcoT14I CCWWGG 1 cut(s) 373
ErhI CCWWGG 1 cut(s) 373
FaiI YATR 4 cut(s) 36, 47, 93, 291
FblI GTMKAC 1 cut(s) 150
Fnu4HI GCNGC 3 cut(s) 104, 156, 233
FokI GGATG 1 cut(s) 19
Fsp4HI GCNGC 3 cut(s) 104, 156, 233
GlaI GCGC 1 cut(s) 107
GluI GCNGC 3 cut(s) 104, 156, 233
GsuI CTGGAG 1 cut(s) 323
HgaI GACGC 1 cut(s) 161
HhaI GCGC 1 cut(s) 108
Hin1I GRCGYC 1 cut(s) 153
Hin6I GCGC 1 cut(s) 106
HinP1I GCGC 1 cut(s) 106
HincII GTYRAC 2 cut(s) 151, 322
HindII GTYRAC 2 cut(s) 151, 322
HinfI GANTC 1 cut(s) 251
Hpy166II GTNNAC 2 cut(s) 151, 322
Hpy188I TCNGA 2 cut(s) 168, 286
Hpy188III TCNNGA 2 cut(s) 26, 248
Hpy8I GTNNAC 2 cut(s) 151, 322
Hpy99I CGWCG 2 cut(s) 155, 349
HpyCH4IV ACGT 2 cut(s) 294, 318
HpyCH4V TGCA 1 cut(s) 330
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 340
HpyF3I CTNAG 2 cut(s) 84, 123
HpySE526I ACGT 2 cut(s) 294, 318
Hsp92I GRCGYC 1 cut(s) 153
HspAI GCGC 1 cut(s) 106
Kzo9I GATC 2 cut(s) 279, 360
LmnI GCTCC 2 cut(s) 169, 177
LpnPI CCDG 3 cut(s) 253, 316, 353
Lsp1109I GCAGC 1 cut(s) 219
MaeII ACGT 2 cut(s) 294, 318
MaeIII GTNAC 2 cut(s) 295, 401
MalI GATC 2 cut(s) 281, 362
MbiI CCGCTC 1 cut(s) 164
MboI GATC 2 cut(s) 279, 360
MboII GAAGA 3 cut(s) 361, 364, 367
MflI RGATCY 1 cut(s) 279
MluCI AATT 4 cut(s) 117, 203, 242, 397
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 5 cut(s) 162, 165, 168, 177, 351
MseI TTAA 1 cut(s) 383
MwoI GCNNNNNNNGC 2 cut(s) 161, 340
NdeII GATC 2 cut(s) 279, 360
PaeR7I CTCGAG 1 cut(s) 248
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PflFI GACNNNGTC 1 cut(s) 317
PkrI GCNGC 3 cut(s) 105, 157, 234
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
PstI CTGCAG 1 cut(s) 332
PsuI RGATCY 1 cut(s) 279
PsyI GACNNNGTC 1 cut(s) 317
SalI GTCGAC 1 cut(s) 149
SaqAI TTAA 1 cut(s) 383
SatI GCNGC 3 cut(s) 104, 156, 233
Sau3AI GATC 2 cut(s) 279, 360
SchI GAGTC 1 cut(s) 260
SetI ASST 3 cut(s) 234, 297, 321
SfcI CTRYAG 1 cut(s) 328
Sfr274I CTCGAG 1 cut(s) 248
SlaI CTCGAG 1 cut(s) 248
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 4 cut(s) 117, 203, 242, 397
SsiI CCGC 3 cut(s) 103, 156, 162
StyI CCWWGG 1 cut(s) 373
TaiI ACGT 2 cut(s) 297, 321
TaqI TCGA 4 cut(s) 150, 249, 254, 312
TasI AATT 4 cut(s) 117, 203, 242, 397
TauI GCSGC 2 cut(s) 106, 158
Tru1I TTAA 1 cut(s) 383
Tru9I TTAA 1 cut(s) 383
TscAI CASTG 4 cut(s) 115, 131, 197, 332
TseI GCWGC 1 cut(s) 232
TspRI CASTG 4 cut(s) 115, 131, 197, 332
Tth111I GACNNNGTC 1 cut(s) 317
XapI RAATTY 2 cut(s) 203, 242
XhoI CTCGAG 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.