Rroxscaffold_171G00441420

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000171
OG0025099
Physical Location & Seq
Forward (+)
63955 .. 64320
366 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_171G00441420.1

Sequence Viewer

Length: 366 bp
ATGGACGTAGAGAGAGTTCAAACCTTGGCCTTGCGTGGGCTCAACGAGCTCCCAGCGAAGTTCATCCGCCCGACTCATGAGCAACCCGAGAACAGCAAGGCCTTGGAGGGAGTCATCGTGCCGGTGATCTCCCTAGCTAAACCACACGACATTGTCGTGAAGGAAGTGGCTGAGGCAGCCTTTGAGTGGGGCTTCTTTCTCATCACTGACCATGGCATACCGGCTTCTTTGATAGAGCAGTTACAGAAGGTGGGCAAAGAGTTCTCTACGCTGCCACAGGAGGAAAAAAAGGCTTATAAAAATGACCCTGCTAATGGCAAATTTGATGGGTACGGTTTAGTGGATTTAGTGGACCAAGATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko01100 ko01110 map00941 map01100 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

121

Amino Acids

13.45

Weight (kDa)

4.82

Isoelectric Point (pI)

30.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 40 - 112 4.1e-18 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025099)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0295251
rosa_roxburghii Rroxscaffold_171G00441420
rosa_samantha Rh6CG377200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 297
AciI CCGC 1 cut(s) 67
AcsI RAATTY 1 cut(s) 320
AfaI GTAC 1 cut(s) 332
AfiI CCNNNNNNNGG 3 cut(s) 36, 186, 314
AgsI TTSAA 1 cut(s) 20
AluBI AGCT 2 cut(s) 49, 137
AluI AGCT 2 cut(s) 49, 137
Alw21I GWGCWC 1 cut(s) 51
Ama87I CYCGRG 1 cut(s) 86
AoxI GGCC 2 cut(s) 27, 99
ApeKI GCWGC 2 cut(s) 176, 271
ApoI RAATTY 1 cut(s) 320
AspS9I GGNCC 1 cut(s) 352
AsuHPI GGTGA 1 cut(s) 136
AvaI CYCGRG 1 cut(s) 86
AvaII GGWCC 1 cut(s) 352
BanII GRGCYC 2 cut(s) 42, 51
Bbv12I GWGCWC 1 cut(s) 51
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 2 cut(s) 188, 258
BccI CCATC 1 cut(s) 320
BfaI CTAG 1 cut(s) 134
BisI GCNGC 2 cut(s) 177, 272
BlsI GCNGC 2 cut(s) 178, 273
Bme18I GGWCC 1 cut(s) 352
BmeT110I CYCGRG 1 cut(s) 86
BmgT120I GGNCC 1 cut(s) 352
Bpu10I CCTNAGC 1 cut(s) 171
BsaJI CCNNGG 3 cut(s) 24, 102, 211
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 186, 314
Bse118I RCCGGY 2 cut(s) 121, 220
BseDI CCNNGG 3 cut(s) 24, 102, 211
BseGI GGATG 1 cut(s) 63
BseLI CCNNNNNNNGG 3 cut(s) 36, 186, 314
BseMII CTCAG 1 cut(s) 162
BseXI GCAGC 2 cut(s) 188, 258
BseYI CCCAGC 1 cut(s) 52
BshFI GGCC 2 cut(s) 29, 101
BsiHKAI GWGCWC 1 cut(s) 51
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 2 cut(s) 122, 221
BslI CCNNNNNNNGG 3 cut(s) 36, 186, 314
BsnI GGCC 2 cut(s) 29, 101
BsoBI CYCGRG 1 cut(s) 86
Bsp1286I GDGCHC 2 cut(s) 42, 51
Bsp143I GATC 1 cut(s) 126
Bsp19I CCATGG 1 cut(s) 211
BspACI CCGC 1 cut(s) 67
BspANI GGCC 2 cut(s) 29, 101
BspCNI CTCAG 1 cut(s) 163
BspHI TCATGA 1 cut(s) 76
BsrFI RCCGGY 2 cut(s) 121, 220
BssAI RCCGGY 2 cut(s) 121, 220
BssECI CCNNGG 3 cut(s) 24, 102, 211
BssMI GATC 1 cut(s) 126
BssT1I CCWWGG 3 cut(s) 24, 102, 211
Bst4CI ACNGT 1 cut(s) 335
BstDEI CTNAG 1 cut(s) 171
BstDSI CCRYGG 1 cut(s) 211
BstF5I GGATG 1 cut(s) 63
BstKTI GATC 1 cut(s) 129
BstMBI GATC 1 cut(s) 126
BstMWI GCNNNNNNNGC 2 cut(s) 46, 176
BstV1I GCAGC 2 cut(s) 188, 258
BsuRI GGCC 2 cut(s) 29, 101
BtgI CCRYGG 1 cut(s) 211
BtsCI GGATG 1 cut(s) 63
BtsIMutI CAGTG 1 cut(s) 204
CciI TCATGA 1 cut(s) 76
Cfr10I RCCGGY 2 cut(s) 121, 220
Cfr13I GGNCC 1 cut(s) 352
Csp6I GTAC 1 cut(s) 331
CviAII CATG 2 cut(s) 77, 212
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 1 cut(s) 171
DpnI GATC 1 cut(s) 128
DpnII GATC 1 cut(s) 126
EciI GGCGGA 1 cut(s) 56
Ecl136II GAGCTC 1 cut(s) 49
Eco130I CCWWGG 3 cut(s) 24, 102, 211
Eco147I AGGCCT 1 cut(s) 101
Eco24I GRGCYC 2 cut(s) 42, 51
Eco47I GGWCC 1 cut(s) 352
Eco53kI GAGCTC 1 cut(s) 49
Eco88I CYCGRG 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 49
EcoT14I CCWWGG 3 cut(s) 24, 102, 211
EcoT38I GRGCYC 2 cut(s) 42, 51
ErhI CCWWGG 3 cut(s) 24, 102, 211
FaeI CATG 2 cut(s) 80, 215
FaiI YATR 4 cut(s) 78, 213, 218, 297
FatI CATG 2 cut(s) 76, 211
Fnu4HI GCNGC 2 cut(s) 177, 272
FokI GGATG 1 cut(s) 50
FriOI GRGCYC 2 cut(s) 42, 51
Fsp4HI GCNGC 2 cut(s) 177, 272
FspBI CTAG 1 cut(s) 134
GluI GCNGC 2 cut(s) 177, 272
GsaI CCCAGC 1 cut(s) 56
HaeIII GGCC 2 cut(s) 29, 101
HapII CCGG 2 cut(s) 122, 221
Hin1II CATG 2 cut(s) 80, 215
HinfI GANTC 2 cut(s) 73, 111
HpaII CCGG 2 cut(s) 122, 221
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 1 cut(s) 352
Hpy188III TCNNGA 2 cut(s) 77, 157
Hpy8I GTNNAC 1 cut(s) 352
HpyAV CCTTC 2 cut(s) 154, 241
HpyCH4III ACNGT 1 cut(s) 335
HpyCH4IV ACGT 1 cut(s) 6
HpyF10VI GCNNNNNNNGC 2 cut(s) 46, 176
HpyF3I CTNAG 1 cut(s) 171
HpySE526I ACGT 1 cut(s) 6
Hsp92II CATG 2 cut(s) 80, 215
Kzo9I GATC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 54
LpnPI CCDG 5 cut(s) 66, 135, 234, 263, 321
Lsp1109I GCAGC 2 cut(s) 188, 258
MaeI CTAG 1 cut(s) 134
MaeII ACGT 1 cut(s) 6
MaeIII GTNAC 1 cut(s) 240
MalI GATC 1 cut(s) 128
MboI GATC 1 cut(s) 126
MhlI GDGCHC 2 cut(s) 42, 51
MluCI AATT 1 cut(s) 320
MlyI GAGTC 2 cut(s) 67, 120
MnlI CCTC 3 cut(s) 100, 166, 274
MslI CAYNNNNRTG 1 cut(s) 155
MspI CCGG 2 cut(s) 122, 221
MwoI GCNNNNNNNGC 2 cut(s) 46, 176
NcoI CCATGG 1 cut(s) 211
NdeII GATC 1 cut(s) 126
NlaIII CATG 2 cut(s) 80, 215
PagI TCATGA 1 cut(s) 76
PceI AGGCCT 1 cut(s) 101
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PflFI GACNNNGTC 1 cut(s) 152
PkrI GCNGC 2 cut(s) 178, 273
PleI GAGTC 2 cut(s) 67, 119
PpsI GAGTC 2 cut(s) 67, 119
PsiI TTATAA 1 cut(s) 297
Psp124BI GAGCTC 1 cut(s) 51
PspFI CCCAGC 1 cut(s) 52
PspPI GGNCC 1 cut(s) 352
PsrI GAACNNNNNNTAC 1 cut(s) 32
PsyI GACNNNGTC 1 cut(s) 152
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
RseI CAYNNNNRTG 1 cut(s) 155
SacI GAGCTC 1 cut(s) 51
SatI GCNGC 2 cut(s) 177, 272
Sau3AI GATC 1 cut(s) 126
Sau96I GGNCC 1 cut(s) 352
SchI GAGTC 2 cut(s) 67, 120
SduI GDGCHC 2 cut(s) 42, 51
SetI ASST 5 cut(s) 9, 26, 51, 139, 252
SinI GGWCC 1 cut(s) 352
SmiMI CAYNNNNRTG 1 cut(s) 155
Sse9I AATT 1 cut(s) 320
SseBI AGGCCT 1 cut(s) 101
SsiI CCGC 1 cut(s) 67
SspMI CTAG 1 cut(s) 134
SstI GAGCTC 1 cut(s) 51
StuI AGGCCT 1 cut(s) 101
StyI CCWWGG 3 cut(s) 24, 102, 211
TaaI ACNGT 1 cut(s) 335
TaiI ACGT 1 cut(s) 9
TasI AATT 1 cut(s) 320
TscAI CASTG 1 cut(s) 211
TseI GCWGC 2 cut(s) 176, 271
TspDTI ATGAA 1 cut(s) 52
TspRI CASTG 1 cut(s) 211
Tth111I GACNNNGTC 1 cut(s) 152
VpaK11BI GGWCC 1 cut(s) 352
XapI RAATTY 1 cut(s) 320
XspI CTAG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.