Rh6CG377200

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0025099
Physical Location & Seq
Forward (+)
56430104 .. 56430526
423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG377200.1

Sequence Viewer

Length: 423 bp
ATGCAGGTAGAGAGAGTTCAGGCGTTGGCCCTCGGCATGCTCAACGAGTTACCGGAAAAGTTCATCCGCCCAGCCCACGAGCAGCCCTTGAACACCAAGGCCGTGGAGGAAATCGCGGTGCCGGTCATTTCCCTAGCAAAGCCACACAACATCATCGTCGAGGAAGCTTCCGAGGCAGCTGCCCAATGGGGCTTCTTTCTGATCACTGACCATGGGATACCAGCTTCATTGATAGAGGAGTTACAGAAGGTTGGCAAGGAGTTTTATATGCTGCCACAGGAGGAAAAAGAGGCCTATGCAAATGACCCTGCTAGTGGCAGATTTGATGGGTATGGAACTAGGATGACCAAGAACCATGATGACAAGGTTGAGTGGATTGACTATTTCTTTCACCACACTGCTCCCCCATCTAAGGTTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko01100 ko01110 map00941 map01100 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

140

Amino Acids

15.83

Weight (kDa)

5.07

Isoelectric Point (pI)

49.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 40 - 137 3.4e-22 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0025099)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0295251
rosa_roxburghii Rroxscaffold_171G00441420
rosa_samantha Rh6CG377200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 118
AccII CGCG 1 cut(s) 116
AciI CCGC 2 cut(s) 67, 116
AfiI CCNNNNNNNGG 2 cut(s) 314, 412
AgsI TTSAA 1 cut(s) 91
AluBI AGCT 3 cut(s) 167, 179, 224
AluI AGCT 3 cut(s) 167, 179, 224
AoxI GGCC 3 cut(s) 27, 99, 291
ApeKI GCWGC 4 cut(s) 82, 176, 179, 271
AspS9I GGNCC 1 cut(s) 28
AsuHPI GGTGA 1 cut(s) 383
BanI GGYRCC 1 cut(s) 118
BauI CACGAG 1 cut(s) 77
BbvI GCAGC 4 cut(s) 94, 166, 188, 258
BccI CCATC 2 cut(s) 320, 415
BceAI ACGGC 1 cut(s) 86
BcgI CGANNNNNNTGC 2 cut(s) 161, 195
BciVI GTATCC 1 cut(s) 210
BclI TGATCA 1 cut(s) 201
BfaI CTAG 4 cut(s) 134, 312, 339, 421
BfuI GTATCC 1 cut(s) 210
BisI GCNGC 4 cut(s) 83, 177, 180, 272
BlsI GCNGC 4 cut(s) 84, 178, 181, 273
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 1 cut(s) 120
BsaJI CCNNGG 5 cut(s) 31, 96, 102, 171, 211
BsaWI WCCGGW 1 cut(s) 52
Bsc4I CCNNNNNNNGG 2 cut(s) 314, 412
Bse118I RCCGGY 1 cut(s) 121
BseDI CCNNGG 5 cut(s) 31, 96, 102, 171, 211
BseGI GGATG 2 cut(s) 63, 348
BseLI CCNNNNNNNGG 2 cut(s) 314, 412
BseRI GAGGAG 1 cut(s) 251
BseXI GCAGC 4 cut(s) 94, 166, 188, 258
BseYI CCCAGC 1 cut(s) 70
Bsh1236I CGCG 1 cut(s) 116
BshFI GGCC 3 cut(s) 29, 101, 293
BshNI GGYRCC 1 cut(s) 118
BsiSI CCGG 2 cut(s) 53, 122
BslI CCNNNNNNNGG 2 cut(s) 314, 412
BsnI GGCC 3 cut(s) 29, 101, 293
Bsp143I GATC 1 cut(s) 201
Bsp19I CCATGG 1 cut(s) 211
BspACI CCGC 2 cut(s) 67, 116
BspANI GGCC 3 cut(s) 29, 101, 293
BspFNI CGCG 1 cut(s) 116
BspLI GGNNCC 1 cut(s) 120
BspT107I GGYRCC 1 cut(s) 118
BsrFI RCCGGY 1 cut(s) 121
BssAI RCCGGY 1 cut(s) 121
BssECI CCNNGG 5 cut(s) 31, 96, 102, 171, 211
BssMI GATC 1 cut(s) 201
BssSI CACGAG 1 cut(s) 77
BssT1I CCWWGG 2 cut(s) 96, 211
Bst2BI CACGAG 1 cut(s) 77
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 1 cut(s) 411
BstDSI CCRYGG 2 cut(s) 102, 211
BstF5I GGATG 2 cut(s) 63, 348
BstFNI CGCG 1 cut(s) 116
BstKTI GATC 1 cut(s) 204
BstMBI GATC 1 cut(s) 201
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstNSI RCATGY 1 cut(s) 40
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 4 cut(s) 94, 166, 188, 258
BstXI CCANNNNNNTGG 1 cut(s) 103
BsuI GTATCC 1 cut(s) 210
BsuRI GGCC 3 cut(s) 29, 101, 293
BtgI CCRYGG 2 cut(s) 102, 211
BtsCI GGATG 2 cut(s) 63, 348
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 2 cut(s) 204, 396
Cac8I GCNNGC 1 cut(s) 38
Cfr10I RCCGGY 1 cut(s) 121
Cfr13I GGNCC 1 cut(s) 28
CviAII CATG 3 cut(s) 37, 212, 356
DdeI CTNAG 1 cut(s) 411
DpnI GATC 1 cut(s) 203
DpnII GATC 1 cut(s) 201
EciI GGCGGA 1 cut(s) 56
Eco130I CCWWGG 2 cut(s) 96, 211
Eco147I AGGCCT 1 cut(s) 293
EcoT14I CCWWGG 2 cut(s) 96, 211
ErhI CCWWGG 2 cut(s) 96, 211
FaeI CATG 3 cut(s) 40, 215, 359
FaiI YATR 7 cut(s) 38, 213, 267, 269, 297, 333, 357
FatI CATG 3 cut(s) 36, 211, 355
FbaI TGATCA 1 cut(s) 201
Fnu4HI GCNGC 4 cut(s) 83, 177, 180, 272
FokI GGATG 2 cut(s) 50, 355
Fsp4HI GCNGC 4 cut(s) 83, 177, 180, 272
FspBI CTAG 4 cut(s) 134, 312, 339, 421
GluI GCNGC 4 cut(s) 83, 177, 180, 272
GsaI CCCAGC 1 cut(s) 74
HaeIII GGCC 3 cut(s) 29, 101, 293
HapII CCGG 2 cut(s) 53, 122
Hin1II CATG 3 cut(s) 40, 215, 359
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HindIII AAGCTT 1 cut(s) 165
HpaI GTTAAC 1 cut(s) 418
HpaII CCGG 2 cut(s) 53, 122
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 2 cut(s) 172, 201
Hpy8I GTNNAC 1 cut(s) 418
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 1 cut(s) 241
HpyCH4V TGCA 2 cut(s) 4, 299
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
HpyF3I CTNAG 1 cut(s) 411
Hsp92II CATG 3 cut(s) 40, 215, 359
Ksp22I TGATCA 1 cut(s) 201
KspAI GTTAAC 1 cut(s) 418
Kzo9I GATC 1 cut(s) 201
LmnI GCTCC 1 cut(s) 406
LpnPI CCDG 7 cut(s) 5, 66, 84, 135, 234, 263, 321
Lsp1109I GCAGC 4 cut(s) 94, 166, 188, 258
MaeI CTAG 4 cut(s) 134, 312, 339, 421
MaeIII GTNAC 2 cut(s) 48, 240
MalI GATC 1 cut(s) 203
MboI GATC 1 cut(s) 201
MnlI CCTC 7 cut(s) 41, 100, 154, 166, 229, 274, 283
MseI TTAA 1 cut(s) 417
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 2 cut(s) 53, 122
MvnI CGCG 1 cut(s) 116
MwoI GCNNNNNNNGC 1 cut(s) 173
NcoI CCATGG 1 cut(s) 211
NdeII GATC 1 cut(s) 201
NlaIII CATG 3 cut(s) 40, 215, 359
NlaIV GGNNCC 1 cut(s) 120
NmeAIII GCCGAG 1 cut(s) 12
NspI RCATGY 1 cut(s) 40
PaeI GCATGC 1 cut(s) 40
PceI AGGCCT 1 cut(s) 293
PkrI GCNGC 4 cut(s) 84, 178, 181, 273
PspFI CCCAGC 1 cut(s) 70
PspN4I GGNNCC 1 cut(s) 120
PspPI GGNCC 1 cut(s) 28
PsrI GAACNNNNNNTAC 1 cut(s) 32
PvuII CAGCTG 1 cut(s) 179
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 4 cut(s) 83, 177, 180, 272
Sau3AI GATC 1 cut(s) 201
Sau96I GGNCC 1 cut(s) 28
SetI ASST 7 cut(s) 9, 169, 181, 226, 252, 369, 417
SphI GCATGC 1 cut(s) 40
SseBI AGGCCT 1 cut(s) 293
SsiI CCGC 2 cut(s) 67, 116
SspMI CTAG 4 cut(s) 134, 312, 339, 421
StuI AGGCCT 1 cut(s) 293
StyI CCWWGG 2 cut(s) 96, 211
TaqI TCGA 1 cut(s) 159
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TscAI CASTG 2 cut(s) 211, 403
TseI GCWGC 4 cut(s) 82, 176, 179, 271
TspDTI ATGAA 2 cut(s) 52, 216
TspRI CASTG 2 cut(s) 211, 403
XceI RCATGY 1 cut(s) 40
XspI CTAG 4 cut(s) 134, 312, 339, 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.