Rroxscaffold_1G00019230

mitogen-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015797
Physical Location & Seq
Forward (+)
23476647 .. 23479363
2717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00019230.1

Sequence Viewer

Length: 1098 bp
ATGGCTGACCTCCCTACCGGCAACGGCGACTTCCCGGCGGTTCCGTCGCACGGCGGCCAGTACATTCAGTACAACATTTTCGGCAACCTCTTCGAGATCACCAACAAGTACCGCCCTCCGATCATGCCGATCGGTCGCGGCGCCTACGGCATCGTCTGCTCCGTGTTGAATTCGGAGACGAACGAGATGGTGGCGATGAAGAAAATCGCCAACGCTTTTGATAATCACATGGACGCTAAGCGCACGCTCCGTGAGATTAAGCTGCTTCGTCATTTGGATCATGAAAATGTCATAGCTATTAGGGATGTGGTTCCTCCACCTCTAAGGAGAGAATTTTCAGATGTATACATTGCCACAGAGCTCATGGATACTGATCTGCATCAAATTATTCGCTCGAATCAGGTTTTATCAGAGGAGCATTGTCAGATTCTTCGAGGACTGAAATATATACATTCGGCAAATGTTATTCATAGGGACTTGAAGCCCAGCAACCTCTTGTTGAATGCTAATTGTGATCTGAAGATATGTGATTTTGGCCTTGCCCGTCCAACTGCTGAGAACGAGTTATTGACTGAGTATGTTGTCACCAGATGGTACAGGGCACCTGAGCTATTGTTGAACTCTTCAGATTATACTGCATCAATAGACGTTTGGTCTGTGGGCTGTATCTTTATGGAGCTTATGAATAGAAAGCCTCTATTTCCCGGCAAAGATCATGTGCATCAGATGCGCCTATTGACAGAGCTTCTTGGGACACCAACCGAGTCTGATCTTGGGTTTGTTCGGAATGAGGATGCCAGAAGATATATTCGGCAGCTTCCCCAGCATCCTCGTCAGCAAATGGTGAATCTTTTCCCACATGTGCATCCATTGGCCATTGATCTAGTGGATAGAATGTTGACCTTTGATCCCACTAAAAGGATAACTGTTGAAGAAGCATTAGCTCATCCTTACCTGGAAAGACTGCACGACGTAGCTGATGAACCAGCCTGTACTGTTCCATTCTCTTTTGATTTTGAGCAACAACCCTTGGGAGAAGAGCAAATGAAAGATATGATTTACAGAGAGGCTATAGGATTCAATCCTGAGTATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0006950 GO:0006970 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009314 GO:0009403 GO:0009404 GO:0009409 GO:0009411 GO:0009416 GO:0009555 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009791 GO:0009856 GO:0009908 GO:0009987 GO:0010033 GO:0010120 GO:0010183 GO:0010200 GO:0010224 GO:0010229 GO:0010243 GO:0010468 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0022414 GO:0023014 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033993 GO:0034641 GO:0035556 GO:0035670 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0042430 GO:0042435 GO:0042493 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044424 GO:0044464 GO:0044550 GO:0044706 GO:0046217 GO:0046483 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048583 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048868 GO:0050789 GO:0050794 GO:0050826 GO:0050896 GO:0050918 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0080134 GO:0080135 GO:0080136 GO:0090567 GO:0097305 GO:0097306 GO:0099402 GO:0140096 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

365

Amino Acids

41.95

Weight (kDa)

5.59

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 43 - 319 1.1e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 44 - 230 1.5e-28 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015797)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45640
fragaria_vesca FvH4_3g33900
malus_domestica MD03G1108500.v1.1 MD11G1121500.v1.1
prunus_persica Prupe.6G091700_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0061451
rosa_laevigata RLG00000035478
rosa_multiflora Rmu_sc0000671.1_g000003
rosa_roxburghii Rroxscaffold_1G00019230
rosa_rugosa Rorug05G0340400
rosa_samantha Rh5AG402700 Rh5BG415200 Rh5CG439900 Rh5DG429800
rosa_wichuraiana Rw5G037880

Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 140, 601
AccI GTMKAC 1 cut(s) 345
AccII CGCG 1 cut(s) 138
AciI CCGC 4 cut(s) 38, 54, 112, 138
AclWI GGATC 2 cut(s) 285, 902
AcoI YGGCCR 2 cut(s) 55, 873
AcsI RAATTY 2 cut(s) 169, 332
AcuI CTGAAG 2 cut(s) 539, 609
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 5 cut(s) 62, 71, 110, 596, 994
AfiI CCNNNNNNNGG 2 cut(s) 50, 918
AflIII ACRYGT 1 cut(s) 859
AgsI TTSAA 6 cut(s) 169, 481, 502, 619, 932, 1081
AhdI GACNNNNNGTC 1 cut(s) 652
AjnI CCWGG 1 cut(s) 954
AluBI AGCT 9 cut(s) 262, 296, 361, 610, 679, 745, 817, 944, 977
AluI AGCT 9 cut(s) 262, 296, 361, 610, 679, 745, 817, 944, 977
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 1 cut(s) 170
AlwI GGATC 2 cut(s) 285, 902
AoxI GGCC 3 cut(s) 55, 535, 873
ApeKI GCWGC 2 cut(s) 262, 814
ApoI RAATTY 2 cut(s) 169, 332
Asp700I GAANNNNTTC 1 cut(s) 851
AspLEI GCGC 3 cut(s) 143, 243, 732
AsuC2I CCSGG 2 cut(s) 35, 705
AsuHPI GGTGA 3 cut(s) 91, 577, 856
BaeGI GKGCMC 1 cut(s) 604
BalI TGGCCA 1 cut(s) 875
BanI GGYRCC 2 cut(s) 140, 601
BanII GRGCYC 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 2 cut(s) 249, 826
BccI CCATC 2 cut(s) 181, 585
BceAI ACGGC 3 cut(s) 40, 67, 163
BcgI CGANNNNNNTGC 2 cut(s) 73, 107
BciT130I CCWGG 1 cut(s) 956
BciVI GTATCC 1 cut(s) 361
BcnI CCSGG 2 cut(s) 35, 705
BcoDI GTCTC 1 cut(s) 170
BfaI CTAG 1 cut(s) 884
BfmI CTRYAG 1 cut(s) 1071
BfoI RGCGCY 1 cut(s) 144
BfuI GTATCC 1 cut(s) 361
BisI GCNGC 4 cut(s) 55, 139, 263, 815
BlpI GCTNAGC 1 cut(s) 237
BlsI GCNGC 4 cut(s) 56, 140, 264, 816
Bme1390I CCNGG 3 cut(s) 35, 705, 956
BmeRI GACNNNNNGTC 1 cut(s) 652
BmiI GGNNCC 4 cut(s) 42, 142, 312, 603
BmrFI CCNGG 3 cut(s) 35, 705, 956
BmsI GCATC 8 cut(s) 159, 388, 647, 717, 730, 784, 835, 874
Bpu10I CCTNAGC 1 cut(s) 606
Bpu1102I GCTNAGC 1 cut(s) 237
BpuMI CCSGG 2 cut(s) 35, 705
BsaBI GATNNNNATC 2 cut(s) 372, 378
BsaHI GRCGYC 1 cut(s) 141
BsaJI CCNNGG 1 cut(s) 1029
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 918
Bse118I RCCGGY 1 cut(s) 17
Bse1I ACTGG 1 cut(s) 58
Bse3DI GCAATG 1 cut(s) 348
Bse8I GATNNNNATC 2 cut(s) 372, 378
BseBI CCWGG 1 cut(s) 956
BseDI CCNNGG 1 cut(s) 1029
BseGI GGATG 5 cut(s) 310, 799, 826, 865, 946
BseJI GATNNNNATC 2 cut(s) 372, 378
BseLI CCNNNNNNNGG 2 cut(s) 50, 918
BseMI GCAATG 1 cut(s) 348
BseMII CTCAG 4 cut(s) 546, 564, 597, 1077
BseNI ACTGG 1 cut(s) 58
BseRI GAGGAG 1 cut(s) 428
BseSI GKGCMC 1 cut(s) 604
BseXI GCAGC 2 cut(s) 249, 826
BseYI CCCAGC 2 cut(s) 485, 822
BsgI GTGCAG 1 cut(s) 950
Bsh1236I CGCG 1 cut(s) 138
Bsh1285I CGRYCG 2 cut(s) 132, 136
BshFI GGCC 3 cut(s) 57, 537, 875
BshNI GGYRCC 2 cut(s) 140, 601
BsiEI CGRYCG 2 cut(s) 132, 136
BsiHKAI GWGCWC 1 cut(s) 363
BsiSI CCGG 3 cut(s) 18, 35, 705
BslFI GGGAC 2 cut(s) 488, 766
BslI CCNNNNNNNGG 2 cut(s) 50, 918
BsmAI GTCTC 1 cut(s) 170
BsmBI CGTCTC 1 cut(s) 170
BsmFI GGGAC 2 cut(s) 488, 766
BsmI GAATGC 1 cut(s) 508
BsnI GGCC 3 cut(s) 57, 537, 875
Bsp1286I GDGCHC 2 cut(s) 363, 604
Bsp1720I GCTNAGC 1 cut(s) 237
BspACI CCGC 4 cut(s) 38, 54, 112, 138
BspANI GGCC 3 cut(s) 57, 537, 875
BspCNI CTCAG 4 cut(s) 547, 565, 598, 1078
BspFNI CGCG 1 cut(s) 138
BspHI TCATGA 1 cut(s) 280
BspLI GGNNCC 4 cut(s) 42, 142, 312, 603
BspPI GGATC 2 cut(s) 285, 902
BspQI GCTCTTC 1 cut(s) 1032
BspT107I GGYRCC 2 cut(s) 140, 601
BsrDI GCAATG 1 cut(s) 348
BsrFI RCCGGY 1 cut(s) 17
BsrI ACTGG 1 cut(s) 58
BssAI RCCGGY 1 cut(s) 17
BssECI CCNNGG 1 cut(s) 1029
BssNAI GTATAC 1 cut(s) 346
BssNI GRCGYC 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 1029
Bst1107I GTATAC 1 cut(s) 346
Bst2UI CCWGG 1 cut(s) 956
Bst4CI ACNGT 2 cut(s) 928, 997
Bst6I CTCTTC 3 cut(s) 95, 628, 1032
BstACI GRCGYC 1 cut(s) 141
BstAPI GCANNNNNTGC 2 cut(s) 156, 727
BstC8I GCNNGC 1 cut(s) 245
BstDEI CTNAG 6 cut(s) 237, 323, 555, 573, 606, 1086
BstF5I GGATG 5 cut(s) 310, 799, 826, 865, 946
BstFNI CGCG 1 cut(s) 138
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 3 cut(s) 143, 243, 732
BstMAI GTCTC 1 cut(s) 170
BstMCI CGRYCG 2 cut(s) 132, 136
BstMWI GCNNNNNNNGC 4 cut(s) 147, 156, 727, 823
BstNI CCWGG 1 cut(s) 956
BstNSI RCATGY 1 cut(s) 863
BstSCI CCNGG 3 cut(s) 33, 703, 954
BstSFI CTRYAG 1 cut(s) 1071
BstSLI GKGCMC 1 cut(s) 604
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 2 cut(s) 249, 826
BstZ17I GTATAC 1 cut(s) 346
BsuI GTATCC 1 cut(s) 361
BsuRI GGCC 3 cut(s) 57, 537, 875
BtgZI GCGATG 1 cut(s) 209
BtsCI GGATG 5 cut(s) 310, 799, 826, 865, 946
Cac8I GCNNGC 1 cut(s) 245
CciI TCATGA 1 cut(s) 280
CfoI GCGC 3 cut(s) 143, 243, 732
Cfr10I RCCGGY 1 cut(s) 17
CseI GACGC 1 cut(s) 242
Csp6I GTAC 5 cut(s) 61, 70, 109, 595, 993
CviAII CATG 6 cut(s) 124, 229, 281, 364, 716, 860
CviQI GTAC 5 cut(s) 61, 70, 109, 595, 993
DdeI CTNAG 6 cut(s) 237, 323, 555, 573, 606, 1086
DinI GGCGCC 1 cut(s) 142
DriI GACNNNNNGTC 1 cut(s) 652
EaeI YGGCCR 2 cut(s) 55, 873
Eam1104I CTCTTC 3 cut(s) 95, 628, 1032
Eam1105I GACNNNNNGTC 1 cut(s) 652
EarI CTCTTC 3 cut(s) 95, 628, 1032
Ecl136II GAGCTC 1 cut(s) 361
Eco130I CCWWGG 1 cut(s) 1029
Eco24I GRGCYC 1 cut(s) 363
Eco53kI GAGCTC 1 cut(s) 361
Eco57I CTGAAG 2 cut(s) 539, 609
EcoICRI GAGCTC 1 cut(s) 361
EcoRI GAATTC 1 cut(s) 169
EcoRII CCWGG 1 cut(s) 954
EcoT14I CCWWGG 1 cut(s) 1029
EcoT38I GRGCYC 1 cut(s) 363
EgeI GGCGCC 1 cut(s) 142
EheI GGCGCC 1 cut(s) 142
ErhI CCWWGG 1 cut(s) 1029
Esp3I CGTCTC 1 cut(s) 170
FaeI CATG 6 cut(s) 127, 232, 284, 367, 719, 863
FaqI GGGAC 2 cut(s) 488, 766
FatI CATG 6 cut(s) 123, 228, 280, 363, 715, 859
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 4 cut(s) 55, 139, 263, 815
FokI GGATG 5 cut(s) 317, 806, 813, 852, 933
FriOI GRGCYC 1 cut(s) 363
Fsp4HI GCNGC 4 cut(s) 55, 139, 263, 815
FspBI CTAG 1 cut(s) 884
GlaI GCGC 3 cut(s) 142, 242, 731
GluI GCNGC 4 cut(s) 55, 139, 263, 815
GsaI CCCAGC 2 cut(s) 489, 826
HaeII RGCGCY 1 cut(s) 144
HaeIII GGCC 3 cut(s) 57, 537, 875
HapII CCGG 3 cut(s) 18, 35, 705
HgaI GACGC 1 cut(s) 242
HhaI GCGC 3 cut(s) 143, 243, 732
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 6 cut(s) 127, 232, 284, 367, 719, 863
Hin6I GCGC 3 cut(s) 141, 241, 730
HinP1I GCGC 3 cut(s) 141, 241, 730
HincII GTYRAC 1 cut(s) 900
HindII GTYRAC 1 cut(s) 900
HinfI GANTC 5 cut(s) 397, 427, 764, 847, 1077
HpaII CCGG 3 cut(s) 18, 35, 705
HphI GGTGA 3 cut(s) 91, 577, 856
Hpy166II GTNNAC 2 cut(s) 346, 900
Hpy188III TCNNGA 3 cut(s) 94, 281, 1085
Hpy8I GTNNAC 2 cut(s) 346, 900
Hpy99I CGWCG 2 cut(s) 49, 974
HpyCH4III ACNGT 2 cut(s) 928, 997
HpyCH4IV ACGT 2 cut(s) 648, 972
HpyCH4V TGCA 5 cut(s) 379, 638, 721, 865, 967
HpyF10VI GCNNNNNNNGC 4 cut(s) 147, 156, 727, 823
HpyF3I CTNAG 6 cut(s) 237, 323, 555, 573, 606, 1086
HpySE526I ACGT 2 cut(s) 648, 972
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 6 cut(s) 127, 232, 284, 367, 719, 863
HspAI GCGC 3 cut(s) 141, 241, 730
KasI GGCGCC 1 cut(s) 140
LguI GCTCTTC 1 cut(s) 1032
LmnI GCTCC 4 cut(s) 164, 252, 415, 676
Lsp1109I GCAGC 2 cut(s) 249, 826
LweI GCATC 8 cut(s) 159, 388, 647, 717, 730, 784, 835, 874
MaeI CTAG 1 cut(s) 884
MaeII ACGT 2 cut(s) 648, 972
MaeIII GTNAC 1 cut(s) 583
MboII GAAGA 8 cut(s) 82, 211, 422, 532, 615, 813, 944, 1049
MhlI GDGCHC 2 cut(s) 363, 604
MlsI TGGCCA 1 cut(s) 875
MluCI AATT 4 cut(s) 169, 332, 384, 508
MluNI TGGCCA 1 cut(s) 875
Mly113I GGCGCC 1 cut(s) 141
MlyI GAGTC 1 cut(s) 773
MmeI TCCRAC 1 cut(s) 572
Mox20I TGGCCA 1 cut(s) 875
MroXI GAANNNNTTC 1 cut(s) 851
MscI TGGCCA 1 cut(s) 875
MseI TTAA 1 cut(s) 258
MslI CAYNNNNRTG 1 cut(s) 285
Msp20I TGGCCA 1 cut(s) 875
MspI CCGG 3 cut(s) 18, 35, 705
MspR9I CCNGG 3 cut(s) 35, 705, 956
Mva1269I GAATGC 1 cut(s) 508
MvaI CCWGG 1 cut(s) 956
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 4 cut(s) 147, 156, 727, 823
NarI GGCGCC 1 cut(s) 141
NciI CCSGG 2 cut(s) 35, 705
NlaIII CATG 6 cut(s) 127, 232, 284, 367, 719, 863
NlaIV GGNNCC 4 cut(s) 42, 142, 312, 603
NmuCI GTSAC 1 cut(s) 583
NspI RCATGY 1 cut(s) 863
PagI TCATGA 1 cut(s) 280
PciI ACATGT 1 cut(s) 859
PciSI GCTCTTC 1 cut(s) 1032
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 1 cut(s) 508
PdmI GAANNNNTTC 1 cut(s) 851
PfeI GAWTC 4 cut(s) 397, 427, 847, 1077
PkrI GCNGC 4 cut(s) 56, 140, 264, 816
Ple19I CGATCG 1 cut(s) 132
PleI GAGTC 1 cut(s) 772
PluTI GGCGCC 1 cut(s) 144
PpsI GAGTC 1 cut(s) 772
PscI ACATGT 1 cut(s) 859
Psp124BI GAGCTC 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 954
PspFI CCCAGC 2 cut(s) 485, 822
PspGI CCWGG 1 cut(s) 954
PspN4I GGNNCC 4 cut(s) 42, 142, 312, 603
PvuI CGATCG 1 cut(s) 132
RsaI GTAC 5 cut(s) 62, 71, 110, 596, 994
RsaNI GTAC 5 cut(s) 61, 70, 109, 595, 993
RseI CAYNNNNRTG 1 cut(s) 285
SacI GAGCTC 1 cut(s) 363
SapI GCTCTTC 1 cut(s) 1032
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 4 cut(s) 55, 139, 263, 815
SchI GAGTC 1 cut(s) 773
ScrFI CCNGG 3 cut(s) 35, 705, 956
SduI GDGCHC 2 cut(s) 363, 604
SfaNI GCATC 8 cut(s) 159, 388, 647, 717, 730, 784, 835, 874
SfcI CTRYAG 1 cut(s) 1071
SfoI GGCGCC 1 cut(s) 142
SmiMI CAYNNNNRTG 1 cut(s) 285
Sse9I AATT 4 cut(s) 169, 332, 384, 508
SsiI CCGC 4 cut(s) 38, 54, 112, 138
SspDI GGCGCC 1 cut(s) 140
SspMI CTAG 1 cut(s) 884
SstI GAGCTC 1 cut(s) 363
StyD4I CCNGG 3 cut(s) 33, 703, 954
StyI CCWWGG 1 cut(s) 1029
TaaI ACNGT 2 cut(s) 928, 997
TaiI ACGT 2 cut(s) 651, 975
TaqI TCGA 3 cut(s) 93, 395, 433
TaqII GACCGA 1 cut(s) 122
TasI AATT 4 cut(s) 169, 332, 384, 508
TatI WGTACW 3 cut(s) 60, 69, 992
TauI GCSGC 2 cut(s) 57, 141
TfiI GAWTC 4 cut(s) 397, 427, 847, 1077
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TseFI GTSAC 1 cut(s) 583
TseI GCWGC 2 cut(s) 262, 814
Tsp45I GTSAC 1 cut(s) 583
TspDTI ATGAA 6 cut(s) 212, 297, 458, 698, 996, 1061
TspGWI ACGGA 3 cut(s) 33, 151, 239
XapI RAATTY 2 cut(s) 169, 332
XceI RCATGY 1 cut(s) 863
XcmI CCANNNNNNNNNTGG 2 cut(s) 361, 883
XmiI GTMKAC 1 cut(s) 345
XmnI GAANNNNTTC 1 cut(s) 851
XspI CTAG 1 cut(s) 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.