Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0007652

Physical Location & Seq

Genomic Coordinates Reverse (-)

26685863 .. 26693238

Gene Structure 7376 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00021700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2214 bp

ATGGGTACTTCCCAAAATGATCAAACAATGGAGGGTTGGCTTTATCTCATTCGTTCTAATCGGATTGGGTTACAGTACTCCCGCAAACGCTACTTTGTTCTTCAAGGCCATCTTCTTAAAAGCTTCAAATCACTCCCTGTTTCTGGCAATGAGGATCCCGTAAGAAGTGCTGTCATAGACGCATGCATCCGAGTGACTGATGATGGACGTGAGAGCATTAGTAGAAAAGTATTCTTCATATTCACACTGTATAATACGTCAAATCACAATGATCAACTTAAGTTGGGAGCAAGCAGTCCAGAAGAAGCCGCAAGATGGATTCATTCCTTTCAGGAAGTGGCTTTAAAGGCTGGTCCAGATCTTGGGAGTAATGTTTTGGAATGTTCAAGTAGCATATGGAAGTCCTTTAGATTAGGTGGATCTAATAGGGGAAGTCGTATTAGTTCTATAGACTGGACCCTTTGTTCTTCTACACAAACTGATGCTGTGACAGCCGATGTAATTGCTCCTTCACCTTGGACAATCTTTGGTTGTCAGAATGGTCTGCGACTATTTAAAGAAGCGAAAGATAGGGATTCTCATGGGAAGTGGGATGATCATCCTGCCATCATGGCAGTGGGTGTGGTTGGTGGAACTTCAGAAGCCATATTCCAGACACTTATGTCCCTTGGTCCCTCAAGATCTGAATGGGACTTCTGTTTCTACAAGGGCAGCGTGGTTGAACACCTTGACGGTCACACTGATATCATTCACAAGCAGTTATATAGCGATTGGATGCCATGGGGAATGAAGCCAAGAGATCTATTGTTGCGGCGGTATTGGAGAAGGGAAGATGATGGGACATATGTTATTCTCTACCATTCTGTGTTCCATAAGAAGTGTCCATCTCAGAAAGGCTACGTCCGCGCCTGCCTTAAAAGTGGAGGATATGTTATATCACCTGTGAACCAAGGGAAACATTCAGTTGTGAAGCACATGCTTGCAATTGATTGGAAGTACTGGAAATCTTACCTGCAGACTTCTTCATCCCGATCTATGACCATCAAAGTGCTAGGAAGAGTTGCTGCACTACGAGAGTTATTCAAAGCAAAAGTAGGAAACTATTCTTCCTCAGAGTTTTCATCTGGAGAGCTAAGGAGAAATTTTGGTGTGCATCAAAATGAAGGGTATGGAGAGGCTGATGCTCTGGCACTGACTGGGGATGGGAGCACTAATGAAAATATATTGGAAGAAGTGGATAAATCACCTTCAGAACATGCTAGTCTTGTTAGCTTGAATGATGCTTCTGATGAATTCTTTGATGTGCCTGAGCCTCCATACCATGATCAATCAGAAACTGACTGGACTCCTGATTTTGGCTCTGATATATATTCACAGGAATTGCAGGATACACGTCATCCTAAGTTATCAACTGCTGCTGGTTTTGTGAAGAGATTGCATGATCTTACAGTTCAGAAGAGGGGCTATGTGGACCTACAAGAGATGACCAGGGAAGACAGTATGTCATGCAACTATGGGTTCACACTTCCAAAAGATTCAACTTGTTCTTTGGCTTGCAGTTGGACTGAGACAGACCCTTCCACTTTTCTAATTCGTGGCAAAAGTTATCTGGAAGACCGTAAGAAGGTTAAAGCAAAGGGCACGTTGATGCAAATGGTTGCTGCAGATTGGCTAAGATCTGACAAGCGTGAAGATGATCTTGGAGGTCGTCCTGGGAGCATTGTTCAGAAATATGCAGCAAAGGGTGGGAAAGAATTCTTCTTCATTGTGAACATTCAGGTTCCAGGTTCAACCACATATAGCCTAGTCCTATACTATATGATGAGTTCTCCAGTTGAAGATGCACCCTTGCTAGAGAATTTTGTTAAGGGGGATGATGCATATAGAAATTCAAGGTTTAAGCTCATACCATACATATCTAAGGGTTCATGGATTGTTAAGCAGAGTGTGGGAAAGAAGGCATGCATCATTGGTCAAGCACTTCAAATAAATTACTTCCATGGGAAGAACTACGTGGAGCTTGGTATTGACATTGGATCATCGACAGTCGCAAGGGGTGTGGTTAGTCTTGTCCTCGGGTACCTAAACAATCTTGTGATTGAGATGGCATTTTTGATACAGGCAAATACGCCAGAGGAGCTTCCAGAGTATCTTCTTGGGACCTGCCGCCTTAATCATCTTGATGCCTCTAAGGCCATTTTAGTGAAACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1336 PH START

Protein Analysis

737

Amino Acids

82.66

Weight (kDa)

7.27

Isoelectric Point (pI)

45.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH	PF00169	9 - 113	3.8e-08	PH domain
START	PF01852	200 - 340	3.7e-20	START domain
EDR2_C	PF07059	521 - 728	1.3e-76	Protein ENHANCED DISEASE RESISTANCE 2, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007652)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G28320
fragaria_vesca	FvH4_3g31950 FvH4_3g31950 FvH4_3g31950 FvH4_3g31950 FvH4_3g31950 FvH4_3g31950
malus_domestica	MD11G1137600.v1.1
prunus_persica	Prupe.6G103400_v2.0.a1 Prupe.6G103400_v2.0.a1 Prupe.6G103400_v2.0.a1 Prupe.6G103400_v2.0.a1
pyrus_communis	pycom11g11400
rosa_chinensis	RchiOBHm_Chr5g0058701
rosa_laevigata	RLG00000008103 RLG00000035242
rosa_multiflora	Rmu_sc0000367.1_g000218
rosa_roxburghii	Rroxscaffold_1G00021700
rosa_rugosa	Rorug05G0317500
rosa_samantha	Rh5AG383200 Rh5BG395100 Rh5CG418900 Rh5DG410600
rosa_wichuraiana	Rw5G036110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	661
Acc36I	ACCTGC	2 cut(s)	1020, 2171
Acc65I	GGTACC	1 cut(s)	2079
AccB1I	GGYRCC	1 cut(s)	2079
AccB7I	CCANNNNNTGG	1 cut(s)	362
AccII	CGCG	1 cut(s)	906
AciI	CCGC	6 cut(s)	82, 309, 811, 814, 904, 2167
AclWI	GGATC	4 cut(s)	149, 162, 427, 2044
AcsI	RAATTY	5 cut(s)	1141, 1292, 1754, 1858, 1888
AcuI	CTGAAG	2 cut(s)	621, 1233
AfaI	GTAC	4 cut(s)	7, 77, 998, 2081
AfiI	CCNNNNNNNGG	5 cut(s)	143, 315, 362, 1355, 1624
AflII	CTTAAG	1 cut(s)	278
AflIII	ACRYGT	1 cut(s)	1391
AhdI	GACNNNNNGTC	1 cut(s)	1501
AjiI	CACGTC	2 cut(s)	209, 1394
AjnI	CCWGG	3 cut(s)	1487, 1711, 1783
AjuI	GAANNNNNNNTTGG	2 cut(s)	1683, 1715
AloI	GAACNNNNNNTCC	2 cut(s)	448, 480
AluBI	AGCT	6 cut(s)	123, 1132, 1272, 1903, 2020, 2140
AluI	AGCT	6 cut(s)	123, 1132, 1272, 1903, 2020, 2140
Alw21I	GWGCWC	1 cut(s)	1211
Alw26I	GTCTC	1 cut(s)	1562
AlwI	GGATC	4 cut(s)	149, 162, 427, 2044
AlwNI	CAGNNNCTG	1 cut(s)	1337
Ama87I	CYCGRG	1 cut(s)	2075
AoxI	GGCC	2 cut(s)	106, 2193
ApeKI	GCWGC	5 cut(s)	711, 1064, 1415, 1661, 1736
ApoI	RAATTY	5 cut(s)	1141, 1292, 1754, 1858, 1888
Asp700I	GAANNNNTTC	2 cut(s)	1102, 1754
Asp718I	GGTACC	1 cut(s)	2079
AspLEI	GCGC	1 cut(s)	908
AspS9I	GGNCC	5 cut(s)	353, 456, 671, 1471, 2160
AsuHPI	GGTGA	3 cut(s)	504, 930, 1236
AvaI	CYCGRG	1 cut(s)	2075
AvaII	GGWCC	5 cut(s)	353, 456, 671, 1471, 2160
BaeGI	GKGCMC	1 cut(s)	1643
BamHI	GGATCC	1 cut(s)	154
BanI	GGYRCC	1 cut(s)	2079
BbsI	GAAGAC	2 cut(s)	1500, 1620
Bbv12I	GWGCWC	1 cut(s)	1211
BbvI	GCAGC	5 cut(s)	723, 1051, 1402, 1648, 1748
BccI	CCATC	9 cut(s)	117, 197, 309, 614, 830, 892, 1049, 1196, 2098
BcgI	CGANNNNNNTGC	2 cut(s)	485, 519
BciT130I	CCWGG	3 cut(s)	1489, 1713, 1785
BciVI	GTATCC	1 cut(s)	1381
BclI	TGATCA	4 cut(s)	19, 271, 595, 1324
BcoDI	GTCTC	1 cut(s)	1562
BfaI	CTAG	4 cut(s)	1052, 1260, 1805, 1853
BfmI	CTRYAG	3 cut(s)	447, 1013, 1662
BfrI	CTTAAG	1 cut(s)	278
BfuAI	ACCTGC	2 cut(s)	1020, 2171
BfuI	GTATCC	1 cut(s)	1381
BglI	GCCNNNNNGGC	2 cut(s)	611, 2192
BglII	AGATCT	4 cut(s)	358, 680, 799, 1676
BisI	GCNGC	8 cut(s)	309, 712, 812, 1065, 1416, 1662, 1737, 2167
BlsI	GCNGC	8 cut(s)	310, 713, 813, 1066, 1417, 1663, 1738, 2168
BmcAI	AGTACT	2 cut(s)	77, 998
Bme1390I	CCNGG	3 cut(s)	1489, 1713, 1785
Bme18I	GGWCC	5 cut(s)	353, 456, 671, 1471, 2160
BmeRI	GACNNNNNGTC	1 cut(s)	1501
BmeT110I	CYCGRG	1 cut(s)	2075
BmgBI	CACGTC	2 cut(s)	209, 1394
BmgT120I	GGNCC	5 cut(s)	353, 456, 671, 1471, 2160
BmiI	GGNNCC	6 cut(s)	156, 458, 673, 1782, 2081, 2161
BmrFI	CCNGG	3 cut(s)	1489, 1713, 1785
BmrI	ACTGGG	1 cut(s)	1206
BmuI	ACTGGG	1 cut(s)	1206
BpiI	GAAGAC	2 cut(s)	1500, 1620
BpmI	CTGGAG	2 cut(s)	1146, 1815
Bpu10I	CCTNAGC	2 cut(s)	1133, 1308
BpuEI	CTTGAG	1 cut(s)	661
BsaAI	YACGTR	1 cut(s)	2014
BsaBI	GATNNNNATC	1 cut(s)	597
BsaJI	CCNNGG	8 cut(s)	515, 667, 779, 949, 1488, 1712, 1999, 2074
BsaXI	ACNNNNNCTCC	2 cut(s)	915, 945
Bsc4I	CCNNNNNNNGG	5 cut(s)	143, 315, 362, 1355, 1624
Bse1I	ACTGG	5 cut(s)	458, 1004, 1201, 1346, 1832
Bse3DI	GCAATG	1 cut(s)	154
Bse8I	GATNNNNATC	1 cut(s)	597
BseBI	CCWGG	3 cut(s)	1489, 1713, 1785
BseDI	CCNNGG	8 cut(s)	515, 667, 779, 949, 1488, 1712, 1999, 2074
BseGI	GGATG	8 cut(s)	186, 598, 598, 780, 1025, 1207, 1396, 1879
BseJI	GATNNNNATC	1 cut(s)	597
BseLI	CCNNNNNNNGG	5 cut(s)	143, 315, 362, 1355, 1624
BseMI	GCAATG	1 cut(s)	154
BseMII	CTCAG	4 cut(s)	902, 1125, 1299, 1557
BseNI	ACTGG	5 cut(s)	458, 1004, 1201, 1346, 1832
BseRI	GAGGAG	1 cut(s)	2150
BseSI	GKGCMC	1 cut(s)	1643
BseXI	GCAGC	5 cut(s)	723, 1051, 1402, 1648, 1748
BsgI	GTGCAG	1 cut(s)	1050
Bsh1236I	CGCG	1 cut(s)	906
BshFI	GGCC	2 cut(s)	108, 2195
BshNI	GGYRCC	1 cut(s)	2079
BsiHKAI	GWGCWC	1 cut(s)	1211
BsiHKCI	CYCGRG	1 cut(s)	2075
BslFI	GGGAC	5 cut(s)	649, 657, 704, 853, 2173
BslI	CCNNNNNNNGG	5 cut(s)	143, 315, 362, 1355, 1624
BsmAI	GTCTC	1 cut(s)	1562
BsmFI	GGGAC	5 cut(s)	649, 657, 704, 853, 2173
BsnI	GGCC	2 cut(s)	108, 2195
BsoBI	CYCGRG	1 cut(s)	2075
Bsp1286I	GDGCHC	2 cut(s)	1211, 1643
Bsp19I	CCATGG	2 cut(s)	779, 1999
BspACI	CCGC	6 cut(s)	82, 309, 811, 814, 904, 2167
BspANI	GGCC	2 cut(s)	108, 2195
BspCNI	CTCAG	4 cut(s)	901, 1124, 1300, 1558
BspFNI	CGCG	1 cut(s)	906
BspLI	GGNNCC	6 cut(s)	156, 458, 673, 1782, 2081, 2161
BspMAI	CTGCAG	2 cut(s)	1017, 1666
BspMI	ACCTGC	2 cut(s)	1020, 2171
BspPI	GGATC	4 cut(s)	149, 162, 427, 2044
BspT107I	GGYRCC	1 cut(s)	2079
BspTI	CTTAAG	1 cut(s)	278
BsrDI	GCAATG	1 cut(s)	154
BsrI	ACTGG	5 cut(s)	458, 1004, 1201, 1346, 1832
BssECI	CCNNGG	8 cut(s)	515, 667, 779, 949, 1488, 1712, 1999, 2074
BssT1I	CCWWGG	5 cut(s)	515, 667, 779, 949, 1999
Bst2UI	CCWGG	3 cut(s)	1489, 1713, 1785
Bst4CI	ACNGT	7 cut(s)	75, 249, 734, 1450, 1499, 1619, 2047
Bst6I	CTCTTC	3 cut(s)	1051, 1424, 1451
BstAFI	CTTAAG	1 cut(s)	278
BstBAI	YACGTR	1 cut(s)	2014
BstC8I	GCNNGC	6 cut(s)	184, 292, 910, 981, 1555, 1963
BstDEI	CTNAG	9 cut(s)	888, 1111, 1133, 1308, 1401, 1566, 1673, 1920, 2190
BstDSI	CCRYGG	2 cut(s)	779, 1999
BstF5I	GGATG	8 cut(s)	186, 598, 598, 780, 1025, 1207, 1396, 1879
BstFNI	CGCG	1 cut(s)	906
BstHHI	GCGC	1 cut(s)	908
BstMAI	GTCTC	1 cut(s)	1562
BstMWI	GCNNNNNNNGC	6 cut(s)	347, 491, 611, 903, 2137, 2192
BstNI	CCWGG	3 cut(s)	1489, 1713, 1785
BstNSI	RCATGY	4 cut(s)	186, 979, 1259, 1965
BstSCI	CCNGG	3 cut(s)	1487, 1711, 1783
BstSFI	CTRYAG	3 cut(s)	447, 1013, 1662
BstSLI	GKGCMC	1 cut(s)	1643
BstUI	CGCG	1 cut(s)	906
BstV1I	GCAGC	5 cut(s)	723, 1051, 1402, 1648, 1748
BstV2I	GAAGAC	2 cut(s)	1500, 1620
BstX2I	RGATCY	6 cut(s)	154, 358, 419, 680, 799, 1676
BstYI	RGATCY	6 cut(s)	154, 358, 419, 680, 799, 1676
BsuI	GTATCC	1 cut(s)	1381
BsuRI	GGCC	2 cut(s)	108, 2195
BtgI	CCRYGG	2 cut(s)	779, 1999
BtrI	CACGTC	2 cut(s)	209, 1394
BtsCI	GGATG	8 cut(s)	186, 598, 598, 780, 1025, 1207, 1396, 1879
BtsI	GCAGTG	1 cut(s)	621
BtsIMutI	CAGTG	4 cut(s)	245, 621, 738, 1190
BveI	ACCTGC	2 cut(s)	1020, 2171
Cac8I	GCNNGC	6 cut(s)	184, 292, 910, 981, 1555, 1963
CaiI	CAGNNNCTG	1 cut(s)	1337
CfoI	GCGC	1 cut(s)	908
Cfr13I	GGNCC	5 cut(s)	353, 456, 671, 1471, 2160
CseI	GACGC	1 cut(s)	188
Csp6I	GTAC	4 cut(s)	6, 76, 997, 2080
CspCI	CAANNNNNGTGG	2 cut(s)	2040, 2075
CviQI	GTAC	4 cut(s)	6, 76, 997, 2080
DdeI	CTNAG	9 cut(s)	888, 1111, 1133, 1308, 1401, 1566, 1673, 1920, 2190
DraI	TTTAAA	2 cut(s)	345, 556
DrdI	GACNNNNNNGTC	1 cut(s)	661
DriI	GACNNNNNGTC	1 cut(s)	1501
DseDI	GACNNNNNNGTC	1 cut(s)	661
Eam1104I	CTCTTC	3 cut(s)	1051, 1424, 1451
Eam1105I	GACNNNNNGTC	1 cut(s)	1501
EarI	CTCTTC	3 cut(s)	1051, 1424, 1451
Eco130I	CCWWGG	5 cut(s)	515, 667, 779, 949, 1999
Eco32I	GATATC	1 cut(s)	745
Eco47I	GGWCC	5 cut(s)	353, 456, 671, 1471, 2160
Eco57I	CTGAAG	2 cut(s)	621, 1233
Eco88I	CYCGRG	1 cut(s)	2075
EcoO109I	RGGNCCY	1 cut(s)	2160
EcoRI	GAATTC	2 cut(s)	1292, 1754
EcoRII	CCWGG	3 cut(s)	1487, 1711, 1783
EcoRV	GATATC	1 cut(s)	745
EcoT14I	CCWWGG	5 cut(s)	515, 667, 779, 949, 1999
EcoT22I	ATGCAT	3 cut(s)	188, 1882, 1967
ErhI	CCWWGG	5 cut(s)	515, 667, 779, 949, 1999
FalI	AAGNNNNNCTT	4 cut(s)	96, 128, 1683, 1715
FaqI	GGGAC	5 cut(s)	649, 657, 704, 853, 2173
FauI	CCCGC	1 cut(s)	89
FauNDI	CATATG	2 cut(s)	395, 844
FbaI	TGATCA	4 cut(s)	19, 271, 595, 1324
Fnu4HI	GCNGC	8 cut(s)	309, 712, 812, 1065, 1416, 1662, 1737, 2167
FokI	GGATG	8 cut(s)	173, 585, 605, 787, 1012, 1214, 1383, 1886
Fsp4HI	GCNGC	8 cut(s)	309, 712, 812, 1065, 1416, 1662, 1737, 2167
FspBI	CTAG	4 cut(s)	1052, 1260, 1805, 1853
GlaI	GCGC	1 cut(s)	907
GluI	GCNGC	8 cut(s)	309, 712, 812, 1065, 1416, 1662, 1737, 2167
GsuI	CTGGAG	2 cut(s)	1146, 1815
HaeIII	GGCC	2 cut(s)	108, 2195
HgaI	GACGC	1 cut(s)	188
HhaI	GCGC	1 cut(s)	908
Hin6I	GCGC	1 cut(s)	906
HinP1I	GCGC	1 cut(s)	906
HindIII	AAGCTT	1 cut(s)	121
HinfI	GANTC	4 cut(s)	319, 575, 1345, 1535
HphI	GGTGA	3 cut(s)	504, 930, 1236
Hpy166II	GTNNAC	4 cut(s)	946, 1471, 1521, 1771
Hpy8I	GTNNAC	4 cut(s)	946, 1471, 1521, 1771
HpyAV	CCTTC	7 cut(s)	519, 819, 1157, 1257, 1587, 1618, 1951
HpyCH4III	ACNGT	7 cut(s)	75, 249, 734, 1450, 1499, 1619, 2047
HpyCH4IV	ACGT	6 cut(s)	208, 257, 900, 1393, 1643, 2013
HpyF10VI	GCNNNNNNNGC	6 cut(s)	347, 491, 611, 903, 2137, 2192
HpyF3I	CTNAG	9 cut(s)	888, 1111, 1133, 1308, 1401, 1566, 1673, 1920, 2190
HpySE526I	ACGT	6 cut(s)	208, 257, 900, 1393, 1643, 2013
HspAI	GCGC	1 cut(s)	906
KpnI	GGTACC	1 cut(s)	2083
Ksp22I	TGATCA	4 cut(s)	19, 271, 595, 1324
LmnI	GCTCC	6 cut(s)	287, 511, 1206, 1716, 2017, 2137
Lsp1109I	GCAGC	5 cut(s)	723, 1051, 1402, 1648, 1748
MaeI	CTAG	4 cut(s)	1052, 1260, 1805, 1853
MaeII	ACGT	6 cut(s)	208, 257, 900, 1393, 1643, 2013
MaeIII	GTNAC	4 cut(s)	69, 193, 487, 734
MfeI	CAATTG	1 cut(s)	984
MflI	RGATCY	6 cut(s)	154, 358, 419, 680, 799, 1676
MhlI	GDGCHC	2 cut(s)	1211, 1643
MlyI	GAGTC	1 cut(s)	1339
MmeI	TCCRAC	1 cut(s)	1541
Mph1103I	ATGCAT	3 cut(s)	188, 1882, 1967
MroXI	GAANNNNTTC	2 cut(s)	1102, 1754
MslI	CAYNNNNRTG	7 cut(s)	191, 614, 1046, 1158, 1646, 2181, 2201
MspCI	CTTAAG	1 cut(s)	278
MspR9I	CCNGG	3 cut(s)	1489, 1713, 1785
MunI	CAATTG	1 cut(s)	984
MvaI	CCWGG	3 cut(s)	1489, 1713, 1785
MvnI	CGCG	1 cut(s)	906
MwoI	GCNNNNNNNGC	6 cut(s)	347, 491, 611, 903, 2137, 2192
NcoI	CCATGG	2 cut(s)	779, 1999
NdeI	CATATG	2 cut(s)	395, 844
NlaIV	GGNNCC	6 cut(s)	156, 458, 673, 1782, 2081, 2161
NmuCI	GTSAC	3 cut(s)	193, 487, 734
NsiI	ATGCAT	3 cut(s)	188, 1882, 1967
NspI	RCATGY	4 cut(s)	186, 979, 1259, 1965
PaeI	GCATGC	2 cut(s)	186, 1965
PdmI	GAANNNNTTC	2 cut(s)	1102, 1754
PfeI	GAWTC	3 cut(s)	319, 575, 1535
PflMI	CCANNNNNTGG	1 cut(s)	362
PkrI	GCNGC	8 cut(s)	310, 713, 813, 1066, 1417, 1663, 1738, 2168
PleI	GAGTC	1 cut(s)	1339
PpsI	GAGTC	1 cut(s)	1339
Ppu21I	YACGTR	1 cut(s)	2014
PpuMI	RGGWCCY	1 cut(s)	2160
Psp5II	RGGWCCY	1 cut(s)	2160
Psp6I	CCWGG	3 cut(s)	1487, 1711, 1783
PspGI	CCWGG	3 cut(s)	1487, 1711, 1783
PspN4I	GGNNCC	6 cut(s)	156, 458, 673, 1782, 2081, 2161
PspPI	GGNCC	5 cut(s)	353, 456, 671, 1471, 2160
PspPPI	RGGWCCY	1 cut(s)	2160
PstI	CTGCAG	2 cut(s)	1017, 1666
PstNI	CAGNNNCTG	1 cut(s)	1337
PsuI	RGATCY	6 cut(s)	154, 358, 419, 680, 799, 1676
RsaI	GTAC	4 cut(s)	7, 77, 998, 2081
RsaNI	GTAC	4 cut(s)	6, 76, 997, 2080
RseI	CAYNNNNRTG	7 cut(s)	191, 614, 1046, 1158, 1646, 2181, 2201
SatI	GCNGC	8 cut(s)	309, 712, 812, 1065, 1416, 1662, 1737, 2167
Sau96I	GGNCC	5 cut(s)	353, 456, 671, 1471, 2160
ScaI	AGTACT	2 cut(s)	77, 998
SchI	GAGTC	1 cut(s)	1339
ScrFI	CCNGG	3 cut(s)	1489, 1713, 1785
SduI	GDGCHC	2 cut(s)	1211, 1643
SfcI	CTRYAG	3 cut(s)	447, 1013, 1662
SinI	GGWCC	5 cut(s)	353, 456, 671, 1471, 2160
SmiMI	CAYNNNNRTG	7 cut(s)	191, 614, 1046, 1158, 1646, 2181, 2201
SmlI	CTYRAG	2 cut(s)	278, 676
SmoI	CTYRAG	2 cut(s)	278, 676
SphI	GCATGC	2 cut(s)	186, 1965
SsiI	CCGC	6 cut(s)	82, 309, 811, 814, 904, 2167
SspMI	CTAG	4 cut(s)	1052, 1260, 1805, 1853
StyD4I	CCNGG	3 cut(s)	1487, 1711, 1783
StyI	CCWWGG	5 cut(s)	515, 667, 779, 949, 1999
TaaI	ACNGT	7 cut(s)	75, 249, 734, 1450, 1499, 1619, 2047
TaiI	ACGT	6 cut(s)	211, 260, 903, 1396, 1646, 2016
TaqI	TCGA	1 cut(s)	2042
TatI	WGTACW	2 cut(s)	75, 996
TauI	GCSGC	3 cut(s)	311, 814, 2169
TfiI	GAWTC	3 cut(s)	319, 575, 1535
TscAI	CASTG	4 cut(s)	252, 621, 745, 1197
TseFI	GTSAC	3 cut(s)	193, 487, 734
TseI	GCWGC	5 cut(s)	711, 1064, 1415, 1661, 1736
Tsp45I	GTSAC	3 cut(s)	193, 487, 734
TspRI	CASTG	4 cut(s)	252, 621, 745, 1197
Van91I	CCANNNNNTGG	1 cut(s)	362
Vha464I	CTTAAG	1 cut(s)	278
VpaK11BI	GGWCC	5 cut(s)	353, 456, 671, 1471, 2160
XapI	RAATTY	5 cut(s)	1141, 1292, 1754, 1858, 1888
XceI	RCATGY	4 cut(s)	186, 979, 1259, 1965
XcmI	CCANNNNNNNNNTGG	1 cut(s)	613
XmnI	GAANNNNTTC	2 cut(s)	1102, 1754
XspI	CTAG	4 cut(s)	1052, 1260, 1805, 1853
ZrmI	AGTACT	2 cut(s)	77, 998
Zsp2I	ATGCAT	3 cut(s)	188, 1882, 1967

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.