Rroxscaffold_1G00037170

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0025630
Physical Location & Seq
Forward (+)
54254539 .. 54259804
5266 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00037170.1

Sequence Viewer

Length: 1866 bp
ATGCAAGAAGCCAGAGTCGCAACACATTTAGTGGTCAAGGCCCTCAAAGATTCTAGCTTGCTTTTGGATGCTGATGATGACAGATGTGTAAGGATGCATGATGTCATTCGGGATGTGGCAATACTAATTTCTTTATCTAAAGACGGCCAACGGTTTTTGGTAAAATCAGGATGTGAATTAAAGAGTTGGCCAATGATTGCTGCACACAATGACTATTCTGCAATCTCCCTAATGAAGAACGAAATTCGCAAGCTACCCGAAGAGTTGGTATGTCCAAACCTTCAAATTTTATTACTACAACAGAATTCTGATTTAAATGAGATTCCAGAAACATTTTTCCAAAGTCCAAATGAATTAAAGGTCTTAGATCTTAGCCACACTAGAATATCGTTCCTACCCCAGTCATTCAGTCTCCTAACTAACCTCCAGACTTTGTATTTAGATTGTTGCTGGAAAGTAATGGACGTTTCTATTTTCAAAAACCTTAAAAAGCTAGAGATTCTTAGTATGAGATGTTATCCCCTTGAGGAATTGTCGAGAGATATAGGACAATTGACCAATCTAAGGATGCTAGATGTCAGTGCTAGTAGATTTTCGGTTGGACATATTGTTAGAATTCCATCAAAAGTGATATCAAAGTTGCATAGATTAGAAGAACTGTACATGCAATGTGGATTTTTGTACTGGGGGAGTCAAATTGAGGGAGAAGGAGAAGAAACCAATATTGGCTTTGATGAGGTGACTGGGTTATCGTATTTAAACATTTTGAAGGTTTGCATATCTGATGCAAAATGCATCCCTAAAAGTGTTGAGTTCGAACCGAGTTGGGCTCACTTTGATATATGTATTGGCAGACGAAAAGAGCCGGAAGTTTCTGGATATGGTCCTAATTATTCAAGATCCTTGATTCTTGACACAACCATCAGTACCTTACCAGATTGGTTTATCAATGTAGTGACAAAGCAAACAGAAAAGCTACAATATGAACTTCGCAAAGGGTTTACTGGGTTTAATAACATTCTCGTGGAATACGACCATGGGAGATTACATGGACTGAAGGTTCTCTCTGTGATTGGTCCCAATGAGAACTTGAAAGAGTTGATGAACTCAATAACATGGGTTCCAAAAGAACCTGTGTTCAAGAACTTGGAAGAGTTGCATCTGGAATACCTGTATGGCTTGAAGGAGCTATCTGTTGATGAATTACCACCCGGATCTCTATTCAATCTGAAGAAACTGAGGATCCGTAATTGTCATAACTTGGAGGTACTGTTGCCTTCAAAACTGTTACAAAGAGTACCGAATCTGGAAGTTCTAGTTTGTGATGGGATGCGTAAAATGGAGCATGTGTTTGGATGTGAAGGGATTGAGATAAAACAATTAAAATTGAGAGTGATGAAATTGTTTAGTTTACTTTCAGTAAGAAGCATATGTAATGGTCCTGCTCCACCTGCAATGTTCCAGACTCTTAAAAGTTTGTACATTTTCGAATGCGAGATTCCAGGAAGTCTCTTCACATCTAATGCAGCTCAGTGTCTTCTTCAATTGGAAGACCTTTGGATAGAAAAATGCCATTTGTTGGAAAGAGTAATCGAAGTGGTGAACAACAACAAGAAAACTGTTGTTCCGAGATTGAAGAACCTGGTTTTGAGAGATCTACCAAGGTTGTACTGTGGAAATGCTACTGCTGATATTGAGTGTCCTTCATTGGAACACTTGTACGTGAATGACTGCCCCCATTTGTTATTTTCAACCTCCACTTCCGACTATTTCCAGAGCAGGAACCAGGTCCAACTGACTTCATATTCCGACATGTTTCTTATTACTAGATCTATGGAAGACATGTTCCCAATAGAAGATTGGTGA

Protein Analysis

621

Amino Acids

71.24

Weight (kDa)

5.84

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 92 - 148 6.9e-11 Leucine rich repeat
LRR_14 PF23598 104 - 226 2.5e-12 Leucine-rich repeat region
LRR_RPS2 PF23247 383 - 524 3.4e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 472 - 587 4.7e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0025630)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0009227.1_g000013 Rmu_sc0010851.1_g000006
rosa_roxburghii Rroxscaffold_1G00037170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1459
Acc36I ACCTGC 1 cut(s) 1459
AccB7I CCANNNNNTGG 1 cut(s) 1579
AclWI GGATC 4 cut(s) 894, 1222, 1237, 1250
AcoI YGGCCR 2 cut(s) 145, 188
AcsI RAATTY 4 cut(s) 243, 285, 304, 615
AcuI CTGAAG 2 cut(s) 1076, 1250
AfaI GTAC 8 cut(s) 662, 683, 928, 1269, 1299, 1481, 1670, 1721
AfiI CCNNNNNNNGG 2 cut(s) 564, 1579
AflIII ACRYGT 2 cut(s) 1812, 1842
AjnI CCWGG 3 cut(s) 1501, 1641, 1785
AjuI GAANNNNNNNTTGG 2 cut(s) 708, 740
AloI GAACNNNNNNTCC 4 cut(s) 1044, 1076, 1829, 1861
AluBI AGCT 6 cut(s) 57, 253, 493, 976, 1189, 1529
AluI AGCT 6 cut(s) 57, 253, 493, 976, 1189, 1529
Alw26I GTCTC 2 cut(s) 416, 1514
AlwI GGATC 4 cut(s) 894, 1222, 1237, 1250
AoxI GGCC 3 cut(s) 39, 145, 188
ApeKI GCWGC 2 cut(s) 200, 1526
ApoI RAATTY 4 cut(s) 243, 285, 304, 615
AspS9I GGNCC 5 cut(s) 40, 884, 1076, 1439, 1789
AsuC2I CCSGG 1 cut(s) 1212
AsuHPI GGTGA 2 cut(s) 751, 1612
AsuII TTCGAA 2 cut(s) 816, 1488
AvaII GGWCC 4 cut(s) 884, 1076, 1439, 1789
BalI TGGCCA 1 cut(s) 190
BamHI GGATCC 1 cut(s) 1242
BanII GRGCYC 1 cut(s) 832
BauI CACGAG 1 cut(s) 1022
BbsI GAAGAC 3 cut(s) 1529, 1557, 1845
BbvI GCAGC 2 cut(s) 187, 1538
BccI CCATC 3 cut(s) 628, 929, 1319
BceAI ACGGC 1 cut(s) 160
BciT130I CCWGG 3 cut(s) 1503, 1643, 1787
BcnI CCSGG 1 cut(s) 1212
BcoDI GTCTC 2 cut(s) 416, 1514
BfaI CTAG 7 cut(s) 54, 381, 494, 572, 585, 1316, 1827
BfuAI ACCTGC 1 cut(s) 1459
BglII AGATCT 3 cut(s) 367, 1654, 1829
BisI GCNGC 2 cut(s) 201, 1527
BlsI GCNGC 2 cut(s) 202, 1528
Bme1390I CCNGG 4 cut(s) 1212, 1503, 1643, 1787
Bme18I GGWCC 4 cut(s) 884, 1076, 1439, 1789
BmgT120I GGNCC 5 cut(s) 40, 884, 1076, 1439, 1789
BmiI GGNNCC 4 cut(s) 1078, 1122, 1244, 1784
BmrFI CCNGG 4 cut(s) 1212, 1503, 1643, 1787
BmrI ACTGGG 4 cut(s) 394, 694, 753, 1014
BmsI GCATC 7 cut(s) 58, 84, 558, 775, 804, 1168, 1320
BmuI ACTGGG 4 cut(s) 394, 694, 753, 1014
BpiI GAAGAC 3 cut(s) 1529, 1557, 1845
BplI GAGNNNNNCTC 2 cut(s) 814, 846
BpmI CTGGAG 1 cut(s) 410
Bpu14I TTCGAA 2 cut(s) 816, 1488
BpuEI CTTGAG 1 cut(s) 545
BpuMI CCSGG 1 cut(s) 1212
BsaAI YACGTR 1 cut(s) 1723
BsaJI CCNNGG 2 cut(s) 1036, 1661
BsaXI ACNNNNNCTCC 4 cut(s) 1256, 1286, 1334, 1364
Bsc4I CCNNNNNNNGG 2 cut(s) 564, 1579
Bse1I ACTGG 4 cut(s) 400, 689, 748, 1009
Bse3DI GCAATG 2 cut(s) 674, 1461
BseBI CCWGG 3 cut(s) 1503, 1643, 1787
BseDI CCNNGG 2 cut(s) 1036, 1661
BseGI GGATG 8 cut(s) 73, 99, 118, 176, 573, 795, 1335, 1361
BseLI CCNNNNNNNGG 2 cut(s) 564, 1579
BseMI GCAATG 2 cut(s) 674, 1461
BseMII CTCAG 2 cut(s) 1229, 1544
BseNI ACTGG 4 cut(s) 400, 689, 748, 1009
BseXI GCAGC 2 cut(s) 187, 1538
BsgI GTGCAG 1 cut(s) 186
BshFI GGCC 3 cut(s) 41, 147, 190
BsiSI CCGG 2 cut(s) 866, 1212
BslFI GGGAC 1 cut(s) 1062
BslI CCNNNNNNNGG 2 cut(s) 564, 1579
BsmAI GTCTC 2 cut(s) 416, 1514
BsmFI GGGAC 1 cut(s) 1062
BsmI GAATGC 1 cut(s) 1496
BsnI GGCC 3 cut(s) 41, 147, 190
Bsp119I TTCGAA 2 cut(s) 816, 1488
Bsp1286I GDGCHC 1 cut(s) 832
Bsp1407I TGTACA 2 cut(s) 660, 1479
Bsp143I GATC 6 cut(s) 367, 899, 1214, 1242, 1654, 1829
Bsp19I CCATGG 1 cut(s) 1036
BspANI GGCC 3 cut(s) 41, 147, 190
BspCNI CTCAG 2 cut(s) 1230, 1543
BspLI GGNNCC 4 cut(s) 1078, 1122, 1244, 1784
BspMI ACCTGC 1 cut(s) 1459
BspPI GGATC 4 cut(s) 894, 1222, 1237, 1250
BspT104I TTCGAA 2 cut(s) 816, 1488
BsrDI GCAATG 2 cut(s) 674, 1461
BsrGI TGTACA 2 cut(s) 660, 1479
BsrI ACTGG 4 cut(s) 400, 689, 748, 1009
BssECI CCNNGG 2 cut(s) 1036, 1661
BssMI GATC 6 cut(s) 367, 899, 1214, 1242, 1654, 1829
BssSI CACGAG 1 cut(s) 1022
BssT1I CCWWGG 2 cut(s) 1036, 1661
Bst2BI CACGAG 1 cut(s) 1022
Bst2UI CCWGG 3 cut(s) 1503, 1643, 1787
Bst4CI ACNGT 6 cut(s) 153, 660, 1272, 1287, 1621, 1673
Bst6I CTCTTC 3 cut(s) 255, 1146, 1517
BstAUI TGTACA 2 cut(s) 660, 1479
BstBAI YACGTR 1 cut(s) 1723
BstBI TTCGAA 2 cut(s) 816, 1488
BstC8I GCNNGC 2 cut(s) 59, 251
BstDEI CTNAG 6 cut(s) 364, 371, 503, 563, 1238, 1530
BstDSI CCRYGG 1 cut(s) 1036
BstF5I GGATG 8 cut(s) 73, 99, 118, 176, 573, 795, 1335, 1361
BstKTI GATC 6 cut(s) 370, 902, 1217, 1245, 1657, 1832
BstMAI GTCTC 2 cut(s) 416, 1514
BstMBI GATC 6 cut(s) 367, 899, 1214, 1242, 1654, 1829
BstMWI GCNNNNNNNGC 2 cut(s) 17, 1451
BstNI CCWGG 3 cut(s) 1503, 1643, 1787
BstNSI RCATGY 4 cut(s) 667, 1349, 1816, 1846
BstSCI CCNGG 4 cut(s) 1210, 1501, 1641, 1785
BstV1I GCAGC 2 cut(s) 187, 1538
BstV2I GAAGAC 3 cut(s) 1529, 1557, 1845
BstX2I RGATCY 6 cut(s) 367, 899, 1214, 1242, 1654, 1829
BstYI RGATCY 6 cut(s) 367, 899, 1214, 1242, 1654, 1829
BsuRI GGCC 3 cut(s) 41, 147, 190
BtgI CCRYGG 1 cut(s) 1036
BtsCI GGATG 8 cut(s) 73, 99, 118, 176, 573, 795, 1335, 1361
BtsIMutI CAGTG 2 cut(s) 586, 1538
BveI ACCTGC 1 cut(s) 1459
Cac8I GCNNGC 2 cut(s) 59, 251
Cfr13I GGNCC 5 cut(s) 40, 884, 1076, 1439, 1789
CsiI ACCWGGT 2 cut(s) 1641, 1785
Csp6I GTAC 8 cut(s) 661, 682, 927, 1268, 1298, 1480, 1669, 1720
CviAII CATG 8 cut(s) 98, 664, 1037, 1049, 1116, 1346, 1813, 1843
CviQI GTAC 8 cut(s) 661, 682, 927, 1268, 1298, 1480, 1669, 1720
DdeI CTNAG 6 cut(s) 364, 371, 503, 563, 1238, 1530
DpnI GATC 6 cut(s) 369, 901, 1216, 1244, 1656, 1831
DpnII GATC 6 cut(s) 367, 899, 1214, 1242, 1654, 1829
DraI TTTAAA 2 cut(s) 315, 759
EaeI YGGCCR 2 cut(s) 145, 188
Eam1104I CTCTTC 3 cut(s) 255, 1146, 1517
EarI CTCTTC 3 cut(s) 255, 1146, 1517
Eco130I CCWWGG 2 cut(s) 1036, 1661
Eco24I GRGCYC 1 cut(s) 832
Eco32I GATATC 1 cut(s) 633
Eco47I GGWCC 4 cut(s) 884, 1076, 1439, 1789
Eco57I CTGAAG 2 cut(s) 1076, 1250
EcoO109I RGGNCCY 1 cut(s) 40
EcoRI GAATTC 2 cut(s) 304, 615
EcoRII CCWGG 3 cut(s) 1501, 1641, 1785
EcoRV GATATC 1 cut(s) 633
EcoT14I CCWWGG 2 cut(s) 1036, 1661
EcoT22I ATGCAT 2 cut(s) 99, 797
EcoT38I GRGCYC 1 cut(s) 832
ErhI CCWWGG 2 cut(s) 1036, 1661
FaeI CATG 8 cut(s) 101, 667, 1040, 1052, 1119, 1349, 1816, 1846
FaqI GGGAC 1 cut(s) 1062
FatI CATG 8 cut(s) 97, 663, 1036, 1048, 1115, 1345, 1812, 1842
FauNDI CATATG 1 cut(s) 1430
Fnu4HI GCNGC 2 cut(s) 201, 1527
FokI GGATG 8 cut(s) 80, 106, 125, 183, 580, 782, 1342, 1368
FriOI GRGCYC 1 cut(s) 832
Fsp4HI GCNGC 2 cut(s) 201, 1527
FspBI CTAG 7 cut(s) 54, 381, 494, 572, 585, 1316, 1827
GluI GCNGC 2 cut(s) 201, 1527
GsuI CTGGAG 1 cut(s) 410
HaeIII GGCC 3 cut(s) 41, 147, 190
HapII CCGG 2 cut(s) 866, 1212
Hin1II CATG 8 cut(s) 101, 667, 1040, 1052, 1119, 1349, 1816, 1846
HinfI GANTC 9 cut(s) 15, 50, 322, 499, 691, 907, 1303, 1465, 1498
HpaII CCGG 2 cut(s) 866, 1212
HphI GGTGA 2 cut(s) 751, 1612
Hpy166II GTNNAC 3 cut(s) 1002, 1412, 1603
Hpy188I TCNGA 6 cut(s) 310, 784, 1230, 1629, 1765, 1810
Hpy8I GTNNAC 3 cut(s) 1002, 1412, 1603
HpyAV CCTTC 8 cut(s) 290, 701, 763, 1051, 1177, 1287, 1355, 1713
HpyCH4III ACNGT 6 cut(s) 153, 660, 1272, 1287, 1621, 1673
HpyCH4IV ACGT 2 cut(s) 465, 1722
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 1451
HpyF3I CTNAG 6 cut(s) 364, 371, 503, 563, 1238, 1530
HpySE526I ACGT 2 cut(s) 465, 1722
Hsp92II CATG 8 cut(s) 101, 667, 1040, 1052, 1119, 1349, 1816, 1846
Kzo9I GATC 6 cut(s) 367, 899, 1214, 1242, 1654, 1829
LmnI GCTCC 3 cut(s) 1186, 1342, 1450
Lsp1109I GCAGC 2 cut(s) 187, 1538
LweI GCATC 7 cut(s) 58, 84, 558, 775, 804, 1168, 1320
MabI ACCWGGT 2 cut(s) 1641, 1785
MaeI CTAG 7 cut(s) 54, 381, 494, 572, 585, 1316, 1827
MaeII ACGT 2 cut(s) 465, 1722
MaeIII GTNAC 3 cut(s) 739, 955, 1287
MalI GATC 6 cut(s) 369, 901, 1216, 1244, 1656, 1831
MboI GATC 6 cut(s) 367, 899, 1214, 1242, 1654, 1829
MfeI CAATTG 2 cut(s) 551, 1544
MflI RGATCY 6 cut(s) 367, 899, 1214, 1242, 1654, 1829
MhlI GDGCHC 1 cut(s) 832
MlsI TGGCCA 1 cut(s) 190
MluNI TGGCCA 1 cut(s) 190
MlyI GAGTC 3 cut(s) 24, 700, 1459
MmeI TCCRAC 5 cut(s) 580, 1560, 1788, 1816, 1833
MnlI CCTC 8 cut(s) 53, 434, 520, 694, 730, 1233, 1258, 1765
Mox20I TGGCCA 1 cut(s) 190
Mph1103I ATGCAT 2 cut(s) 99, 797
MscI TGGCCA 1 cut(s) 190
MseI TTAA 8 cut(s) 179, 314, 356, 486, 758, 1011, 1382, 1470
MslI CAYNNNNRTG 1 cut(s) 1022
Msp20I TGGCCA 1 cut(s) 190
MspI CCGG 2 cut(s) 866, 1212
MspR9I CCNGG 4 cut(s) 1212, 1503, 1643, 1787
MunI CAATTG 2 cut(s) 551, 1544
Mva1269I GAATGC 1 cut(s) 1496
MvaI CCWGG 3 cut(s) 1503, 1643, 1787
MwoI GCNNNNNNNGC 2 cut(s) 17, 1451
NciI CCSGG 1 cut(s) 1212
NcoI CCATGG 1 cut(s) 1036
NdeI CATATG 1 cut(s) 1430
NdeII GATC 6 cut(s) 367, 899, 1214, 1242, 1654, 1829
NlaIII CATG 8 cut(s) 101, 667, 1040, 1052, 1119, 1349, 1816, 1846
NlaIV GGNNCC 4 cut(s) 1078, 1122, 1244, 1784
NmuCI GTSAC 2 cut(s) 739, 955
NsiI ATGCAT 2 cut(s) 99, 797
NspI RCATGY 4 cut(s) 667, 1349, 1816, 1846
NspV TTCGAA 2 cut(s) 816, 1488
PaqCI CACCTGC 1 cut(s) 1459
PciI ACATGT 2 cut(s) 1812, 1842
PcsI WCGNNNNNNNCGW 1 cut(s) 1029
PctI GAATGC 1 cut(s) 1496
PfeI GAWTC 6 cut(s) 50, 322, 499, 907, 1303, 1498
PflMI CCANNNNNTGG 1 cut(s) 1579
PfoI TCCNGGA 1 cut(s) 1501
PkrI GCNGC 2 cut(s) 202, 1528
PleI GAGTC 3 cut(s) 23, 699, 1459
PpsI GAGTC 3 cut(s) 23, 699, 1459
Ppu21I YACGTR 1 cut(s) 1723
PscI ACATGT 2 cut(s) 1812, 1842
Psp6I CCWGG 3 cut(s) 1501, 1641, 1785
PspGI CCWGG 3 cut(s) 1501, 1641, 1785
PspN4I GGNNCC 4 cut(s) 1078, 1122, 1244, 1784
PspPI GGNCC 5 cut(s) 40, 884, 1076, 1439, 1789
PsuI RGATCY 6 cut(s) 367, 899, 1214, 1242, 1654, 1829
RsaI GTAC 8 cut(s) 662, 683, 928, 1269, 1299, 1481, 1670, 1721
RsaNI GTAC 8 cut(s) 661, 682, 927, 1268, 1298, 1480, 1669, 1720
RseI CAYNNNNRTG 1 cut(s) 1022
SaqAI TTAA 8 cut(s) 179, 314, 356, 486, 758, 1011, 1382, 1470
SatI GCNGC 2 cut(s) 201, 1527
Sau3AI GATC 6 cut(s) 367, 899, 1214, 1242, 1654, 1829
Sau96I GGNCC 5 cut(s) 40, 884, 1076, 1439, 1789
SchI GAGTC 3 cut(s) 24, 700, 1459
ScrFI CCNGG 4 cut(s) 1212, 1503, 1643, 1787
SduI GDGCHC 1 cut(s) 832
SexAI ACCWGGT 2 cut(s) 1641, 1785
SfaNI GCATC 7 cut(s) 58, 84, 558, 775, 804, 1168, 1320
SfuI TTCGAA 2 cut(s) 816, 1488
SinI GGWCC 4 cut(s) 884, 1076, 1439, 1789
SmiI ATTTAAAT 1 cut(s) 315
SmiMI CAYNNNNRTG 1 cut(s) 1022
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
SspI AATATT 1 cut(s) 724
SspMI CTAG 7 cut(s) 54, 381, 494, 572, 585, 1316, 1827
StyD4I CCNGG 4 cut(s) 1210, 1501, 1641, 1785
StyI CCWWGG 2 cut(s) 1036, 1661
SwaI ATTTAAAT 1 cut(s) 315
TaaI ACNGT 6 cut(s) 153, 660, 1272, 1287, 1621, 1673
TaiI ACGT 2 cut(s) 468, 1725
TaqI TCGA 4 cut(s) 536, 816, 1488, 1593
TatI WGTACW 4 cut(s) 660, 681, 1479, 1668
TfiI GAWTC 6 cut(s) 50, 322, 499, 907, 1303, 1498
Tru1I TTAA 8 cut(s) 179, 314, 356, 486, 758, 1011, 1382, 1470
Tru9I TTAA 8 cut(s) 179, 314, 356, 486, 758, 1011, 1382, 1470
TscAI CASTG 2 cut(s) 586, 1538
TseFI GTSAC 2 cut(s) 739, 955
TseI GCWGC 2 cut(s) 200, 1526
Tsp45I GTSAC 2 cut(s) 739, 955
TspDTI ATGAA 8 cut(s) 248, 366, 999, 1118, 1215, 1412, 1695, 1791
TspGWI ACGGA 1 cut(s) 1235
TspRI CASTG 2 cut(s) 586, 1538
Van91I CCANNNNNTGG 1 cut(s) 1579
VpaK11BI GGWCC 4 cut(s) 884, 1076, 1439, 1789
XapI RAATTY 4 cut(s) 243, 285, 304, 615
XceI RCATGY 4 cut(s) 667, 1349, 1816, 1846
XcmI CCANNNNNNNNNTGG 1 cut(s) 1857
XspI CTAG 7 cut(s) 54, 381, 494, 572, 585, 1316, 1827
Zsp2I ATGCAT 2 cut(s) 99, 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.