Rroxscaffold_1G00041390

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0021329
Physical Location & Seq
Forward (+)
59302781 .. 59304332
1552 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_1G00041390.1

Sequence Viewer

Length: 339 bp
ATGGGTGTTACCGAGGACTGCCGCAATACATTCTTGGAACTGCAGAGAAAGAAGGCCTTTCGCTATGTGATTTTTAAGATTGATGAGAAGAAGAAAGAGGTTGTGGTAGAAAAGACCGGAGGCCCAGCTGAAAGTTATGATGATTTCACCGGTTCGTTGCCTGAAAATGATTGCCGATATGCAATCTATGACTTTGATTTTGTCACTTCAGAGAATTGCCGAAGAGCAAGATCTTCTTCATTGCATGCTCACTCAACAGCGAAAGTAAAGGTTGTTGTTGATGCGAAAGTAAAGGTTGTTGTTGATGCAACTTCGCTGGCATCTTCATTGTCATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

112

Amino Acids

12.53

Weight (kDa)

7.69

Isoelectric Point (pI)

34.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 2 - 74 1.5e-17 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021329)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0343061 RchiOBHm_Chr3g0490851
rosa_multiflora Rmu_sc0003545.1_g000013
rosa_roxburghii Rroxscaffold_1G00041390
rosa_samantha Rh1AG137600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 22
AcuI CTGAAG 1 cut(s) 192
AgeI ACCGGT 1 cut(s) 149
AluBI AGCT 1 cut(s) 128
AluI AGCT 1 cut(s) 128
AoxI GGCC 2 cut(s) 54, 121
ArsI GACNNNNNNTTYG 2 cut(s) 182, 214
AsiGI ACCGGT 1 cut(s) 149
AspS9I GGNCC 1 cut(s) 122
AsuHPI GGTGA 1 cut(s) 139
BfmI CTRYAG 1 cut(s) 41
BglII AGATCT 1 cut(s) 230
BisI GCNGC 1 cut(s) 22
BlsI GCNGC 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 122
BmsI GCATC 3 cut(s) 271, 295, 329
BsaJI CCNNGG 1 cut(s) 12
BsaWI WCCGGW 2 cut(s) 116, 149
Bse118I RCCGGY 1 cut(s) 149
Bse3DI GCAATG 1 cut(s) 239
BseDI CCNNGG 1 cut(s) 12
BseMI GCAATG 1 cut(s) 239
BseYI CCCAGC 1 cut(s) 124
BshFI GGCC 2 cut(s) 56, 123
BshTI ACCGGT 1 cut(s) 149
BsiSI CCGG 2 cut(s) 117, 150
BsnI GGCC 2 cut(s) 56, 123
Bsp143I GATC 1 cut(s) 230
BspACI CCGC 1 cut(s) 22
BspANI GGCC 2 cut(s) 56, 123
BspMAI CTGCAG 1 cut(s) 45
BspQI GCTCTTC 1 cut(s) 217
BsrDI GCAATG 1 cut(s) 239
BsrFI RCCGGY 1 cut(s) 149
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 1 cut(s) 230
Bst6I CTCTTC 1 cut(s) 217
BstC8I GCNNGC 2 cut(s) 246, 318
BstKTI GATC 1 cut(s) 233
BstMBI GATC 1 cut(s) 230
BstNSI RCATGY 1 cut(s) 248
BstSFI CTRYAG 1 cut(s) 41
BstX2I RGATCY 1 cut(s) 230
BstYI RGATCY 1 cut(s) 230
BsuRI GGCC 2 cut(s) 56, 123
Cac8I GCNNGC 2 cut(s) 246, 318
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 122
CspAI ACCGGT 1 cut(s) 149
CviAII CATG 1 cut(s) 245
CviJI RGCY 3 cut(s) 56, 123, 128
CviKI_1 RGCY 3 cut(s) 56, 123, 128
DpnI GATC 1 cut(s) 232
DpnII GATC 1 cut(s) 230
Eam1104I CTCTTC 1 cut(s) 217
EarI CTCTTC 1 cut(s) 217
Eco147I AGGCCT 1 cut(s) 56
Eco57I CTGAAG 1 cut(s) 192
FaeI CATG 1 cut(s) 248
FaiI YATR 6 cut(s) 66, 138, 180, 189, 246, 337
FalI AAGNNNNNCTT 4 cut(s) 41, 73, 220, 252
FatI CATG 1 cut(s) 244
Fnu4HI GCNGC 1 cut(s) 22
Fsp4HI GCNGC 1 cut(s) 22
GluI GCNGC 1 cut(s) 22
GsaI CCCAGC 1 cut(s) 128
HaeIII GGCC 2 cut(s) 56, 123
HapII CCGG 2 cut(s) 117, 150
Hin1II CATG 1 cut(s) 248
HpaII CCGG 2 cut(s) 117, 150
HphI GGTGA 1 cut(s) 139
Hpy188I TCNGA 1 cut(s) 211
HpyAV CCTTC 1 cut(s) 46
HpyCH4V TGCA 4 cut(s) 43, 182, 244, 308
Hsp92II CATG 1 cut(s) 248
Kzo9I GATC 1 cut(s) 230
LguI GCTCTTC 1 cut(s) 217
LpnPI CCDG 5 cut(s) 130, 138, 163, 174, 302
LweI GCATC 3 cut(s) 271, 295, 329
MaeIII GTNAC 2 cut(s) 7, 202
MalI GATC 1 cut(s) 232
MboI GATC 1 cut(s) 230
MboII GAAGA 6 cut(s) 100, 103, 225, 228, 234, 315
MflI RGATCY 1 cut(s) 230
MluCI AATT 1 cut(s) 214
MnlI CCTC 3 cut(s) 7, 91, 113
MseI TTAA 1 cut(s) 75
MspA1I CMGCKG 1 cut(s) 128
MspI CCGG 2 cut(s) 117, 150
NdeII GATC 1 cut(s) 230
NlaIII CATG 1 cut(s) 248
NmuCI GTSAC 1 cut(s) 202
NspI RCATGY 1 cut(s) 248
PaeI GCATGC 1 cut(s) 248
PceI AGGCCT 1 cut(s) 56
PciSI GCTCTTC 1 cut(s) 217
PinAI ACCGGT 1 cut(s) 149
PkrI GCNGC 1 cut(s) 23
PspFI CCCAGC 1 cut(s) 124
PspPI GGNCC 1 cut(s) 122
PstI CTGCAG 1 cut(s) 45
PsuI RGATCY 1 cut(s) 230
PvuII CAGCTG 1 cut(s) 128
SapI GCTCTTC 1 cut(s) 217
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 1 cut(s) 22
Sau3AI GATC 1 cut(s) 230
Sau96I GGNCC 1 cut(s) 122
SetI ASST 4 cut(s) 102, 130, 273, 297
SfaNI GCATC 3 cut(s) 271, 295, 329
SfcI CTRYAG 1 cut(s) 41
SgeI CNNG 9 cut(s) 25, 46, 129, 137, 162, 173, 240, 257, 329
SphI GCATGC 1 cut(s) 248
Sse9I AATT 1 cut(s) 214
SseBI AGGCCT 1 cut(s) 56
SsiI CCGC 1 cut(s) 22
StuI AGGCCT 1 cut(s) 56
TasI AATT 1 cut(s) 214
TauI GCSGC 1 cut(s) 24
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TseFI GTSAC 1 cut(s) 202
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 2 cut(s) 228, 315
XceI RCATGY 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.