Rroxscaffold_1G00069630

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0030938
Physical Location & Seq
Reverse (-)
90535974 .. 90538120
2147 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00069630.1

Sequence Viewer

Length: 687 bp
ATGAGCACTACCATCGAAACAATAGTCCCTAGTTTCGAGCAAGCCGACACTCCGCCCCCTTTCGAGACAACAAGATCGCAGGCACGCCACGGATCAACACTTGGAACAGAACAAGCGAAGGATGATGGTGGAGTTTTGGTGTCCAAACGAGCAAGTTTGAATTGGGTTTCTTCACCCGGAAATTCTAGCTACCGGTATGTTGGAATTTGGTATTCTCGGACTAGAGTATCTAATAAAACAGTGGTGTGGGTTGCAAACAGGAACAATCCCATCAATGATACCTCGGATGATAATAATGAAAACTTTTTATGGCAAAGTTTTGATCATCCCACGAATACTTTAATTCCGGGTATGAAAGTTGGGGTGAATTGGAAAACCGGGCAAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCCGCAATCGGGGACTATACCTTAAGGCTCTATCCAAATCAGATTGCATCCCCCGAAATTTTTATGTACAAGGGTTTGAGTAAGTATTGGCGAAGTCCGGGCCGGCGCCTAGTGTGGTCACTAATCAAGAGGAAACTTATTATCTCATGGATGACACCAAAGCAATTACAAGAGCAACAATGGATCGATTCGTGCACGAGCTCCTTACATGGGATGATGGTGGCCTTCAATGGAAGGAAGACTACTCGCACCGAATTCCCTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

228

Amino Acids

26.07

Weight (kDa)

9.46

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 95 - 136 5.2e-14 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030938)

Species Orthologous Gene IDs
rosa_laevigata RLG00000031283
rosa_roxburghii Rroxscaffold_1G00069630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 525
AciI CCGC 2 cut(s) 53, 423
AclWI GGATC 2 cut(s) 100, 611
AcsI RAATTY 4 cut(s) 181, 204, 477, 674
AcyI GRCGYC 1 cut(s) 526
AfaI GTAC 1 cut(s) 488
AfiI CCNNNNNNNGG 3 cut(s) 428, 429, 630
AflII CTTAAG 1 cut(s) 442
AgeI ACCGGT 1 cut(s) 192
AgsI TTSAA 2 cut(s) 160, 649
AluBI AGCT 2 cut(s) 189, 621
AluI AGCT 2 cut(s) 189, 621
Alw21I GWGCWC 3 cut(s) 8, 617, 623
Alw26I GTCTC 1 cut(s) 59
Alw44I GTGCAC 1 cut(s) 613
AlwI GGATC 2 cut(s) 100, 611
AoxI GGCC 2 cut(s) 520, 642
ApaLI GTGCAC 1 cut(s) 613
ApoI RAATTY 4 cut(s) 181, 204, 477, 674
ArsI GACNNNNNNTTYG 2 cut(s) 9, 41
AsiGI ACCGGT 1 cut(s) 192
AspLEI GCGC 1 cut(s) 528
AspS9I GGNCC 1 cut(s) 520
AsuC2I CCSGG 4 cut(s) 177, 348, 379, 519
AsuHPI GGTGA 2 cut(s) 165, 376
BaeGI GKGCMC 1 cut(s) 617
BaeI ACNNNNGTAYC 2 cut(s) 210, 243
BanI GGYRCC 1 cut(s) 525
BanII GRGCYC 1 cut(s) 623
BarI GAAGNNNNNNTAC 2 cut(s) 646, 678
BauI CACGAG 1 cut(s) 616
BbsI GAAGAC 1 cut(s) 665
Bbv12I GWGCWC 3 cut(s) 8, 617, 623
BccI CCATC 4 cut(s) 20, 119, 278, 631
BcgI CGANNNNNNTGC 1 cut(s) 29
BclI TGATCA 1 cut(s) 322
BcnI CCSGG 4 cut(s) 177, 348, 379, 519
BcoDI GTCTC 1 cut(s) 59
BfaI CTAG 4 cut(s) 30, 186, 222, 530
BfoI RGCGCY 1 cut(s) 529
BfrI CTTAAG 1 cut(s) 442
Bme1390I CCNGG 4 cut(s) 177, 348, 379, 519
BmgT120I GGNCC 1 cut(s) 520
BmiI GGNNCC 1 cut(s) 527
BmrFI CCNGG 4 cut(s) 177, 348, 379, 519
BmsI GCATC 1 cut(s) 476
BpiI GAAGAC 1 cut(s) 665
BpuMI CCSGG 4 cut(s) 177, 348, 379, 519
Bsa29I ATCGAT 1 cut(s) 606
BsaHI GRCGYC 1 cut(s) 526
BsaJI CCNNGG 2 cut(s) 88, 282
BsaWI WCCGGW 1 cut(s) 192
BsaXI ACNNNNNCTCC 2 cut(s) 123, 153
Bsc4I CCNNNNNNNGG 3 cut(s) 428, 429, 630
Bse118I RCCGGY 2 cut(s) 192, 522
BseCI ATCGAT 1 cut(s) 606
BseDI CCNNGG 2 cut(s) 88, 282
BseGI GGATG 6 cut(s) 127, 292, 325, 467, 576, 639
BseLI CCNNNNNNNGG 3 cut(s) 428, 429, 630
BseSI GKGCMC 1 cut(s) 617
BshFI GGCC 2 cut(s) 522, 644
BshNI GGYRCC 1 cut(s) 525
BshTI ACCGGT 1 cut(s) 192
BshVI ATCGAT 1 cut(s) 606
BsiHKAI GWGCWC 3 cut(s) 8, 617, 623
BsiSI CCGG 6 cut(s) 177, 193, 347, 378, 518, 523
BslFI GGGAC 2 cut(s) 11, 446
BslI CCNNNNNNNGG 3 cut(s) 428, 429, 630
BsmAI GTCTC 1 cut(s) 59
BsmFI GGGAC 2 cut(s) 11, 446
BsnI GGCC 2 cut(s) 522, 644
Bsp1286I GDGCHC 3 cut(s) 8, 617, 623
Bsp1407I TGTACA 1 cut(s) 486
Bsp143I GATC 4 cut(s) 74, 92, 322, 603
BspACI CCGC 2 cut(s) 53, 423
BspANI GGCC 2 cut(s) 522, 644
BspDI ATCGAT 1 cut(s) 606
BspLI GGNNCC 1 cut(s) 527
BspPI GGATC 2 cut(s) 100, 611
BspT107I GGYRCC 1 cut(s) 525
BspTI CTTAAG 1 cut(s) 442
BsrFI RCCGGY 2 cut(s) 192, 522
BsrGI TGTACA 1 cut(s) 486
BssAI RCCGGY 2 cut(s) 192, 522
BssECI CCNNGG 2 cut(s) 88, 282
BssMI GATC 4 cut(s) 74, 92, 322, 603
BssNI GRCGYC 1 cut(s) 526
BssSI CACGAG 1 cut(s) 616
Bst2BI CACGAG 1 cut(s) 616
Bst4CI ACNGT 1 cut(s) 241
BstACI GRCGYC 1 cut(s) 526
BstAFI CTTAAG 1 cut(s) 442
BstAUI TGTACA 1 cut(s) 486
BstC8I GCNNGC 4 cut(s) 42, 81, 85, 524
BstDSI CCRYGG 1 cut(s) 88
BstF5I GGATG 6 cut(s) 127, 292, 325, 467, 576, 639
BstH2I RGCGCY 1 cut(s) 529
BstHHI GCGC 1 cut(s) 528
BstKTI GATC 4 cut(s) 77, 95, 325, 606
BstMAI GTCTC 1 cut(s) 59
BstMBI GATC 4 cut(s) 74, 92, 322, 603
BstSCI CCNGG 4 cut(s) 175, 346, 377, 517
BstSLI GKGCMC 1 cut(s) 617
BstV2I GAAGAC 1 cut(s) 665
Bsu15I ATCGAT 1 cut(s) 606
BsuRI GGCC 2 cut(s) 522, 644
BsuTUI ATCGAT 1 cut(s) 606
BtgI CCRYGG 1 cut(s) 88
BtsCI GGATG 6 cut(s) 127, 292, 325, 467, 576, 639
BtsIMutI CAGTG 1 cut(s) 246
Cac8I GCNNGC 4 cut(s) 42, 81, 85, 524
CfoI GCGC 1 cut(s) 528
Cfr10I RCCGGY 2 cut(s) 192, 522
Cfr13I GGNCC 1 cut(s) 520
ClaI ATCGAT 1 cut(s) 606
Csp6I GTAC 1 cut(s) 487
CspAI ACCGGT 1 cut(s) 192
CviAII CATG 2 cut(s) 567, 629
CviJI RGCY 6 cut(s) 44, 189, 448, 522, 621, 644
CviKI_1 RGCY 6 cut(s) 44, 189, 448, 522, 621, 644
CviQI GTAC 1 cut(s) 487
DinI GGCGCC 1 cut(s) 527
DpnI GATC 4 cut(s) 76, 94, 324, 605
DpnII GATC 4 cut(s) 74, 92, 322, 603
EciI GGCGGA 1 cut(s) 42
Ecl136II GAGCTC 1 cut(s) 621
Eco24I GRGCYC 1 cut(s) 623
Eco53kI GAGCTC 1 cut(s) 621
EcoICRI GAGCTC 1 cut(s) 621
EcoRI GAATTC 1 cut(s) 674
EcoT38I GRGCYC 1 cut(s) 623
EgeI GGCGCC 1 cut(s) 527
EheI GGCGCC 1 cut(s) 527
FaeI CATG 2 cut(s) 570, 632
FaiI YATR 7 cut(s) 198, 310, 353, 438, 485, 568, 630
FaqI GGGAC 2 cut(s) 11, 446
FatI CATG 2 cut(s) 566, 628
FauI CCCGC 1 cut(s) 430
FbaI TGATCA 1 cut(s) 322
FokI GGATG 6 cut(s) 134, 299, 312, 454, 583, 646
FriOI GRGCYC 1 cut(s) 623
FspBI CTAG 4 cut(s) 30, 186, 222, 530
GlaI GCGC 1 cut(s) 527
HaeII RGCGCY 1 cut(s) 529
HaeIII GGCC 2 cut(s) 522, 644
HapII CCGG 6 cut(s) 177, 193, 347, 378, 518, 523
HhaI GCGC 1 cut(s) 528
Hin1I GRCGYC 1 cut(s) 526
Hin1II CATG 2 cut(s) 570, 632
Hin6I GCGC 1 cut(s) 526
HinP1I GCGC 1 cut(s) 526
HinfI GANTC 1 cut(s) 608
HpaII CCGG 6 cut(s) 177, 193, 347, 378, 518, 523
HphI GGTGA 2 cut(s) 165, 376
Hpy166II GTNNAC 1 cut(s) 615
Hpy188I TCNGA 3 cut(s) 219, 286, 462
Hpy188III TCNNGA 2 cut(s) 64, 547
Hpy8I GTNNAC 1 cut(s) 615
HpyAV CCTTC 3 cut(s) 112, 648, 655
HpyCH4III ACNGT 1 cut(s) 241
HpyCH4V TGCA 3 cut(s) 254, 467, 615
Hsp92I GRCGYC 1 cut(s) 526
Hsp92II CATG 2 cut(s) 570, 632
HspAI GCGC 1 cut(s) 526
KasI GGCGCC 1 cut(s) 525
KroI GCCGGC 1 cut(s) 522
KroNI GCCGGC 1 cut(s) 524
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 4 cut(s) 74, 92, 322, 603
LmnI GCTCC 1 cut(s) 626
LpnPI CCDG 8 cut(s) 65, 190, 206, 244, 360, 391, 531, 536
LweI GCATC 1 cut(s) 476
MaeI CTAG 4 cut(s) 30, 186, 222, 530
MaeIII GTNAC 2 cut(s) 408, 537
MalI GATC 4 cut(s) 76, 94, 324, 605
MboI GATC 4 cut(s) 74, 92, 322, 603
MboII GAAGA 2 cut(s) 162, 670
MhlI GDGCHC 3 cut(s) 8, 617, 623
MluCI AATT 8 cut(s) 160, 181, 204, 342, 367, 477, 584, 674
Mly113I GGCGCC 1 cut(s) 526
MmeI TCCRAC 1 cut(s) 181
MnlI CCTC 2 cut(s) 292, 543
MroNI GCCGGC 1 cut(s) 522
MseI TTAA 3 cut(s) 341, 395, 443
MspCI CTTAAG 1 cut(s) 442
MspI CCGG 6 cut(s) 177, 193, 347, 378, 518, 523
MspR9I CCNGG 4 cut(s) 177, 348, 379, 519
NaeI GCCGGC 1 cut(s) 524
NarI GGCGCC 1 cut(s) 526
NciI CCSGG 4 cut(s) 177, 348, 379, 519
NdeII GATC 4 cut(s) 74, 92, 322, 603
NgoMIV GCCGGC 1 cut(s) 522
NlaIII CATG 2 cut(s) 570, 632
NlaIV GGNNCC 1 cut(s) 527
NmuCI GTSAC 2 cut(s) 408, 537
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PdiI GCCGGC 1 cut(s) 524
PfeI GAWTC 1 cut(s) 608
PinAI ACCGGT 1 cut(s) 192
PluTI GGCGCC 1 cut(s) 529
Psp124BI GAGCTC 1 cut(s) 623
PspN4I GGNNCC 1 cut(s) 527
PspPI GGNCC 1 cut(s) 520
RsaI GTAC 1 cut(s) 488
RsaNI GTAC 1 cut(s) 487
SacI GAGCTC 1 cut(s) 623
SaqAI TTAA 3 cut(s) 341, 395, 443
Sau3AI GATC 4 cut(s) 74, 92, 322, 603
Sau96I GGNCC 1 cut(s) 520
ScrFI CCNGG 4 cut(s) 177, 348, 379, 519
SduI GDGCHC 3 cut(s) 8, 617, 623
SetI ASST 4 cut(s) 191, 284, 443, 623
SfaNI GCATC 1 cut(s) 476
SfoI GGCGCC 1 cut(s) 527
SmlI CTYRAG 1 cut(s) 442
SmoI CTYRAG 1 cut(s) 442
Sse9I AATT 8 cut(s) 160, 181, 204, 342, 367, 477, 584, 674
SsiI CCGC 2 cut(s) 53, 423
SspDI GGCGCC 1 cut(s) 525
SspMI CTAG 4 cut(s) 30, 186, 222, 530
SstI GAGCTC 1 cut(s) 623
StyD4I CCNGG 4 cut(s) 175, 346, 377, 517
TaaI ACNGT 1 cut(s) 241
TaqI TCGA 4 cut(s) 15, 36, 63, 606
TasI AATT 8 cut(s) 160, 181, 204, 342, 367, 477, 584, 674
TatI WGTACW 1 cut(s) 486
TfiI GAWTC 1 cut(s) 608
Tru1I TTAA 3 cut(s) 341, 395, 443
Tru9I TTAA 3 cut(s) 341, 395, 443
TscAI CASTG 1 cut(s) 246
TseFI GTSAC 2 cut(s) 408, 537
Tsp45I GTSAC 2 cut(s) 408, 537
TspDTI ATGAA 2 cut(s) 312, 368
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 1 cut(s) 246
Vha464I CTTAAG 1 cut(s) 442
VneI GTGCAC 1 cut(s) 613
XapI RAATTY 4 cut(s) 181, 204, 477, 674
XspI CTAG 4 cut(s) 30, 186, 222, 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.