Rroxscaffold_1G00073130

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0023135
Physical Location & Seq
Reverse (-)
94113998 .. 94115175
1178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00073130.1

Sequence Viewer

Length: 972 bp
ATGGCCAACGTCTGCCAAATTTCTTTACAGTGTGATGCTCTTCTTCTGTGCTTTTGGGATCCTTTCCTGGGAAGAAAGCATTACGTGAGCAAACTTCAAGACAACCTTGAAGCTATGGATTCTTCTTTGCGGGATTTAATTGCCTTGAAGAATGATATCAAGAGAAAGGTTGTGGTCGCTGAGCAGCAGCCACTAATGAAGTGCTTAAATCAAGTACATAATTGGATTTCAAGAGTCGAAATAGTAGAAGCACAAGTTGGTCAACTTGTCAATGAGGCTCCACAGCATTTTGAGAAGTTGTGCTGTATGGGTTACTGTTCAAAACAGTATATCTCAAGTTACAAGTATGGGAGAAGAGTGGCCCGACAATTGGTGGAAATTTATGACTTGAAAAACAAAGGAGAACTCATTGAAGAGGTGGCAGAAACCGTACCTGCGGATTTAGTTAATGAAATACCTCTAGAGCCCACTGTGGGAATGGAAGCTATGATTGATGAGATTTGGACCTACATTGAAGATGCAAATGTGAAAGTCATCGGCGTATATGGGATGGGAGGGGTGGGCAAAACCACCCTCCTTGCTAACATCTTTAACAGCTCCAAACACATTCTTCTTCCTTTCAATTATGTTCTATGGGTCACTATCTCCAGGGAGTTGAAGCTGGACAACATACAAGAAAAGATTGCGCGAGATGTTGGACTTATGAGTGACAAGTGGAAGGATCAATCTCGGCAAGAAAAGGCTCGAGACATCTTCGACAGCTTGGAAATGAAGTACTTGTCATTCACCTTGAAATCCCAAGGCTTGCTGAAGCGTAGCGAAAGAGTGGGGGGGTCTTCCACTTCTATTAAAAACGATCGGTCGAGCCATGTCTTCCAAGAAGACTCGCAAGAATGGAAACATGCGATTACAATCTTGCAGAAAGTTGCCTCAAAGTTCTCAAAAATGGGAGAGGAGGTGTTTCGATCCTAA

Protein Analysis

323

Amino Acids

36.98

Weight (kDa)

6.62

Isoelectric Point (pI)

54.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 161 - 258 3.9e-21 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023135)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0013603.1_g000019
rosa_roxburghii Rroxscaffold_1G00073130
rosa_samantha Rh5AG022500 Rh5CG024700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 442
AccII CGCG 1 cut(s) 688
AciI CCGC 2 cut(s) 130, 437
AclWI GGATC 4 cut(s) 53, 66, 729, 960
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 18, 378
AcuI CTGAAG 1 cut(s) 830
AfaI GTAC 3 cut(s) 216, 432, 776
AfiI CCNNNNNNNGG 3 cut(s) 68, 370, 473
AjnI CCWGG 2 cut(s) 66, 647
AjuI GAANNNNNNNTTGG 2 cut(s) 240, 272
AluBI AGCT 5 cut(s) 113, 485, 597, 661, 762
AluI AGCT 5 cut(s) 113, 485, 597, 661, 762
Alw26I GTCTC 1 cut(s) 741
AlwI GGATC 4 cut(s) 53, 66, 729, 960
Ama87I CYCGRG 1 cut(s) 744
AoxI GGCC 2 cut(s) 3, 360
ApeKI GCWGC 2 cut(s) 184, 187
ApoI RAATTY 2 cut(s) 18, 378
AspLEI GCGC 1 cut(s) 688
AspS9I GGNCC 2 cut(s) 361, 504
AsuHPI GGTGA 1 cut(s) 778
AvaI CYCGRG 1 cut(s) 744
AvaII GGWCC 1 cut(s) 504
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 58
BanII GRGCYC 1 cut(s) 468
BbsI GAAGAC 3 cut(s) 828, 865, 888
BbvI GCAGC 2 cut(s) 196, 199
BccI CCATC 1 cut(s) 544
BciT130I CCWGG 2 cut(s) 68, 649
BcoDI GTCTC 1 cut(s) 741
BfaI CTAG 1 cut(s) 461
BfuAI ACCTGC 1 cut(s) 442
BisI GCNGC 2 cut(s) 185, 188
BlpI GCTNAGC 1 cut(s) 180
BlsI GCNGC 2 cut(s) 186, 189
BmcAI AGTACT 1 cut(s) 776
Bme1390I CCNGG 2 cut(s) 68, 649
Bme18I GGWCC 1 cut(s) 504
BmeT110I CYCGRG 1 cut(s) 744
BmgT120I GGNCC 2 cut(s) 361, 504
BmiI GGNNCC 2 cut(s) 60, 279
BmrFI CCNGG 2 cut(s) 68, 649
BmsI GCATC 2 cut(s) 25, 508
BpiI GAAGAC 3 cut(s) 828, 865, 888
BpmI CTGGAG 1 cut(s) 631
Bpu1102I GCTNAGC 1 cut(s) 180
BpuEI CTTGAG 1 cut(s) 319
BsaAI YACGTR 1 cut(s) 85
BsaBI GATNNNNATC 1 cut(s) 911
BsaJI CCNNGG 3 cut(s) 67, 648, 799
BsaXI ACNNNNNCTCC 2 cut(s) 942, 972
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 370, 473
Bse8I GATNNNNATC 1 cut(s) 911
BseBI CCWGG 2 cut(s) 68, 649
BseDI CCNNGG 3 cut(s) 67, 648, 799
BseGI GGATG 1 cut(s) 555
BseJI GATNNNNATC 1 cut(s) 911
BseLI CCNNNNNNNGG 3 cut(s) 68, 370, 473
BseMII CTCAG 1 cut(s) 171
BseRI GAGGAG 1 cut(s) 968
BseXI GCAGC 2 cut(s) 196, 199
Bsh1236I CGCG 1 cut(s) 688
Bsh1285I CGRYCG 2 cut(s) 859, 863
BshFI GGCC 2 cut(s) 5, 362
BsiEI CGRYCG 2 cut(s) 859, 863
BsiHKCI CYCGRG 1 cut(s) 744
BslI CCNNNNNNNGG 3 cut(s) 68, 370, 473
BsmAI GTCTC 1 cut(s) 741
BsnI GGCC 2 cut(s) 5, 362
BsoBI CYCGRG 1 cut(s) 744
Bsp1286I GDGCHC 1 cut(s) 468
Bsp143I GATC 4 cut(s) 58, 721, 856, 965
Bsp1720I GCTNAGC 1 cut(s) 180
BspACI CCGC 2 cut(s) 130, 437
BspANI GGCC 2 cut(s) 5, 362
BspCNI CTCAG 1 cut(s) 172
BspFNI CGCG 1 cut(s) 688
BspLI GGNNCC 2 cut(s) 60, 279
BspMI ACCTGC 1 cut(s) 442
BspPI GGATC 4 cut(s) 53, 66, 729, 960
BspQI GCTCTTC 1 cut(s) 45
BssECI CCNNGG 3 cut(s) 67, 648, 799
BssMI GATC 4 cut(s) 58, 721, 856, 965
BssT1I CCWWGG 1 cut(s) 799
Bst2UI CCWGG 2 cut(s) 68, 649
Bst4CI ACNGT 5 cut(s) 30, 317, 327, 430, 472
Bst6I CTCTTC 3 cut(s) 45, 349, 408
BstBAI YACGTR 1 cut(s) 85
BstC8I GCNNGC 1 cut(s) 806
BstDEI CTNAG 1 cut(s) 180
BstF5I GGATG 1 cut(s) 555
BstFNI CGCG 1 cut(s) 688
BstHHI GCGC 1 cut(s) 688
BstKTI GATC 4 cut(s) 61, 724, 859, 968
BstMAI GTCTC 1 cut(s) 741
BstMBI GATC 4 cut(s) 58, 721, 856, 965
BstMCI CGRYCG 2 cut(s) 859, 863
BstNI CCWGG 2 cut(s) 68, 649
BstNSI RCATGY 1 cut(s) 905
BstSCI CCNGG 2 cut(s) 66, 647
BstUI CGCG 1 cut(s) 688
BstV1I GCAGC 2 cut(s) 196, 199
BstV2I GAAGAC 3 cut(s) 828, 865, 888
BstX2I RGATCY 1 cut(s) 58
BstYI RGATCY 1 cut(s) 58
BsuRI GGCC 2 cut(s) 5, 362
BtsCI GGATG 1 cut(s) 555
BtsIMutI CAGTG 2 cut(s) 35, 468
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 1 cut(s) 806
CfoI GCGC 1 cut(s) 688
Cfr13I GGNCC 2 cut(s) 361, 504
Csp6I GTAC 3 cut(s) 215, 431, 775
CspCI CAANNNNNGTGG 2 cut(s) 559, 594
CviAII CATG 2 cut(s) 869, 902
CviQI GTAC 3 cut(s) 215, 431, 775
DdeI CTNAG 1 cut(s) 180
DpnI GATC 4 cut(s) 60, 723, 858, 967
DpnII GATC 4 cut(s) 58, 721, 856, 965
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 45, 349, 408
EarI CTCTTC 3 cut(s) 45, 349, 408
Eco130I CCWWGG 1 cut(s) 799
Eco24I GRGCYC 1 cut(s) 468
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 504
Eco57I CTGAAG 1 cut(s) 830
Eco88I CYCGRG 1 cut(s) 744
EcoRII CCWGG 2 cut(s) 66, 647
EcoRV GATATC 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 799
EcoT38I GRGCYC 1 cut(s) 468
ErhI CCWWGG 1 cut(s) 799
FaeI CATG 2 cut(s) 872, 905
FalI AAGNNNNNCTT 2 cut(s) 90, 122
FatI CATG 2 cut(s) 868, 901
FauI CCCGC 1 cut(s) 123
Fnu4HI GCNGC 2 cut(s) 185, 188
FokI GGATG 1 cut(s) 562
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 2 cut(s) 185, 188
FspBI CTAG 1 cut(s) 461
GlaI GCGC 1 cut(s) 687
GluI GCNGC 2 cut(s) 185, 188
GsuI CTGGAG 1 cut(s) 631
HaeIII GGCC 2 cut(s) 5, 362
HhaI GCGC 1 cut(s) 688
Hin1II CATG 2 cut(s) 872, 905
Hin6I GCGC 1 cut(s) 686
HinP1I GCGC 1 cut(s) 686
HincII GTYRAC 1 cut(s) 263
HindII GTYRAC 1 cut(s) 263
HinfI GANTC 3 cut(s) 119, 234, 884
HphI GGTGA 1 cut(s) 778
Hpy166II GTNNAC 1 cut(s) 263
Hpy188III TCNNGA 5 cut(s) 98, 160, 231, 461, 746
Hpy8I GTNNAC 1 cut(s) 263
HpyAV CCTTC 1 cut(s) 712
HpyCH4III ACNGT 5 cut(s) 30, 317, 327, 430, 472
HpyCH4IV ACGT 2 cut(s) 9, 84
HpyCH4V TGCA 2 cut(s) 521, 919
HpyF3I CTNAG 1 cut(s) 180
HpySE526I ACGT 2 cut(s) 9, 84
Hsp92II CATG 2 cut(s) 872, 905
HspAI GCGC 1 cut(s) 686
Kzo9I GATC 4 cut(s) 58, 721, 856, 965
LguI GCTCTTC 1 cut(s) 45
LmnI GCTCC 2 cut(s) 283, 602
LpnPI CCDG 6 cut(s) 53, 80, 447, 634, 647, 661
Lsp1109I GCAGC 2 cut(s) 196, 199
LweI GCATC 2 cut(s) 25, 508
MaeI CTAG 1 cut(s) 461
MaeII ACGT 2 cut(s) 9, 84
MaeIII GTNAC 4 cut(s) 311, 338, 637, 707
MalI GATC 4 cut(s) 60, 723, 858, 967
MboI GATC 4 cut(s) 58, 721, 856, 965
MfeI CAATTG 1 cut(s) 368
MflI RGATCY 1 cut(s) 58
MhlI GDGCHC 1 cut(s) 468
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 18, 138, 220, 368, 378, 622
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 243, 878
MmeI TCCRAC 1 cut(s) 676
MnlI CCTC 8 cut(s) 268, 409, 468, 548, 584, 940, 946, 949
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 137, 206, 447, 591, 849
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 2 cut(s) 68, 649
MunI CAATTG 1 cut(s) 368
MvaI CCWGG 2 cut(s) 68, 649
MvnI CGCG 1 cut(s) 688
NdeII GATC 4 cut(s) 58, 721, 856, 965
NlaIII CATG 2 cut(s) 872, 905
NlaIV GGNNCC 2 cut(s) 60, 279
NmeAIII GCCGAG 1 cut(s) 709
NmuCI GTSAC 2 cut(s) 637, 707
NspI RCATGY 1 cut(s) 905
PaeR7I CTCGAG 1 cut(s) 744
PciSI GCTCTTC 1 cut(s) 45
PfeI GAWTC 1 cut(s) 119
PkrI GCNGC 2 cut(s) 186, 189
Ple19I CGATCG 1 cut(s) 859
PleI GAGTC 2 cut(s) 242, 878
PpsI GAGTC 2 cut(s) 242, 878
Ppu21I YACGTR 1 cut(s) 85
Psp6I CCWGG 2 cut(s) 66, 647
PspGI CCWGG 2 cut(s) 66, 647
PspN4I GGNNCC 2 cut(s) 60, 279
PspPI GGNCC 2 cut(s) 361, 504
PsuI RGATCY 1 cut(s) 58
PvuI CGATCG 1 cut(s) 859
RsaI GTAC 3 cut(s) 216, 432, 776
RsaNI GTAC 3 cut(s) 215, 431, 775
SapI GCTCTTC 1 cut(s) 45
SaqAI TTAA 5 cut(s) 137, 206, 447, 591, 849
SatI GCNGC 2 cut(s) 185, 188
Sau3AI GATC 4 cut(s) 58, 721, 856, 965
Sau96I GGNCC 2 cut(s) 361, 504
ScaI AGTACT 1 cut(s) 776
SchI GAGTC 2 cut(s) 243, 878
ScrFI CCNGG 2 cut(s) 68, 649
SduI GDGCHC 1 cut(s) 468
SfaNI GCATC 2 cut(s) 25, 508
Sfr274I CTCGAG 1 cut(s) 744
SinI GGWCC 1 cut(s) 504
SlaI CTCGAG 1 cut(s) 744
SmlI CTYRAG 2 cut(s) 334, 744
SmoI CTYRAG 2 cut(s) 334, 744
Sse9I AATT 6 cut(s) 18, 138, 220, 368, 378, 622
SsiI CCGC 2 cut(s) 130, 437
SspMI CTAG 1 cut(s) 461
StyD4I CCNGG 2 cut(s) 66, 647
StyI CCWWGG 1 cut(s) 799
TaaI ACNGT 5 cut(s) 30, 317, 327, 430, 472
TaiI ACGT 2 cut(s) 12, 87
TaqI TCGA 5 cut(s) 237, 745, 756, 863, 964
TaqII GACCGA 1 cut(s) 849
TasI AATT 6 cut(s) 18, 138, 220, 368, 378, 622
TatI WGTACW 2 cut(s) 214, 774
TfiI GAWTC 1 cut(s) 119
Tru1I TTAA 5 cut(s) 137, 206, 447, 591, 849
Tru9I TTAA 5 cut(s) 137, 206, 447, 591, 849
TscAI CASTG 2 cut(s) 35, 475
TseFI GTSAC 2 cut(s) 637, 707
TseI GCWGC 2 cut(s) 184, 187
Tsp45I GTSAC 2 cut(s) 637, 707
TspDTI ATGAA 3 cut(s) 212, 465, 785
TspRI CASTG 2 cut(s) 35, 475
VpaK11BI GGWCC 1 cut(s) 504
XapI RAATTY 2 cut(s) 18, 378
XbaI TCTAGA 1 cut(s) 460
XceI RCATGY 1 cut(s) 905
XcmI CCANNNNNNNNNTGG 1 cut(s) 475
XhoI CTCGAG 1 cut(s) 744
XspI CTAG 1 cut(s) 461
ZrmI AGTACT 1 cut(s) 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.