Rroxscaffold_2G00119330

cytochrome c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0020867
Physical Location & Seq
Forward (+)
51213669 .. 51214553
885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00119330.1

Sequence Viewer

Length: 885 bp
ATGGGCTTTGGCTTATGTAGATCAAAAATGATGAATGGGATTGTGGCACTCCACTCGCCGCCAATGCGGAAGGATGCCGCCGAAAAGAATGGAACGCTGCTTCGCTCTGCTGGATGCGTCGGATCCCGTACAACAAGCGAGCTTTTTACCCTCAAATTCAAACATGTGGGCGAAAAATGCTATCCTGCTCTATTGTTGCGTAGCAATAGAAGCCTGCTCATGCTGCTTCGGCGGCGCTTTTTCGCCTTCTCTTCGCTCTGGGCAGGAGCGCTAGTGGAGACGGGGAGGGAGCAGGCGAGGCGTGTCGTTCGTAATGGACAGAAAGATACCACCACTTCGCCTCTTTGTTGGACCGCCGGCGCGAACACAGTGGTCTCTGACCAAGACCAGGAACCAATTCGAATTTGGATCTTGACATGTCGGTGGTTTTTAACCGTAGGCATCTTGCCAGGAAGTTGGTGGGCTCATCATGAATTAGGTCGGGGTGGCTGGTGGTTTCGGGATCCCGTAGAAAATGCTTCTTTTATGCCTCGGGTATTAGCCACAGCTCGTATTCATTCAGTAATTCTACCCCTTCTTCATTCTTGGACCTCGTTTCTTAATATTGTGACTCTTCCATGCTGTGTCTCAGGAACCTTTTCAATACGGTCCGGATTGCTAGCTTCCGTTCATAGTTTTGCTACAGATGATACACGAGGGATCTTTTTATGGCGGTTCTTCCTTCTAATGACCGGCATATCTATGATTCTTTTCTCCCAGATGAAGCAGCAGGCATCGGTCCGTAGAACCTATAAAAAAGAGATGGTTGTGGCGCGAAGTACTCTTGTGCACCTACGTCACGCGGCTCGCGCGAAACCCCGCCCCGTTATGTTATGGAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_C_asm

Protein Analysis

294

Amino Acids

33.31

Weight (kDa)

11.09

Isoelectric Point (pI)

45.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_C_asm PF01578 132 - 218 4.4e-14 Cytochrome C assembly protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020867)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0258021 RchiOBHm_MTg0498851
rosa_multiflora Rmu_sc0001972.1_g000014
rosa_roxburghii Rroxscaffold_2G00119330
rosa_samantha Rh1BG017100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 5 cut(s) 362, 814, 842, 849, 851
AccIII TCCGGA 1 cut(s) 650
AciI CCGC 8 cut(s) 59, 67, 78, 232, 354, 712, 842, 859
AclWI GGATC 6 cut(s) 117, 130, 416, 497, 510, 707
AcsI RAATTY 2 cut(s) 155, 402
AfaI GTAC 2 cut(s) 130, 820
AfeI AGCGCT 1 cut(s) 270
AfiI CCNNNNNNNGG 1 cut(s) 388
AflIII ACRYGT 2 cut(s) 163, 416
AgsI TTSAA 2 cut(s) 160, 642
AjnI CCWGG 2 cut(s) 387, 448
AluBI AGCT 3 cut(s) 142, 548, 662
AluI AGCT 3 cut(s) 142, 548, 662
Alw21I GWGCWC 1 cut(s) 831
Alw26I GTCTC 3 cut(s) 272, 379, 631
Alw44I GTGCAC 1 cut(s) 827
AlwI GGATC 6 cut(s) 117, 130, 416, 497, 510, 707
Ama87I CYCGRG 1 cut(s) 531
Aor13HI TCCGGA 1 cut(s) 650
Aor51HI AGCGCT 1 cut(s) 270
ApaLI GTGCAC 1 cut(s) 827
ApeKI GCWGC 3 cut(s) 97, 223, 766
ApoI RAATTY 2 cut(s) 155, 402
Asp700I GAANNNNTTC 2 cut(s) 396, 637
AspLEI GCGC 5 cut(s) 237, 271, 362, 814, 851
AspS9I GGNCC 4 cut(s) 351, 588, 648, 778
AsuII TTCGAA 1 cut(s) 400
AsuNHI GCTAGC 1 cut(s) 658
AvaI CYCGRG 1 cut(s) 531
AvaII GGWCC 4 cut(s) 351, 588, 648, 778
BaeGI GKGCMC 1 cut(s) 831
BamHI GGATCC 2 cut(s) 122, 502
BanII GRGCYC 1 cut(s) 466
BauI CACGAG 1 cut(s) 693
Bbv12I GWGCWC 1 cut(s) 831
BbvI GCAGC 3 cut(s) 84, 210, 778
BccI CCATC 1 cut(s) 796
BcgI CGANNNNNNTGC 2 cut(s) 36, 70
BciT130I CCWGG 2 cut(s) 389, 450
BcoDI GTCTC 3 cut(s) 272, 379, 631
BfaI CTAG 2 cut(s) 272, 659
BfmI CTRYAG 1 cut(s) 681
BfoI RGCGCY 2 cut(s) 238, 272
BisI GCNGC 7 cut(s) 59, 78, 98, 224, 233, 767, 843
BlsI GCNGC 7 cut(s) 60, 79, 99, 225, 234, 768, 844
BmcAI AGTACT 1 cut(s) 820
Bme1390I CCNGG 2 cut(s) 389, 450
Bme18I GGWCC 4 cut(s) 351, 588, 648, 778
BmeT110I CYCGRG 1 cut(s) 531
BmgT120I GGNCC 4 cut(s) 351, 588, 648, 778
BmiI GGNNCC 4 cut(s) 124, 393, 504, 634
BmrFI CCNGG 2 cut(s) 389, 450
BmsI GCATC 4 cut(s) 64, 104, 450, 782
BmtI GCTAGC 1 cut(s) 662
Bpu14I TTCGAA 1 cut(s) 400
BsaI GGTCTC 1 cut(s) 379
BsaJI CCNNGG 1 cut(s) 530
BsaWI WCCGGW 1 cut(s) 650
BsaXI ACNNNNNCTCC 2 cut(s) 258, 288
Bsc4I CCNNNNNNNGG 1 cut(s) 388
Bse118I RCCGGY 2 cut(s) 356, 731
BseAI TCCGGA 1 cut(s) 650
BseBI CCWGG 2 cut(s) 389, 450
BseDI CCNNGG 1 cut(s) 530
BseGI GGATG 2 cut(s) 79, 119
BseLI CCNNNNNNNGG 1 cut(s) 388
BseMII CTCAG 1 cut(s) 642
BseSI GKGCMC 1 cut(s) 831
BseXI GCAGC 3 cut(s) 84, 210, 778
Bsh1236I CGCG 5 cut(s) 362, 814, 842, 849, 851
BsiHKAI GWGCWC 1 cut(s) 831
BsiHKCI CYCGRG 1 cut(s) 531
BsiSI CCGG 3 cut(s) 357, 651, 732
BslI CCNNNNNNNGG 1 cut(s) 388
BsmAI GTCTC 3 cut(s) 272, 379, 631
BsmBI CGTCTC 1 cut(s) 272
Bso31I GGTCTC 1 cut(s) 379
BsoBI CYCGRG 1 cut(s) 531
Bsp119I TTCGAA 1 cut(s) 400
Bsp1286I GDGCHC 2 cut(s) 466, 831
Bsp13I TCCGGA 1 cut(s) 650
Bsp143I GATC 5 cut(s) 20, 122, 408, 502, 699
BspACI CCGC 8 cut(s) 59, 67, 78, 232, 354, 712, 842, 859
BspCNI CTCAG 1 cut(s) 641
BspEI TCCGGA 1 cut(s) 650
BspFNI CGCG 5 cut(s) 362, 814, 842, 849, 851
BspHI TCATGA 1 cut(s) 469
BspLI GGNNCC 4 cut(s) 124, 393, 504, 634
BspOI GCTAGC 1 cut(s) 662
BspPI GGATC 6 cut(s) 117, 130, 416, 497, 510, 707
BspT104I TTCGAA 1 cut(s) 400
BspTNI GGTCTC 1 cut(s) 379
BsrFI RCCGGY 2 cut(s) 356, 731
BssAI RCCGGY 2 cut(s) 356, 731
BssECI CCNNGG 1 cut(s) 530
BssMI GATC 5 cut(s) 20, 122, 408, 502, 699
BssSI CACGAG 1 cut(s) 693
Bst2BI CACGAG 1 cut(s) 693
Bst2UI CCWGG 2 cut(s) 389, 450
Bst4CI ACNGT 3 cut(s) 370, 436, 648
Bst6I CTCTTC 2 cut(s) 256, 618
BstBI TTCGAA 1 cut(s) 400
BstC8I GCNNGC 7 cut(s) 140, 215, 294, 358, 660, 771, 847
BstDEI CTNAG 1 cut(s) 628
BstF5I GGATG 2 cut(s) 79, 119
BstFNI CGCG 5 cut(s) 362, 814, 842, 849, 851
BstH2I RGCGCY 2 cut(s) 238, 272
BstHHI GCGC 5 cut(s) 237, 271, 362, 814, 851
BstKTI GATC 5 cut(s) 23, 125, 411, 505, 702
BstMAI GTCTC 3 cut(s) 272, 379, 631
BstMBI GATC 5 cut(s) 20, 122, 408, 502, 699
BstMWI GCNNNNNNNGC 8 cut(s) 64, 177, 210, 223, 229, 232, 298, 848
BstNI CCWGG 2 cut(s) 389, 450
BstNSI RCATGY 2 cut(s) 167, 420
BstSCI CCNGG 2 cut(s) 387, 448
BstSFI CTRYAG 1 cut(s) 681
BstSLI GKGCMC 1 cut(s) 831
BstUI CGCG 5 cut(s) 362, 814, 842, 849, 851
BstV1I GCAGC 3 cut(s) 84, 210, 778
BstX2I RGATCY 4 cut(s) 122, 408, 502, 699
BstXI CCANNNNNNTGG 1 cut(s) 456
BstYI RGATCY 4 cut(s) 122, 408, 502, 699
BtsCI GGATG 2 cut(s) 79, 119
BtsIMutI CAGTG 1 cut(s) 375
Cac8I GCNNGC 7 cut(s) 140, 215, 294, 358, 660, 771, 847
CciI TCATGA 1 cut(s) 469
CfoI GCGC 5 cut(s) 237, 271, 362, 814, 851
Cfr10I RCCGGY 2 cut(s) 356, 731
Cfr13I GGNCC 4 cut(s) 351, 588, 648, 778
CpoI CGGWCCG 2 cut(s) 648, 778
CseI GACGC 1 cut(s) 106
Csp6I GTAC 2 cut(s) 129, 819
CspI CGGWCCG 2 cut(s) 648, 778
CviAII CATG 5 cut(s) 164, 220, 417, 470, 618
CviQI GTAC 2 cut(s) 129, 819
DdeI CTNAG 1 cut(s) 628
DpnI GATC 5 cut(s) 22, 124, 410, 504, 701
DpnII GATC 5 cut(s) 20, 122, 408, 502, 699
Eam1104I CTCTTC 2 cut(s) 256, 618
EarI CTCTTC 2 cut(s) 256, 618
Eco24I GRGCYC 1 cut(s) 466
Eco31I GGTCTC 1 cut(s) 379
Eco47I GGWCC 4 cut(s) 351, 588, 648, 778
Eco47III AGCGCT 1 cut(s) 270
Eco88I CYCGRG 1 cut(s) 531
EcoRII CCWGG 2 cut(s) 387, 448
EcoT38I GRGCYC 1 cut(s) 466
Esp3I CGTCTC 1 cut(s) 272
FaeI CATG 5 cut(s) 167, 223, 420, 473, 621
FatI CATG 5 cut(s) 163, 219, 416, 469, 617
FauI CCCGC 1 cut(s) 866
Fnu4HI GCNGC 7 cut(s) 59, 78, 98, 224, 233, 767, 843
FokI GGATG 2 cut(s) 86, 126
FriOI GRGCYC 1 cut(s) 466
Fsp4HI GCNGC 7 cut(s) 59, 78, 98, 224, 233, 767, 843
FspBI CTAG 2 cut(s) 272, 659
GlaI GCGC 5 cut(s) 236, 270, 361, 813, 850
GluI GCNGC 7 cut(s) 59, 78, 98, 224, 233, 767, 843
HaeII RGCGCY 2 cut(s) 238, 272
HapII CCGG 3 cut(s) 357, 651, 732
HgaI GACGC 1 cut(s) 106
HhaI GCGC 5 cut(s) 237, 271, 362, 814, 851
Hin1II CATG 5 cut(s) 167, 223, 420, 473, 621
Hin6I GCGC 5 cut(s) 235, 269, 360, 812, 849
HinP1I GCGC 5 cut(s) 235, 269, 360, 812, 849
HinfI GANTC 2 cut(s) 610, 745
HpaII CCGG 3 cut(s) 357, 651, 732
Hpy166II GTNNAC 1 cut(s) 829
Hpy188I TCNGA 2 cut(s) 122, 379
Hpy188III TCNNGA 5 cut(s) 412, 470, 500, 630, 651
Hpy8I GTNNAC 1 cut(s) 829
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 4 cut(s) 64, 256, 584, 731
HpyCH4III ACNGT 3 cut(s) 370, 436, 648
HpyCH4IV ACGT 1 cut(s) 835
HpyCH4V TGCA 1 cut(s) 829
HpyF10VI GCNNNNNNNGC 8 cut(s) 64, 177, 210, 223, 229, 232, 298, 848
HpyF3I CTNAG 1 cut(s) 628
HpySE526I ACGT 1 cut(s) 835
Hsp92II CATG 5 cut(s) 167, 223, 420, 473, 621
HspAI GCGC 5 cut(s) 235, 269, 360, 812, 849
Kpn2I TCCGGA 1 cut(s) 650
KroI GCCGGC 1 cut(s) 356
KroNI GCCGGC 1 cut(s) 358
Kzo9I GATC 5 cut(s) 20, 122, 408, 502, 699
LmnI GCTCC 2 cut(s) 266, 289
Lsp1109I GCAGC 3 cut(s) 84, 210, 778
LweI GCATC 4 cut(s) 64, 104, 450, 782
MaeI CTAG 2 cut(s) 272, 659
MaeII ACGT 1 cut(s) 835
MaeIII GTNAC 2 cut(s) 607, 836
MalI GATC 5 cut(s) 22, 124, 410, 504, 701
MboI GATC 5 cut(s) 20, 122, 408, 502, 699
MboII GAAGA 4 cut(s) 243, 569, 605, 709
MflI RGATCY 4 cut(s) 122, 408, 502, 699
MhlI GDGCHC 2 cut(s) 466, 831
MluCI AATT 6 cut(s) 155, 396, 402, 473, 564, 880
MlyI GAGTC 1 cut(s) 604
MmeI TCCRAC 2 cut(s) 100, 329
MnlI CCTC 7 cut(s) 161, 279, 291, 351, 540, 601, 689
MreI CGCCGGCG 1 cut(s) 356
MroI TCCGGA 1 cut(s) 650
MroNI GCCGGC 1 cut(s) 356
MroXI GAANNNNTTC 2 cut(s) 396, 637
MseI TTAA 2 cut(s) 431, 600
MslI CAYNNNNRTG 2 cut(s) 421, 740
MspI CCGG 3 cut(s) 357, 651, 732
MspR9I CCNGG 2 cut(s) 389, 450
MvaI CCWGG 2 cut(s) 389, 450
MvnI CGCG 5 cut(s) 362, 814, 842, 849, 851
MwoI GCNNNNNNNGC 8 cut(s) 64, 177, 210, 223, 229, 232, 298, 848
NaeI GCCGGC 1 cut(s) 358
NdeII GATC 5 cut(s) 20, 122, 408, 502, 699
NgoMIV GCCGGC 1 cut(s) 356
NheI GCTAGC 1 cut(s) 658
NlaIII CATG 5 cut(s) 167, 223, 420, 473, 621
NlaIV GGNNCC 4 cut(s) 124, 393, 504, 634
NmuCI GTSAC 2 cut(s) 607, 836
NspI RCATGY 2 cut(s) 167, 420
NspV TTCGAA 1 cut(s) 400
PagI TCATGA 1 cut(s) 469
PciI ACATGT 2 cut(s) 163, 416
PdiI GCCGGC 1 cut(s) 358
PdmI GAANNNNTTC 2 cut(s) 396, 637
PfeI GAWTC 1 cut(s) 745
PkrI GCNGC 7 cut(s) 60, 79, 99, 225, 234, 768, 844
PleI GAGTC 1 cut(s) 604
PpsI GAGTC 1 cut(s) 604
PscI ACATGT 2 cut(s) 163, 416
Psp6I CCWGG 2 cut(s) 387, 448
PspGI CCWGG 2 cut(s) 387, 448
PspN4I GGNNCC 4 cut(s) 124, 393, 504, 634
PspPI GGNCC 4 cut(s) 351, 588, 648, 778
PsuI RGATCY 4 cut(s) 122, 408, 502, 699
RsaI GTAC 2 cut(s) 130, 820
RsaNI GTAC 2 cut(s) 129, 819
RseI CAYNNNNRTG 2 cut(s) 421, 740
Rsr2I CGGWCCG 2 cut(s) 648, 778
RsrII CGGWCCG 2 cut(s) 648, 778
SaqAI TTAA 2 cut(s) 431, 600
SatI GCNGC 7 cut(s) 59, 78, 98, 224, 233, 767, 843
Sau3AI GATC 5 cut(s) 20, 122, 408, 502, 699
Sau96I GGNCC 4 cut(s) 351, 588, 648, 778
ScaI AGTACT 1 cut(s) 820
SchI GAGTC 1 cut(s) 604
ScrFI CCNGG 2 cut(s) 389, 450
SduI GDGCHC 2 cut(s) 466, 831
SetI ASST 9 cut(s) 144, 481, 550, 593, 638, 664, 791, 834, 838
SfaNI GCATC 4 cut(s) 64, 104, 450, 782
SfcI CTRYAG 1 cut(s) 681
SfuI TTCGAA 1 cut(s) 400
SgrAI CRCCGGYG 1 cut(s) 356
SinI GGWCC 4 cut(s) 351, 588, 648, 778
SmiMI CAYNNNNRTG 2 cut(s) 421, 740
Sse9I AATT 6 cut(s) 155, 396, 402, 473, 564, 880
SsiI CCGC 8 cut(s) 59, 67, 78, 232, 354, 712, 842, 859
SspI AATATT 1 cut(s) 604
SspMI CTAG 2 cut(s) 272, 659
StyD4I CCNGG 2 cut(s) 387, 448
TaaI ACNGT 3 cut(s) 370, 436, 648
TaiI ACGT 1 cut(s) 838
TaqI TCGA 1 cut(s) 400
TaqII GACCGA 1 cut(s) 766
TasI AATT 6 cut(s) 155, 396, 402, 473, 564, 880
TatI WGTACW 1 cut(s) 818
TauI GCSGC 4 cut(s) 61, 80, 235, 845
TfiI GAWTC 1 cut(s) 745
Tru1I TTAA 2 cut(s) 431, 600
Tru9I TTAA 2 cut(s) 431, 600
TscAI CASTG 1 cut(s) 375
TseFI GTSAC 2 cut(s) 607, 836
TseI GCWGC 3 cut(s) 97, 223, 766
Tsp45I GTSAC 2 cut(s) 607, 836
TspDTI ATGAA 6 cut(s) 47, 486, 545, 569, 659, 776
TspGWI ACGGA 2 cut(s) 655, 770
TspRI CASTG 1 cut(s) 375
VneI GTGCAC 1 cut(s) 827
VpaK11BI GGWCC 4 cut(s) 351, 588, 648, 778
XapI RAATTY 2 cut(s) 155, 402
XceI RCATGY 2 cut(s) 167, 420
XcmI CCANNNNNNNNNTGG 2 cut(s) 402, 456
XmnI GAANNNNTTC 2 cut(s) 396, 637
XspI CTAG 2 cut(s) 272, 659
ZrmI AGTACT 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.