Rroxscaffold_2G00119370

NADH dehydrogenase (quinone) activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0020868
Physical Location & Seq
Reverse (-)
51244890 .. 51246703
1814 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00119370.1

Sequence Viewer

Length: 654 bp
ATGGGTGTCCGGAAGTTGTCATTGTACACTCCCCCGGGTCGAAAGGCGGCGGAGGAAGGAGGAGGACACTGGGCGGGATCTATCAGCAGCGAATTCCCTATACAGATAGAGGCGCCTATCAAAAAGATACTCCGAAGGCTTCGGGATCGAGGTATCATTAGCCGAAGAAGACCCTGGCCAATCCACGTGGCCTGCTTGACGAACGTCAGCGACGGAGACATCGTAAATTGGTCCGCGGGCATCGCGATAAGTCCTCTGTCCTACTACAGGTGCCGCGACAACCTTTACCAAGTCCGAACGATTGTCGACCACCAGATCCGCTGGTCTGCAATATTCACCCTAGCCCACAAGCACAAATCCTCGGCGCGGAATATAATCCCAAAGTACTCCAAAGACTCAAATATAGTAAATCAAGAAGGTGGTAAGACCCTTGCAGAGTTCCCCAACAGCATAGAGCTGGGGAAGCTCGGACCCGGTCAAGATCCGAACAACAAGGAGCACTCAACTACTACCCCACCCGCATTAGTAGAGGCGCTTATTTACTACTGTACTGAGTTGGCCTTATCTTCTGCCACCGAGAATGATGATGACTCTGAGGCGGCGGAAGAGAAAGTTTCTTCGTCTTCATTGACTAGTCGTCTGCTCGAGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Intron_maturas2

Protein Analysis

217

Amino Acids

24.0

Weight (kDa)

8.4

Isoelectric Point (pI)

58.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Intron_maturas2 PF01348 35 - 130 4.5e-12 Type II intron maturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020868)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0312971
rosa_laevigata RLG00000000363
rosa_multiflora Rmu_sc0001942.1_g000018
rosa_roxburghii Rroxscaffold_2G00119370
rosa_rugosa RorugMtG0000700.1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 112, 270
AccI GTMKAC 1 cut(s) 306
AccII CGCG 4 cut(s) 236, 245, 276, 367
AccIII TCCGGA 1 cut(s) 9
AclWI GGATC 4 cut(s) 85, 153, 310, 476
AcoI YGGCCR 1 cut(s) 176
AcsI RAATTY 1 cut(s) 92
AcvI CACGTG 1 cut(s) 187
AcyI GRCGYC 1 cut(s) 113
AfaI GTAC 3 cut(s) 26, 386, 550
AfiI CCNNNNNNNGG 2 cut(s) 267, 366
AhdI GACNNNNNGTC 1 cut(s) 636
AhlI ACTAGT 1 cut(s) 632
AjnI CCWGG 1 cut(s) 173
AluBI AGCT 2 cut(s) 457, 466
AluI AGCT 2 cut(s) 457, 466
Alw21I GWGCWC 1 cut(s) 501
Alw26I GTCTC 1 cut(s) 210
AlwI GGATC 4 cut(s) 85, 153, 310, 476
Ama87I CYCGRG 2 cut(s) 34, 644
Aor13HI TCCGGA 1 cut(s) 9
AoxI GGCC 3 cut(s) 176, 189, 558
ApeKI GCWGC 1 cut(s) 87
ApoI RAATTY 1 cut(s) 92
AspLEI GCGC 3 cut(s) 115, 367, 535
AspS9I GGNCC 2 cut(s) 231, 470
AsuC2I CCSGG 3 cut(s) 35, 36, 474
AsuHPI GGTGA 1 cut(s) 328
AvaI CYCGRG 2 cut(s) 34, 644
AvaII GGWCC 2 cut(s) 231, 470
BalI TGGCCA 1 cut(s) 178
BanI GGYRCC 2 cut(s) 112, 270
BbrPI CACGTG 1 cut(s) 187
BbsI GAAGAC 2 cut(s) 175, 615
Bbv12I GWGCWC 1 cut(s) 501
BbvI GCAGC 1 cut(s) 99
BciT130I CCWGG 1 cut(s) 175
BcnI CCSGG 3 cut(s) 35, 36, 474
BcoDI GTCTC 1 cut(s) 210
BcuI ACTAGT 1 cut(s) 632
BfaI CTAG 2 cut(s) 341, 633
BfmI CTRYAG 1 cut(s) 265
BfoI RGCGCY 2 cut(s) 116, 536
BisI GCNGC 4 cut(s) 48, 88, 274, 600
BlsI GCNGC 4 cut(s) 49, 89, 275, 601
BmcAI AGTACT 1 cut(s) 386
Bme1390I CCNGG 4 cut(s) 35, 36, 175, 474
Bme18I GGWCC 2 cut(s) 231, 470
BmeRI GACNNNNNGTC 1 cut(s) 636
BmeT110I CYCGRG 2 cut(s) 34, 644
BmgT120I GGNCC 2 cut(s) 231, 470
BmiI GGNNCC 3 cut(s) 114, 272, 472
BmrFI CCNGG 4 cut(s) 35, 36, 175, 474
BmrI ACTGGG 1 cut(s) 79
BmsI GCATC 1 cut(s) 249
BmuI ACTGGG 1 cut(s) 79
BoxI GACNNNNGTC 1 cut(s) 203
BpiI GAAGAC 2 cut(s) 175, 615
BpuMI CCSGG 3 cut(s) 35, 36, 474
BsaAI YACGTR 1 cut(s) 187
BsaHI GRCGYC 1 cut(s) 113
BsaJI CCNNGG 5 cut(s) 33, 34, 173, 234, 360
BsaWI WCCGGW 1 cut(s) 9
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bsc4I CCNNNNNNNGG 2 cut(s) 267, 366
Bse1I ACTGG 1 cut(s) 74
BseAI TCCGGA 1 cut(s) 9
BseBI CCWGG 1 cut(s) 175
BseDI CCNNGG 5 cut(s) 33, 34, 173, 234, 360
BseLI CCNNNNNNNGG 2 cut(s) 267, 366
BseMII CTCAG 2 cut(s) 543, 585
BseNI ACTGG 1 cut(s) 74
BseRI GAGGAG 1 cut(s) 75
BseXI GCAGC 1 cut(s) 99
BseYI CCCAGC 1 cut(s) 457
Bsh1236I CGCG 4 cut(s) 236, 245, 276, 367
BshFI GGCC 3 cut(s) 178, 191, 560
BshNI GGYRCC 2 cut(s) 112, 270
BsiHKAI GWGCWC 1 cut(s) 501
BsiHKCI CYCGRG 2 cut(s) 34, 644
BsiSI CCGG 3 cut(s) 10, 35, 474
BslI CCNNNNNNNGG 2 cut(s) 267, 366
BsmAI GTCTC 1 cut(s) 210
BsnI GGCC 3 cut(s) 178, 191, 560
BsoBI CYCGRG 2 cut(s) 34, 644
Bsp1286I GDGCHC 1 cut(s) 501
Bsp13I TCCGGA 1 cut(s) 9
Bsp1407I TGTACA 1 cut(s) 24
Bsp143I GATC 4 cut(s) 77, 145, 315, 481
Bsp68I TCGCGA 1 cut(s) 245
BspANI GGCC 3 cut(s) 178, 191, 560
BspCNI CTCAG 2 cut(s) 544, 586
BspEI TCCGGA 1 cut(s) 9
BspFNI CGCG 4 cut(s) 236, 245, 276, 367
BspLI GGNNCC 3 cut(s) 114, 272, 472
BspPI GGATC 4 cut(s) 85, 153, 310, 476
BspT107I GGYRCC 2 cut(s) 112, 270
BsrGI TGTACA 1 cut(s) 24
BsrI ACTGG 1 cut(s) 74
BssECI CCNNGG 5 cut(s) 33, 34, 173, 234, 360
BssMI GATC 4 cut(s) 77, 145, 315, 481
BssNI GRCGYC 1 cut(s) 113
Bst2UI CCWGG 1 cut(s) 175
Bst4CI ACNGT 1 cut(s) 548
Bst6I CTCTTC 1 cut(s) 600
BstACI GRCGYC 1 cut(s) 113
BstAUI TGTACA 1 cut(s) 24
BstBAI YACGTR 1 cut(s) 187
BstC8I GCNNGC 2 cut(s) 193, 238
BstDEI CTNAG 3 cut(s) 552, 594, 651
BstDSI CCRYGG 1 cut(s) 234
BstENI CCTNNNNNAGG 1 cut(s) 265
BstFNI CGCG 4 cut(s) 236, 245, 276, 367
BstH2I RGCGCY 2 cut(s) 116, 536
BstHHI GCGC 3 cut(s) 115, 367, 535
BstKTI GATC 4 cut(s) 80, 148, 318, 484
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 4 cut(s) 77, 145, 315, 481
BstMWI GCNNNNNNNGC 2 cut(s) 242, 463
BstNI CCWGG 1 cut(s) 175
BstPAI GACNNNNGTC 1 cut(s) 203
BstSCI CCNGG 4 cut(s) 33, 34, 173, 472
BstSFI CTRYAG 1 cut(s) 265
BstUI CGCG 4 cut(s) 236, 245, 276, 367
BstV1I GCAGC 1 cut(s) 99
BstV2I GAAGAC 2 cut(s) 175, 615
BstX2I RGATCY 3 cut(s) 77, 315, 481
BstYI RGATCY 3 cut(s) 77, 315, 481
BsuRI GGCC 3 cut(s) 178, 191, 560
BtgI CCRYGG 1 cut(s) 234
BtgZI GCGATG 1 cut(s) 226
BtsIMutI CAGTG 1 cut(s) 67
BtuMI TCGCGA 1 cut(s) 245
Cac8I GCNNGC 2 cut(s) 193, 238
CfoI GCGC 3 cut(s) 115, 367, 535
Cfr13I GGNCC 2 cut(s) 231, 470
Cfr42I CCGCGG 1 cut(s) 237
Cfr9I CCCGGG 1 cut(s) 34
Csp6I GTAC 3 cut(s) 25, 385, 549
CspCI CAANNNNNGTGG 2 cut(s) 168, 203
CviJI RGCY 8 cut(s) 139, 162, 178, 191, 344, 457, 466, 560
CviKI_1 RGCY 8 cut(s) 139, 162, 178, 191, 344, 457, 466, 560
CviQI GTAC 3 cut(s) 25, 385, 549
DdeI CTNAG 3 cut(s) 552, 594, 651
DinI GGCGCC 1 cut(s) 114
DpnI GATC 4 cut(s) 79, 147, 317, 483
DpnII GATC 4 cut(s) 77, 145, 315, 481
DriI GACNNNNNGTC 1 cut(s) 636
EaeI YGGCCR 1 cut(s) 176
Eam1104I CTCTTC 1 cut(s) 600
Eam1105I GACNNNNNGTC 1 cut(s) 636
EarI CTCTTC 1 cut(s) 600
EciI GGCGGA 2 cut(s) 65, 617
Eco47I GGWCC 2 cut(s) 231, 470
Eco72I CACGTG 1 cut(s) 187
Eco88I CYCGRG 2 cut(s) 34, 644
EcoNI CCTNNNNNAGG 1 cut(s) 265
EcoRI GAATTC 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 173
EgeI GGCGCC 1 cut(s) 114
EheI GGCGCC 1 cut(s) 114
FaiI YATR 4 cut(s) 101, 374, 404, 452
FauI CCCGC 3 cut(s) 67, 229, 526
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 4 cut(s) 48, 88, 274, 600
Fsp4HI GCNGC 4 cut(s) 48, 88, 274, 600
FspBI CTAG 2 cut(s) 341, 633
GlaI GCGC 3 cut(s) 114, 366, 534
GluI GCNGC 4 cut(s) 48, 88, 274, 600
GsaI CCCAGC 1 cut(s) 461
HaeII RGCGCY 2 cut(s) 116, 536
HaeIII GGCC 3 cut(s) 178, 191, 560
HapII CCGG 3 cut(s) 10, 35, 474
HhaI GCGC 3 cut(s) 115, 367, 535
Hin1I GRCGYC 1 cut(s) 113
Hin6I GCGC 3 cut(s) 113, 365, 533
HinP1I GCGC 3 cut(s) 113, 365, 533
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 3 cut(s) 395, 590, 647
HpaII CCGG 3 cut(s) 10, 35, 474
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 2 cut(s) 27, 307
Hpy188I TCNGA 5 cut(s) 134, 296, 470, 486, 595
Hpy188III TCNNGA 5 cut(s) 10, 143, 244, 413, 479
Hpy8I GTNNAC 2 cut(s) 27, 307
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 3 cut(s) 50, 129, 410
HpyCH4III ACNGT 1 cut(s) 548
HpyCH4IV ACGT 2 cut(s) 186, 204
HpyCH4V TGCA 2 cut(s) 329, 434
HpyF10VI GCNNNNNNNGC 2 cut(s) 242, 463
HpyF3I CTNAG 3 cut(s) 552, 594, 651
HpySE526I ACGT 2 cut(s) 186, 204
Hsp92I GRCGYC 1 cut(s) 113
HspAI GCGC 3 cut(s) 113, 365, 533
KasI GGCGCC 1 cut(s) 112
Kpn2I TCCGGA 1 cut(s) 9
KspI CCGCGG 1 cut(s) 237
Kzo9I GATC 4 cut(s) 77, 145, 315, 481
LmnI GCTCC 1 cut(s) 496
Lsp1109I GCAGC 1 cut(s) 99
LweI GCATC 1 cut(s) 249
MaeI CTAG 2 cut(s) 341, 633
MaeII ACGT 2 cut(s) 186, 204
MalI GATC 4 cut(s) 79, 147, 317, 483
MboI GATC 4 cut(s) 77, 145, 315, 481
MboII GAAGA 6 cut(s) 177, 180, 558, 609, 615, 617
MflI RGATCY 3 cut(s) 77, 315, 481
MhlI GDGCHC 1 cut(s) 501
MlsI TGGCCA 1 cut(s) 178
MluCI AATT 2 cut(s) 92, 226
MluNI TGGCCA 1 cut(s) 178
Mly113I GGCGCC 1 cut(s) 113
MlyI GAGTC 2 cut(s) 389, 584
MnlI CCTC 9 cut(s) 46, 53, 56, 103, 143, 264, 370, 523, 589
Mox20I TGGCCA 1 cut(s) 178
MroI TCCGGA 1 cut(s) 9
MscI TGGCCA 1 cut(s) 178
Msp20I TGGCCA 1 cut(s) 178
MspA1I CMGCKG 2 cut(s) 236, 321
MspI CCGG 3 cut(s) 10, 35, 474
MspR9I CCNGG 4 cut(s) 35, 36, 175, 474
MvaI CCWGG 1 cut(s) 175
MvnI CGCG 4 cut(s) 236, 245, 276, 367
MwoI GCNNNNNNNGC 2 cut(s) 242, 463
NarI GGCGCC 1 cut(s) 113
NciI CCSGG 3 cut(s) 35, 36, 474
NdeII GATC 4 cut(s) 77, 145, 315, 481
NlaIV GGNNCC 3 cut(s) 114, 272, 472
NmeAIII GCCGAG 1 cut(s) 341
NruI TCGCGA 1 cut(s) 245
PaeR7I CTCGAG 1 cut(s) 644
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PflFI GACNNNGTC 1 cut(s) 474
PkrI GCNGC 4 cut(s) 49, 89, 275, 601
PleI GAGTC 2 cut(s) 389, 584
PluTI GGCGCC 1 cut(s) 116
PmaCI CACGTG 1 cut(s) 187
PmlI CACGTG 1 cut(s) 187
PpsI GAGTC 2 cut(s) 389, 584
Ppu21I YACGTR 1 cut(s) 187
PshAI GACNNNNGTC 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 173
PspCI CACGTG 1 cut(s) 187
PspFI CCCAGC 1 cut(s) 457
PspGI CCWGG 1 cut(s) 173
PspN4I GGNNCC 3 cut(s) 114, 272, 472
PspPI GGNCC 2 cut(s) 231, 470
PspXI VCTCGAGB 1 cut(s) 644
PsuI RGATCY 3 cut(s) 77, 315, 481
PsyI GACNNNGTC 1 cut(s) 474
RruI TCGCGA 1 cut(s) 245
RsaI GTAC 3 cut(s) 26, 386, 550
RsaNI GTAC 3 cut(s) 25, 385, 549
SacII CCGCGG 1 cut(s) 237
SalI GTCGAC 1 cut(s) 305
SatI GCNGC 4 cut(s) 48, 88, 274, 600
Sau3AI GATC 4 cut(s) 77, 145, 315, 481
Sau96I GGNCC 2 cut(s) 231, 470
ScaI AGTACT 1 cut(s) 386
SchI GAGTC 2 cut(s) 389, 584
ScrFI CCNGG 4 cut(s) 35, 36, 175, 474
SduI GDGCHC 1 cut(s) 501
SetI ASST 8 cut(s) 154, 189, 207, 272, 285, 421, 459, 468
SfaNI GCATC 1 cut(s) 249
SfcI CTRYAG 1 cut(s) 265
SfoI GGCGCC 1 cut(s) 114
Sfr274I CTCGAG 1 cut(s) 644
Sfr303I CCGCGG 1 cut(s) 237
SgrBI CCGCGG 1 cut(s) 237
SinI GGWCC 2 cut(s) 231, 470
SlaI CTCGAG 1 cut(s) 644
SmaI CCCGGG 1 cut(s) 36
SmlI CTYRAG 1 cut(s) 644
SmoI CTYRAG 1 cut(s) 644
SpeI ACTAGT 1 cut(s) 632
Sse9I AATT 2 cut(s) 92, 226
SspDI GGCGCC 1 cut(s) 112
SspI AATATT 1 cut(s) 333
SspMI CTAG 2 cut(s) 341, 633
StyD4I CCNGG 4 cut(s) 33, 34, 173, 472
TaaI ACNGT 1 cut(s) 548
TaiI ACGT 2 cut(s) 189, 207
TaqI TCGA 4 cut(s) 40, 148, 306, 645
TasI AATT 2 cut(s) 92, 226
TatI WGTACW 3 cut(s) 24, 384, 548
TauI GCSGC 3 cut(s) 50, 276, 602
TscAI CASTG 1 cut(s) 74
TseI GCWGC 1 cut(s) 87
TspDTI ATGAA 1 cut(s) 615
TspGWI ACGGA 1 cut(s) 228
TspMI CCCGGG 1 cut(s) 34
TspRI CASTG 1 cut(s) 74
Tth111I GACNNNGTC 1 cut(s) 474
VpaK11BI GGWCC 2 cut(s) 231, 470
XagI CCTNNNNNAGG 1 cut(s) 265
XapI RAATTY 1 cut(s) 92
XhoI CTCGAG 1 cut(s) 644
XmaI CCCGGG 1 cut(s) 34
XmiI GTMKAC 1 cut(s) 306
XspI CTAG 2 cut(s) 341, 633
ZrmI AGTACT 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.