Rroxscaffold_2G00129530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0025537
Physical Location & Seq
Forward (+)
65472795 .. 65486835
14041 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00129530.1

Sequence Viewer

Length: 495 bp
ATGGCTTTATGCTGGCGACATGATGGTGCTCATTATTATGGTGGAGACATTCTCTTTATTGAGCAGCTCGGTGTTATGCAAAATCTTATGATAGCTCTTGGACTCTCGATAAACCCGTCCCGACCAACTCCACTCGGGGCCCAACACGTGTTTTTTTGGGCCCCGCTCTTTCGTTCTCCTTCTTCCTCTTCTCGCTCTCCTTCTCTCTTCTCCATTCCCTATCGGCTGCTCACCCCGACCAAGCCGACCATCCCCAAACCTCATTCCTCCTCCTCCTTAATCTTCAACCCCTTCTCCACTCCCTATCGCCGGTTTCCAGTCACCACCACCGCACCCACCCTCTCAATCAAAGTCTACACCGCGGATCAAGATATCTCCTTACTTGTCTCCGGTAACGGCACGTCTCCGGCAACGGAAAATCCTCCTCCGGGTCGAATCGACGAAGAACATCCTAGGGTCCGAAGCTTGATTTACGGGTTTTCTTCATCGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

164

Amino Acids

18.02

Weight (kDa)

9.51

Isoelectric Point (pI)

58.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025537)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002541.1_g000003
rosa_roxburghii Rroxscaffold_1G00030340 Rroxscaffold_2G00129530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 166
AccI GTMKAC 1 cut(s) 354
AccII CGCG 1 cut(s) 362
AciI CCGC 4 cut(s) 164, 330, 360, 362
AclWI GGATC 1 cut(s) 372
AcvI CACGTG 1 cut(s) 148
AfiI CCNNNNNNNGG 2 cut(s) 309, 428
AflIII ACRYGT 2 cut(s) 145, 147
AgsI TTSAA 1 cut(s) 286
AjiI CACGTC 1 cut(s) 402
AluBI AGCT 3 cut(s) 67, 95, 465
AluI AGCT 3 cut(s) 67, 95, 465
Alw21I GWGCWC 1 cut(s) 31
Alw26I GTCTC 3 cut(s) 39, 391, 408
AlwI GGATC 1 cut(s) 372
Ama87I CYCGRG 1 cut(s) 134
AoxI GGCC 2 cut(s) 138, 159
ApaI GGGCCC 2 cut(s) 142, 163
ApeKI GCWGC 2 cut(s) 64, 226
AspA2I CCTAGG 1 cut(s) 452
AspS9I GGNCC 5 cut(s) 138, 139, 159, 160, 457
AsuC2I CCSGG 1 cut(s) 429
AsuHPI GGTGA 2 cut(s) 223, 313
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 1 cut(s) 457
AvrII CCTAGG 1 cut(s) 452
BaeGI GKGCMC 2 cut(s) 142, 163
BanII GRGCYC 2 cut(s) 142, 163
BbrPI CACGTG 1 cut(s) 148
Bbv12I GWGCWC 1 cut(s) 31
BbvI GCAGC 2 cut(s) 76, 213
BccI CCATC 2 cut(s) 17, 257
BceAI ACGGC 1 cut(s) 412
BcnI CCSGG 1 cut(s) 429
BcoDI GTCTC 3 cut(s) 39, 391, 408
BfaI CTAG 1 cut(s) 453
BisI GCNGC 2 cut(s) 65, 227
BlnI CCTAGG 1 cut(s) 452
BlsI GCNGC 2 cut(s) 66, 228
Bme1390I CCNGG 1 cut(s) 429
Bme18I GGWCC 1 cut(s) 457
BmeT110I CYCGRG 1 cut(s) 134
BmgBI CACGTC 1 cut(s) 402
BmgT120I GGNCC 5 cut(s) 138, 139, 159, 160, 457
BmiI GGNNCC 5 cut(s) 139, 140, 161, 162, 458
BmrFI CCNGG 1 cut(s) 429
BplI GAGNNNNNCTC 2 cut(s) 36, 68
BpuMI CCSGG 1 cut(s) 429
BsaAI YACGTR 1 cut(s) 148
BsaJI CCNNGG 2 cut(s) 360, 452
BsaWI WCCGGW 1 cut(s) 389
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bsc4I CCNNNNNNNGG 2 cut(s) 309, 428
Bse118I RCCGGY 1 cut(s) 309
Bse1I ACTGG 1 cut(s) 317
BseDI CCNNGG 2 cut(s) 360, 452
BseGI GGATG 2 cut(s) 249, 448
BseLI CCNNNNNNNGG 2 cut(s) 309, 428
BseNI ACTGG 1 cut(s) 317
BseRI GAGGAG 3 cut(s) 259, 262, 414
BseSI GKGCMC 2 cut(s) 142, 163
BseXI GCAGC 2 cut(s) 76, 213
Bsh1236I CGCG 1 cut(s) 362
BshFI GGCC 2 cut(s) 140, 161
BsiHKAI GWGCWC 1 cut(s) 31
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 4 cut(s) 310, 390, 407, 428
BslFI GGGAC 1 cut(s) 103
BslI CCNNNNNNNGG 2 cut(s) 309, 428
BsmAI GTCTC 3 cut(s) 39, 391, 408
BsmBI CGTCTC 1 cut(s) 408
BsmFI GGGAC 1 cut(s) 103
BsnI GGCC 2 cut(s) 140, 161
BsoBI CYCGRG 1 cut(s) 134
Bsp120I GGGCCC 2 cut(s) 138, 159
Bsp1286I GDGCHC 3 cut(s) 31, 142, 163
Bsp143I GATC 1 cut(s) 364
BspACI CCGC 4 cut(s) 164, 330, 360, 362
BspANI GGCC 2 cut(s) 140, 161
BspFNI CGCG 1 cut(s) 362
BspLI GGNNCC 5 cut(s) 139, 140, 161, 162, 458
BspPI GGATC 1 cut(s) 372
BsrBI CCGCTC 1 cut(s) 166
BsrFI RCCGGY 1 cut(s) 309
BsrI ACTGG 1 cut(s) 317
BssAI RCCGGY 1 cut(s) 309
BssECI CCNNGG 2 cut(s) 360, 452
BssMI GATC 1 cut(s) 364
BssT1I CCWWGG 1 cut(s) 452
Bst6I CTCTTC 2 cut(s) 193, 212
BstBAI YACGTR 1 cut(s) 148
BstC8I GCNNGC 1 cut(s) 14
BstDSI CCRYGG 1 cut(s) 360
BstF5I GGATG 2 cut(s) 249, 448
BstFNI CGCG 1 cut(s) 362
BstKTI GATC 1 cut(s) 367
BstMAI GTCTC 3 cut(s) 39, 391, 408
BstMBI GATC 1 cut(s) 364
BstSCI CCNGG 1 cut(s) 427
BstSLI GKGCMC 2 cut(s) 142, 163
BstUI CGCG 1 cut(s) 362
BstV1I GCAGC 2 cut(s) 76, 213
BsuRI GGCC 2 cut(s) 140, 161
BtgI CCRYGG 1 cut(s) 360
BtrI CACGTC 1 cut(s) 402
BtsCI GGATG 2 cut(s) 249, 448
Cac8I GCNNGC 1 cut(s) 14
Cfr10I RCCGGY 1 cut(s) 309
Cfr13I GGNCC 5 cut(s) 138, 139, 159, 160, 457
Cfr42I CCGCGG 1 cut(s) 363
CviAII CATG 1 cut(s) 20
CviJI RGCY 9 cut(s) 5, 67, 95, 140, 161, 226, 244, 465, 491
CviKI_1 RGCY 9 cut(s) 5, 67, 95, 140, 161, 226, 244, 465, 491
DpnI GATC 1 cut(s) 366
DpnII GATC 1 cut(s) 364
Eam1104I CTCTTC 2 cut(s) 193, 212
EarI CTCTTC 2 cut(s) 193, 212
Eco130I CCWWGG 1 cut(s) 452
Eco24I GRGCYC 2 cut(s) 142, 163
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 1 cut(s) 457
Eco72I CACGTG 1 cut(s) 148
Eco88I CYCGRG 1 cut(s) 134
EcoO109I RGGNCCY 2 cut(s) 138, 160
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 1 cut(s) 452
EcoT38I GRGCYC 2 cut(s) 142, 163
ErhI CCWWGG 1 cut(s) 452
Esp3I CGTCTC 1 cut(s) 408
FaeI CATG 1 cut(s) 23
FaiI YATR 5 cut(s) 10, 21, 39, 77, 89
FaqI GGGAC 1 cut(s) 103
FatI CATG 1 cut(s) 19
FauI CCCGC 1 cut(s) 171
FblI GTMKAC 1 cut(s) 354
Fnu4HI GCNGC 2 cut(s) 65, 227
FokI GGATG 2 cut(s) 236, 435
FriOI GRGCYC 2 cut(s) 142, 163
Fsp4HI GCNGC 2 cut(s) 65, 227
FspBI CTAG 1 cut(s) 453
GluI GCNGC 2 cut(s) 65, 227
HaeIII GGCC 2 cut(s) 140, 161
HapII CCGG 4 cut(s) 310, 390, 407, 428
Hin1II CATG 1 cut(s) 23
HindIII AAGCTT 1 cut(s) 463
HinfI GANTC 2 cut(s) 102, 435
HpaII CCGG 4 cut(s) 310, 390, 407, 428
HphI GGTGA 2 cut(s) 223, 313
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 1 cut(s) 461
Hpy188III TCNNGA 3 cut(s) 106, 120, 368
Hpy8I GTNNAC 1 cut(s) 355
Hpy99I CGWCG 1 cut(s) 443
HpyAV CCTTC 3 cut(s) 189, 210, 301
HpyCH4IV ACGT 2 cut(s) 147, 401
HpyCH4V TGCA 1 cut(s) 79
HpySE526I ACGT 2 cut(s) 147, 401
Hsp92II CATG 1 cut(s) 23
KspI CCGCGG 1 cut(s) 363
Kzo9I GATC 1 cut(s) 364
LpnPI CCDG 5 cut(s) 323, 330, 403, 420, 441
Lsp1109I GCAGC 2 cut(s) 76, 213
MaeI CTAG 1 cut(s) 453
MaeII ACGT 2 cut(s) 147, 401
MaeIII GTNAC 2 cut(s) 319, 392
MalI GATC 1 cut(s) 366
MbiI CCGCTC 1 cut(s) 166
MboI GATC 1 cut(s) 364
MboII GAAGA 6 cut(s) 174, 180, 199, 274, 455, 474
MhlI GDGCHC 3 cut(s) 31, 142, 163
MlyI GAGTC 1 cut(s) 96
MnlI CCTC 8 cut(s) 196, 270, 277, 280, 283, 350, 432, 435
MseI TTAA 2 cut(s) 278, 493
MslI CAYNNNNRTG 2 cut(s) 24, 36
MspA1I CMGCKG 1 cut(s) 362
MspI CCGG 4 cut(s) 310, 390, 407, 428
MspR9I CCNGG 1 cut(s) 429
MvnI CGCG 1 cut(s) 362
NciI CCSGG 1 cut(s) 429
NdeII GATC 1 cut(s) 364
NlaIII CATG 1 cut(s) 23
NlaIV GGNNCC 5 cut(s) 139, 140, 161, 162, 458
NmuCI GTSAC 1 cut(s) 319
PcsI WCGNNNNNNNCGW 1 cut(s) 113
PfeI GAWTC 1 cut(s) 435
PkrI GCNGC 2 cut(s) 66, 228
PleI GAGTC 1 cut(s) 96
PmaCI CACGTG 1 cut(s) 148
PmlI CACGTG 1 cut(s) 148
PpsI GAGTC 1 cut(s) 96
Ppu21I YACGTR 1 cut(s) 148
PspCI CACGTG 1 cut(s) 148
PspN4I GGNNCC 5 cut(s) 139, 140, 161, 162, 458
PspOMI GGGCCC 2 cut(s) 138, 159
PspPI GGNCC 5 cut(s) 138, 139, 159, 160, 457
RseI CAYNNNNRTG 2 cut(s) 24, 36
SacII CCGCGG 1 cut(s) 363
SaqAI TTAA 2 cut(s) 278, 493
SatI GCNGC 2 cut(s) 65, 227
Sau3AI GATC 1 cut(s) 364
Sau96I GGNCC 5 cut(s) 138, 139, 159, 160, 457
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 1 cut(s) 429
SduI GDGCHC 3 cut(s) 31, 142, 163
SetI ASST 6 cut(s) 69, 97, 150, 262, 404, 467
Sfr303I CCGCGG 1 cut(s) 363
SgrBI CCGCGG 1 cut(s) 363
SinI GGWCC 1 cut(s) 457
SmiMI CAYNNNNRTG 2 cut(s) 24, 36
SsiI CCGC 4 cut(s) 164, 330, 360, 362
SspMI CTAG 1 cut(s) 453
StyD4I CCNGG 1 cut(s) 427
StyI CCWWGG 1 cut(s) 452
TaiI ACGT 2 cut(s) 150, 404
TaqI TCGA 3 cut(s) 107, 433, 438
TfiI GAWTC 1 cut(s) 435
Tru1I TTAA 2 cut(s) 278, 493
Tru9I TTAA 2 cut(s) 278, 493
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 2 cut(s) 64, 226
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 1 cut(s) 474
TspGWI ACGGA 1 cut(s) 428
VpaK11BI GGWCC 1 cut(s) 457
XmaJI CCTAGG 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 354
XspI CTAG 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.